Instruction Manual for thermoscientific models including: Cryofuge 8 16 Centrifuge and Heavy Duty Centrifuge, Cryofuge 8 16, Centrifuge and Heavy Duty Centrifuge, Heavy Duty Centrifuge, Duty Centrifuge

50149611-k-EN - Thermo Scientific Cryofuge 8-16 Centrifuge and Heavy Duty Centrifuge Instruction Manual

50149611-k-EN - Thermo Scientific Cryofuge 8/16 Centrifuge and Heavy Duty Centrifuge Instruction Manual - Thermo Fisher Scientific

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Thermo Scientific Cryofuge 8 / 16 Centrifuge and Heavy Duty Centrifuge
Instruction Manual
50149611-k · 2022-02
Visit us online to register your warranty:
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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Table of Contents
Preface  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . vi
Items Supplied  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . vi Intended Use for Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty  .  .  .  .  .  .  .  .  .  .  .  . vii
Medical ........................................................................................................................... vii In Vitro Diagnostics .......................................................................................................... vii Signal Words and Colors  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . viii Precautions .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . viii Symbols used on the Centrifuge and its Components .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .xi Symbols used in the Manual  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .xiii
Transport and Set Up  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . I­1
1 . Location  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .I­1 2 . Transporting .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .I­2 3 . Setting Up  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .I­3
Setting Up...................................................................................................................... I­3 Level the Centrifuge ....................................................................................................... I­4 4 . Mains Connection  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .I­5 5 . Storage .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .I­5 6 . Shipping .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .I­6
Thermo Scientific Centri-Touch User Interface .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . II­1
Status  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . II­2 Run Parameters  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . II­3 Control and Configuration  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . II­3 Lighthouse Mode  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . II­4
Operation  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . III­1
1 . Switch on the Centrifuge  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .III­1 2 . Open the Centrifuge Door  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .III­1 3 . Install a Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .III­1
3. 1. Before Installation.................................................................................................III­2
i

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
3. 2. Rotor Installation ..................................................................................................III­3 4 . Maximum Loading .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .III­8
Use of Tubes and Consumables .....................................................................................III­9 5 . Close the Centrifuge Door .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .III­9 6 . Entering Parameters  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .III­9
6. 1. Acceleration / Deceleration Profiles .......................................................................III­9 6. 2. Preselect Speed / RCF-Value ................................................................................III­9 6. 3. Runtime Preselection..........................................................................................III­10 6. 4. Preselect Temperature ........................................................................................III­10 6. 5. Pre-warm or Pre-cool the Centrifuge ...................................................................III­10 7 . Programs  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . III­11 8 . Centrifugation  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . III­12 8. 1. Start ..................................................................................................................III­12 8. 2. Stop ..................................................................................................................III­12 8. 3. Using the Drain Box............................................................................................III­13 9 . Remove a Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . III­14 10 . Switch off the Centrifuge  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . III­15
Maintenance and Care  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­1
1 . Cleaning Intervals  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­1 2 . Basics .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­1
2. 1. Rotor and Components Inspection........................................................................ IV­2 3 . Cleaning  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­3
3. 1. Touchscreen ....................................................................................................... IV­4 3. 2. Filter Mat ............................................................................................................ IV­5 4 . Disinfection  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­6 5 . Decontamination  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­6 6 . Autoclaving  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­7 7 . Maintenance  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­8 7. 1. Preventive Maintenance....................................................................................... IV­8 7. 2. Service ............................................................................................................... IV­8 8 . Shipping and Disposal  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . IV­9
Troubleshooting  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . V-1
1 . Mechanical Emergency Door Release  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . V-1 2 . Ice Formation .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . V-2
ii

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
3 . Troubleshooting by Guide  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . V-3 4 . Information for the Customer Service  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . V-4
Technical Specifications  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . A­1
1 . Directives, Standards and Guidelines  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . A­2 2 . Refrigerants  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . A­3 3 . Mains Supply  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . A­3 4 . Components .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . A­4
Rotors  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­1
Items Supplied for Rotors  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­1 HAEMAFlex 6 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­2 HAEMAFlex 6 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­3 HAEMAFlex 6 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­4 HAEMAFlex 8 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­5 HAEMAFlex 8 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­6 HAEMAFlex 8 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­7 HAEMAFlex 12 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­8 HAEMAFlex 12 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­9 HAEMAFlex 16 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­10 HAEMAFlex 16 Rotor  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . B­11
Chemical Compatibility Chart  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . C­1
iii

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Table of Figures Figure I­1: Centrifuge clearance, example with bar code scanner ................................... I­2 Figure I­2: Position of the bubble in the water level........................................................ I­4 Figure I­3: Mains connection on the backside of the centrifuge ...................................... I­5 Figure III­1: Overview centrifuge and rotor, e.g. HAEMAFlex 12......................................III­2 Figure III­2: Align windshield knob and drive shaft .......................................................III­3 Figure III­3: Identify the circular marking, e.g. HAEMAFlex 8 and 12 .............................III­3 Figure III­4: Rotor locking tool ......................................................................................III­4 Figure III­5: Put the rotor locking tool............................................................................III­4 Figure III­6: Tighten the rotor to the drive shaft .............................................................III­5 Figure III­7: Lubricate trunnion bolts, e.g. HAEMAFlex 12 ..............................................III­5 Figure III­8: Install buckets into rotor, e.g. HAEMAFlex 12..............................................III­6 Figure III­9: Correctly assembled round bucket (75003836/75003936, not 75003964) .III­6 Figure III­10: Correct Loading.......................................................................................III­7 Figure III­11: Incorrect Loading.....................................................................................III­7 Figure III­12: Insert windshield lid into holder ...............................................................III­8 Figure III­13: Select program......................................................................................III­11 Figure III­14: Drain box (75007730) ...........................................................................III­13 Figure III­15: Installing the drain box (75007730)........................................................III­13 Figure III­16: Remove the plug ...................................................................................III­13 Figure III­17: Remove the rotor from the drive shaft. ...................................................III­14 Figure IV­1: Cleaning rotor bolts and bucket bearing surfaces ...................................... IV­4 Figure V­1: Marking on windshield lid ...........................................................................V-1 Figure V­2: Position of the mechanical emergency door release......................................V-2 Figure V­3: Example for an error message.....................................................................V-3
iv

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Table of Tables Table Preface­1: Items Supplied.......................................................................................vi Table Preface­2: Signal Words and Colors ...................................................................... viii Table Preface­3: Symbols used on the Centrifuge and its Components ............................ xiii Table Preface­4: Symbols used in the manual................................................................. xiii Table I­1: Overview of necessary tools for setting up...................................................... I­3 Table V­1: Error messages............................................................................................V-3 Table A­1: Technical Data Thermo Scientific Cryofuge 8 / 16 and Cryofuge 16 Heavy DutyA­1 Table A­2: Directives, Standards and Guidelines Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty ........................................................................................................................... A­2 Table A­3: Refrigerants used in the Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty..........A­3 Table A­4: Mains Supply..............................................................................................A­3 Table A­5: Components ...............................................................................................A­5 Table B­1: Items supplied for rotors..............................................................................B­1 Table B­2: HAEMAFlex 6 Rotor with Single Blood Bag Buckets (oval)..............................B­2 Table B­3: HAEMAFlex 6 Rotor with Single Blood Bag Buckets (round)...........................B­3 Table B­4: HAEMAFlex 6 Rotor with Single Blood Bag Buckets with Filter Pack...............B­4 Table B­5: HAEMAFlex 8 Rotor with Single Blood Bag Buckets (oval)..............................B­5 Table B­6: HAEMAFlex 8 Rotor with Single Blood Bag Buckets (round)...........................B­6 Table B­7: HAEMAFlex 8 Rotor with Single Blood Bag Buckets with Filter Pack...............B­7 Table B­8: HAEMAFlex 12 Rotor with Double Blood Bag Buckets ...................................B­8 Table B­9: HAEMAFlex 12 Rotor with Oval Buckets .......................................................B­9 Table B­10: HAEMAFlex 16 Rotor with Double Blood Bag Buckets ...............................B­10 Table B­11: HAEMAFlex 16 Rotor with Oval Buckets ...................................................B­11 Table C­1: Chemical Compatibility..............................................................................C­10
v

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Preface

Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers Warranty.

Items Supplied

The centrifuges are supplied without a rotor. The items supplied with a rotor are listed: [à2 B­1]

Article No . 75007671 75007672 75007673 75007674 75007678

Item
Centrifuge
Thermo Scientific Cryofuge 8, 200, 208, 220, 230, 240 V ±10 %, 50 / 60 Hz, Single phase
Thermo Scientific Cryofuge 8, 380, 400, 415 V ±10 %, 50 Hz, 3-phase
Thermo Scientific Cryofuge 16, 200, 208, 220, 230, 240 V ±10 %, 50 / 60 Hz, Single phase
Thermo Scientific Cryofuge 16, 380, 400, 415 V ±10 %, 50 Hz, 3-phase
Thermo Scientific Cryofuge 16 Heavy Duty, 380, 400, 415 V ±10 %, 50 Hz, 3-phase

20190357

Power Supply Cable For single phase units with 200, 208, 220, 230, 240 V IEC60309 32A-6h 3-pin blue, 200­250 V

Quantity 1
1

20190358

NEMA 6-30P 30A, 200­250 V

20190359 20190364

IEC60309 32A-6h 5-pin red (3P+N+PE), 220/380 V; 230/400 V; 240/415 V
NEMA L6-30P 30A, 200­205 V

20190360

For 3-phase units with 380/400/415 V
IEC60309 16A-6h 5 pin red (3P+N+PE), 220 / 380 V; 230 / 400 V; 240 / 415 V

20280119

Bubble Level

1

Instruction Manual

1

CD

1

Table Preface­1: Items Supplied If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.

vi

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Preface
Intended Use for Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty Medical
This centrifuge is intended for separation of blood into blood components using blood bags. The blood components are intended to be used for human blood transfusion. Clinical facilities and institutions are responsible for the process of human blood transfusion. This intended use is limited to the associated rotors and components. Based on the above this centrifuge is a medical device per European Directive 93/42/EEC. CE marked with Notified Body involvement.
In Vitro Diagnostics
This centrifuge system is intended for separation of blood into cell-free supernatant (plasma, serum) and cell-containing sediment. Plasma and serum are intended to be used in In-Vitro Diagnostic (IVD) applications (assays). Blood is used in numerous diagnostic tests such as hematological screening (e.g. measurement of free hemoglobulin), immunological screening (e.g. measurement of thrombocytes levels) and cardiovascular system assessment. Self declared, CE marked without Notified Body involvement.
Intended User
The centrifuge must be operated by trained personal only. Trained personnel can be a clinical laboratory technologist, a medical laboratory technician or personal with similar education.
Associated Rotors and Components
The Thermo ScientificTM Cryofuge 8 centrifuge can be operated with the Thermo ScientificTM HAEMAFlexTM 6 and HAEMAFlex 8 rotor. The Thermo Scientific Cryofuge 16 and Thermo Scientific Cryofuge 16 Heavy Duty centrifuge can be operated with the Thermo Scientific HAEMAFlex 12 and HAEMAFlex 16 rotor. For more details on buckets and further components: [à2 A­4] [à2 B­1]
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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Preface

Signal Words and Colors
Signal Word and Color

Degree of Hazard

WARNING Indicates a hazardous situation that, if not avoided, could result in death or serious injury.

CAUTION Indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.

NOTICE

Indicates information considered important, but not hazard-related.

Table Preface­2: Signal Words and Colors

Precautions

WARNING

Observe the safety instructions. Not following these instructions can cause damage like harm by mechanical impact, electrical shock, infection and loss of sample. The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with fatal consequences. The centrifuge must be operated by trained personnel only. It is the obligation of the operator to make sure, that the proper protective clothing is used. Mind the "Laboratory Biosafety Manual" of the World Health Organization (WHO) and the regulations in your country. As safety zone maintain a clear radius of at least 30 cm around the centrifuge. Do not place any dangerous substances within this safety zone. Set up in a well-ventilated environment, on a horizontally leveled and rigid surface with adequate load-bearing capacity. Do not modify the centrifuge and its accessories in any unauthorized way. The centrifuge housing is not to be opened by the operator.
Thermo Fisher Scientific is not responsible for the process of human blood transfusion.
To ensure safe operation of this centrifuge regarding blood and blood components you have to follow the regulations in your country.

