Euromex iScope Fluorescence Microscope

HIGHLIGHTS

• HWF 10x/22 mm eyepieces
• Binocular and trinocular models
• Optional ergonomic tilting head
• Quintuple revolving nosepiece
• E-Plan, plan and plan fluarex corrected IOS objectives
• 216 mm x 150 mm rackless stage with integrated X-Y stage
• Optional ceramic stage
• Height adjustable Abbe condenser N.A. 1.25
• Coarse and fine adjustment
• Diascopic intensity adjustable 3 W NeoLED™ Köhler illumination
• Epi-fluorescence 100 W HBO mercury vapor
• Epi-fluorescence 460-495 nm or 510-550 nm 3 W LED
• iCare sensor for energy saving
• CSS - Cable Storage System
• 10 years warranty

TECHNICAL SPECIFICATIONS

EYEPIECE(S)

• Extended EWF 10x/22 mm eyepieces, Ø 30 mm tubes.
• Eyepieces can be secured to the tubes.

HEADS

• Binocular and trinocular Siedentopf type heads with 30° inclined tubes, interpupillary distance from 48 to 76 mm and equipped with diopter adjustment(s).
• A rotating system allows ergonomic positioning of both tubes in a high (431 mm) and low (397 mm) position.
• The trinocular head comes with a Ø 23.2 mm photo port.
• An optional ergonomic tilting head and corresponding Ø 23.2 mm photo tube attachment are also available (only infinity corrected models). To order an iScope with tilting head use reference IS.3158-xxx.

NOSEPIECE

• Revolving and reversed nosepiece for maximum 5 objectives.

OBJECTIVES

The iScope® offers a range of objectives including E-Plan EPLI IOS, Plan PLI IOS, and Plan PLFI IOS. The Plan PLFI™™™ objectives are suitable for Violet and Ultra Violet excitation. All optics are anti-fungus treated and anti-reflection coated for maximum light throughput.

STAGES

• 216 x 150 mm rackless stage equipped with double slide holder and integrated 79 x 52 mm mechanical stage. This stage has no protruding parts for safer movements.
• 220 x 157 mm ceramic stage with integrated 75 x 50 mm mechanical stage, featuring a tough ceramic surface that minimizes stray light reflection.

CONDENSER FOR BRIGHTFIELD

• Standard height adjustable Abbe N.A. 1.25 condenser for brightfield, suitable for plan phase 10x/20x or S40x/S100x oil immersion IOS objectives for economical phase contrast.
• Magnification indication.
• A slider with a simple dark stop for dark field up to 400x magnification is available.

CONDENSER FOR ZERNIKE PHASE CONTRAST

• Optional height adjustable Zernike NA. 1.25 phase contrast disc condenser with plan phase 10/20/S40x and S100x oil immersion objectives, and dark field stop position for dark field contrast up to 400x magnification.

FOCUSING

• Coaxial coarse and fine adjustments with 200 graduations, 1 µm per graduation, 200 μm per rotation, and approximately 24 mm total travel.
• Supplied with an adjustable rack stop to prevent damage to sample and objectives.
• Coarse adjustments are equipped with friction control.

ILLUMINATION

• 3 W adjustable Köhler NeoLED™ diascopic illumination with internal 100-240 V power supply. The NeoLED™ design offers larger apertures for higher resolutions, approaching the theoretical diffraction limit. Benefits include low energy consumption, no heating, and a long operating lifetime. Köhler illumination on models with IOS plan and plan phase infinity corrected system provides high contrast and maximum achievable resolving power.

ILLUMINATION FOR FLUORESCENCE

• 6-positions rotating or 3-positions slider fluorescence attachments are supplied with a standard 100 W HBO mercury vapor light source for broad, powerful light spectrum and 100-240 V external power supply with lamp lifetime indicator. Supplied with standard fluorescence filters sets for blue and green excitation and a UV protection shield.
• The iScope® fluorescence microscopes can be supplied with more specific, optimized, and application-related fluorescence filter sets.
• Two single-position fluorescence attachments are supplied with 460-495 nm 3 W LED for blue excitation or 510-550 nm 3W LED for green excitation, with 100-240 Vac/7.5 Vdc external power supply. Supplied with standard fluorescence filters sets for blue and green excitation.

STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS

The following filter sets are available:

• IS.9745: Blue excitation filter set (EX: 460-490 nm bandpass, DM: 520 nm, EM: 500 nm longpass)
• IS.9746: Green excitation filter set (EX: 510-550 nm bandpass, DM: 580 nm, EM: 570 nm longpass)
• IS.9747: UV excitation filter set (EX: 330-385 nm bandpass, DM: 420 nm, EM: 400 nm longpass)
• IS.9748: Violet excitation filter set (EX 400-410 nm bandpass, DM 455 nm, EM 455 nm longpass)

ICARE SENSOR

The iCare Sensor is designed to prevent unnecessary energy loss by automatically switching off the illumination shortly after the microscopist steps away.

CSS - CABLE STORAGE SYSTEM

• The iScope® allows users to easily insert the power cable into the backside of the instrument for convenient storage.
• An integrated carrying grip at the back ensures safe transportation.

PACKAGING

• Supplied with power cord, dust cover, a spare fuse, user manual, and 5 ml immersion oil for models with S100x objective.
• Models with 100 W mercury vapor light source include a UV protection shield.
• All microscopes are packed in polystyrene boxes.

