MinION Mk1D Portable Nanopore Sequencing Device User Guide

Install the latest MinKNOW software for MinION Mk1D and P2 Solo from the official website.
Log in to MinKNOW using your Oxford Nanopore account details.v

Setup Your MinION Mk1D

Connect the MinION to your computer using the provided USB-C cable.

Insert the Configuration Test Cell (CTC) by following the provided instructions.

Performing a Hardware Check

Initiate a hardware check within the MinKNOW software.
Follow the on-screen instructions to complete the hardware check.

Ensure the flow cell position in MinKNOW shows “Hardware check passed” after completion.

Additional Information
Explore the Nanopore Community for further support, protocols, forums, and downloads. Utilize available resources for warranty, recycling, ordering consumables, documentation, and support.

FAQ

Q: What should I do if the hardware check fails?
A: If the hardware check fails, ensure the CTC is fully inserted and repeat the hardware check. If issues persist, contact technical support using the details provided in the guide.

Q: How can I recycle used flow cells?
A: Oxford Nanopore promotes environmental sustainability by accepting used flow cells for recycling. Visit the Nanopore Community to learn how to send your flow cells for recycling.

Pre-installation

This Quick start guide contains everything you need to set up your MinION™ Mk1D and to check that the device is ready for use.

Before using the device, familiarise yourself with the following:

IT requirements community.nanoporetech.com/to/minion-it

MinION Mk1D user manual community.nanoporetech.com/to/minmk1d
Safety and regulatory information community.nanoporetech.com/to/safety

For detailed information and troubleshooting, view the user manual.

MinION Mk1D

Install and log in to MinKNOW™

Check your computer meets the IT requirements.

Install the latest MinKNOW for the MinION Mk1D and P2 Solo from community.nanoporetech.com/downloads. Once installed, open MinKNOW.
Log in to MinKNOW using your Oxford Nanopore account details.

Set up your MinION Mk1D

Connect the MinION to your computer using the USB-C cable*.

Insert your CTC

Lift the lid of the MinION.
Insert the Configuration Test Cell (CTC) a .
Ensure you press down firmly on the CTC b . Then close the MinION lid.

Perform a hardware check

A hardware check is needed before your first sequencing run. To run a hardware check, follow the instructions below:

Click Start in the left side bar.
Click hardware check.
Click Start in the bottom right corner of MinKNOW. Wait for the hardware check to complete.
Check the flow cell position in MinKNOW shows “Hardware check passed” √.
Remove the CTC after you’re finished.

*If the hardware check fails, ensure your CTC is fully inserted, then repeat the hardware check. Use the details in Additional information in this guide to contact technical support if your hardware check fails again.

Discover the Nanopore Community

community.nanoporetech.com
Discover the Nanopore Community to find protocols, forums, support and downloads for your experiments.

Tip: Learn how to analyse your nanopore data at: nanoporetech.com/analyse

Additional information

Warranty
A license and warranty can be purchased for your device here: store.nanoporetech.com/device-warranty.html Flow cell warranty: community.nanoporetech.com/to/warranty

Recycle used flow cells
Oxford Nanopore is committed to environmental sustainability. You can help by sending your flow cells for recycling. Find out how: community.nanoporetech.com/support/returns

Place your next order
Buy more consumables at the Oxford Nanopore Store: store.nanoporetech.com

Documentation
Documentation for your device is available on the Nanopore Community: community.nanoporetech.com/docs

Support
For all of your customer and technical support needs, visit: community.nanoporetech.com/support

Technical specification

Model number	MIN-101D	Weight (g)	130
Supply voltage (V)	5 DC	Installation ports	1 x USB-C
Maximum rated current (A)	1.5	Software installed	MinION driver
Maximum rated power (W)	7.5	Compute specification	N/A
Size (H x W x D) (mm)	13 x 55 x 125
Environmental conditions	Designed to sequence in environmental temperatures of +10°C to +35°C. Functional range of electronics is within environmental temperatures of +5°C to +40°C. 5 cm clearance around the device is needed (for ventilation and temperature control) when in use. Use within 30%–75% relative non-condensing humidity limits. Intended for indoor use. Can be used up to altitudes of 2,000 m. The device has a Pollution Degree 2.

Oxford Nanopore Technologies

phone +44 (0)845 034 7900

email support@nanoporetech.com
X @nanopore

Gosling Building Edmund Halley Road Oxford Science Park OX4 4DQ, UK

www.nanoporetech.com
Oxford Nanopore Technologies, the Wheel icon, MinION, and MinKNOW are registered trademarks of Oxford Nanopore Technologies in various countries. © 2024 Oxford Nanopore Technologies. All rights reserved. Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.
ONT-08-01144-00 Rev 2 | BR_1229(EN)_V2_01Oct2024

Documents / Resources

	MinION Mk1D Portable Nanopore Sequencing Device [pdf] User Guide Mk1D Portable Nanopore Sequencing Device, Mk1D, Portable Nanopore Sequencing Device, Nanopore Sequencing Device, Sequencing Device, Device
	MinION Mk1D Portable Nanopore Sequencing Device [pdf] User Guide Mk1D, Mk1D Portable Nanopore Sequencing Device, Mk1D, Portable Nanopore Sequencing Device, Nanopore Sequencing Device, Sequencing Device, Device