WARNING

Risk of damage due to incorrect power supply.
Make sure that the centrifuge is plugged only into a power outlet that has been properly grounded.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Preface

WARNING

Risk from handling hazardous substances.
Especially when working with corrosive samples (salt solutions, acids, bases), the components and the centrifuge chamber have to be cleaned thoroughly.
Do not centrifuge explosive or flammable materials or substances.
The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment.
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions.
If centrifuging any hazardous materials mind the "Laboratory Biosafety Manual" of the World Health Organization (WHO) and any local regulations. When centrifuging microbiological samples from the Risk Group II (according to the "Laboratory Biosafety Manual" of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. Look on the internet page of the World Health Organization (www.who.int) for the "Laboratory Biosafety Manual". For materials in a higher risk group, extra safety measures have to be taken.
If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate disinfection measures have to be taken. [à2 IV­6]
Extreme care should be taken with highly corrosive substances which can cause damage and impair the mechanical stability of the rotor. These should only be centrifuged in fully sealed tubes.
If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.

WARNING

Serious injuries can occur, if you touch a spinning rotor with your hands or tools.
Never open the centrifuge door until the rotor has come to a complete stop and this has been confirmed on the touchscreen.
The emergency door release may be used in emergencies only to recover the samples from the centrifuge, e.g. during a power failure. [à2 V-1]
Do not open the centrifuge, while it is running.
In any case of severe mechanical failure, such as rotor or bucket crash, the centrifuge is not aerosol-tight.
In case of rotor failure the centrifuge can be damaged. Leave the room. Inform customer service.

WARNING

Safety can be impaired by wrong loading and worn accessories. Use only a properly installed rotor. [à2 III­1] Do not use rotors, buckets or components which show any signs of removed protective coating, corrosion or cracks. Contact customer service for further advice or inspections.
Use only with rotors which have been loaded properly.
Never overload the rotor.
Always balance the samples.
Use only rotors and components for this centrifuge which have been approved by Thermo Fisher Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge lab ware, provided they have been designed to fit the rotor or the adapter cavities and are approved for the speed or the RCF value of the rotor.
Make sure the rotor is locked properly into place before operating the centrifuge.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Preface

WARNING

Physical harm caused by ignoring operative basics. Never use the centrifuge if parts of its casing is damaged or missing. Never start the centrifuge when the centrifuge door is open. Do not move the centrifuge while it is running. Do not lean on the centrifuge. Do not place anything on top of the centrifuge during a run. Implement measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running.

WARNING

The magnets built into the rotors can have a negative effect on active implants, such as cardiac pacemakers.
The magnets are mounted on the bottom of the rotor.
Always keep a distance of 20 cm between the rotor and the active implant, as the product generates permanent magnetic fields. The magnetic field strength at a distance of 20 cm is less than 0.1 mT, so there should be no interference.

CAUTION

Due to air friction the temperature of rotor may rise significantly while the centrifuge is spinning.
Refrigerated units have limitations in cooling capabilities.
Displayed and set temperature can deviate from sample temperature. Sample temperature might exceed critical temperature of your application.

NOTICE

To shut down the centrifuge: Press the STOP key to shut down the centrifuge. Turn off the centrifuge at the power supply switch. The power supply plug must be freely accessible at all times. Pull out the power supply plug or disconnect the power supply in an emergency.

NOTICE

In case of serious incident:
Report any case of serious incident that has occured in relation to the device to the manufacturer and your local authorities

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Preface
Symbols used on the Centrifuge and its Components
This symbol refers to general hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol refers to hazards from sharp items. Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol refers to hazards from closing mechanical parts. Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol refers to information on hazards, described within the manual. Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol on rotors and components refers to information on hazards, described within the manual. Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol demands to disconnect mains before transporting or servicing the centrifuge.
This symbol shows the direction of rotation.
Indicates that the device is not sterile.
Indicates the device manufacturer.
Indicates the date when the device was manufactured.
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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Preface
Indicates the date after which the device is not to be used. Indicates the manufacturer's batch code so that the batch or lot can be identified. Indicates the manufacturer's catalogue number so that the device can be identified. Indicates the manufacturer's serial number so that a specific device can be identified. Indicates a device that is intended for one single use only. Indicates the need for the user to consult the instructions for use. Indicates a device that is intended to be used as an in vitro diagnostic medical device. Indicates a device as a medical device. The symbol indicating separate collection for EEE consists of the crossed-out wheeled bin. Indicates CE conformity. Indicates CE conformity controlled by a notified body. Indicates conformity to Chinese environmental law.
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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Preface

Indicates conformity with Underwriter Laboratories (UL) requirements.

Indicates the maximum possible weight of the adapter fully loaded.

Indicates the maximum rcf value for centrifugation.

Indicates the upper limit of temperature to which the product can be safely exposed to.

Sterilizable in a steam sterilizer (autoclave) at the temperature specified.

Not sterilizable in a steam sterilizer (autoclave).
Table Preface­3: Symbols used on the Centrifuge and its Components
Symbols used in the Manual
This symbol refers to general hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.

[à2 B­1]

This is a cross reference. The arrow stands for "refer to" or "see". The symbol in the middle stands for "page". The page number is stated at the end. In this example it is B­1. The first sign stands for the chapter, the second sign for the page within this chapter. Page numbers are placed at each bottom of a page.

Table Preface­4: Symbols used in the manual

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Transport and Set Up

THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.

I

Transport and Set Up

CAUTION

Make sure that the centrifuge and the packaging is not damaged. Speak to the shipping company and Thermo Fisher Scientific immediately, if damage is found.

1. Location

WARNING

Risk of impact by moving centrifuge. The centrifuge can crush into objects and persons in a radius of 30 cm when spinning. Keep a safety zone of 30 cm around the centrifuge for safe operation. Make sure that no one is in the safety zone while the centrifuge spins.

CAUTION

UV rays decrease the stability of plastics. Do not expose the centrifuge, rotors and plastic components to direct sunlight.

Operate the centrifuge only inside a room. The set-up location must fulfill these requirements:  Keep a safety zone of minimum 30 cm (Figure I­1 on page I­2, green area) around the centrifuge.
Persons and hazardous substances must be kept out of this safety zone while centrifuging.  The supporting structure must:
» be stable and free of resonance, » be applicable for horizontal setup of the centrifuge, » hold the weight of the centrifuge.  Do not expose the centrifuge to heat and strong sunlight.  The set-up location must be well-ventilated at all times.

THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. I­1

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Transport and Set Up
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.

Figure I­1: Centrifuge clearance, example with bar code scanner
2. Transporting

WARNING

Never stand before a moving centrifuge to stop it. If the centrifuge is moved on an uneven surface, it can get faster due to its own weight. An impact of the centrifuge can lead to serious injuries.

CAUTION

Always remove the rotor before you transport the centrifuge. If you do not remove the rotor you can damage the centrifuge drive or drive shaft.

NOTICE

Dispose the centrifuge packaging.

NOTICE

Assign a shipping company for the transport. Inform the customer service about the transport.

 Use a forklift to lift a centrifuge that is fixed on a palette.  Impact can damage the centrifuge.  Send the centrifuge upright and if possible in packaging.

THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. I­2

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Transport and Set Up
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
3. Setting Up

CAUTION

Do not push at the touchscreen. Due to the weight of the centrifuge it must be controlled by two or more people when rolling of the pallet. Do not stand in front of the centrifuge when rolling it down the ramp. The centrifuge has four steering casters that must be parallel, so that the centrifuge can be moved from the pallet.

Make sure that the items supplied are complete. [à2 vi] If the items supplied are not complete, please speak to Thermo Fisher Scientific.

Necessary Tools
Graphic

Item Wrench (30 mm)

Quantity 2

Socket wrench with nut (19 mm)

1

Screwdriver (torque T30)

1

Screwdriver (torque T20)

1

Hexagon key (hexagon 4 mm)

1

Hexagon key (hexagon 5 mm)

1

Cutter

1

Table I­1: Overview of necessary tools for setting up
Setting Up
1. Remove the wooden plank on the backside ot the pallet. The backside of the pallet is the side with the bevels. 2. Screw both rails on the bevels. Make sure that you position them not at the edge of the pallet. 3. Remove the metal pipes
a. Remove the 4 screws from the downside of the pallet. b. Lift the centrifuge by turning down the 4 centrifuge feet until the metal pipes can be removed. c. Remove the metal pipes. 4. Lower the centrifuge by lifting the 4 centrifuge feet. Make sure that the 4 centrifuge feet are entirely turned up to the bottom of the centrifuge. 5. Move the centrifuge from the pallet. 6. Move the centrifuge to the location, where you want to operate it. 7. Turn down the 4 centrifuge feet until all 4 casters are not in contact with the ground. All 4 centrifuge feet need to have firm contact to the ground. [à2 I­4]. The maximum height for setting up is 9.5 cm (3.7 inch) measured from the ground to the bottom of the centrifuge. 8. To fasten the 4 centrifuge feet you have to fasten their two lock nuts. The lower lock nut has to be fastened gently down against the foot. The upper lock nut has to be fastened up against the centrifuge bottom.

THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. I­3

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Transport and Set Up
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.

Level the Centrifuge

CAUTION

If you do not level the centrifuge, the centrifuge can crash because of imbalance. If you move the centrifuge, you must level it again. Do not move the centrifuge with a rotor attached to the drive shaft because damage can occur to the drive. Do not put anything below the centrifuge feet to level the centrifuge.

Level the centrifuge as follows: 1. Put the bubble level on the top of the drive shaft in the centrifugation chamber. 2. Adjust the centrifuge feet until the bubble in the level is fully in the circle mark. 3. Turn the drive shaft with the bubble level around a full turn.
If 50% of the bubble stays in the circle mark, the centrifuge is leveled. If more than 50% of the bubble is out of the circle mark, the centrifuge must be leveled again.

Excellent

Acceptable

Unacceptable

Figure I­2: Position of the bubble in the water level 4. To fasten the 4 centrifuge feet you have to fasten their two lock nuts. The lower lock nut has to be fastened gently down
against the foot. The upper lock nut has to be fastened up against the centrifuge bottom.

THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. I­4

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Transport and Set Up
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
4. Mains Connection

NOTICE

Connect the centrifuge into grounded electrical sockets only.

Figure I­3: Mains connection on the backside of the centrifuge 1. Turn off the power supply switch located on the right side (pull the switch handle towards the front of the centrifuge). 2. Make sure that the cable specification agrees with the safety standards of your country. 3. Make sure that the voltage and frequency are the same as the figures on the rating plate.

5. Storage

WARNING

When you remove the centrifuge and components from use, clean and if necessary, disinfect or decontaminate the full system. If you are not sure speak to the Thermo Fisher Scientific customer service.

 Before storing the centrifuge and the components it must be clean and if necessary disinfected and decontaminated. Centrifuge, rotors, buckets and components have to be fully dry before storage.
 Keep the centrifuge in a clean, dust-free location.  Keep the centrifuge on its rubber feet and not on its casters.  Do not store the centrifuge in direct sunlight.

THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. I­5

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Transport and Set Up
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
6. Shipping

WARNING

Before shipping the centrifuge and components you must clean and if necessary, disinfect or decontaminate the full system. If you are not sure speak to the Thermo Fisher Scientific customer service.

Before shipping the centrifuge:  The centrifuge must be clean and decontaminated.  You must confirm the decontamination with a decontamination certificate.

THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. I­6

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Thermo ScientificCentri-Touch User Interface

I

Thermo Scientific Centri-Touch User Interface



Actual status information



Set values



Settings and navigation

II­1

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Thermo ScientificCentri-Touch User Interface
Status
The upper part of the touchscreen displays the centrifuge status. The remaining time is indicated while centrifugation is in progress. The progress bar shows the actual phase of the centrifugation.



Status: In the Time mode, the remaining time for the centrifugation process is shown here. In the Hold mode, the elapsed time is displayed.



Progress: The curve diagram is divided into the sections acceleration, centrifugation and deceleration.



Speed: The current speed of the rotor is shown here.



Temperature: The temperature in the centrifugation chamber is shown here.

Status that can be displayed

Ready

Centrifugation can be started.

