Lab 20 200uL Pipettor Mai Sauyawa

GABATARWA

Sabuwar pipette hannun ku shine cikakken manufa pipette don daidaitaccen kuma madaidaicin sampling da rarraba yawan adadin ruwa. Pipettes suna aiki akan ƙa'idar motsin iska da tukwici masu zubarwa.

CODE KYAUTA	BAYANI
550.002.005	Girman 0.5 zuwa 10ul
550.002.007	2 zu20
550.002.009	10 zu100
550.002.011	20 zu200
550.002.013	100 zu1000
550.002.015	1 zu5ml

Da fatan za a karanta littafin mai amfani a hankali kafin amfani kuma bi duk umarnin aiki da aminci! Ƙayyadaddun fasaha da ƙayyadaddun bayanai suna iya canzawa ba tare da sanarwa ba.

GARANTI

Ana ba da garantin bututun na tsawon shekara guda a kan lahani a cikin kayan aiki da aikin aiki. Idan ya gaza yin aiki a kowane lokaci, da fatan za a tuntuɓi wakilin ku nan da nan. Garanti ba zai rufe lahanin lalacewa ta al'ada ba ko ta amfani da pipette a kan umarnin da aka bayar a cikin wannan jagorar.
Ana gwada kowace pipette kafin jigilar kaya ta masana'anta. Tsarin Tabbatar da inganci shine garantin ku cewa pipette ɗin da kuka siya ya shirya don amfani.
Dukkanin pipettes an gwada ingancin su bisa ga ISO8655/DIN12650. Ikon ingancin bisa ga ISO8655/DIN12650 ya haɗa da gwajin gravimetric na kowane pipette tare da ruwa mai tsafta (ingancin 3, DIN ISO 3696) a 22 ℃ ta amfani da nasihu na asali na masana'anta.

ISAR

Ana ba da wannan rukunin tare da babban naúrar 1 x, kayan aikin calibration, Tube na man shafawa, littafin mai amfani, mariƙin Pipette, Tukwici & Takaddun kulawar inganci.

BUBUWAN KARATUN MATAKI

RUWAN KARATU	KARAWA	TIPS
0.5-10 ml	0.1ml ku	10ml ku
2-20 ml	0.5 ml	200 ml
10-100 ml	1ml ku	200, 300, 350 μl
20-200 ml	1ml ku	200, 300, 350 μl
100-1000 ml	1ml ku	1000ml ku
1000-5000 ml	50ml ku	5m ku

SANAR DA MAI RIKE PIETTE

Don dacewa da aminci koyaushe kiyaye pipette a tsaye akan mariƙinsa lokacin da ba'a amfani dashi. Lokacin shigar da mariƙin, da fatan za a bi umarnin da ke ƙasa:

1. Tsaftace saman shiryayye tare da ethanol.
2. Cire takarda mai kariya daga tef ɗin m.
3. Shigar da mariƙin kamar yadda aka bayyana a ciki Hoto 2A. (Tabbatar an danna mariƙin a gefen shiryayye.)
4. Sanya pipette akan mariƙin kamar yadda aka nuna a ciki Hoto na 2B.
   
   

BAYANIN PIPETTE

AIKIN PIPETTE

Saitin ƙara 

Ana nuna ƙarar pipette a fili ta taga mai riko. Ana saita ƙarar isarwa ta hanyar juya maɓallin yatsan hannun agogo baya ko gaba da agogo (Hoto na 3). Lokacin saita ƙarar, da fatan za a tabbatar cewa:

• Ƙarar isar da ake so tana dannawa cikin wuri
• Lambobin suna bayyane gaba ɗaya a cikin taga nuni
• Ƙararren da aka zaɓa yana cikin ƙayyadadden kewayon pipette

Yin amfani da ƙarfi da yawa don kunna maɓallin turawa a waje da kewayon na iya lalata injin ɗin kuma ya lalata pipette.

