Phenolphthalein 1% Solution in Ethanol - Safety Data Sheet

Section 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product Identifiers

Product Name: Phenolphthalein, 1 % solution in ethanol indicator pH 8,2 - 9,8

Catalog Number: 1.07227

Brand: Millipore

UFI: HQ04-Y6GX-X99W-ACHU

REACH Number: This product is a preparation. For REACH registration number, see Chapter 3.

1.2 Relevant Identified Uses of the Substance or Mixture and Uses Advised Against

Identified Uses: Reagent for analysis

Uses Advised Against: This product is not intended for consumer use.

1.3 Supplier Details

Company: Merck Life Science spol.s.r.o

Address: Dvořákovo nábrežie 4, SK-811 08 BRATISLAVA

Phone: +421 2 5557-1562

Fax: +421 2 5557-1564

Email: TechnicalService@merckgroup.com

1.4 Emergency Telephone Number

Emergency Phone: +(421)-233057972 (CHEMTREC)
+421 254774166/911166066 (National Toxicological Information Centre)

Section 2: Hazards Identification

2.1 Classification of the Substance or Mixture

Classification (REGULATION (EC) No 1272/2008):

• Flammable Liquids, Category 3 (H226: Flammable liquid and vapor.)
• Eye Irritation, Category 2 (H319: Causes serious eye irritation.)
• Germ Cell Mutagenicity, Category 2 (H341: Suspected of causing genetic defects.)
• Carcinogenicity, Category 1B (H350: May cause cancer.)

2.2 Label Elements

Hazard Pictograms: ? ⚠️ ☠️

Signal Word: Danger

Hazard Statements:

• H226: Flammable liquid and vapor.
• H319: Causes serious eye irritation.
• H341: Suspected of causing genetic defects.
• H350: May cause cancer.

Precautionary Statements:

• Prevention: P202: Do not handle until all safety precautions have been read and understood. P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P233: Keep container tightly closed. P240: Ground and bond container and receiving equipment.
• Response: P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P308 + P313: If exposed or concerned: Get medical advice/attention.

Hazardous Components for Labeling: Phenolphthalein

Additional Labeling: For professional use only.

Limited Labeling (<= 125 ml):

Section 3: Composition/Information on Ingredients

3.2 Mixtures

Section 4: First-Aid Measures

4.1 Description of First-Aid Measures

General Advice: Show this safety data sheet to a doctor.

If inhaled: Move person to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention.

In case of skin contact: Take off all contaminated clothing immediately. Wash off with plenty of water and soap. Consult a doctor.

In case of eye contact: Rinse thoroughly with plenty of water for at least 15 minutes, lifting lower and upper eyelids. Keep eye wide open while rinsing. If necessary, rinse again. Consult an eye doctor.

If swallowed: Rinse mouth. Do NOT induce vomiting. Never give anything by mouth to an unconscious person. Clean mouth with water and drink afterwards plenty of water. Consult a doctor.

4.2 Most Important Symptoms and Effects, Both Acute and Delayed

Most important symptoms and effects are described on the label (see section 2.2) and/or in section 11.

4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed

No data available.

Section 5: Firefighting Measures

5.1 Extinguishing Media

Suitable Extinguishing Media: Water spray, Alcohol-resistant foam, Dry powder, Carbon dioxide (CO2)

Unsuitable Extinguishing Media: High power water jet

5.2 Special Hazards Arising from the Substance or Mixture

Specific Hazards: Flammable. Vapors are heavier than air and may travel along the ground. Explosive mixtures with air may occur. Combustion products include carbon oxides.

5.3 Advice for Firefighters

Protective Equipment: Wear self-contained breathing apparatus for firefighting.

Further Information: Cool containers with water spray. Prevent fire extinguishing water from entering surface or ground water.

Section 6: Accidental Release Measures

6.1 Personal Precautions, Protective Equipment and Emergency Procedures

For non-emergency personnel: Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Remove all sources of ignition. Evacuate personnel to safe areas.

For emergency responders: Use personal protective equipment as required. See section 8 for information on personal protective equipment.