Door open Centrifuge door is open.

Door moving Door is automatically closing or opening.

Error

An error has occurred.

Canceled

Centrifugation has been stopped manually.

Complete

Centrifugation has been successfully completed.

Pretempering Pretempering has been successfully completed. completed

No rotor

No rotor is identified in the centrifuge.

II­2

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Thermo ScientificCentri-Touch User Interface
Run Parameters
The set points for centrifugation can be set in the parameterization window. Clicking one of these buttons will open a new window in which you can input the appropriate set point value.



Acceleration: Select an acceleration profile (Level 1-10).



Time: Select the duration for centrifugation and the time mode.



Deceleration: Select the deceleration profile (1-10).



Speed: Set the speed as rpm or RCF.



Temperature: Set the temperature (in °C) for the centrifugation chamber.



Program: Select a program for centrifugation. The previously selected program will always be displayed initially.

You can change the parameters once you have selected a program. Changing a parameter will cancel the

program.



Rotor: The rotor currently in use is displayed.

Control and Configuration

In this section you can start and halt centrifugation. You can also define settings, such as for the centrifugation programs. If you are not sure of the use of a button you can use the tooltip mode, which provides information about all of the operator control elements.



Start centrifugation: Centrifugation can be started using the current set values.



Open Door / Stop: When centrifugation starts, this button switches to Stop .



Configuration: The configuration menu is displayed when this button is clicked. This button is not active while

centrifugation is in progress.



Operator: In certain modes, touching the operator name opens a window for selecting other operators.



Tooltip Mode: This button is used to activate the tooltip mode. All functional elements are deactivated with this

mode. When an element is selected in the tooltip mode a field is displayed with information about that particular

element. If you press the tooltip button again, this mode is canceled.

II­3

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Thermo ScientificCentri-Touch User Interface
Lighthouse Mode
If no entry is made via the main screen in 30 seconds, the unit changes to lighthouse mode. In this mode, the status, current values and progress display are displayed larger.
The set value entry fields disappear and the progress display occupies their space. The time display is enlarged, so that the centrifugation status can be seen clearly from a greater distance. It is still possible to stop the centrifuge. Pressing on any area of the screen causes it to exit from lighthouse mode.
II­4

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation
II

Operation
For detailed instructions and information refer to the separate manual for the Thermo ScientificTM Centri-TouchTM User Interface.

1. Switch on the Centrifuge
Push the switch at the right side. The centrifuge checks its software. The touchscreen shows the Thermo Scientific logo while booting. When ready the touchscreen shows the current status of the centrifuge.

2. Open the Centrifuge Door
Press the Open button on the touchscreen. [à2 II­3] The centrifuge door will be opened automatically with the Thermo ScientificTM Auto-DoorTM function. The centrifuge door can only be opened when the centrifuge is switched on.

CAUTION

Do not use the mechanical emergency door release as regular procedure to open the centrifuge . Use the mechanical emergency door release, only if a malfunction or power failure occurs and only when you have made sure that the rotor stopped spinning. [à2 V-1]

3. Install a Rotor

WARNING

If you have not installed the rotor correctly, do not operate it. Make sure that the rotor is not damaged. Do not use damaged rotors. Make sure that the centrifugation chamber is free from objects.

NOTICE

Two persons are necessary for this procedure because of the weight of a rotor.

III­1

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation
3. 1. Before Installation
Make sure that all rotor parts are clean, dry and have no nicks and scratches. Always use a rotor body, a windshield bowl and a windshield lid that have the same article number. The windshield bowl and the rotor body of the HAEMAFlex 6 and 6 x 1000 mL Swinging Bucket Rotor are permantently connected and cannot be disassembled.



Windshield lid





Centrifugation chamber surface





Drive shaft





Guiding pin





Rotor hub

Figure III­1: Overview centrifuge and rotor, e.g. HAEMAFlex 12

Rotor body Windshield bowl Locating pin Windshield knob

III­2

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation

3. 2. Rotor Installation

CAUTION CAUTION

An an improper installation of the rotor can cause severe damage to the rotor and the centrifuge. Make sure the rotor is locked properly into place before operating the centrifuge. Use the rotor locking tool (20360104) clockwise to check if the rotor locking screw is tightened to the firm stop.
If you have not installed the rotor correctly, do not operate it. Make sure that the rotor is not damaged. Do not use damaged rotors. Make sure that the centrifugation chamber is free from objects.

1. Open the centrifuge door. If necessary remove dust and foreign objects from the chamber. Wipe the drive shaft with a clean cloth. Clean the threads of the drive shaft to prevent possible damage to rotor or centrifuge. Apply one drop of gresae (75003786) to the drive shaft. Wipe the rotor hub from the bottom side of the rotor with a clean cloth.
2. Hold the windshield bowl at the windshield knob with one hand and at the rim with the other hand. Put the windshield bowl carefully on the drive shaft. For some windshield bowls it may be easier to handle them with 2 people.
3. Align the square symbol on the windshield knob to the square end on the drive shaft.



Drive shaft



Windshield knob



Square symbol on windshield

knob

Figure III­2: Align windshield knob and drive shaft

4. Identify the circular marking on the rotor body.

Figure III­3: Identify the circular marking, e.g. HAEMAFlex 8 and 12 The hole for the locating pin of the windshield bowl is on the bottom side of the rotor body below the circular marking. Hold the rotor body over the drive shaft and align the circular marking to the locating pin on the windshield bowl. Put the rotor body carefully on the drive shaft. Turn the rotor body against the windshield bowl until the locating pin engages into the rotor body.
III­3

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation
CAUT I ON The centrifuge drive can be damaged, if the rotor body falls on it. If the rotor body is not engaged correctly it is not possible to turn the rotor locking screw into the drive shaft. Repeat step 3 and 4 until the rotor body is in position. 5. To prevent possible damage to rotor or centrifuge, clean the threads of the drive shaft.
Figure III­4: Rotor locking tool

Figure III­5: Put the rotor locking tool Put the rotor locking tool (20360104) into the opening of the guidung pin into the rotor locking screw. Use the rotor locking tool (20360104) clockwise to tighten the rotor with the rotor locking screw to the drive shaft.

CAUTION

An an improper installation of the rotor can cause severe damage to the rotor and the centrifuge. Make sure the rotor is locked properly into place before operating the centrifuge. Use the rotor locking tool (20360104) clockwise to check if the rotor locking screw is tightened to the firm stop.

NOTICE

With the locking screw tightened to a firm stop, by design intention, a small percebtable clearance remains between the rotor and the shaft.

III­4

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation
Figure III­6: Tighten the rotor to the drive shaft 6. Clean and lubricate the rotor trunnion bolts and the two bolt seats on the sides of each bucket [à2 IV­3]
To prevent possible damage to rotor or centrifuge, apply grease (75003786) to the rotor trunnion bolts. This must be done each time you install buckets. One small drop of grease is necessary for each rotor trunnion bolt. Example: each highlighted rotor trunnion bolt in the picture needs to be greased.
Figure III­7: Lubricate trunnion bolts, e.g. HAEMAFlex 12 7. Install an empty bucket in each position. Install buckets with the same weight class on opposing positions. Make sure
that each bucket swings out correctly. They must swing freely on their rotor trunnion bolts. CAUT I ON Always use a full complement of buckets, even if some stay empty.
III­5

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation

Figure III­8: Install buckets into rotor, e.g. HAEMAFlex 12 When assembling round buckets (75003836/75003936) insert the O-ring below the thread in the bucket. Do not insert it to the bucket lid. The bucket is assembled correctly, if the handles of the bucket lid are not aligned to the bolt seats of the bucket. If they are aligned to the bolt seats, the bucket is not closed correctly.

Figure III­9: Correctly assembled round bucket (75003836/75003936, not 75003964) 8. Load the buckets evenly. Balance opposite loads.

CAUTION

Incorrect loading can lead to damages. Always load the rotor symmetrically to avoid imbalance, bumpy spinning and possible damages. A full complement of buckets needs to be installed before operating the rotor.

III­6

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation
Correct Loading 
Figure III­10: Correct Loading
Incorrect Loading 
Figure III­11: Incorrect Loading 9. Put the windshield lid into the holder at the inner side of the centrifuge door. The Thermo ScientificTM Auto-LidTM function
automatically opens and closes the windshield when the centrifuge door opens and closes. The windshield lid has to be put into the holder at the inner side of the centrifuge door to correctly open and close the windshield.
III­7

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation

Figure III­12: Insert windshield lid into holder

CAUTION

Always put the windshield lid into the holder at the inner side of the centrifuge door. Make sure the rotor is locked properly. A missing windshield lid and an unlocked rotor can lead to severe damage of rotor and centrifuge.

4. Maximum Loading
Each rotor is designed to run with his maximum load at maximum speed. The safety system of the centrifuge requires that the rotor is not overloaded. The rotors are designed to work with substance mixtures with a density of up to 1.2 g/ml. If the admissible maximum load is exceeded the following steps need to be taken:  Reduce the fill level.  Reduce the speed. Use the table or the formula:

nadm = admissible maximum application speed nmax = maximum rated speed wmax = maximum rated load wapp = applied load
III­8

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation

Use of Tubes and Consumables
Care should be taken to ensure that the tubes and bottles used in the centrifuge are:  rated to or above the selected RCF to be spun at,  used at there minimum fill volume,  not used above their design life (age or number of runs),  undamaged. Please refer to manufacturers data sheets for further information.

5. Close the Centrifuge Door

WARNING

Do not grab into the clearance between the centrifuge door and the housing. The centrifuge door shuts automatically. Put your hands always on top of the centrifuge door. Use the emergency release only for malfunctions and electrical failures. [à2 V-1]

NOTICE

If the centrifuge door is open when the centrifuge is switched on, the centrifuge door needs to be closed manually. Only then, the centrifuge can be operated using the touchscreen.

Close the centrifuge door with the Auto-Door function by pressing on the Close button on the touchscreen. The centrifuge door will be automatically closed. Two locks close the centrifuge door fully. When closed, the touchscreen shows the Start button.
6. Entering Parameters

NOTICE

If the centrifuge door is open when the centrifuge is switched on, the centrifuge door needs to be closed manually. Only then, the centrifuge can be operated using the touchscreen.

6. 1. Acceleration / Deceleration Profiles
There are 10 curves (1-10) for acceleration and 10 for deceleration (1-10). The acceleration / deceleration profile can be selected in the main screen. Touch the number of the desired profile or move the slider by sliding your fingers over the numbers. Select Apply to confirm this selection for the next run. The profile with the lowest number has the shallowest incline marked with min; profile number 10 is the steepest incline which is marked with max.
6. 2. Preselect Speed / RCF-Value
1. Press on the speed field on the main screen. 2. Press rpm or RCF. The selected function is highlighted in yellow. 3. Enter the desired value. The digits show in sequential order. 4. Confirm your entry by pressing Confirm.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation

NOTICE

If a speed or RCF-value out of the nominal range has been selected, a message will appear with the permissible values.

Explanation of RCF-Value

The relative centrifugal force (RCF) is given as a multiple of the force of gravity (g). It is a unit less numerical value

which is used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of

the type of device. Only the centrifuging radius and the speed are used for calculation:

RCF = 11.18 x

x r r

r = centrifuging radius in cm

n = rotational speed in rpm

The maximum RCF value is related to the maximum radius of the tube opening.

Remember that this value is reduced depending on the tubes and buckets used.

This can be accounted for in the calculation above if required.

6. 3. Runtime Preselection

1. Press the time field on the main screen to open the runtime selection menu. 2. Press Time, Hold or  ²dt, depending on the value you would like to change. 3. Enter the desired value using the numeric pad. The digits show in sequential order. 4. Confirm your entry by pressing Confirm.

