GILSON FP10001S MypiPetman Customizable Pipette User Guide

 

Chapter 1: INTRODUCTION

MyPIPETMAN® is an air displacement pipette that uses disposable pipette tips. This pipette, depending on the version selected, is customizable to your needs while guaranteeing the Gilson quality standards. It offers both technical and personalization options for optimal use in various workflows. Lightweight, ergonomic, safe, and easy-to-use, MyPIPETMAN is a reliable, accurate, and precise pipette that provides unique and state-of-the-art features and choices:

• Simple ratchet or 3-position volume locking system including a light click and a free volume selection position
• Reduced or standard pipetting forces
• Considerably reduced or standard tip ejection forces, with a built-in ejection button integrated on top of the pipette handle
• Colors and product personalization options

To meet users’ needs, MyPIPETMAN is available in three offers, each with different levels of technical options and product customization, as described in Figure 1 below.

Figure 1
MyPIPETMAN® global offer

MyPIPETMAN is available as variable volume in either single or multichannel models.

• Eight single channel models cover a volume range from 0.2 μL to 10 mL.
• Eight multichannel models (4 models of 8 and 12 channels) cover a volume range from 0.5 μL to 300 μL.

*US web sales only

 

Parts Checklist

After unpacking the pipette, verify that the following items were included and remain undamaged:

Single Channel Models

• MyPIPETMAN pipette
• Quick Guide
• Tip ejector adapter equipped on the P2 and P10 models
• Bag of ten filters (only for P5000 and P10mL models)
• Certificate of Conformity
• Safety bag

Multichannel Models

• MyPIPETMAN pipette
• Quick Guide
• Ejector spacer for D10 tips (only for P10 models)
• Certificate of Conformity
• Safety bag

Good Laboratory Practice (GLP) Compliance
The serial number is engraved on the body of the pipette. It provides unique identification of your pipette and the manufacturing date.

Figure 2
Serial number identification example
The barcode on the box and the Certificate of Conformity provides traceability of your pipette.

Description
Please refer to the following chapters for a complete description of the different parts and functions of the pipette.

Figure 3
MYPIPETMAN® single channel and multichannel model components

Specifications
MyPIPETMAN is a high-quality pipette that has excellent accuracy and precision, especially when used with PIPETMAN® DIAMOND Tips. The data given in the “Gilson Maximum Permissible Errors” table below was obtained using PIPETMAN DIAMOND Tips. These values are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.

Each pipette is inspected and validated by qualified technicians in accordance with the Gilson quality system. Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard by type testing.

The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6).

*CV means Coefficient of Variation
NOTE The data given in this table conform to the ISO 8655-2 standard. With a precise pipetting technique, the P2 model can be used to aspirate volumes as low as 0.1 μL and the P10 model as low as 0.5 μL.

 

Chapter 2: SETTING THE VOLUME

The volume of liquid to be aspirated is set using the volume display. The dials are colored either black or red to indicate the position of the decimal point, depending on the model (refer to Figure 4 below).

Volume selection differs according to your configuration. To obtain maximum accuracy when setting the volume, proceed as follows:

• When decreasing the volume setting, slowly reach the required setting, making sure not to overshoot the volume mark.
• When increasing the volume setting, pass the required value by 1/3 of a turn and slowly decrease to reach the volume, making sure not to overshoot the volume mark.

 

 

Chapter 3: PIPETTING

 

To fit a tip ejector extension (refer to Figure 7):

1. Slide the extension over the tip holder.
2. Push the extension firmly onto the end of the tip ejector until it clicks into place.

To remove a tip ejector extension:

1. Gently twist the adapter.
2. Pull it away from the pipette.

Multichannel Models
For P8x10 and P12x10 models, according to the tip used, either D10 or DL10, you might have to fit the ejector adapter. Long collar tips can be inserted without it.

Fitting the Tips
It is recommended to use PIPETMAN DIAMOND Tips with MyPIPETMAN for optimum performance.
These tips are made from pure polypropylene. Plastic tips are for a single application and should be disposed of after use—please do not clean for reuse.