6.2 Environmental Precautions

Prevent product from entering drains. Danger of explosion.

6.3 Methods and Material for Containment and Cleaning Up

Containment: Cover drains. Soak up with inert absorbent material. Collect contaminated absorbent material.

Cleaning Up: Dispose of contaminated material as waste. Clean contaminated surface thoroughly.

6.4 Reference to Other Sections

For disposal considerations, see section 13.

Section 7: Handling and Storage

7.1 Precautions for Safe Handling

Handling: Work under a fume hood. Avoid inhalation of vapors, aerosols or mist. Keep away from sources of ignition – No smoking. Take precautionary measures against static discharge.

Hygiene Measures: Immediately change contaminated clothing. Use a barrier cream. Wash hands and face after working with the substance.

7.2 Conditions for Safe Storage, Including Any Incompatibilities

Storage Conditions: Keep container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition. Store under lock and key and out of reach of trained or authorized persons.

Storage Class: 3, Flammable liquids

7.3 Specific End Use(s)

Apart from the uses mentioned in section 1.2 no other uses are specified.

Section 8: Exposure Controls/Personal Protection

8.1 Control Parameters

Derived No-Effect Level (DNEL) according to Regulation (EC) No. 1907/2006

Predicted No-Effect Concentration (PNEC) according to Regulation (EC) No. 1907/2006

8.2 Exposure Controls

Personal Protective Equipment:

• Eye/face Protection: Use eye protection tested and approved under appropriate national standards such as NIOSH (US) or EN 166(EU). Safety glasses.
• Skin Protection:
• Hand Protection: Material: Butyl rubber. Breakthrough time: 480 min. Glove thickness: 0.7 mm. For full contact. Manufacturer: Butoject® (KCL 898)
• Hand Protection: Material: Nitrile rubber. Breakthrough time: 120 min. Glove thickness: 0.40 mm. For splash protection. Manufacturer: Camatril® (KCL 730 / Aldrich Z677442, Size M)
• Body Protection: Fire-resistant antistatic clothing.
• Respiratory Protection: Required when vapors or aerosols are generated. Our recommendations for respiratory protective filters are based on the following standards: DIN EN 143, DIN 14387 and other accompanying standards for the use of respiratory protection systems.
• Recommended Filter Type: Filter A (according to DIN 3181) for organic vapors.

Environmental Exposure Controls: Do not allow product to reach sewage system. Danger of explosion.

Section 9: Physical and Chemical Properties

9.1 Information on Basic Physical and Chemical Properties

Form: Liquid

Color: Colorless

Odor: Ethanol

Melting point/freezing point: Data not available

Boiling point or range: Data not available

Flammability: Data not available

Flash point: 23 °C

Auto-ignition temperature: approx. 425 °C

Decomposition temperature: Data not available

pH: 5-7

Viscosity, dynamic: Data not available

Viscosity, kinematic: Data not available

Solubility(ies):

• Water solubility: Soluble (20 °C)

Partition coefficient: n-octanol/water: Data not available

Vapor pressure: Data not available

Density: 0.89 g/cm3 (20 °C)

Relative density: Data not available

Vapor density: Data not available

Particle characteristics: Data not available

9.2 Other Information

Explosives: Not classified as explosive.

Oxidizing properties: None

Section 10: Stability and Reactivity

10.1 Reactivity

Mixtures of vapor with air are explosive on intense heating.

10.2 Chemical Stability

The product is chemically stable under standard ambient conditions (room temperature).

10.3 Possibility of Hazardous Reactions

Hazardous reactions: Risk of explosion/exothermic reaction with:

• Hydrogen peroxide
• Chlorates
• Perchloric acid
• Nitric acid
• Mercury(II) nitrate
• Manganic acid
• Nitriles
• Peroxides
• Strong oxidizing agents
• Nitroso compounds
• Peroxides
• Sodium
• Potassium
• Halogen oxides
• Calcium hypochlorite
• Nitrogen dioxide
• Metal oxides
• Uranium hexafluoride
• Iodides
• Chlorine
• Alkali metals
• Alkaline earth metals
• Alkaline oxides
• Ethylene oxide
• Silver
• Nitric acid
• Silver compounds
• Ammonia
• Potassium permanganate
• Concentrated sulfuric acid

Risk of ignition or formation of flammable gases or vapors with:

• Halogen-halogen compounds
• Chromium(VI) oxide
• Fluorine
• Chromyl chloride
• Hydrides
• Phosphorus oxides
• Platinum
• Nitric acid
• Potassium permanganate

10.4 Conditions to Avoid

Conditions to avoid: Heating.