Time
Duration of centrifugation; input as hh:mm. The set time is counted down when centrifugation is in progress. Initial value: Defined duration hh:mm:00

Hold
Unlimited duration of centrifugation. The time elapsed so far is displayed while centrifugation is in progress. Initial value: 00:00:00

ACE
Accumulated Centrifugal Effect enter in x.y * 10z X: Whole digits (1st input field) Y: Decimal numbers (2nd input field) Z: Power (3rd input field)

6. 4. Preselect Temperature
You can preselect temperatures between -20 °C and 40 °C. To set the temperature, proceed as follows: 1. Press the temperature field in order to open the temperature menu. 2. Enter the desired temperature. The digits show in sequential order. 3. Confirm your entry by pressing Confirm.
6. 5. Pre-warm or Pre-cool the Centrifuge
To pre-temp the centrifuge, proceed as follows: 1. Use Configuration and Runs to select a stored program. The pre-temp function is connected to a program. 2. Press Load to select programmed parameters. In the main screen you can then set the required target temperature.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation
7. Programs
For detailed instructions and information refer to the according manual for the Thermo Scientific Centri-Touch User Interface. The instructions stated here are just an extract of how to work with programs. 1. Press on the touchscreen.
If a program is already loaded, the program name will be shown on the button.
Figure III­13: Select program 2. Press New.
a. Check the parameter. Change the parameter if necessary. To change a parameter, press the button of the parameter you want to change. Press Apply.
b. Press Enter Name. Enter a name for the program. Press Apply.
c. Press Select Rotor. Select a rotor. Press Apply. Select a bucket using the drop-down menu. Press Apply.
3. Press Apply to save the program. 4. Press the new created program.
Press Load. The program is now loaded and can be used.
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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation

8. Centrifugation
WARNI NG Do not operate the centrifuge on its casters.

CAUTION

An an improper installation of the rotor can cause severe damage to the rotor and the centrifuge. Make sure the rotor is locked properly into place before operating the centrifuge. Use the rotor locking tool (20360104) clockwise to check if the rotor locking screw is tightened to the firm stop.

8. 1. Start
Press the Start key on the touchscreen. The centrifuge accelerates to the preset speed with the time display active. After 30 seconds the touchscreen switches to the lighthouse mode. Pressing anywhere on the touchscreen ends the lighthouse mode. [à2 II­4] You cannot open the centrifuge door as long as the centrifuge is running.
8. 2. Stop
With preset Run Time
Usually the run time is preset and you only have to wait until the centrifuge stops automatically when the preset time limit expires. As soon as the speed drops to zero, the message Complete will appear on the touchscreen. Press Open Door to open the centrifuge door and remove the samples. You can also stop the centrifuging program manually at any time by pressing Stop . If the lighthouse mode is active, end it by pressing anywhere on the touchscreen. [à2 II­4]
Continuous Operation
If you selected continuous operation, you will have to stop the centrifuge manually. Press Stop  on the touchscreen. The centrifuge will be decelerated at the designated rate. If the lighthouse mode is active, end it by pressing anywhere on the touchscreen. [à2 II­4] The message Complete will appear on the touchscreen. Press Open Door to open the centrifuge door and remove the samples.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation
8. 3. Using the Drain Box
You can use the drain box (75007730) to collect water from of the centrifugation chamber.
Figure III­14: Drain box (75007730) 1. Install the drain box.

Figure III­15: Installing the drain box (75007730) 2. Remove the plug from the bottom of the centrifugation chamber.

Figure III­16: Remove the plug

NOTICE

Removing the plug and not installing the drain box will lead to spillage on the floor.

To remove the plug the centrifuge door must be open and the rotor including its windshield bowl has to be removed.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation

NOTICE

Don't use any sharp tool to extract the plug. This might damage the centrifugation chamber.

3. You have to regularly check, if the drain box is filled and empty the drain box to avoid spilling on the floor. 4. Clean, disinfect and decontaminate the drain box as stated in the Maintenance and Care chapter. [à2 IV­1]

9. Remove a Rotor

WARNING

Risk of cuts through fast rotating windshield lid. Do not open the centrifuge door while the rotor assembly spins. Only when the rotor assembly has stopped, use the mechanical emergency centrifuge door release.

NOTICE

For your protection when using an aerosol-tight rotor the bucket should only be removed with the lid closed.

NOTICE

Two people are necessary to remove the rotor because of its weight.

1. Open the centrifuge door. The centrifuge door lifts the windshield lid automatically.

The centrifuge door can only be opened when the centrifuge is switched on.

Press the Open Door button on the touchscreen. The centrifuge door will be automatically opened.

CAUTION

Do not use the mechanical emergency door release as regular procedure to open the centrifuge . Use the mechanical emergency door release, only if a malfunction or power failure occurs and only when you have made sure that the rotor stopped spinning. [à2 V-1]

2. Remove the windshield lid from the holder inside the centrifuge door. 3. Unload the buckets. 4. Remove the buckets. 5. Use the rotor locking tool (20360104) to disengage the rotor locking screw.

Figure III­17: Remove the rotor from the drive shaft. 6. Remove the rotor from the drive shaft.
CAUT I ON The centrifuge drive can be damaged if the rotor body falls on it. Hold the windshield knob with one hand and at the rim of the windshield bowl with the other. Lift the windshield bowl straight from the drive shaft. For some windshield bowls it may be easier to handle them with 2 persons.
III­14

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Operation
10. Switch off the Centrifuge
Turn off the power supply switch located on right side of the centrifuge.
III­15

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care
IV

Maintenance and Care

1. Cleaning Intervals
For the sake of personal, environmental and material protection, you must clean and if necessary, disinfect the centrifuge and its components on a regular basis.

2. Basics

CAUTION

Not rated procedures or agents could deteriorate the materials of the centrifuge and lead to malfunction. Refrain from using any other cleaning or decontamination procedure, if you are not entirely sure that the intended procedure is safe for the equipment. Use only cleaning agents that will not damage the equipment. In doubt contact the manufacturer of the cleaning agent. If in doubt, contact Thermo Fisher Scientific.

 Use warm water with a neutral detergent that is suitable for use with the materials. If in doubt contact the manufacturer of the cleaning agent.
 Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or scrubbing powder.  Remove rotor and clean centrifugation chamber with a small amount of cleaning agent on a clean cloth.  Use a soft brush without metal bristles to remove stubborn residue.
Afterwards rinse with a small amount of distilled water and remove any remains with absorbent towels.  Use only disinfectants with a pH of 6-8.

IV­1

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care

2. 1. Rotor and Components Inspection

CAUTION

Do not run any rotor or components with sign of damage. Ensure that the rotor, buckets and components are within the service life and number of cycles. It is recommend that you have rotors and components inspected yearly as part of your routine service to ensure safety.

After thoroughly cleaning the rotors, they must be inspected for damage, wear and corrosion. The cycle limits of the rotors are stated in the technical data section of each rotor. [à2 B­1] NOTICE Usage beyond these limits might lead to rotor failure, sample loss and damage to the centrifuge. The cycle limits of the buckets are stated on the buckets themselves and stated in the technical data section of each rotor. [à2 B­1] NOTICE Usage beyond these limits might lead to rotor failure, sample loss and damage to the centrifuge.
Metal Parts
Ensure that the protective coating is complete. It can be removed through wear and chemical attack and can lead to unseen corrosions. In case of corrosion, such as rust or white / metallic pitting, the rotor or components must be removed from service immediately. Particular attention should be paid to the bottom of buckets on swing out rotors and tube cavities on fixed angle rotors.
Plastic Parts
Check for signs of plastic crazing, fading, bruising or cracking. In case of damage, the inspected item must be removed from service immediately.
Cycles of Rotors and Buckets
The centrifuge counts cycles for a rotor type. The centrifuge does not count the cycles of the buckets. You have to count the cycles of the buckets using your own method. The centrifuge can not detect the change or replacement of rotors of the same type or buckets. The service lifetime of a rotor and its buckets depends on the amount of physical load. Do not use rotors and buckets that exceed the maximum number of cycles. The maximum number of cycles for rotors is given in the Rotors chapter. [à2 B­1] The maximum number of cycles for buckets is marked on the buckets themselves. You can check the number of cycles for a rotor type on the user interface of the centrifuge. The rotor log saves the information of the used rotors and buckets. Press Settings (gears symbol), then Runs and then Rotor Log to see the information. Besides the values of the rotor an information about the number of cycles completed by this rotor type in this centrifuge is shown. For detailed instructions and information refer to the separate manual for the Thermo Scientific Centri-Touch User Interface.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care

3. Cleaning

CAUTION

Before using any cleaning methods, users should check with the manufacturer of the cleaning agents that the proposed method will not damage the equipment.

CAUTION

Drive and door lock can be damaged by entering liquids. Do not allow liquids, especially organic solvents, to get on the drive shaft, the drive bearings or the centrifuge door locks. Organic solvents break down the grease in the motor bearing. The drive shaft could lock up.

Clean as follows: 1. Clean rotor, buckets and components outside of the centrifugation chamber. 2. Separate rotor, buckets, lids, adapters and tubes to allow thorough cleaning. 3. Rinse rotor and all components with warm water and a neutral detergent that is suitable for use with the materials. If in
doubt contact the manufacturer of the cleaning agent. Clean away the grease from the rotor trunnions (pivot point for swinging buckets). 4. Use a soft brush without metal bristles to remove stubborn residue. 5. Rinse rotor and all components with distilled water. 6. Place the rotors on a plastic grate with their cavities pointing down, to enable the cavities to fully drain and dry. 7. Dry all of the rotors and components after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 °C. If drying boxes are used, the temperature must never exceed 50 °C. Higher temperatures could damage the material and shorten the lifetime of the parts. 8. Inspect the rotor and components for signs of damages. [à2 IV­2] 9. After cleaning, treat the entire surface of aluminum parts including the cavities with corrosion protection oil (70009824). Treat the bolts of the swing out rotor with bolt grease (75003786).

IV­3

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care
Clean the bucket surfaces and rotor bolts as follows: a. Cut a cleaning pad (66309) into 12 mm (1/2 inch) wide strips. b. Saturate one of the pad strips with acetone or alcohol. c. Wrap the pad strip around a rotor bolt and move it back and forth in an oscillating motion. Move the strip around to
clean the trunnion bolt completely. Repeat this process for each trunnion bolt. d. Saturate another pad strip and thoroughly clean the bearing surfaces of each bucket.



Cleaning pad strip



Rotor bolt



Rotor body



Bucket



Bearing Surface

Figure IV­1: Cleaning rotor bolts and bucket bearing surfaces

e. Wipe the cleaned surfaces with a clean cloth.

f. After cleaning, treat the entire surface of aluminum parts including the cavities with corrosion protection oil (70009824).

Treat the bolts of the swing out rotor with bolt grease (75003786).

3. 1. Touchscreen
1. Pull out the power supply plug. 2. Clean the touchscreen using a dry microfiber cloth. 3. If necessary moisten the microfiber cloth and wipe the touchscreen again.

IV­4

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care

3. 2. Filter Mat

CAUTION

Do not touch the edges of the housing with your hands when the grid is removed. Cutting damage can occur, if you touch the edges due to their sharpness.

The centrifuge has one filter mat to prevent dust from entering the centrifuge. To clean the centrifuge proceed as follows: 1. Remove the 4 screws of the grid  on the right side of the centrifuge. 2. Remove the grid  and the filter mat. 3. Use a vacuum cleaner on both sides of the filter mat. 4. Insert the filter mat again. 5. Reinstall the grid.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care

4. Disinfection

WARNING

Do not touch infected parts. Hazardous infection is possible when touching the contaminated rotor and centrifuge parts. Infectious material can get into the centrifuge when a tube breaks or as a result of spills. In case of contamination, make sure that no one is put at risk. Disinfect the affected parts immediately.

CAUTION

Equipment can be damaged by inappropriate disinfection methods or agents. Make sure that the disinfection agent or the method will not damage the equipment. In doubt contact the manufacturer of the disinfection agent. Observe the safety precautions and handling instructions for the disinfection agents used.

After disinfection: 1. Rinse the centrifuge and all affected components with water. 2. Allow to fully drain and dry. 3. After disinfecting, treat the entire surface of aluminum parts including the cavities with corrosion protection oil
(70009824). Treat the bolts of the swing out rotor with bolt grease (75003786).

5. Decontamination

WARNING

Do not touch contaminated parts. Exposure to radiation is possible when touching the contaminated rotor and centrifuge parts. Radioactive material can get into the centrifuge when a tube breaks or as a result of spills. In case of contamination, make sure that no one is put at risk. Decontaminate the affected parts immediately.

CAUTION

Equipment can be damaged by inappropriate decontamination methods or agents. Make sure that the decontamination agent or the method will not damage the equipment. In doubt contact the manufacturer of the decontamination agent. Observe the safety precautions and handling instructions for the decontamination agents used.