MODELS

Euromex offers various iScope fluorescence microscope models, including:

• IS.3153 PLi/LB
• IS.3152 PLi-3
• IS.315 PLFi-6

SPECIFICATIONS (INFINITY)

ACCESSORIES AND SPARE PARTS

OPTIMIZED FLUORESCENCE FILTERS SETS (Only for models with 100 W mercury vapor illuminations)

For fluorescence filter sets for the 6-positions attachment, add /6 to the reference. For fluorescence filter sets for the 3-positions attachment, add /3 to the reference.

• IS.9750.0401: Premium filter set EX440 (BP21), DM455 (LP), EM480 (BP30) CFP, Cerulean, CyPet, SpectrumAqua. Needs empty filter block.
• IS.9750.39001: Filter set EX435 (BP20) DM455 (LP) EM480 (BP30) AmCyan, Atto 425, Cerulean, CFP, ECFP fluorophore. Needs empty filter block.
• IS.9750.39002: Filter set EX480 (BP30) DM505(LP) EM535 (BP40) Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™™™ 488, SYBR Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block.
• IS.9750.0403: Premium filter set EX380 (BP50), DM410 (LP), EM450 (BP60) BFP, BFP2, DAPI, Alexa Fluor 350, Hoechst 33342 & 33258, BFP. Needs empty filter block.
• IS.9750.0404: Premium filter set EX470 (BP40), DM500 (LP), EM535 (BP50) Alexa Fluor 488, CoralHue Azami Green, Cy2, eGFP, FITC, GFP (sapphire), Emerald. Needs empty filter block.
• IS.9750.39003: Filter set EX495 (BP20) DM515 (LP) EM540 (BP30) Alexa Fluor 514™™, Citrine, EYFP/pH 7, F/IAsH-CCPFCC, mCitrine, Oregon Green™ 514, Rhodamine 123, Topaz. Needs empty filter block.
• IS.9750.0407: Premium filter set EX635 (BP30), DM660 (LP), EM710 (BP80) Cy5, Alexa Fluor 647. Also compatible with TO-PRO-3, TOTO-3, Kodak, X-Sight 650. Needs empty filter block.
• IS.9750.39004: Filter set EX540 (BP25) DM565 (LP) EM605 (BP55) Alexa Fluor 546™, Alexa Fluor 555™, Atto 550, CAL Fluor® Red 590, Cy3™, Dil, DsRed, DyLight 549, MitoTracker Orange/MeOH, Resorufin, Rhod-2, TagRFP, TAMRA, tdTomato, Tetramethylrhodamine isothiocyanate, TRITC. Needs empty filter block.
• IS.9750.39005: Filter set EX540 (BP25) DM565 (LP) EM 620 (BP60) TRITC-red-shifted, Ethidium homidimer-1/DNA, Propidium Iodide. Needs empty filter block.
• IS.9750.39007: Filter set EX620 (BP50) DM655 (LP) EM 690 (BP50) Alexa Fluor 647™™, Allophycocyanin (APC), Atto 647N, Cy5™, DiD, Draq5, DayLight 649, MitoTracker Deep Red 633/MeOH, Nile Blue, Quasar® 670, SYTO® 60, TO-PROM-3. Needs empty filter block.
• IS.9750.0412: Premium filter set EX500 (BP25), DM252 (LP), EM545 (BP35) Acridine Orange (+DNA), Rhodamine Green, CL NERF, (hi pH), YFP. Also compatible with Alexa Fluor 532. Needs empty filter block.
• IS.9750.0414: Premium filter set EX560 (BP55), DM505 (LP), EM645 (BP75) Texas Red, Texas Red-X, CryptoLight CF-6. Also compatible with Cy3.5, HcRed, mRaspberry, MitoTracker Red. Needs empty filter block.

OPTIMIZED PREMIUM FLUORESCENCE FILTERS SETS (Only for models with 100 W mercury vapor illuminations)

• IS.9749-6: Empty filter cube for 6-positions fluorescence attachment.
• IS.9749-3: Empty filter cube for 3-positions fluorescence attachment.
• IS.9749-1: Empty filter cube for 1-position fluorescence attachment.
• IS.9753: Filter rail, 3-positions.

ALL MODELS

• IS.9102: Standard Abbe condenser 1.25 NA condenser with slot for dark field and phase contrast sliders.
• IS.9105: Swing-out 0.9/1.25 NA condenser.
• SL.5500: LED replacement unit for iScope.
• AE.5130: Universal SLR camera adapter with 2x projection lens for 23.2 mm tubes. Needs T2 adapter.
• AE.5025: T2 adapter for Nikon D digital SLR cameras.
• AE.5040: T2 adapter for Canon EOS digital SLR cameras.
• PB.5155: Microscope slides 76 x 26 mm, ground edges, 50 pieces.
• PB.5165: Cover glasses 18 x 18 mm, thickness 0.13-0.17 mm, 100 pieces.
• PB.5168: Cover glasses 22 x 22 mm, thickness 0.13-0.17 mm, 100 pieces.
• PB.5245: Lens cleaning paper, 100 sheets per pack.
• PB.5255: Immersion oil, n = 1.482 (25 ml).
• PB.5274: Isopropyl alcohol 99% (200 ml).
• PB.5275: Cleaning kit: lens cleaning fluid, lint free lens tissue, brush, air blower, cotton swabs.

Certifications

Euromex Microscopen bv is certified under ISO 9001:2008 by TÜV Rheinland.