10.5 Incompatible Materials

Materials to avoid: Rubber, plastics

10.6 Hazardous Decomposition Products

In case of fire: see section 5.

Section 11: Toxicological Information

11.1 Information on Toxicological Effects

Mixture:

• Acute Toxicity: Oral: Data not available. Symptoms: Possible symptoms include irritation of mucous membranes. Dermal: Data not available.
• Skin Corrosion/Irritation: Data not available.
• Serious Eye Damage/Eye Irritation: Causes serious eye irritation.
• Respiratory or Skin Sensitization: Data not available.
• Germ Cell Mutagenicity: Evidence of genetic defects.
• Carcinogenicity: Possible carcinogen.
• Reproductive Toxicity: Data not available.
• Specific Target Organ Toxicity - Single Exposure: Data not available.
• Specific Target Organ Toxicity - Repeated Exposure: Data not available.
• Aspiration Hazard: Data not available.

Substance:

Ethanol:

• Acute Toxicity: Oral LD50 Rat: 10,470 mg/kg. Inhalation LC50 Rat: 124.7 mg/l / 4 h. Dermal: Data not available.
• Skin Corrosion/Irritation: Skin - Rabbit. Result: No skin irritation - 24 h.
• Serious Eye Damage/Eye Irritation: Eyes - Rabbit. Result: Causes serious eye irritation.
• Respiratory or Skin Sensitization: Maximisation test - Guinea pig. Result: negative.
• Germ Cell Mutagenicity: Ames test: negative. In vitro mammalian cell gene mutation test: negative.
• Carcinogenicity: Data not available.
• Reproductive Toxicity: Data not available.
• Specific Target Organ Toxicity - Single Exposure: Data not available.
• Specific Target Organ Toxicity - Repeated Exposure: Data not available.
• Aspiration Hazard: Data not available.

Phenolphthalein:

• Acute Toxicity: Oral: Data not available.
• Skin Corrosion/Irritation: Skin - reconstructed human epidermis (RhE). Result: irritant - 42 min.
• Serious Eye Damage/Eye Irritation: Eyes - In vitro study. Result: non-corrosive - 4 h.
• Respiratory or Skin Sensitization: Skin sensitization - Mouse: Not a skin sensitizer.
• Germ Cell Mutagenicity: Suspected of causing genetic defects. Ames test: negative.
• Carcinogenicity: Substance with suspected carcinogenic effects on humans.
• Reproductive Toxicity: Suspected of damaging fertility.

11.2 Further Information

Endocrine Disrupting Properties (Disruptors): The substance/mixture does not contain components that are classified as endocrine disruptors according to Article 57(f) of REACH Regulation or Commission Delegated Regulation (EU) 2017/2100 or Commission Regulation (EU) 2018/605 at a level of 0.1% or higher.

Other hazardous properties cannot be excluded. Handle with particular caution.

Section 12: Ecological Information

12.1 Toxicity

Substances:

Ethanol:

• Toxicity to fish: LC50 (Pimephales promelas): 15,300 mg/l. Exposure time: 96 h.
• Toxicity to daphnia and other aquatic invertebrates: LC50 (Ceriodaphnia dubia): 5,012 mg/l. Exposure time: 48 h.
• Toxicity to algae: ErC50 (Chlorella vulgaris): 275 mg/l. Exposure time: 72 h.
• Toxicity to microorganisms: IC50 (activated sludge): > 1,000 mg/l. Exposure time: 3 h.
• Chronic toxicity to fish: NOEC: 250 mg/l. Exposure time: 120 h.
• Chronic toxicity to daphnia and other aquatic invertebrates: NOEC: 9.6 mg/l.

