SureTrend® Quantification Calculator for the BAX® System

SureTrend® Quant

Getting Started

1. Following the completion of the BAX® System Real-Time PCR result analysis, ensure the file is saved in a known location as a .bax file. Log into SureTrend.

A screenshot of the SureTrend login page is displayed, featuring input fields for 'Username' and 'Password', a 'reCAPTCHA' verification, and buttons for 'Log in', 'Create new account', 'Sign in with SSO', and 'Sign in with Google'.

Quant Setup (Only at First Use of SureTrend Quant)

2. To log into a SureTrend account with the Premium package, navigate to 'Settings' (indicated by a gear icon ⚙️). Under 'Account Management', ensure that the Package type is set to 'Premium'. If you have a starter package, click on the 'Upgrade' button and complete a form to access the Quant Online software.

A screenshot of the SureTrend interface is shown. It highlights the 'Settings' section, the 'Account Management' area within settings, the 'Package' type displayed as 'Premium', and an 'Upgrade' button.

Enabling Quant and File Upload

3. To enable Quant, navigate to the 'Sites' tab. Select a site. Click on the 'Site Settings' tab. Under 'Product Selections', click on 'PCR'. Check the 'Quant' checkbox. Your account is now set up to use Quant in future data analyses.

A screenshot of the SureTrend 'Site Settings' page is shown. It displays navigation tabs, a 'Default Site' dropdown, and a 'Product Selections' section. Within 'Product Selections', 'ATP', 'Microorganisms by Sample', 'Enzymes', 'Allergens', and 'PCR' are listed. The 'PCR' option is selected, revealing a checkbox labeled 'Quant' which is checked (indicated by a checkmark ✔️).

After registering with SureTrend, BAX Q7 desktop computers with software 5.0 or later can be enabled to automatically upload files to SureTrend. Files may also be uploaded manually by following the steps below.

4. To upload files manually, navigate to the 'Quant' tab. Click on the 'Upload Files' button.

A screenshot of the SureTrend 'Quant' tab is shown, with an 'Upload Files' button highlighted.

Manual File Upload Process

5. Select the site from the dropdown list. Select 'Bax (.bax)' for the File Type. Ensure the box is checked for the question, 'Is this Quant upload?'. Press the 'Select file(s) to upload' button. Select .bax file(s) from your saved location. Note: Multiple files can be uploaded at once.

A screenshot of the SureTrend 'PCR Data Upload' interface is displayed. It includes a 'Select Site' dropdown, a 'File Type' dropdown, a checkbox labeled 'Is this Quant upload?' which is checked, and a button labeled 'Select file(s) to upload'. Below this, a table lists previously uploaded files.

6. Press the 'Upload' button to upload all the files that appear under the 'Upload' button. Files can be uploaded individually by clicking on the upload icon next to the file name. Files can be removed from the list by clicking on the delete icon next to the file name.

A screenshot of the SureTrend 'PCR Data Upload' page is shown, with a list of files. Arrows indicate the main 'Upload' button, an individual file upload icon (a circular arrow symbol ?), and a delete icon (a trash can symbol ?️) next to each file.

View and Export BAX Quant Results

7. When the Status changes from 'Uploading...' to 'View rack', the file has been uploaded successfully. Click on 'View rack' to open the PCR results table.

A screenshot of the SureTrend 'PCR Data Upload' page shows a file with its status updated to 'View rack'. An arrow points to the 'View rack' link.

8. Select the rows you want to view in more detail by checking the boxes in the first column. The rows will become highlighted in gray. Click on the 'Quant results' button.

A screenshot of the SureTrend 'Rack View' is displayed. It shows a table of results with checkboxes in the first column, allowing users to select specific rows for detailed viewing. An arrow points to the 'Quant results' button.

9. In the rack view, select individual wells for analysis by clicking on the name or use the 'Select/Unselect All' blue icon. From the Quantification Matrix Selection under the rack view, select the industry name to open the list of matrices and select the matrix. Click on the blue icon in the top right corner of the screen and select 'Analyze' from the dropdown menu.

A screenshot illustrates the 'Rack View' with selectable wells, indicated by circular icons. It also shows the 'Quantification Matrix Selection' panel, where users can select an 'Industry Name' (e.g., Poultry, Environmental) and then a specific 'Matrix' (e.g., Ground Chicken, Ground Turkey). An arrow points to a dropdown menu in the top right corner, showing 'Analyze' as a selectable option.

10. The graphical results of the selected wells will be displayed.

A graph displays the graphical results for selected wells, showing multiple curves representing quantification data over time.

11. View the table under the graphs for additional details and to export Quant results. Estimations (Estimated Log CFU per Sample) will be displayed in multiple unit formats to be used at the user's discretion. Click on the Column Chooser icon to open the selection box and choose the data that you want to export. Click on the export button to download a .csv file.

A screenshot shows a detailed results table below a graph. The 'Estimated Log CFU per Sample' column is highlighted. A 'Column Chooser' dialog box is shown, allowing users to select which data columns to display. An arrow points to an 'export' button (download icon ⬇️) for downloading data as a .csv file.

Technical Assistance

For detailed troubleshooting on the BAX System instrument, see the BAX System Q7 User's Guide. For instructions for specific matrices and pathogen targets when using the BAX System, see the Quantification and Limits Manual. Guides and manuals are available at www.hygiena.com/documents.

If you have any additional questions or comments on the BAX System or quantification procedures, contact Hygiena Diagnostics Support by email at diagnostics.support@hygiena.com.