After decontamination: 1. Rinse the centrifuge and all affected components with water. 2. Allow to fully drain and dry. 3. After decontaminating, treat the entire surface of aluminum parts including the cavities with corrosion protection oil
(70009824). Treat the bolts of the swing out rotor with bolt grease (75003786).

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care
6. Autoclaving
CAUT I ON Never exceed the permitted temperature and duration when autoclaving.

NOTICE

No chemical additives are permitted in the steam.

Always disassemble all parts before autoclaving, e.g. lids need to be removed before autoclaving bottles, buckets or rotors. Within the list of components marked parts can be autoclaved at 121 °C for 20 min. [à2 A­4] Make sure that the necessary sterility is achieved according to your requirements. After autoclaving, treat the entire surface of aluminum parts including the cavities with corrosion protection oil (70009824). Treat the trunnion bolts of the swing out rotor with bolt grease (75003786).

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care
7. Maintenance 7. 1. Preventive Maintenance
In order to keep this product able to perform the intended application in a reliable and safe state, ongoing preventive maintenance is necessary in accordance with the following recommended schedule:  For rotors and buckets mind the information in the Rotor and Components chapter. [à2 IV­2]  The anti-vibration mounts need to be replaced every 3 years.  The centrifuge needs to be decommissioned after 15 years or 150 000 cycles, whichever comes first. CAUTION Usage beyond these limits might affect the safety of the overall system.
7. 2. Service
The anti vibration mounts (50151096) need to be replaced every three years by a Thermo Fisher Scientific authorized service technician or earlier if noticed. If the anti vibration mounts are not replaced within this period the performance of the centrifuge can decrease. NOTICE In the worst case the centrifuge, the used components and the samples can be damaged. Thermo Fisher Scientific recommends having the centrifuge and components serviced once a year by an authorized service technician. The service technician checks the following:  electrical equipment;  suitability of set-up site;  centrifuge door lock and safety system;  rotor;  fixation of rotor and drive shaft of the centrifuge;  protective casing;  anti-vibration mounts. Before service, centrifuge and rotors should be thoroughly cleaned and decontaminated to ensure full and safe inspection can be completed. Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are performed for free during the warranty period and afterwards for a charge. That is only valid, if the centrifuge has only been maintained by an authorized Thermo Fisher Scientific service technician.
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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Maintenance and Care

8. Shipping and Disposal

WARNING

When removing the centrifuge and components from use for disposal you have to clean and if necessary, disinfect or decontaminate the entire system. In doubt contact the Thermo Fisher Scientific customer service.

For the disposal of the centrifuge mind the regulations in your country. Contact the Thermo Fisher Scientific Customer Service for the disposal of the centrifuge. For contact information check the back page of this manual or visit www.thermofisher.com/centrifuge For the countries of the European Union the disposal is regulated by the European Union's Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. Mind the information on transport and shipping. [à2 I­2] [à2 I­6]

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Troubleshooting
V

Troubleshooting

1. Mechanical Emergency Door Release

WARNING

Serious injuries can occur, if you touch a spinning rotor with your hands or tools. A rotor can still be spinning after a power failure occurs. Do not open the centrifuge before the rotor has stopped spinning. Do not touch a spinning rotor. Never use your hands or tools to stop a spinning rotor.

During a power failure, you will not be able to open the centrifuge door with the regular electric centrifuge door release. A mechanical override is provided to allow sample recovery in the case of an emergency. The mechanical override should be used only in emergencies and after the rotor has come to a complete stop. Always wait until the rotor has come to a stop without braking . The brake does not work without power supply. The deceleration process lasts much longer than usual. Proceed as follows: 1. Wait until the rotor has stopped . It can take longer than 40 minutes.
Use the sight glass in the centrifuge door to make sure that the rotor has stopped. The handle of the windshield lid has a marking, that shows a semicircle, if the rotor has stopped.

Figure V­1: Marking on windshield lid V-1

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Troubleshooting
2. Pull out the power supply plug. 3. On both sides of the housing are two white plastic plugs. Get these two white plastic plugs out of both sides of the
housing. Pull the release cords on both sides to trigger the mechanical centrifuge door release. The centrifuge door needs to be lifted manually. You need some force to lift the centrifuge door manually.
Figure V­2: Position of the mechanical emergency door release 4. Put the release cords back into the centrifuge and put the white plastic plugs back into the centrifuge housing. 5. Reconnect the centrifuge to the power supply when the power failure has ended.
2. Ice Formation
Warm humid air in combination with a cold centrifugation chamber can lead to formation of ice. To remove the ice out of the centrifugation chamber: 1. Open the centrifuge door. 2. Remove the rotor. [à2 III­14] 3. Let the ice melt.
NOTICE Do not use any sharp tools, aggressive liquids or fire to fasten the melting process. If necessary use warm water to fasten the melting process. 4. Remove the water from the centrifugation chamber. You can use the drain box (75007730) to remove the water from the centrifugation chamber. [à2 III­13] 5. Clean the centrifuge chamber. [à2 IV­1]
V-2

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Troubleshooting
3. Troubleshooting by Guide
CAUT I ON If problems occur other than those listed in this table, service technician must be contacted.
If an error occurs, follow the instructions on the touchscreen. Error messages have a red colored background.

Figure V­3: Example for an error message

Error Message

Description

E-1 to E-97

E-98

Centrifuge can not be operated.

Centrifuge run can not be started or centrifuge is braking.

E-99 Table V­1: Error messages

Troubleshooting Switch the centrifuge off and on again.
Make sure that the correct windshield lid is inserted into the holder.
Move the drive shaft slightly towards the front of the centrifuge.
If the error message still shows, contact a service technician. Imbalance detected.
Check the loading of the rotor.
Check the lubrication of the trunnion bolts of the rotor body.
Switch the centrifuge off and on again.
If the error message still shows, contact a service technician. Switch the centrifuge off and on again.
If the error message still shows, contact a service technician.

V-3

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Troubleshooting
4. Information for the Customer Service
If you need to contact a service technician, please provide the order no. and the serial no. of your centrifuge. This information can be found on the back near the inlet for the power supply cable. To identify the software version, proceed as follows: 1. Switch on the centrifuge. 2. Open the configuration menu. 3. Select Configuration. 4. Select Device.
Now you can read all required data.
V-4

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Technical Specifications
A

Technical Specifications

Environmental Conditions
Environmental Conditions during Storage and Shipping Permissible Ambient Temperature during Operation 200/208, 50/60 Hz 220/230/240 V, 50/60 Hz; 380/400/415 V, 50 Hz

Altitudes of up to 3 000 m above sea level Max. relative humidity 80 % up to 31 °C; decreasing linearly to 50 % relative humidity at 40 °C Temperature: -10 °C to 55 °C Humidity: 15 % to 85 %
2 °C to 25 °C 2 °C to 30 °C

Average Heat Dissipation Overvoltage Category Pollution Degree IP Running Time Maximum Speed nmax (Cryofuge 8 / Cryofuge 16) Minimum Speed nmin Maximum RCF Value at nmax (Cryofuge 8 / Cryofuge 16) Noise Level at Maximum Speed¹ Maximum Kinetic Energy Temperature Setting Range³

< 2 KW/h II 2 20 99 h 59 min 59 sec (1 second increment)
5 000 rpm / 4 700 rpm 300 rpm
7 295 x g / 7 187 x g 62 dB (A) 225 KJ -20 °C to 40 °C

Dimensions

Height (open door / closed door)

1 750 mm / 1 015 mm (68.9 in / 39.4 in)

Width

816 mm (32.1 in)

Depth (with mains connection)

990 mm (38.9 in)

Weight²

200, 208, 220, 230, 240 V, 50/60 Hz 475 kg (1 047 lb)

380, 400, 415 V, 50 Hz

495 kg (1 090 lb)

1 1 m in front of the instrument at 1.6 m height. 2 Without rotor. ³ ³ Applications using temperatures below 25 °C must pre-cool the centrifugation chamber and rotor.
Table A­1: Technical Data Thermo Scientific Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty

A­1

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Technical Specifications

1. Directives, Standards and Guidelines

Centrifuge
Thermo Scientific Cryofuge 8
Thermo Scientific Cryofuge 16
Thermo Scientific Cryofuge 16 Heavy Duty

Region
Europe
Single Phase 220, 230 V, 50 / 60 Hz
Poly Phase 380, 400, 415 V, 50 Hz

USA & Canada
Single Phase 208, 230, 240 V, 60 Hz

Directive
93/42/EEC Medical Devices
98/79/EC In Vitro Diagnostics or (EU) 2017/746*
2006/42/EC Machinery (Protective Goals)
2014/35/EU Low Voltage (Protective Goals)
2014/30/EU Electromagnetic Compatibility (EMC) (Protective Goals)
2011/65/EU RoHS Directive on the Restriction of the use of certain Hazardous Substances in electrical and electronic equipment
FDA listed
Product Code KSO
Centrifuge, Blood-Bank For In Vitro Diagnostic Use
Device Class 1

Japan
Single Phase 200 V, 50 / 60 Hz

China

CFDA listed

Single Phase 220 V, 50 Hz

Poly Phase 380 V, 50 Hz

*Please see Declaration of Compliance which legislation was applied.

Standard EN 61010-1 IEC 61010-2-020 EN 61010-2-101 EN 61326-1 Class B EN 62304 EN 61326-2-6 EN 62366-1 EN ISO 14971 EN ISO 13485
ANSI/UL 61010-1 IEC 61010-2-020 IEC 61010-2-101 IEC 61326-1 Class B EN 62304 EN 62366-1 EN ISO 14971 EN ISO 13485 FCC Part 15 IEC 61010-1 IEC 61010-2-020 IEC 61010-2-101 IEC 61326-1 Class B EN 62304 EN 62366-1 EN ISO 14971 EN ISO 13485

Table A­2: Directives, Standards and Guidelines Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty NOTE This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:  Reorient or relocate the receiving antenna.  Increase the separation between the equipment and receiver.  Connect the equipment into an outlet on a circuit different from that to which the receiver is connected.

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Technical Specifications

2. Refrigerants

Article No . 75007671

Centrifuge Thermo Scientific Cryofuge 8

Refrigerant Quantity

R-449A

1.80 kg

75007672 75007673 75007674 75007678

Thermo Scientific Cryofuge 8 Thermo Scientific Cryofuge 16 Thermo Scientific Cryofuge 16 Thermo Scientific Cryofuge 16 Heavy Duty

R-449A R-449A R-449A R-449A

1.80 kg 1.80 kg 1.80 kg 1.80 kg

Table A­3: Refrigerants used in the Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty

Contains fluorinated greenhouse gases in a hermetically sealed system.

Pressure 34 bar 34 bar 34 bar 34 bar 34 bar

GWP 1 397 1 397 1 397 1 397 1 397

CO2e 2.51 t
2.51 t 2.51 t 2.51 t 2.51 t

3. Mains Supply

The following data is to be taken into consideration, when selecting the mains connection socket.

Mains Voltage (V)

Frequency (Hz)

Rated Current (A)

Power Consumption (W)

Building Fuse (A)1

200

50

208

50

220

50

230

50

240

50

380

50

400

50

415

50

200

60

208

60

220

60

230

60

240

60

380

60

400

60

415

60

27.0

5 100

30

24.0

5 100

30

24.0

5 100

302

23.5

5 100

302

22.5

5 100

302

15.0

5 100

163

14.5

5 100

163

14.0

5 100

163

27.0

5 350

304

24.0

5 350

304

24.0

5 350

302,3

23.5

5 350

302,3

22.5

5 350

302,3

15.0

5 350

163

14.5

5 350

163

14.0

5 350

163

Equipment Fuse (A)
30 30 30 30 30 16 16 16 30 30 30 30 30 16 16 16

1 For the 200, 208, 220, 230, 240 V units the inrush current is 120 A for up to 1 second during the refrigeration start period. For the 380, 400, 415 V units it is 60 A. Circuit breakers, whether thermal or magnetic actuated, must have a delay type applicable for the start of motors. ² Use a 32 A Trip Char. C circuit breaker (D or K are also applicable). ³ For 380, 400, 415 V, 3-phase (unbalanced load, no neutral used) use a 16 A Trip Char. C circuit breaker (D or K are also applicable). 4 For North America: use for example GES-9888 30 A.