Single Channel Models
To fit a new PIPETMAN DIAMOND Tip, push the tip holder into the tip using a slight twisting motion to ensure a firm and airtight seal.

Multichannel Models
PIPETMAN DIAMOND Tips: TIPACK AND TOWERPACK
PIPETMAN DIAMOND tips are best fitted with the ROCKY RACK technique, invented by Gilson, available only in our TIPACK and TOWERPACK.

Aspirating

1. Press the push button to the first stop (this corresponds to the set volume of liquid).
2. Hold the pipette vertically and immerse the tip in the liquid (refer to Guidelines for Good Pipetting).
3. Release the push button slowly and smoothly to the top position to aspirate the set volume of liquid.
4. Wait one second (refer to Guidelines for Good Pipetting) and then withdraw the pipette tip from the liquid. You may wipe any droplets away from the outside of the tip using a medical wipe. However, if you do so, take care to avoid touching the tip’s orifice.

NOTE For the multichannel models, use a reagent reservoir.

Dispensing

1. Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).
2. Press the push button slowly and smoothly to the first stop.
3. Wait for at least a second, then press the push button to the second stop to expel any residual liquid from the tip. Keep the push button pressed fully, and while removing the pipette, draw the tip along the inside surface of the vessel.
4. Release the push button smoothly. Eject the tip by pressing firmly on the tip ejector button.

Ejecting the Tips
To avoid touching used tips, hold the pipette over the waste container and press the tip ejector push button.

NOTE

P5000 and P10mL models are not equipped with tip ejectors, and tips must be carefully removed by the user. The use of gloves is recommended for tip removal when pipetting contaminated, toxic, or corrosive liquids.

Guidelines for Good Pipetting

• Make sure that you operate the push button slowly and smoothly.
• When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table below).

 

• Change the tip before aspirating a different liquid, sample, or reagent.
• Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
• Pre-wet each new tip with the liquid to be pipetted.
• The liquid should never enter the tip holder; to prevent this:
  ○ Press and release the push button slowly and smoothly.
  ○ Never turn the pipette upside down.
  ○ Never lay the pipette on its side when there is liquid in the tip.
• If you use the same tip with a larger volume, pre-wet the tip.
• For volatile solvents, saturate the air cushion in your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample.
• When the liquid temperature is different from the ambient temperature, pre-wet the tip several times before use.
• You may remove the tip ejector (refer to Fit the Tip Ejector Adapter) to aspirate from very narrow tubes.
• After pipetting acids or other corrosive liquids that emit vapors, remove the tip ejector and tip holder, rinse with distilled water, dry, and lubricate the piston (refer to MAINTENANCE). For the P1000L model, use a specific tip holder equipped with a filter to increase the lifetime of the piston (refer to ACCESSORIES).
• Do not pipette liquids with temperatures above 70°C or below 4°C. The pipette can be used between +4°C and +40°C, but the gravimetric performance may vary according to the temperature (refer to the ISO 8655-2 standard for conditions of use).

NOTICE

MyPIPETMAN should be held in the vertical position. For detailed information about pipetting techniques, refer to the Gilson Guide to Pipetting available on the Gilson website (www.gilson.com).

 

Chapter 4: ACCESSORIES

To make pipetting more comfortable and more secure, Gilson has developed several accessories toassist in your pipetting workflows.

MyPIPETMAN Accessories for Extra Personalization
For identification, extra personalization, or more convenient use, an offer of complementary accessories is available for use with MyPIPETMAN.

 

 

Chapter 5: TROUBLESHOOTING

A quick inspection of the pipette may help you detect a potential problem.
NOTE You may download the Two Minute Inspection Poster LT800520 from the Gilson website (www.gilson.com), which shows how to perform a quick diagnosis of your pipette.

WARNING

Before returning any pipette to your local Gilson Service Center, ensure that it is completely free of chemical, biological, or radioactive contamination (refer to CLEANING AND DECONTAMINATION). Please use the included safety bag to return the pipette to your local Gilson-authorized Service Center (Click here to find your Gilson Service Center).

