Implement NPOS Software NanoPhotometer

Version History NPOS Software

• Software Version 4.6n build 16350
• Valid from 04.10.2019 last change 27.05.2025
• NPOS Version 4.6n 16350 (28.05.2025)

Improvements

• Automatic System Check on Startup: If the device detects a read-only state on startup, the system now automatically runs a repair check to maintain stability and prevent file system issues.
• Support for WORM Network Server: Our software now supports WORM (Write Once, Read Many) network drives.
• Optimized background checks prevent write operations that could cause file system errors, improving compatibility and ensuring data integrity compliance.

NPOS Version 4.6m 16350 (28.01.2025)

Implemented Functions
N120 single channel: shows always lid 80 in case of ABR

 Improvements

• Check all network folders every 5minutesn to keep them alive
• N120: Do not move the horizontal motor with the maintenance stick
• During Linux setup, set eMMC to pSLC mode and enable automatic eMMC BKOPS

Resolved Issues
In the case of Air Bubble Recognition, the corrected wrongly shown lid is 80 / 140.

NPOS Version 4.6L 16350 (19.11.2024)

CFR21 Software

• CFR21: audit trail for backup missing

Improvements

• Add a new screensaver video
• Implement eMMC maintenance
• Fix Japanese translations
• Check all network folders every 5minutesn to keep them alive

NPOS Version 4.6k 16350 (13.06.2024)

Implemented Functions
General (affects all NanoPhotometer® models):

• N/A

NPOS Version 4.6j 16350 (29.04.2024)

Implemented Functions
General (affects all NanoPhotometer® models):

• Nucleic Acids and Concentration Methods: New measurement unit added (nmol/l)
• Automatic Date and Time synchronization via NTP server
• Stored Results: New back button added when IDS/JSON files are opened

CFR21 Software

• Additional CFR21 role added: Viewer – for auditing purposes
• New preference added: Administrators can enable/disable permission for Power Users to add new Users.

Improvements
WiFi functionality improved: Expanded list of SSIDs displayed

Resolved Issues

• Air Bubble Recognition function improved
• HP printer support has been upgraded

NPOS Version 4.6f 16350 (26.04.2023)

Implemented Functions

• N/A

Improvements

• N/A

Resolved Issues
General (affects all NanoPhotometer models)

• The kinetics application improved the duration of the kinetics measurements
• Intermittent disruptions in generating PDF reports have been resolved

NPOS Version 4.6e 16350 (30.01.2023)

Implemented Functions

• N/A

improvements

• N/A

Resolved Issues
Kinetics: Added missing special characters as sample names

NPOS Version 4.6d 16350 (20.01.2023)

Implemented Functions
General (affects all NanoPhotometer models):

• Extend REST API Push Service for general LIMS integration
• NanoPhotometer® N120:
• N120: New multi-channel method: Wavelength

Improvements
General (affects all NanoPhotometer models):

• Make remote access via PageKite configurable via a magic file
• Additional special characters available as sample names
• Kinetics: rounding of Slope value and duration

Updated HP Printer Driver to HPLIP 3.22.10

Resolved Issues
General (affects all NanoPhotometer models):

• Stored Results: handling of dot during file rename

NanoPhotometer® N120
Wavescan: Opening the lid in an 80 measurement

NPOS Version 4.6c 16350 (25.08.2022)

Implemented Functions
NanoPhotometer® N120:
Protein Assay multichannel mode: New assay type Custom

Improvements
General (affects all NanoPhotometer models):
Compatibility for Dymo 550 and 5XL printer

Resolved Issues
General (affects all NanoPhotometer models):
Test dyes removed from preprogrammed dye list

NanoPhotometer® N120

• Windows App: Fix in Protein Assay tab bar
• Warning message: Fix for editing more than 12 sample names

NPOS Version 4.6b 16350 (21.07.2022)

Implemented Functions
N/A

Improvements
N/A

Resolved Issues
NanoPhotometer® N120:
Wrong dilution factors are shown in the graphical user interface after reset.