Table A­4: Mains Supply

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Technical Specifications

MEDICAL

4. Components

Article No . Description

Rotor Capacity

75007742 75007740 75007741 75007730

Centri-Log Plus Sample Tracking Kit Networking Access Kit Drain Box (600 x 400 x 50 mm)

HAEMAFlex 6 Swinging Bucket

75003861 Rotor

6 x 500 ml

75003881 75003834
75003837 75003838 75003839 75003841 75003833 75003835 75003842
75003859 75003836
75003857 75003858

HAEMAFlex 8 Swinging Bucket Rotor
Oval Single Blood Bag Bucket (set of 2)
450 ­ 550 ml Quad or Quint Blood Bag Adapter (set of 2)
400 ­ 450 ml Double or Triple Blood Bag Adapter (set of 2)
250 ml Platelet-rich Plasma or Buffy Coat Bag Adapter (set of 2)
400 ­ 450 ml Single or Double Blood Bag Adapter (set of 2)
Linerstand for Single Blood Bag Adapters (set of 2)
Single Blood Bag Bucket with Filter Pack (set of 2)
450 ­ 550 Triple, Quad or Quint Blood Bag with Additive Solution Adapter (set of 2)
Filter Holder
Single Blood Bag Round Bucket (set of 2)
450 ­ 500 ml Triple or Quad Blood Bag Adapter (set of 2)
450 ml Single or Double Blood Bag Adapter (set of 2)

8 x 550 ml 6 or 8 x 550 ml 6 or 8 x 450 ml 6 or 8 x 250 ml 6 or 8 x 450 ml
6 or 8 x 550 ml
6 or 8 x 500 ml 6 or 8 x 450 ml

HAEMAFlex 12 Swinging Bucket

75003862 Rotor

12 x 500 ml

75003882 75003846
75003899
75003851
75003852
75003853

HAEMAFlex 16 Swinging Bucket

Rotor

16 x 500 ml

Double Blood Bag Bucket (set of 2)

Double Quint Blood Bag Adapter for 12 or 16 x 500 ml XXL size, HD (set of 2; 110 x 88 mm)

Double Quint Blood Bag Adapter for XXL size (set of 2; 110 x 88 mm)

12 or 16 x 500 ml

Double Quint Blood Bag Adapter for XL size (set of 2; 110 x 76 mm)

12 or 16 x 500 ml

Double Quint Blood Bag Adapter for M 12 or 16 x 450 ml size (set of 2; 110 x 57 mm)

Max . Speed

Max . RCF

Autoclavable (121 °C, 20 min;
 = no,  = yes)









5 000 rpm 7 127 x g













5 000 rpm 7 211 x g







5 000 rpm 7 295 x g











4 700 rpm 7 187 x g











A­4

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Technical Specifications

IN VITRO

Article No . Description

75003855 75003868

Hook Adapter for Cord Blood Separations, for use with M size Adapters
Hook Adapter for Cord Blood Separations, for use with XXL size Adapters

75003866 Dummy Weights

75003832 Linerstand for Double Blood Bag Adapters (set of 2)

75003964 Oval Bucket (set of 2)

75003341 Adapter 52 x 5/7 ml (set of 2)

75003342 Adapter 42 x 10 ml (set of 2)

75003343 Adapter 26 x 15 ml (set of 2)

75003344 Adapter 11 x 50 ml (set of 2)

75003345 Adapter MP DW (set of 2)

Other components for HAEMAFlex rotors

75003843 Blood Bag Spacer (set of 12)

75006681

Rubber Volume Compensation Plates (set of 12)

75005759 Rubber Balancing Plates (set of 4)

Table A­5: Components

Rotor Capacity 12 or 16 x 300 ml

Max . Speed

12 or 16 x 300 ml

312 or 416 x 5/7 mL 252 or 336 x 10 mL 156 or 208 x 15 mL
66 or 88 x 50 mL 30 MP / 12 DW or 40 MP / 16 DW

Autoclavable Max . RCF (121 °C, 20 min;
 = no,  = yes)

1 328 x g



1 328 x g



 
     

 


A­5

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
B

Rotors

Items Supplied for Rotors

Article No .
75003861 75003881 75003862 75003882

Item Rotor (incl . Windshield Bowl and Windshield Lid) Thermo Scientific HAEMAFlex 6 Thermo Scientific HAEMAFlex 8 Thermo Scientific HAEMAFlex 12 Thermo Scientific HAEMAFlex 16

20360104 75003786 70009824 66309 75009709

Rotor Locking Tool Bolt Grease Corrosion Protection Oil Cleaning Pad (6x) Information Card

Table B­1: Items supplied for rotors

Quantity 1
1 1 1 1 1

B­1

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 6 Rotor
with Single Blood Bag Buckets (oval)

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed nmax Maximum RCF-Value at n
max
K-Factor at n max
Radius max. / min. Angle

200, 208, 220, 230, 240 V 6 x 1000 ml 5 000 rpm 7 127 x g 8 519
255 mm / 110 mm 90°

Cryofuge 8

380, 400, 415 V 6 x 1000 ml 5 000 rpm 7 127 x g 8 519
255 mm / 110 mm 90°

Maximum Speed at 4 °C

5 000 rpm

5 000 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)
Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 7127 x g / 5000 rpm 5000 x g / 4188 rpm 4000 x g / 3746 rpm 2500 x g / 2961 rpm
Weight
Rotor Body (empty) with Windshield Bowl Windshield Lid

50 Hz: 1 °C 60 Hz: -2 °C
No

50 000
18 500 35 000 53 000 124 000 19.8 kg 18.3 kg
1.5 kg

50 Hz: 1 °C 60 Hz: -2 °C
No

1 Cycle numbers stated are only valid, if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.
Table B­2: HAEMAFlex 6 Rotor with Single Blood Bag Buckets (oval)

B­2

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 6 Rotor
with Single Blood Bag Buckets (round)

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed nmax Maximum RCF-Value at n
max
K-Factor at n max
Radius max. / min. Angle

200, 208, 220, 230, 240 V 6 x 1000 ml 5 000 rpm 7 295 x g 8 216
261 mm / 116 mm 90°

Cryofuge 8

380, 400, 415 V 6 x 1000 ml 5 000 rpm 7 295 x g 8 216
261 mm / 116 mm 90°

Maximum Speed at 4 °C

5 000 rpm

5 000 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)
Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 7295 x g / 5000 rpm 5000 x g / 4140 rpm 4000 x g / 3703 rpm 2500 x g / 2927 rpm
Weight
Rotor Body (empty) with Windshield Bowl Windshield Lid

50 Hz: 1 °C 60 Hz: -2 °C
No

50 000
14 000 31 000 50 000 138 000 19.8 kg 18.3 kg
1.5 kg

50 Hz: 1 °C 60 Hz: -2 °C
No

1 Cycle numbers stated are only valid, if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.
Table B­3: HAEMAFlex 6 Rotor with Single Blood Bag Buckets (round)

B­3

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 6 Rotor
with Single Blood Bag Buckets with Filter Pack

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed nmax Maximum RCF-Value at n
max
K-Factor at n max
Radius max. / min. Angle

200, 208, 220, 230, 240 V 6 x 1000 ml 5 000 rpm 7 211 x g 8 365
258 mm / 113 mm 90°

Cryofuge 8

380, 400, 415 V 6 x 1000 ml 5 000 rpm 7 211 x g 8 365
258 mm / 113 mm 90°

Maximum Speed at 4 °C

5 000 rpm

5 000 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)
Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 7211 x g / 5000 rpm 5000 x g / 4164 rpm 4000 x g / 3724 rpm 2500 x g / 2944 rpm
Weight
Rotor Body (empty) with Windshield Bowl Windshield Lid

50 Hz: 1 °C 60 Hz: -2 °C
No

50 000
14 000 31 000 50 000 138 000 19.8 kg 18.3 kg
1.5 kg

50 Hz: 1 °C 60 Hz: -2 °C
No

1 Cycle numbers stated are only valid, if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.
Table B­4: HAEMAFlex 6 Rotor with Single Blood Bag Buckets with Filter Pack

B­4

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 8 Rotor
with Single Blood Bag Buckets (oval)

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed nmax Maximum RCF-Value at n
max
K-Factor at n max
Radius max. / min. Angle

200, 208, 220, 230, 240 V 8 x 1000 ml 4 600 rpm 7 026 x g 8 019
297 mm / 152 mm 90°

Cryofuge 8

380, 400, 415 V 8 x 1000 ml 4 600 rpm 7 026 x g 8 019
297 mm / 152 mm 90°

Maximum Speed at 4 °C

4 600 rpm

4 600 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)

50 Hz: 4 °C 60 Hz: 2 °C

50 Hz: 4 °C 60 Hz: 2 °C

Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 7026 x g / 4600 rpm 5000 x g / 3881 rpm 4000 x g / 3471 rpm 2500 x g / 2744 rpm
Weight

No

No

50 000

14 000 31 000 50 000 138 000
28.8 kg

Rotor Body (empty) Windshield Lid Windshield Bowl

21.9 kg 1.9 kg 5.0 kg

1 Cycle numbers stated are only valid, if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.

Table B­5: HAEMAFlex 8 Rotor with Single Blood Bag Buckets (oval)

B­5

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 8 Rotor
with Single Blood Bag Buckets (round)

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed nmax Maximum RCF-Value at n
max
K-Factor at n max
Radius max. / min. Angle

200, 208, 220, 230, 240 V 8 x 1000 ml 4 600 rpm 7 144 x g 7 831
302 mm / 157 mm 90°

Cryofuge 8

380, 400, 415 V 8 x 1000 ml 4 600 rpm 7 144 x g 7 831
302 mm / 157 mm 90°

Maximum Speed at 4 °C

4 600 rpm

4 600 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)

50 Hz: 4 °C 60 Hz: 2 °C

50 Hz: 4 °C 60 Hz: 2 °C

Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 7144 x g / 4600 rpm 5000 x g / 3848 rpm 4000 x g / 3442 rpm 2500 x g / 2721 rpm
Weight

No

No

50 000

14 500 31 000 50 000 138 000
28.8 kg

Rotor Body (empty) Windshield Lid Windshield Bowl

21.9 kg 1.9 kg 5.0 kg

1 Cycle numbers stated are only valid, if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.

Table B­6: HAEMAFlex 8 Rotor with Single Blood Bag Buckets (round)

B­6

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 8 Rotor
with Single Blood Bag Buckets with Filter Pack

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed nmax Maximum RCF-Value at nmax K-Factor at nmax Radius max. / min. Angle

200, 208, 220, 230, 240 V 8 x 1000 ml 4 600 rpm 7 073 x g 7 942
299 mm / 154 mm 90°

Cryofuge 8

380, 400, 415 V 8 x 1000 ml 4 600 rpm 7 073 x g 7 942
299 mm / 154 mm 90°

Maximum Speed at 4 °C

4 600 rpm

4 600 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)

50 Hz: 4 °C 60 Hz: 2 °C

50 Hz: 4 °C 60 Hz: 2 °C

Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 7073 x g / 4600 rpm 5000 x g / 3868 rpm 4000 x g / 3459 rpm 2500 x g / 2735 rpm
Weight

No

No

50 000

14 000 31 000 50 000 138 000
28.8 kg

Rotor Body (empty) Windshield Lid Windshield Bowl

21.9 kg 1.9 kg 5.0 kg

1 Cycle numbers stated are only valid, if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.

Table B­7: HAEMAFlex 8 Rotor with Single Blood Bag Buckets with Filter Pack

B­7

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 12 Rotor
with Double Blood Bag Buckets

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed nmax Maximum RCF-Value at n
max
K-Factor at n max
Radius max. / min. Angle

Cryofuge 16 / Cryofuge 16 Heavy Duty

200, 208, 220, 230, 240 V

380, 400, 415 V

12 x 1000 ml

12 x 1000 ml

4 700 rpm

4 700 rpm

7 187 x g

7 187 x g

7 909

7 909

291 mm / 146 mm

291 mm / 146 mm

90°

90°

Maximum Speed at 4 °C

4 600 rpm

4 600 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)