Rufewa da fitar da tukwici 

• Kafin shigar da tip tabbatar cewa mazugi na pipette ya kasance mai tsabta. Danna tip akan mazugi na pipette da ƙarfi don tabbatar da hatimin hana iska. Hatimin yana matsewa lokacin da zoben hatimi na bayyane ya fito tsakanin tip da mazugi na bakin titin (Hoto na 4).
  
  

Kowane pipette an saka shi da mai fitar da tip don taimakawa kawar da haɗarin aminci da ke tattare da gurɓatawa. Ana buƙatar matsi mai fitar da tip ɗin ƙasa da ƙarfi don tabbatar da fitar da tip mai kyau (Hoto na 5). Tabbatar an jefa tip a cikin kwandon shara mai dacewa.

FASSARAR TUHU

Bututun gaba 

Tabbatar cewa tip yana haɗe sosai zuwa mazugi na tip. Don sakamako mafi kyau ya kamata a yi amfani da maɓallin yatsan yatsa a hankali kuma a hankali a kowane lokaci, musamman tare da ruwa mai ɗanɗano.

Rike pipette a tsaye yayin buri. Tabbatar cewa ruwa da jirgin ruwa suna da tsabta kuma cewa pipette, tukwici da ruwa suna cikin zafin jiki iri ɗaya.

• Matsa maɓallin yatsa zuwa tasha ta farko (Hoto.6B).
• Sanya titin a ƙarƙashin saman ruwa (2-3mm) kuma a saki maɓallin yatsa a hankali. A hankali cire tip daga cikin ruwa, taɓa gefen akwati don cire wuce haddi.
• Ana rarraba ruwa ta hanyar latsa maɓallin yatsa a hankali zuwa tasha ta farko (Hoto.6B). Bayan ɗan jinkiri ci gaba da danna maɓallin yatsa zuwa tasha ta biyu (Hoto.6C). Wannan hanya za ta zubar da tip kuma tabbatar da isarwa daidai.
• Saki maɓallin yatsa zuwa wurin da aka shirya (Hoto.6A). Idan ya cancanta canza tip kuma ci gaba da pipetting.
  

Juya bututu 

Dabarar baya ta dace don rarraba ruwa mai laushi wanda ke da halin kumfa ko yana da babban danko. Hakanan ana amfani da wannan dabarar don rarraba ƙananan juzu'i lokacin da aka ba da shawarar cewa an fara fara yin tip tare da ruwa kafin bututun. Ana samun wannan ta hanyar cikawa da zubar da tip.

1. Latsa maɓallin yatsa har zuwa tasha ta biyu (Hoto.6C). Sanya titin a ƙarƙashin saman ruwa (2-3mm) kuma a saki maɓallin yatsa a hankali.
2. Janye tip daga ruwa yana taɓa gefen akwati don cire wuce haddi.
3. Isar da ƙarar da aka saita ta hanyar danna maɓallin yatsa sannu a hankali zuwa tasha ta farko (Hoto.6B). Riƙe maɓallin yatsa a tasha ta farko. Ruwan da ya rage a cikin tip bai kamata a haɗa shi cikin bayarwa ba.
4. Ya kamata a jefar da sauran ruwa a yanzu tare da titin ko a mayar da shi cikin jirgin ruwa.

SHAWARWARIN TUHU

• Rike pipette a tsaye lokacin neman ruwa kuma sanya ƴan milimita kaɗan a cikin ruwan
• Kafin a wanke tip ɗin kafin a shayar da ruwan ta hanyar cikawa da zubar da tip sau 5. Wannan yana da mahimmanci musamman lokacin rarraba ruwa waɗanda ke da ɗanko da yawa daban da ruwa
• Koyaushe sarrafa motsin maɓallin turawa tare da babban yatsan hannu don tabbatar da daidaito
• Lokacin da ake yin bututun ruwa a yanayin zafi daban-daban da na yanayi, kafin a wanke tip ɗin sau da yawa kafin amfani.

AJIYA

Lokacin da ba a amfani da shi ana ba da shawarar cewa an adana pipette ɗinku a tsaye.