Phenolphthalein:

• Toxicity to daphnia and other aquatic invertebrates: EC50 (Daphnia magna): > 100 mg/l. Exposure time: 48 h.
• Toxicity to algae: ErC50 (Desmodesmus subspicatus): > 3.33 mg/l. Exposure time: 72 h. NOEC: 0.57 mg/l.

12.2 Persistence and Degradability

Substances:

Ethanol:

• Biodegradability: Readily biodegradable. Biodegradation: approx. 95%.
• Biochemical oxygen demand (BOD): 930 - 1,670 mg/g.
• Total oxygen demand (TOD): 2,100 mg/g.

Phenolphthalein:

• Biodegradability: Readily biodegradable. Biodegradation: 76%.

12.3 Bioaccumulative Potential

Substances:

Ethanol: No bioaccumulation is to be expected due to the low n-octanol/water partition coefficient.

Phenolphthalein: No bioaccumulation is to be expected.

12.4 Mobility in Soil

Data not available.

12.5 Results of PBT and vPvB Assessment

Product: The substance/mixture does not contain components that are considered to be persistent, bioaccumulative and toxic (PBT) or very persistent and very bioaccumulative (vPvB) in quantities of 0.1% or more.

Substances:

Ethanol: The substance does not meet the criteria for PBT or vPvB according to Regulation (EC) No. 1907/2006, Annex XIII.

12.6 Endocrine Disrupting Properties (Disruptors)

Product: The substance/mixture does not contain components that are suspected of having endocrine disrupting properties.

12.7 Other Adverse Effects

Substances:

Phenolphthalein:

• Additional ecological information: Data not available.

Section 13: Disposal Considerations

13.1 Waste Treatment Methods

Product: Waste must be disposed of in accordance with federal, state and local environmental control regulations. Keep chemical in original containers. Do not mix with other waste. Treat empty containers as the product itself.

Section 14: Transport Information

14.1 UN Number or Identification Number

• ADR: UN 1170
• IMDG: UN 1170
• IATA: UN 1170

14.2 UN Proper Shipping Name

• ADR: ETHANOL SOLUTION
• IMDG: ETHANOL SOLUTION
• IATA: Ethanol solution

14.3 Transport Hazard Class(es)

• ADR: 3
• IMDG: 3
• IATA: 3

14.4 Packing Group

• ADR: III
• IMDG: III
• IATA: III

14.5 Environmental Hazards

• ADR: No
• IMDG: No

14.6 Special Precautions for User

The transport classification(s) provided herein are for informational purposes only and are based on the properties of the unpacked material as submitted. It is the responsibility of the transporting organization to determine the appropriate transport classification, packaging, and labeling based on the mode of transport, the quantity, and the specific requirements of the applicable regulations.

14.7 Maritime transport in bulk according to Annex II of MARPOL and the IBC Code

Not applicable for the product supplied.

Section 15: Regulatory Information

15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance or Mixture

• REACH - Restrictions on the manufacture, placing on the market and use of certain dangerous substances, mixtures and articles (Annex XVII): Conditions of restriction apply to the following entries: Entry 3, Entry 28: Phenolphthalein, Entry 75: If you intend to use this product as a tattoo ink, please contact your supplier.
• REACH - Candidate List of Substances of Very High Concern (SVHC) which are on the Authorisation List (Article 59): Phenolphthalein
• Regulation (EC) No 2037/2000 on substances that deplete the ozone layer: Not applicable
• Regulation (EU) 2019/1021 on persistent organic pollutants (recast): Not applicable
• REACH - List of Substances subject to Authorisation (Annex XIV): Not applicable
• Seveso III Directive: Not applicable

15.2 Chemical Safety Assessment

A chemical safety assessment has not been carried out for this product.

Section 16: Other Information

16.1 Full Text of H-Statements referred to under Sections 2 and 3

• H225: Highly flammable liquid and vapor.
• H315: Causes skin irritation.
• H319: Causes serious eye irritation.
• H341: Suspected of causing genetic defects.
• H350: May cause cancer.
• H361f: Suspected of damaging fertility.