50 Hz: 5 °C 60 Hz: 2 °C

50 Hz: 5 °C 60 Hz: 2 °C

Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 7187 x g / 4700 rpm 5000 x g / 3921 rpm 4000 x g / 3507 rpm 2500 x g / 2772 rpm
Weight

No

No

50 000

14 000 31 000 50 000 137 000
25.6 kg

Rotor Body (empty) Windshield Lid Windshield Bowl

18.5 kg 1.7 kg 5.4 kg

1 Cycle numbers stated are only valid, if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.

Table B­8: HAEMAFlex 12 Rotor with Double Blood Bag Buckets

B­8

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 12 Rotor
with Oval Buckets

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed n
max
Maximum RCF-Value at n max
K-Factor at nmax Radius max. / min. Angle

Cryofuge 16 / Cryofuge 16 Heavy Duty

200, 208, 220, 230, 240 V

380, 400, 415 V

6 x 2000 ml

6 x 2000 ml

4 700 rpm

4 700 rpm

7 187 x g

7 187 x g

7 909

7 909

291 mm / 146 mm

291 mm / 146 mm

90°

90°

Maximum Speed at 4 °C

4 600 rpm

4 600 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)

50 Hz: 5 °C 60 Hz: 2 °C

50 Hz: 5 °C 60 Hz: 2 °C

Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 7187 x g / 4700 rpm 5000 x g / 3921 rpm 4000 x g / 3507 rpm 2500 x g / 2772 rpm
Weight

No

No

50 000

16 000 35000 56 000 152 000
25.6 kg

Rotor Body (empty) Windshield Lid Windshield Bowl

18.5 kg 1.7 kg 5.4 kg

1 Cycle numbers stated are only valid if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.

Table B­9: HAEMAFlex 12 Rotor with Oval Buckets

B­9

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors

HAEMAFlex 16 Rotor
with Double Blood Bag Buckets

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed n
max
Maximum RCF-Value at nmax K-Factor at nmax Radius max. / min. Angle

Cryofuge 16 / Cryofuge 16 Heavy Duty

200, 208, 220, 230, 240 V

380, 400, 415 V

16 x 1000 ml

16 x 1000 ml

3 900 rpm

3 900 rpm

5 374 x g

5 374 x g

10 227

10 227

316 mm / 171 mm

316 mm / 171 mm

90°

90°

Maximum Speed at 4 °C

3 900 rpm

3 900 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)
Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 5374 x g / 3900 rpm 5000 x g / 3762 rpm 4000 x g / 3365 rpm 2500 x g / 2660 rpm
Weight

50 Hz: 0 °C 60 Hz: -2 °C
No

50 000
26 500 31 000 50 000 137 000 32.1 kg

50 Hz: 0 °C 60 Hz: -2 °C
No

Rotor Body (empty) Windshield Lid Windshield Bowl

24.7 kg 1.9 kg 5.5 kg

1 Cycle numbers stated are only valid, if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.

Table B­10: HAEMAFlex 16 Rotor with Double Blood Bag Buckets

B­10

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Rotors
HAEMAFlex 16 Rotor
with Oval Buckets

Technical Data
Centrifuge Voltage Rotor Capacity Maximum Speed n
max
Maximum RCF-Value at n max
K-Factor at nmax Radius max. / min. Angle

Cryofuge 16 / Cryofuge 16 Heavy Duty

200, 208, 220, 230, 240 V

380, 400, 415 V

8 x 2000 ml

8 x 2000 ml

3 900 rpm

3 900 rpm

5 374 x g

5 374 x g

10 227

10 227

316 mm / 171 mm

316 mm / 171 mm

90°

90°

Maximum Speed at 4 °C

3 900 rpm

3 900 rpm

Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)

50 Hz: 0 °C 60 Hz: -2 °C

50 Hz: 0 °C 60 Hz: -2 °C

Aerosol-tight
Rotor Body Max. Cycle Number
Buckets Max. Cycle Number1 at 5374 x g / 3900 rpm 5000 x g / 3762 rpm 4000 x g / 3365 rpm 2500 x g / 2660 rpm
Weight

No

No

50 000

30 000 35 000 56 000 152 000
32.1 kg

Rotor Body (empty) Windshield Lid Windshield Bowl

24.7 kg 1.9 kg 5.5 kg

1 Cycle numbers stated are only valid if the according speed (or rcf) is always set for centrifugation. If different speed (or rcf) settings are used, the smallest cycle number stated is valid.

Table B­11: HAEMAFlex 16 Rotor with Oval Buckets

B­11

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­1

CHEMICAL 2-MERCAPTOETHANOL ACETALDEHYDE ACETONE ACETONITRILE ALCONOXTM ALLYL ALCOHOL ALUMINUM CHLORIDE FORMIC ACID (100%) AMMONIUM ACETATE AMMONIUM CARBONATE AMMONIUM HYDROXIDE (10%) AMMONIUM HYDROXIDE (28%) AMMONIUM HYDROXIDE (CONC.) AMMONIUM PHOSPHATE AMMONIUM SULFATE
S M U /

SSU/

S MS /

SUSSUSS/

SSSS

S/

UU/

/

/

M/

U/

/

/

MU U U MM/

MS U U S U MS S U U S U S U U U S S U

SSU/

S MS /

S S U S U MU U /

S MU

UUS/

SSS/

S S S S S S MS S S S S

/

/

/

U/

/

S/

/

/

/

S/

S S MS S S /

U U S S S S U S S S S MS S S S / S S S

/

S MU /

/

U/

/

/

/

U/

S MU U S S /

SSU/

SSS/

SSSSSSSU/

SSS

MS U S S S S S S S S S S S U U / S S S

U U S U S S MS S S S S / S U MS S S S

U U S U S U MS S S S S U S U MS S S S

U U U U S U MS / S / S U S U U S S S /

U/

S/

SSSSSSSS/

S S M/

SSS

U MS /

SSUSSSSSSSSS/

SSS

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

US MS US US SS MS SS US SS SS SS SS MS SS SS

SSS

U/

S

MMS

SSS

SSS

/

/

S

MU U

/

US

SSS

S MS

SSS

SSS

SSS

S MS

SUS

SS /U UU UU SU // SS /U SS SS MS MS /U SS SU

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­2

CHEMICAL AMYL ALCOHOL ANILINE SODIUM HYDROXIDE (<1%) SODIUM HYDROXIDE (10%) BARIUM SALTS BENZENE BENZYL ALCOHOL BORIC ACID CESIUM ACETATE CESIUM BROMIDE CESIUM CHLORIDE CESIUM FORMATE CESIUM IODIDE CESIUM SULFATE CHLOROFORM
S M U /

S/

MU /

/

SS/

M/

S/

MS S S S M/

S S U U S U S MS U U U U U U U / S MU

U/

MS S S /

/

S MS S /

S MMS S S S

U/

MU /

/

U/

MMS S U S U U S S S S

MU S /

S S S S S S S S S S S M/

SSS

S S U U S U MU S U U S U U U MU MU U

S/

UU/

/

MM/

M/

SUUUUUUU/

U S S MS S U S S S S S S S S S U S S S

M/

S/

SSS/

SSSS/

SS/

/

SSS

MS S /

SSS/

SSSSSSS/

/

SSS

MS S U S S S / S S S S S S S / / S S S

MS S /

SSS/

SSSSSSS/

/

SSS

MS S /

SSS/

SSSSSSS/

/

SSS

MS S /

SSS/

SSSSSSS/

/

SSS

U U U U S S MU S U U MU MU U U MMU

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

/

/

US

SS

SS

SS

US

MS

SS

SS

SS

SS

SS

SS

SS

US

U/

S

SSS

MS S

MS S

S MS

UUS

M/

S

SSS

S MS

S MS

S MS

S MS

S MS

S MS

UUU

/M US /U /U SS US /S SS SS SS SS SS SS SS MS

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­3

CHEMICAL CHROMIC ACID (10%) CHROMIC ACID (50%) CRESOL MIXTURE CYCLOHEXANE DEOXYCHOLATE DISTILLED WATER DEXTRAN DIETHYL ETHER DIETHYL KETONE DIETHYLPYRO-CARBONATE DIMETHYLSULFOXIDE DIOXANE FERRIC CHLORIDE ACETIC ACID (GLACIAL) ACETIC ACID (5%)
S M U /

U/

UUSUU/

S S S U S S MU MS S U MS

U/

UU/

UU/

/

/

S U U S MU MS S U MS

SSU/

/

/

S/

SUUUUUU/

/

UU/

US

SSS/

S S S U S U S S U U U MS MU MMS

SSS/

SSS/

SSSSSSS/

/

SSSSS

SSSSSSSSSSSSSSSSSSSSSS

MS S S S S S / S S S S S S S S S S S S S S

SSUUSSSUSUUSUUUUUUUUUS

S/

UU/

/

M/

SU/

S/

MU U U MM/

US

SSU/

SSS/

SSUSUSU/

/

S S S MS

SSUUSSS/ SUSSUSUU/ SSUUS

MS U U S S MMS U U S U MU U / MMMU S

UUS/

/

/

MS /

M/

S/

S/

/

/

SS/

/

/

S S U U S S U MS U S U U U U U MS U MU S

S S MS S S MS S S S S MS S S S S S S MS

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

MU S S S

/

U M/

S

SSSUS

U MMU S

SSSSS

SSSSS

S MS S S

S S S MU

/

/

SUU

SSSSS

SSSUU

SSSUU

MU S / S

UUS/ U

S MS S M

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­4

CHEMICAL ACETIC ACID (60%) ETHYL ACETATE ETHYL ALCOHOL (50%) ETHYL ALCOHOL (95%) ETHYLENE DICHLORIDE ETHYLENE GLYCOL ETHYLENE OXIDE VAPOR FICOLL-HYPAQUETM HYDROFLUORIC ACID (10%) HYDROFLUORIC ACID (50%) HYDROCHLORIC ACID (CONC.) FORMALDEHYDE (40%) GLUTARALDEHYDE GLYCEROL GUANIDINE HYDROCHLORIDE
S M U /

S S U U S S U / S MS U U MU S MS

MMU U S S MMS S U S U MU U / S

S S S S S S MS S S S S U S U S S S

S S S U S S MS S S S S U S U / S S

S/

UU/

/

S M/

UUSUUUUUU

SSSSSSSSSSSS/ SUSSS

S/

U/

/

U/

/

SU/

S/

S M/

/

S

MS S /

SSS/

SSSS/

SS/

SS

U U U M/

/

U/

/

UUS/

S MU S S

UUUU/

/

U/

/

UUUUSUUUS

UUUU/

U U M/

U MU U MU U U /

MMMS S S S MS S S S MS S S U S

SSSS/

/

S/

SSSSSSS/

/

S

MS S /

SSSSSSSSSSSS/

S

UUS/

SSS/

SSSSSSS/

/

S

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

MS MS

SUUS

SSSS

S MS S

U/

US

SSSS

SSUS

SSSS

S S MS

S MMS

S/

US

S MS S

SS/

/

SSSS

SSSS

MU S

MMS

S MS

SUS

U/

S

S MS

USS

S MS

UUU

UUU

UUU

S MS

SSS

SSS

SUS

MU UU MU MU /S MS SU SS // /M // MU // SS SS

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­5

CHEMICAL HAEMO-SOLTM HEXANE ISOBUTYL ALCOHOL ISOPROPYL ALCOHOL IODOACETIC ACID POTASSIUM BROMIDE POTASSIUM CARBONATE POTASSIUM CHLORIDE POTASSIUM HYDROXIDE (5%) POTASSIUM HYDROXIDE (CONC.) POTASSIUM PERMANGANATE CALCIUM CHLORIDE CALCIUM HYPOCHLORITE KEROSENE SODIUM CHLORIDE (10%)
S M U /