GWAJIN YI DA KYAUTA

Kowane pipette an gwada masana'anta kuma an tabbatar dashi a 22 ℃ bisa ga ISO8655/DIN12650. Tebur mai zuwa yana nuna matsakaicin kuskuren izini (Fmax) don masana'antun suna bayarwa a cikin ISO8655/DIN 12650, wanda ke ƙara ba kowane mai amfani shawara don kafa nasu matsakaicin kuskuren izini (mai amfani da Fmax). Mai amfani da Fmax kada ya wuce Fmax da fiye da 100%.

Lura: Ana ba da garantin ƙayyadaddun ƙayyadaddun Pipette kawai tare da tukwici na masana'anta.

Gwajin aiki (Checking calibration) 

• Ya kamata a yi aunawa a 20-25 ℃, akai-akai zuwa + 0.5 ℃.
• Guji zayyana.
  1. Saita ƙarar gwajin da ake so na pipette ɗinku.
  2. A hankali daidaita tip akan mazugi na tip.
  3. Pre-kurkure tip tare da distilled ruwa ta pipetting da zaɓaɓɓen girma sau 5.
  4. Yi amfani da ruwa a hankali, kiyaye pipette a tsaye.
  5. Pipette distilled ruwa a cikin kwandon kwalta ya karanta nauyin a mgs. Maimaita aƙalla sau biyar kuma yi rikodin kowane sakamako. Yi amfani da ma'aunin nazari tare da iya karantawa na 0.01 mgs. Don ƙididdige ƙarar, raba nauyin ruwa da yawa (a 20 ℃: 0.9982). Wannan hanyar ta dogara ne akan ISO8655/DIN12650.
  6. Yi ƙididdige ƙimar F ta amfani da mai zuwa

Daidaitawa: = ∣ rashin daidaito (μl) ∣+2× rashin daidaituwa (μl)
Kwatanta ƙididdigan F-darajar zuwa madaidaicin mai amfani da Fmax. Idan ya fada cikin ƙayyadaddun bayanai, pipette yana shirye don amfani. In ba haka ba duba duka daidaitonku kuma, idan ya cancanta, ci gaba zuwa tsarin gyarawa.

Hanyar sake gyarawa 

1. Sanya kayan aikin daidaitawa cikin ramukan makullin daidaitawa (ƙarƙashin maɓallin yatsa) (Hoto na 7).
   
2. Juya makullin daidaitawa kusa da agogon gaba don raguwa da agogon agogo don ƙara ƙarar.
3. Maimaita tsarin gwajin aiki (Checking calibration) daga mataki na 1 har sai sakamakon bututun ya yi daidai.

KIYAWA

Don kula da mafi kyawun sakamako daga pipette kowane ɗayan ya kamata a duba kowace rana don tsabta. Ya kamata a biya kulawa ta musamman ga mazugi (s).

An tsara pipettes don sabis na cikin gida mai sauƙi. Koyaya, muna kuma ba da cikakken sabis na gyara da daidaitawa gami da rahoton sabis da takaddun shaida(s). Da fatan za a mayar da pipette ɗin ku ga wakilin ku na gida don gyara ko gyarawa. Kafin dawowa da fatan za a tabbatar da cewa ya kuɓuta daga duk wata cuta. Da fatan za a ba da shawara ga Wakilin Sabis ɗinmu na kowane kayan haɗari waɗanda ƙila an yi amfani da su tare da pipette ɗinku.

Lura: Bincika aikin pipette akai-akai misali kowane watanni 3 kuma koyaushe bayan sabis na cikin gida ko kulawa.

Tsaftace pipette 

Don tsaftace Pipettor ɗinku, yi amfani da ethanol da yadi mai laushi ko nama mara lint. Ana bada shawara don tsaftace mazugi na tip akai-akai.