16.2 Full Text of Abbreviations and Acronyms

• Carc.: Carcinogenicity
• Eye Irrit.: Eye irritation
• Flam. Liq.: Flammable liquids
• Muta.: Germ cell mutagenicity
• Repr.: Reproductive toxicity
• Skin Irrit.: Skin irritation
• SK OEL: Slovak Occupational Exposure Limit
• SK OEL / NPEL average: SK OEL / NPEL average
• SK OEL / NPEL short-term: SK OEL / NPEL short-term
• ADN: European Agreement concerning the International Carriage of Dangerous Goods by Inland Waterways
• ADR: European Agreement concerning the International Carriage of Dangerous Goods by Road
• AIIC: Australian Inventory of Industrial Chemicals
• ASTM: American Society for Testing and Materials
• bw: body weight
• CLP: Regulation (EC) No 1272/2008 on classification, labelling and packaging of substances and mixtures
• CMR: Carcinogenic, mutagenic or toxic for reproduction
• DIN: German Institute for Standardization
• DSL: Canada's Domestic Substances List
• ECHA: European Chemicals Agency
• EC-Number: European Community number
• ECx: Concentration of substance causing x% effect
• ELx: Rate of loading of substance causing x% effect
• ENCS: Existing and New Chemical Substances (Japan)
• ErCx: Concentration of growth rate change x%
• GHS: Globally Harmonized System of Classification and Labelling of Chemicals
• GLP: Good Laboratory Practice
• IARC: International Agency for Research on Cancer
• IATA: International Air Transport Association
• IBC: International Code for the Construction and Equipment of Ships carrying Dangerous Chemicals in Bulk
• IC50: Half maximal inhibitory concentration
• ICAO: International Civil Aviation Organization
• IECSC: Inventory of Existing Chemical Substances in China
• IMDG: International Maritime Dangerous Goods Code
• IMO: International Maritime Organization
• ISHL: Industrial Safety and Health Law (Japan)
• ISO: International Organization for Standardization
• KECI: Korean Existing Chemicals Inventory
• LC50: Lethal Concentration to 50% of test population
• LD50: Lethal Dose to 50% of test population (median lethal dose)
• MARPOL: International Convention for the Prevention of Pollution from Ships
• n.o.s.: not otherwise specified
• NO(A)EC: Not observed (adverse) effect concentration
• NO(A)EL: Not observed (adverse) effect level
• NOELR: No observed effect rate of loading
• NZIoC: New Zealand Inventory of Chemicals
• OECD: Organisation for Economic Co-operation and Development
• OPPTS: Office of Chemical Safety and Pollution Prevention
• PBT: Persistent, bioaccumulative and toxic
• PICCS: Philippine Inventory of Chemicals and Chemical Substances
• (Q)SAR: (Quantitative) Structure-Activity Relationship
• REACH: Regulation (EC) No 1907/2006 of the European Parliament and of the Council concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals
• RID: Regulations concerning the International Carriage of Dangerous Goods by Rail
• SADT: Self-Accelerating Decomposition Temperature
• SDS: Safety Data Sheet
• SVHC: Substance of Very High Concern
• TCSI: Taiwan Chemical Substance Inventory
• TECI: Inventory of Existing Chemical Substances in Thailand
• TRGS: Technical Rules for Dangerous Substances
• TSCA: Toxic Substances Control Act (United States)
• UN: United Nations
• vPvB: Very Persistent and Very Bioaccumulative

16.3 Disclaimer

The information provided in this safety data sheet is compiled to the best of the manufacturer's knowledge and is intended to be used as a guide. Sigma-Aldrich Co. and its affiliates do not accept liability for any damages resulting from the handling or use of the chemicals mentioned. Therefore, please adhere to the terms and conditions of sale available on www.sigma-aldrich.com and/or on the back of invoices and leaflets.

Copyright 2025 Sigma-Aldrich Co. LLC. License granted to produce any quantity of paper copies for internal use.

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