SSS/

/

/

S/

SSSSSSS/

/

SSSSSSSSSS

SSS/

SSS/

S S U S U MU S S U S S MS U S S U S

/

/

MU /

/

SS/

U/

S U S S MS S S /

SSS/

S/

S

MMMU S S S S S U S S U S U MS S S S S S S MMMS

S S M/

SSS/

S MS S MS S /

MS S S S S MS S MM

USS/

SSS/

SSSSSSSSSSS/

S S S MS S S

MU S S S S S / S S S S S S U S S S S S S S S S S S S

USS/

SSSSSSSSSSS/

SSSSSSSUSSS

U U S S S S M/ S S S S / S U S S S S S S S MU MS U

U U MU /

/

M/

MS S /

U MU U U S M/

MU /

UU/

U

SSS/

SSS/

S S S U S S S M/

S MS U S S MS U S

MU S S S S S S S S S S S S MS / S S S S S S MS S S

M/

U/

S MMS /

M/

S/

S MS /

S S S MS MU S /

S

SSS/

S S S U S MU S U MMS /

MMMS S U S S U S

S/

SSSSSS/

/

/

SSSSS/

SSSS/

S S M/

S

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­6

CHEMICAL SODIUM CHLORIDE (SAT'D) CARBON TETRACHLORIDE AQUA REGIA SOLUTION 555 (20%) MAGNESIUM CHLORIDE MERCAPTOACETIC ACID METHYL ALCOHOL METHYLENE CHLORIDE METHYL ETHYL KETONE METRIZAMIDETM LACTIC ACID (100%) LACTIC ACID (20%) N/BUTYL ALCOHOL N/BUTYL PHTHALATE N, N-DIMETHYLFORMAMIDE
S M U /

U/

SUSSS/

/

/

/

SSSSS/

SS/

S/

S S M/

S

U U MS S U MU S U U S U MU S S MMS MMMMU S S

U/

UU/

/

U/

/

/

/

/

UUUUUUU/

/

/

/

/

S/

M

SSS/

/

/

S/

SSSSSSS/

/

SSS/

SSSSSS

MS S /

S S S S S S S S S S S S S S S S S S S MS S S

USU/

S MS /

S MS U U U U /

S U U S MS U S S S S

S S S U S S MS S S S S U S U MS S S S S S S MS MU

U U U U MS S U S U U S U U U U U MU U U S S MU S U

S S U U S S MS S U U S U S U U U S S U U S S S S U U

MS S /

SSS/

SSSS/

SS/

/

S S S S S S MS S S

/

/

S/

/

/

/

/

/

MS U /

S S S MS S /

MS MS S /

S

/

/

SS/

/

/

/

/

MS M/

S S S S S S S MS MS S /

S

S/

SU/

/

S/

/

S M/

U S MS S S S MMS M/

S/

S

SSU/

SSS/

S U U S U U U M/

U U S U S MMS U S

S S S U S MS / S S U S U S U U / S S U U S MS S S U

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­7

CHEMICAL SODIUM BORATE SODIUM BROMIDE SODIUM CARBONATE (2%) SODIUM DODECYL SULFATE SODIUM HYPOCHLORITE (5%) SODIUM IODIDE SODIUM NITRATE SODIUM SULFATE SODIUM SULFIDE SODIUM SULFITE NICKEL SALTS OILS (PETROLEUM) OILS (OTHER) OLEIC ACID OXALIC ACID
S M U /

MS S S S S S S S S S U S S S S / S S S S S S MS S S

USS/

SSS/

SSSSSSSS/

S S S S S S MS S S

MU S S S S S S S S S S S S U S S S S S S S S S S S S

SSS/

SSS/

SSSSSSS/

SSSSSSSSSSS

U U MS S MU S S MS S S MS S S S MS S S MU S MS

MS S /

SSS/

SSSSSSS/

/

S S S S S S MS S S

SSS/

SSSSSSSSSSSS/

SSSSSUSSSS

USS/

S S S S S S S S S S S S S S S S S S S MS S S

S/

SS/

/

/

S/

/

/

SSSUU/

/

S/

/

/

S S M/

S

SSS/

S S S S MS S S S S S M/

SSSSSSSSSS

USSSSS/

SSS/

/

SSSS/

S S S S S S MS S S

SSS/

/

/

S U S S S S U U MS MU U S S S U S S S S

S/

S/

/

/

S MS S S S U S S S S U S S S S /

S S MS

S/

U S S S U U S U S S MS S S S S S S S S MU S MM

U U MS S S U S S S S S U S U S S S S S S S S U MS S

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­8

CHEMICAL PERCHLORIC ACID (10%) PERCHLORIC ACID (70%) PHENOL (5%) PHENOL (50%) PHOSPHORIC ACID (10%) PHOSPHORIC ACID (CONC.) PHYSIOLOGIC MEDIA (SERUM, URINE) PICRIC ACID PYRIDINE (50%) RUBIDIUM BROMIDE RUBIDIUM CHLORIDE SUCROSE SUCROSE, ALKALINE SULFOSALICYLIC ACID NITRIC ACID (10%)
S M U /

U/

U/

SUU/

S MM/

/

MU MS MM/

MS

UUU/

/

UU/

S U MU U MU U U MMU MS

USU/

S MM/

S U MU U S U MS MS U U S

USU/

S U M/

S U MU U U U U S U MU U S

U U MS S S U S S S S U / S S S S S S S S S

U U MM/

/

US/

MS U U MMS S S MS MS

MS S S /

/

S/

SSSSSSSSSSSSSS

SSU/

S MS S S MS U S S S U S S S S U S

U S U U S U U / U S S U U MU U / U S MU S

MS S /

SSS/

SSSSSSS/

/

SSSSS

MS S /

SSS/

SSSSSSS/

/

SSSSS

MS S /

SSSSSSSSSSSSSSSSSS

MS S /

SSS/

SSSSSSUSSSSSSS

UUSSSSS/

SSSUSSS/

SSS/

SS

USUSSUU/ SUSU/ SSSSSSSSS

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

U/

S/

S

UUSUS

U MMMS

U U U MS

U MU S S

U MU / S

SSSSS

U MS MS

SUUUU

S MS S S

S MS S S

SSSSS

S MS S S

SUSSS

MS S S S

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­9

CHEMICAL NITRIC ACID (50%) NITRIC ACID (95%) HYDROCHLORIC ACID (10%) HYDROCHLORIC ACID (50%) SULFURIC ACID (10%) SULFURIC ACID (50%) SULFURIC ACID (CONC.) STEARIC ACID TETRAHYDROFURAN TOLUENE TRICHLOROACETIC ACID TRICHLOROETHANE TRICHLOROETHYLENE TRISODIUM PHOSPHATE TRIS BUFFER (NEUTRAL PH)
S M U /

U S U MS U U / S U S U U MMU MMMS

U/

UU/

UU/

/

U U U U MU U U U MU

U U MS S S U / S S S U U S U S S S S S

U U U U S U U / S MS U U MU U S S S S

MU U S S U U / S S MU S S S S S S S S

MU U U S U U / S S MU U S U U MS S S

MU U U /

U U M/

/

MU U S U U U MS U

S/

S/

/

/

S MS S S S /

SSSSSSS

S S U U S U U MS U U S U U U / MU U U

S S U U S S MU S U U S U U U S U MU U

UUU/

S S U MS U S U U S M/

MS S U

S/

U/

/

/

MU /

U/

SUUUUUUUU

/

/

UU/

/

/

U/

U/

SUUUUUUUU

/

/

/

S/

/

M/

/

/

/

/

/

S/

/

SSS/

USSSSSS/ SSSSSSSSSSSS

SATISFACTORY MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE UNSATISFACTORY, NOT RECOMMENDED PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

SS

US

SS

MS

SS

SS

MS

SS

US

US

US

US

US

/

S

SS

U S S MS

USS/ S

S U MS S

MU U MM

UUUSS

U U U MS

UUU/ S

MMS S S

USSUU

USUUM

U U U MU

U/

S/

S

U/

U/

S

/

/

S/

S

SSSSS

Chemical Compatibility Chart

MATERIAL
ALLUMINUM ANODIC COATING FOR ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE CARBON FIBER/EPOXY DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVYNIL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL Titanium TYGONTM VITONTM

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­10

CHEMICAL TRITON X/100TM UREA HYDROGEN PEROXIDE (10%) HYDROGEN PEROXIDE (3%) XYLENE ZINC CHLORIDE ZINC SULFATE CITRIC ACID (10%)

SSS/

SSS/

SSSSSSSSSSSSSSSSSSS

S/

USSSS/

/

/

/

S S S MS S S S /

S S S MS /

S

U U MS S U U / S S S U S S S MU S S S S S S MS U S

S MS S S /

S/

S S S S S S S S MS S S S S S S S S S

S S U S S S MU S U U U U U U MU MU U U S U MS U S

UUSSSSUSSSSSSSSSSSSSSSSUSSS

USS/

SSSSSSSSSSSSSSSSSSSSSSS

MS S MS S MS S S S S S S S S MS S S S S S S S S S

S

SATISFACTORY

M

MODERATE ATTACK, MAY BE SATISFACTORY FOR USE IN CENTRIFUGE DEPENDING ON LENGTH OF EXPOSURE, SPEED INVOLVED, ETC.; SUGGEST TESTING UNDER ACTUAL CONDITIONS OF USE

U

UNSATISFACTORY, NOT RECOMMENDED

/

PERFORMANCE UNKNOWN; SUGGEST TESTING, USING SAMPLE TO AVOID LOSS OF VALUABLE MATERIAL

¹ Polyethlyeneterephtalate

Table C­1: Chemical Compatibility

NOTICE

Chemical resistance data is included only as a guide to product use. Because no organized chemical compatibility data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots.

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges Chemical Compatibility Chart
C­11

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges

Index
A
Acceleration / Deceleration Profiles III­9 Aerosol-tight Rotors III­9 Autoclaving IV­7
B
Basics IV­1 Before Installation III­2
C
Centrifugation III­12 Chemical Compatibility Chart C­1 Cleaning IV­3 Cleaning Intervals IV­1 Close the Centrifuge Door III­9 Components A­4 Control and Configuration II­3
D
Decontamination IV­6 Directives, Standards and Guidelines A­2 Disinfection IV­6 Drain Box III­13
E
Entering Parameters III­9 Explanation of RCF-Value III­10
F
Filter Mat IV­5
H
HAEMAFlex 6 Rotor with Single Blood Bag Buckets (round) B­3 HAEMAFlex 6 Rotor with Single Blood Bag Buckets with Filter Pack B­4 HAEMAFlex 8 Rotor with Single Blood Bag Buckets (oval) B­5 HAEMAFlex 8 Rotor with Single Blood Bag Buckets (round) B­6 HAEMAFlex 8 Rotor with Single Blood Bag Buckets with Filter Pack B­7 HAEMAFlex 12 Rotor with Double Blood Bag Buckets B­8 HAEMAFlex 12 Rotor with Oval Buckets B­9 HAEMAFlex 12 Rotor with Oval Buckets B­9 HAEMAFlex 16 Rotor with Double Blood Bag Buckets B­10 HAEMAFlex 16 Rotor with Oval Buckets B­11

I
Ice Formation V-2 Install a Rotor III­1 Intended Use vii Intended User vii Items Supplied vi Items Supplied for Rotors B­1
L
Location I­1
M
Mains Connection I­5 Mains Supply A­3 Maintenance and Care IV­1 Maximum Loading III­8 Mechanical Emergency Door Release V-1 Metal Parts IV­2
O
Open the Centrifuge Door III­1 Operation III­1
P
Plastic Parts IV­2 Precautions viii Preface vi Preselect Speed / RCF-Value III­9 Preselect Temperature III­10 Preventive Maintenance IV­2 Pre-warm or Pre-cool the Centrifuge III­10 Programs III­11
R
Refrigerants A­3 Rotor and Components Inspection IV­2 Rotor Installation III­3 Rotors B­1 Run Parameters II­3 Runtime Preselection III­10
S
Setting Up I­3 Set Up Conditions viii Shipping I­6 Shipping and Disposal IV­9 Status II­2 Storage I­5 6 x 1000 mL Swinging Bucket Rotor with Round Buckets B­5 6 x 2000 mL Swinging Bucket Rotor with Oval Buckets B­9, B­10 Switch on the Centrifuge III­1 Symbols used in the Manual xiii Symbols used on the Centrifuge xi

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
T
Technical Specifications A­1 Transport and Set Up I­1 Transporting I­2 Troubleshooting V-1, V-3
U
Unpacking I­3 Use of Tubes and Consumables III­9

Thermo Scientific Cryofuge 8 / 16 Thermo Scientific Cryofuge 16 Heavy Duty

Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany

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50149611 is the original instruction manual.

en



References

• Cross Divisional Warranty
• World Health Organization (WHO)