Kulawa a cikin gida 

1. Rike ƙasa mai fitar da tip.
2. Sanya haƙori na kayan aikin buɗewa tsakanin tip ejector da ƙwanƙolin ƙwanƙwasa don sakin tsarin kullewa. (Hoto na 8).
3. A hankali sakin tip ejector da kuma cire ejector kwala.
4. Sanya ƙarshen maƙarƙashiya na kayan aikin buɗewa akan mazugi na tip, juya shi gaba da agogo. Kada ku yi amfani da wasu kayan aikin (Hoto na 9). Ana cire mazugi na tip 5 ml ta hanyar juya shi gaba da agogo. Kada ku yi amfani da kowane kayan aiki (Hoto na 10).
5. Shafa fistan, O-zobe da mazugi na tip tare da ethanol da zane mara lint.
   Lura: Samfuran har zuwa 10μl suna da ƙayyadaddun O-ring da ke cikin mazugi na tip. Saboda haka, ba za a iya samun damar O-ring don kiyayewa ba.
6. Kafin musanya mazugi na tip ana ba da shawarar man shafawa piston kadan ta amfani da man siliki da aka bayar.
   Lura: Yin amfani da mai da yawa na iya matse piston.
7. Bayan sake haɗawa, yi amfani da pipette (ba tare da ruwa ba) sau da yawa don tabbatar da cewa man shafawa ya yada daidai.

Duba ma'aunin pipette.

MATSALAR HARBI

MATSALA	DALILI MAI WUYA	MAFITA
RUWAN DUMI-DUMINSU CIKI	Tukwici mara dacewa	Yi amfani da tukwici na asali
	Rikewar da ba ta dace ba na filastik	Haɗa sabon tip
TSORO KO RUWAN TUSHEN KARAMIN	Tip ba daidai ba haɗe	Haɗa da ƙarfi
	Tukwici mara dacewa	Yi amfani da tukwici na asali
	Barbashi na waje tsakanin tip da mazugi	Tsaftace mazugi na tip, haɗa sabon tip
	Kayan aiki gurɓatacce ko ƙarancin maiko akan piston da O-ring	Tsaftace da man shafawa O-ring da piston, tsaftace mazugi mai man shafawa daidai da haka
	O-ring ba a daidaita shi daidai ko lalacewa ba	Canza O-ring
	Ayyukan da ba daidai ba	Bi umarni a hankali
	Canjin daidaitawa ko bai dace da ruwa ba	Sake daidaitawa bisa ga umarnin
	Kayan aiki sun lalace	Aika don sabis
TUNTURA BUTTON YA CUTAR KO YA YI BAUTAWA	Fistan ya gurbata	Tsaftace da man shafawa O-ring da piston, tsaftace mazugi na tip
	Shiga cikin tururi mai narkewa	Tsaftace da man shafawa O-ring da piston, tsaftace mazugi na tip
AN KASHE PIPETTE RUWAN SHA'AWA MA KARAMIN	Liquid ya shiga cikin mazugi ya bushe	Tsaftace da man shafawa O-ring da piston, tsaftace mazugi na tip
TIP EJECTOR YA CUTAR DA KO YA YI BAUTAWA	Tukwici mazugi da/ko ƙwanƙolin fitar da gurɓataccen abu	Tsaftace mazugi na tip da ƙwanƙolin ejector

AUTOCKING

Ana iya haɗa pipettor gabaɗaya ta atomatik ta amfani da bakar tururi zuwa 121C na mintuna 20. Ba a buƙatar riga-kafi. Bayan an gama autoclaving, dole ne a bar pipettor don hutawa na tsawon sa'o'i 12. Ana bada shawara don duba aikin pipettor bayan kowane autoclaving. Hakanan ana ba da shawarar man shafawa piston da hatimin pipettor bayan 10 autoclavings.

GOYON BAYAN KWASTOM

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Takardu / Albarkatu

Lab 20 200uL Pipettor Mai Sauyawa [pdf] Manual mai amfani 20 200uL Mai Canjin Pipettor, 20 200uL, Mai Canjin Pipettor, Mai canzawa

Magana

• Manual mai amfani