User Guide for Oxford Nanopore models including: P2 Integrated, PRO-INT002 PromethION Sequencing Device, PRO-INT002, PromethION Sequencing Device, Sequencing Device, Device
PromethION 2 - Oxford Nanopore Technologies
File Info : application/pdf, 22 Pages, 570.20KB
DocumentDocumentPre-installation
This Quick Start Guide contains everything you need to set up your PromethIONTM 2 Integrated and to check that the device is ready for use.
Before using the device, familiarise yourself with the following:
PromethION 2 Integrated user manual
community.nanoporetech.com/ to/p2i
What's in the box
PromethION 2 Power 2 x Configuration Integrated cables* Test Cells (CTCs)
What you will need
Safety and regulatory information
community.nanoporetech.com/ to/safety
For detailed information and troubleshooting, view the user manual.
DisplayPort/ HDMI-compatible
monitor
Ethernet cable
USB keyboard
USB mouse
* The PromethION 2 Integrated ships with 5 x power cables (1 US, 1 UK, 1 EU, 1 CN, 1 AUS) for international use.
Set up your device
1. Unpack your PromethION 2 Integrated device*
2. Attach the cables and peripherals in the order shown opposite
3. Press the power switch
Rear input/output
Power 3. Ethernet 4. Power
switch port
socket
Touchscreen
Flow cell positions (inside sequencing chamber)
1. HDMI Port or DisplayPort (DP)
2. USB mouse and keyboard
* The device must be placed away from direct sources of heat or sunlight. Place device on a well-supported, strong, clean bench. Allow 30 cm clearance rear and sides, and do not cover ventilation grilles. See user manual for detailed installation advice.
Connect only one monitor.
Log in to your PromethION 2 Integrated
On initial setup, we recommend using an external monitor. For subsequent uses, you can use the touchscreen and/or an external monitor.
Monitor: 1. Log in to your PromethION
Username: prom Password*: prom
2. Open MinKNOWTM Click the wheel icon on the desktop to load MinKNOW.
3. Log into MinKNOW. Use your Oxford Nanopore account details.
* For added security, we recommend changing your password after device setup using the instructions in the user manual.
Log in with your Nanopore account Authorised for offline access?
Note: Follow the tutorials to familiarise yourself with MinKNOW.
Update MinKNOW
For the latest sequencing features, update MinKNOW:
You must now shut down your PromethION 2 Integrated using the instructions in step 4. Once complete, press the power switch and repeat step 2.
Power off
Ensure the device is not performing any tasks and remove any CTCs from the flow cell positions if present.
In MinKNOW, click or press (if using the touchscreen) the top-right power icon, then Shutdown in the pop-up window.
After repeating step 2, you will be able to complete your software update. Then, proceed to step 5.
Oxford Nanopore Technologies phone +44 (0)845 034 7900 email support@nanoporetech.com
@nanopore
www.nanoporetech.com
Oxford Nanopore Technologies, the Wheel icon, MinKNOW and PromethION are registered trademarks of Oxford Nanopore Technologies plc in various countries. All other brands and names contained are the property of their respective owners. © 2024 Oxford Nanopore Technologies plc. All rights reserved. Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition. ONT-08-01121-00-1 | BR_1221(EN)_V1_01Jan2024
Perform hardware check
A hardware check is required before performing your first PromethION 2 Integrated sequencing run. To run a hardware check, follow the on-screen instructions in MinKNOW, then follow the instructions below. You will require your two PromethION Configuration Test Cells (CTCs).
Hardware check overview: 1. Insert the CTCs into the device as shown here and close the lid of the device. 2. In the MinKNOW software, the flow cell status indicators
(the two boxes) will change colour from grey to white. 3. Press Select all available. This will change the colour of the
flow cell status indicators (the two boxes) on the MinKNOW Hardware check panel to dark blue. 4. Press Start in the bottom right. 5. Check the flow cell positions show a to pass the hardware check. 6. Remove the CTCs from the flow cell positions after you complete the hardware check.
Note: If your hardware check fails, see Support in the Additional information section.
Discover the Nanopore Community
community.nanoporetech.com
Ensure the success of your nanopore sequencing project and stay up-to-date with the latest technology and protocols.
Training Protocols Forums Support
FAQs
Tip: Learn how to analyse your nanopore data: nanoporetech.com/analyse
Additional information
Warranty A license and warranty can be purchased for your device here: store.nanoporetech.com/device-warranty.html Flow cell warranty: community.nanoporetech.com/to/warranty
Recycle used flow cells Oxford Nanopore is committed to environmental sustainability. You can help by sending your flow cells for recycling. Find out how: community.nanoporetech.com/support/returns
Place your next order Buy more consumables at the Oxford Nanopore Store: store.nanoporetech.com
Documentation Documentation for your device is available on the Nanopore Community: community.nanoporetech.com/docs
Support For all of your customer and technical support needs, visit: community.nanoporetech.com/support
Technical specification
Model number Supply voltage (V) Maximum rated current (A) Maximum rated power (W) Size (H x W x D) (mm) Weight (kg) Installation ports
Software installed
Compute specification
Environmental conditions
PromethION 2 Integrated
PRO-INT002
AC mains input: 100240 AC ± 10% (50/60Hz)
10 750
180 x 225 x 430 10.6
1 x Ethernet port (2.5 Gbps) 4 x USB 3.0 type-A ports 1 x HDMI port 1 x DisplayPort
Ubuntu MinKNOW, EPI2ME
5.5" touchscreen 15 TB SSD storage 64 GB memory
1 x Intel Core i7 (12-core) 1 x NVIDIA Ampere series GPU
Functional range of electronics is within environmental temperatures of +5°C to +40°C. Users should allow 30 cm clearance to the rear and sides of the device. Designed to sequence in environmental temperatures of +18°C to +25°C. Use within 30%75% relative non-condensing humidity limits. Intended for indoor use. Can be used up to altitudes of 2,000 m. The device has a Pollution Degree 2.
Integrated
Quick start guide
Quick start guide
Multilingual KOREAN | RUSSIAN | ARABIC | |
Contents
Version Korean () 3 Russian () 15 Arabic () 38
ONT-08-01122-00 Rev 1 | BR_1226(ML)_V1_15Mar2024
Quick start guide
KOREAN
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. · PromethION 2 Integrated
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11
(V) (A) (W) (H x W x D)(mm) (kg)
PromethION 2 Integrated
PRO-INT002
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10
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12
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13
Oxford Nanopore Technologies +44 (0)845 034 7900 support@nanoporetech.com
@nanopore
www.nanoporetech.com
Oxford Nanopore Technologies, Wheel , MinKNOW, PromethION Oxford Nanopore Technologies plc . . © 2024 Oxford Nanopore Technologies plc. . Oxford Nanopore Technologies , , , . 14
Quick start guide
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23
PromethION 2 Integrated
PRO-INT002
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Oxford Nanopore Technologies . +44 (0)845 034 7900 support@nanoporetech.com
@nanopore
Oxford Nanopore Technologies
+44 (0)845 034 7900
support@nanoporetechcom
@nanopore
www.nanoporetech.com
Oxford Nanopore Technologies, , GridION, MinKNOW PromethION , Oxford Nanopore Technologies plc . . © 2024 Oxford Nanopore Technologies plc. . Oxford Nanopore Technologies , , , - .
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27
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37
ARABIC
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