NPOS Version 4.6a 16350 (20.04.2022)

Implemented Functions
N/A

Improvements
Updated HP printer driver

Resolved Issues
N/A

NPOS Version 4.6 16350 (07.03.2022)

Implemented Functions
N/A

Improvements
N/A

Resolved Issues

• Copy/move data to a USB flash drive with too little storage
• Closing/opening cuvette slider (NP80)

NPOS Version 4.5b 16350 (16.02.2022)

Implemented Functions

• N/A

Improvements
N/A

Resolved Issues
NanoPhotometer® N120:
N120: Configuration Change

NPOS Version 4.5 16350 (27.01.2022)

Implemented Functions
N/A

Improvements
General (affects all NanoPhotometer models):
Available SMB protocols: SMB 3.1, SMB 3.0, SMB 2.1, SMB 2.0, and SMB 1.0 (hardware version C.2)

Resolved Issues

General (affects all NanoPhotometer models):

• Assistance/Selftest: Folder list not shown in the save dialog
• Handling of initialization / selftest with lid open

Protein Assay / Standard Curve method: scaling of the axis
Apps for control devices:

• Mac: OD600 method: selection options for multiplier parameter not shown
• Android Tablet: Toggle switches in Protein Assay and Standard Curve sometimes do not work
• Android Tablet: Stored Results/Methods: Files sometimes not shown

NPOS Version 4.4 16326 (22.12.2021)

Implemented Functions
CFR21 Software:

• Search Option in Audit Trail
• Audit trail available on touchscreen, sorted by year. Saving possible up to 50.000 entries. No direct printing of audit trail in NPOS software.

Improvements
General (affects all NanoPhotometer® models):

• Change of the default dye parameter according to the dye manufacturer
• Improved screensaver with a new movie
• Improved translations
• Improvement of rounding numbers
• New and improved warning messages for easier handling

CFR21 Software

• Improvement of Audit Trail entries (NanoPhotometer booting, opening of Stored

Method and saving in the backup folder/control device)

Resolved Issues
General (affects all NanoPhotometer® models):

• Correct the folder list in the save dialog of Assistance/Selftest
• Correction for remote access in Assistance
• Handling of special characters
• New time setting sometimes does not work in Preferences
• Optimization of load mask
• Protein UV method: No dye selection if the Custom Protein factor is selected
• Protein UV method: No dye selection if OD1 factor is selected
• The result table is sometimes not displayed
• Stored Methods/Results: File directory: folder/files sometimes not displayed

CFR21 Software

• Login is not possible with a password containing some special characters

Apps for control devices

• Smart Phone: Manual Dilution is sometimes not displayed in the parameter
• Tablet: Show print icon only if printer is connected
• Tablet: Resizing of the graph zoom sometimes does not work
• Windows App: N120 sample map sometimes not opening

NPOS Version 4.3e 15670 (18.08.2021)

Implemented Functions
N/A

Improvements
Improvement of USB connection (NanoPhotometer/computer)

Resolved Issues
N/A

NPOS Version 4.3d 15670 (20.04.2021)
Implemented Functions
N/A

Improvements
N/A

Resolved Issues
General (affects all NanoPhotometer® models):
Protein UV method: Calculation of DOL

NPOS Version 4.3c 15670 (30.03.2021)
Implemented Functions

• N/A

Improvements
N/A

Resolved Issues
Concentration alerts

NPOS Version 4.3b 15670 (10.03.2021)

Implemented Functions
General (affects all NanoPhotometer® models):

• Screensaver videos
• Protein Assay and Standard Curve method: remeasurement of standard curves in the Stored

Methods

• Formula editor in the Wavelength method
• New Assistance features: Self-Test, User Manual, and Remote Access

NanoPhotometer® N120

• New multi-channel methods: Protein Assay and Wavescan
• Manual change of Sample IDs in Sample Map

CFR21 Software

• Power User can read the audit trail

Improvements

• General (affects all NanoPhotometer® models):
• New and improved warning messages for easier handling
• Protein UV method: new protein factor Custom (1/ɛ); extended (four) decimal places for Extinction Coefficient
• Protein Assay and Standard Curve method: shows by default three concentrations for curve fit, Regression 2nd Order
• Protein Assay method: default values for Baseline Correction
• Adjusted format for autosave and audit trail file names

Improvement Initialization Test

• Updated HP printer driver

NanoPhotometer® N120
Performance improvement

• Translations for multi-channel methods
• Show .csv files in Stored Methods

CFR21 Software 

• New warning message for administrator password
• Additional unique audit trail Entries

