iScope Polarisation

Stage Rotation

When the stage rotation clamping knob (1) is loosened, the stage can be rotated 360 degrees horizontally.

Diagram: Microscope stage with a labeled clamping knob (1) for 360-degree rotation.

2.1 Using the Bertrand Lens

By revolving the Bertrand lens dial (3), the Bertrand lens can be selected. At the "O" position, the lens is removed from the light path. At the "B" position, the lens is engaged.

Diagram: Microscope head showing the Bertrand lens dial (3).

2.2 Focusing the Bertrand Lens

During conoscopic observation, to focus the conoscopic image, turn the Bertrand lens focusing ring (4) slightly until a clear interference image is obtained in the eyepiece.

Diagram: Microscope head showing the Bertrand lens focusing ring (4).

3.1 Centering the Objectives

All 360° centering objectives of the Euromex IS-series material microscopes are pre-centered in our factory. However, during transportation or after a long period of inactivity, the centering of these objectives may have been shifted.

Please follow the steps below in order to re-center the objectives of a polarization microscope:

1. Remove one eyepiece from the microscope head.
2. Insert the widefield eyepiece with a crosshairs into the tube of the eyepiece you just removed.
3. Position a microscope slide with a crosshairs reticule under the stage clamps.
4. Check if the 4x objective is positioned in the optical path.
5. Position the round stage with the vernier on its "0" position.
6. Position the middle of the crosshairs of the microscope slide on top of the crosshairs of the eyepiece (a).
7. Rotate the stage by 180°. A displacement might be observed (b).
8. Move by hand, the middle of the crosshairs of the microscope slide, approximately half way to the eyepiece crosshairs (c).
9. Rotate the stage back to its "0" position.
10. The 4x objective is equipped with two adjustable screws inside the revolving nosepiece for centering the objective. Use the centering screws to move the center of the eyepiece crosshairs towards the center of the crosshairs of the microscope slide.
11. Repeat steps 7 to 10 until the objective is centered.
12. Repeat for the other objectives using the same procedure.

Diagrams illustrating objective centering: (a) Initial alignment of slide crosshairs with eyepiece crosshairs. (b) Observed displacement after rotating the stage 180°. (c) Manual adjustment of the slide crosshairs towards the eyepiece crosshairs.

If the centering cannot be done correctly:

1. Check if the 4x objective is set in the central position of the centering screw's correction range. This means that when the centration is changed by using the screws in the revolver, the cross of the slide should be able to move to all directions in an equal amount.
2. Repeat this for the other objectives.
3. If the centering is still unsuccessful, please check if the mechanical stage is correctly centered. The stage is fixed by four screws at the bottom of the stage. Untighten the screws so the stage can be moved and align the stage around the lens of the condenser visually. Note that the condenser should first be aligned in the correct manner, the procedure is described in the iScope user manual.