Apps for control devices

• Improved saving on smartphone/tablet: data files are saved in the Download folder
• (Android) or NanoPhotometer folder (iOS) for easy access

Resolved Issues
General (affects all NanoPhotometer® models):

• Issues with parameter settings and layout
• Workflow issues for inactive/active blank and sample button, including a warning message
• Auto Sample sometimes not working
• Clickable area not working: alert icons in the table of the Nucleic Acid / Protein UV method
• Show integer numbers for wavelength and time settings
• Keyboard randomly not shown randomly

WiFi status on home screen

• Show the correct WiFi status for connection failure
• Network Folder: issue with permissions; show the correct network status for the wrong network path
• Connection of a static IP address
• The Time Zone dropdown loads too slowly
• The cuvette port sometimes does not close
• Fix of Chinese, Japanese, and Portuguese translation issues

Disable the DNS server

• Overlay graphs are not working after opening the Assistance
• File directory: issues with white frame for folder selection; copying subfolders
• PDF files: layout issues, show correct table columns depending on method, show correct decimal places for all values
• Issues with the results %Transmission for cuvette measurements
• Protein Assay / Standard Curve method: show N/A for the factor for 2nd Order Regression standard curves (Excel / PDF), opened .ids and .json files show saved standard curve with correct values, corrupt autosave file NanoPhotometer® N120:
• Sample Map selection: change of sample loading orientation or switching between single and multi-sample clears Sample Map; selection issues for vertical mode, multiple plates, and a warning message “Position A1 must be selected”
• Sample ID setting on Sample Map: issues with vertical orientation; Sample ID setting in opened .json
• White frame issue on Sample Map/result screens after remeasuring the bad blank
• Hidden Blank: show correct Sample IDs (BLK01…); show correct warning messages on inactive sample button
• Bad blank warning: sort by Sample ID
• Multi-channel result screens: show correct number format onthe  concentration grid, correct wavelength range on the graph grid
• The single result overview is empty when swiping screens during measurement.
• Show in the graph the pop-up grid position and not consecutive numbers
• Opened .ids files with selected data saved (multi-channel method): show graphs in grid and Sample IDs in Sample Map
• Sample ID drafts (.csv) – show correct help text in .csv files for exported Sample IDs
• Show correct warning messages in case of an invalid Sample ID definition in the imported .csv file
• Do not stop measurement for imported Sample ID (.csv vertical mode and selection)
• Parameter not shown in the opened .json, navigating over the page indicator
• Bad reaction to the page indicator
• Use the correct lid factor in case of dye measurement inthe  Nucleic Acid / Protein UV multichannel method
• Layout issue in Stored Methods/Results after saving in the multichannel method
• Illuminate red LEDs correctly after stopping blank
• Optimize cryo label prints onthe  the Dymo printer for multichannel methods

Apps for control devices

Mac computer app

• Layout issues: Auto Sample button, Sample ID pop-up
• Clickable area not working: units dropdown in Nucleic Acids and Protein UV method; alert icons in result view. Saving via the Home button is not a closing method.d Windows computer app:
• Protein Assay / Standard Curve method: toggle switches for replicate selection not working, mouse scrolling issues for result view
• Protein UV multi-channel method: shows the correct value for A260/A280 in the vertical tab bar
• Phone app (iOS and Android): show the correct parameter depending on
• NanoPhotometer® model (opened method and Stored Methods)
• No back button in Stored Results/Stored Methods or opened .ids or .json
• Kinetics save on the home button not working

CFR21 Software

• Full functionality of the CFR21 software without a power user
• The audit trail folder is only accessible via the file server
• The language of the audit trail is English, independent from the language setting
• Use the Login Name and not the User ID in the audit trail
• Layout issues: selected options need to be highlighted (in method color) and greyed out if no selection is possible
• User rights: do not allow the user to change parameters in the loaded .ids files and stored
• Methods (.json); User can only measure defined Sample IDs (multi-channel method) in an open Stored Method (.json);
• Unique login and group names to avoid problems with user rights
• Show the correct file name/path of the .ids PDF and Excel document header
• The screensaver can sometimes not be deactivated / display darkened
• Adding a folder to My NanoPhotometer in the opened .ids file ends in a load mask
• Windows app: forced log off ends in load mask; dye tab in vertical tab bar sometimes not shown in the opened Stored Method
• NPOS Version 4.2h 14900 (29.06.2020)

Implemented Functions
N/A

Improvements

• Activation of SMB2 for new main PCBs

Resolved Issues
General (affects all NanoPhotometer® models):

• File Server: writing permissions on the computer; file server access problems after updating to NPOS 4. X
• Chinese: Date & Time not shown completely in PDF

NPOS Version 4.2g 14900 (31.03.2020)
Implemented Functions
N/A

Improvements
N/A

Resolved Issues
General (affects all NanoPhotometer® models):
Deactivation of screensaver videos (final approval missing)
NPOS Version 4.2f 14900 (24.01.2020)

Implemented Functions
N/A

Improvements
N/A

Resolved Issues
General (affects all NanoPhotometer® models):
Chinese / Japanese language setting: PDF saving and printing are   not working for NanoPhotometer® with the new main PCB

NPOS Version 4.2e 14900 (17.12.2019)
Implemented Functions

• N/A

Improvements
General (affects all NanoPhotometer® models):
Initialization test: no measurement with open lid

Resolved Issues
General (affects all NanoPhotometer® models):
Saving issue after save failed on a read-only USB flash drive

NPOS Version 4.2d 14900 (15.11.2019)
Implemented Functions

• N/A

Improvements
N/A

Resolved Issues
NanoPhotometer® N120:
Air Bubble Recognition of multi-channel methods is not working correctly

NPOS Version 4.2c 14900 (07.11.2019)
Implemented Functions
N/A

Improvements
N/A

Resolved Issues
General (affects all NanoPhotometer® models):
Excel of the Wavescan method: shows consistent peak position on all Excel sheets

NPOS Version 4.2b.14900 (29.10.2019)
Implemented Functions
N/A

Improvements
NanoPhotometer® N120:

Improvement ofthe  measurement head movement

Resolved Issues
CFR21 Software:

Audit trail: show consecutive IDs (no restart at 1)

NPOS Version 4.2.14900 (04.10.2019)

Implemented Functions
N/A

Improvements

• CFR21 software: second disclaimer for liability added
• Warning Messages: change of ratio alert A260/A280, Confirmation message for deleting files

Resolved Issues
General (affects all NanoPhotometer® models):

• Optimize booting time
• The keyboard sometimes does not open
• Hanging load masks: graph overlay; saving of selected data
• Allow lower and upper case extensions for .ids files
• No Auto Sample function for cuvette mode (NP80)
• Missing text “Selected Time” for time change
• File server: remember user accounts; lock button not working; writing permissions on the computer
• Network Folder: password sometimes lost; warning message for allowed characters for Network Folder Nickname
• Excel: range of x-axis for graph on values sheet
• Cryo label print on Dymo 400 and Dymo 450 Turbo is not working

NanoPhotometer® N120

• Home button not shown after tick box selection
• Layout issues for single result overview
• White frame selection on Sample Map
• Opened .ids files: display of cells without results
• Warning message for selected cells without Sample IDs
• Consistent parameter setting changing between single- and multi-channel options
• Actions for file tree in Sample ID pop-up/import .csv files: copy, delete, and rename
• Excel of single-channel Nucleic Acid / Protein UV: graph sheets are missing

CFR21 Software

• Allow lower and upper case extensions for .lic files (license file)

Software Version 4.6n build 16350
Valid from 04.10.2019 last change 27.05.2025

• Activation of the CFR21 software in a language other than English
• Opened Protein UV Stored Method shows the wrong wavelength parameter

Apps for control devices

• Layout issue on Windows computer: white square instead of x for closing a single result overview
• Concurrency issue opening .ids or .json when the control device is active
• iPhone: saving on the control device
• Android app requests to read ofthe  phone state permission
• Android apps: remove the message “Leave this page” on the control device back button when saving data on the control

28. NPOS Version 4.2.14756 (27.08.2019)
Initial release of CFR21 Software

Documents / Resources

Implen NPOS Software NanoPhotometer [pdf] User Guide 4.6n, 4.6m, 4.6L, 4.6k, 4.6j, 4.6f, NPOS Software NanoPhotometer, NPOS, Software NanoPhotometer, NanoPhotometer

References

• User Manual