Troubleshooting Table
The following table may help you identify and correct the potential problem you might encounter.

NOTE

Please make sure the tips are correctly fitted. Refer to Fitting the Tips.

For single channel and multichannel models

Leak Test
This test may be performed at any time to check that the pipette does not leak, especially after performing a maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test.

For the P2 to P200 single channel models:

1. Fit a PIPETMAN DIAMOND Tip.
2. Set the pipette to the maximum volume given in the specifications and pre-wet.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds:
   ○ If a water droplet appears at the end of the tip, there is a leak.
   ○ If you see no droplets, re-immerse the tip below the surface of water. The water level inside the tip should remain constant; if the level goes down, there is a leak.

For the P1000, P5000, and P10mL single channel models:

1. Fit a PIPETMAN DIAMOND tip.
2. Set the pipette to the maximum volume given in the specifications.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds:
   ○ If a water droplet appears at the end of the tip, there is a leak.

For the multichannel models:

1. Fit the PIPETMAN DIAMOND tip.
2. Set the pipette to the maximum volume given in the specifications, and pre-wet the tips.
3. Aspirate the set volume from a reagent reservoir of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds:
   ○ If a water droplet appears at the end of the tip, there is a leak.
   ○ If you see no droplets, for volumes below 200 μL, re-immerse the tip below the surface of water.
   ○ The water level inside the tip should remain constant; there is a leak if the level goes down.

 

Chapter 6: CLEANING AND DECONTAMINATION

MyPIPETMAN pipettes are fully autoclavable without disassembly for maximum convenience as well as protection from contamination.

NOTICE
You may refer to the decontamination procedure available on www.gilson.com. Liquid must never enter any pipette’s upper part (handle).

Cleaning for Single Channel Models
The pipette must be cleaned, as described below, before it is decontaminated. Soap solution is
recommended for cleaning MyPIPETMAN.

External

1. Remove the tip ejector (refer to Changing the Tip Ejector for Single Channel Models).
2. Wipe the entire pipette and the tip ejector with a soft-cloth or lint-free tissue soaked with soap solution to remove all dirty spots or marks. If the pipette is very dirty, a brush with soft, plastic bristles may be used.
3. Wipe the entire pipette and the tip ejector with a soft cloth or lint-free tissue soaked with distilled water.
4. Refit the tip ejector and allow the pipette to dry.

Internal
Only the following components can be immersed in a cleaning solution: connecting nut, tip ejector, tip holder, piston assembly, seal, and O-ring.

1. Disassemble the pipette (refer to MAINTENANCE).
2. Set aside the upper part in a clean, dry place.
3. Clean the individual components of the lower part of the pipette using an ultrasonic bath (20 minutes at 50°C) or with a soft-cloth and brushes. Note that the piston assembly and seals must be degreased with isopropanol or ethanol before being immersed in another ultra sonic bath. Small round brushes with soft plastic bristles may be used to clean the interior of the tip holder.
4. Rinse the individual components with distilled water.
5. Leave the parts to dry by evaporation or wipe them with a clean, soft cloth or lint-free tissue.
6. Reassemble the pipette (refer to MAINTENANCE).

Cleaning for Multichannel Models
Only the following components can be immersed in a cleaning solution: tip ejector (including the ejector locks), and ejector spacer.

1. Remove the tip ejector and the ejector spacer.
2. Immerse the tip ejector and ejector spacer in the cleaning solution or wipe them with a soft cloth or lint-free tissue soaked with the cleaning solution.
3. Rinse the components with distilled water.
4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the cleaning solution.
5. Wipe it with distilled water.
6. Leave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free tissue.
7. Refit the tip ejector (refer to Changing the Tip Ejector for Multichannel Models).

 

Autoclaving
MyPIPETMAN pipettes are fully autoclavable without disassembly for maximum convenience and protection from contamination.

1. Before autoclaving
Set the pipette volume to the nominal volume of the model before placing it in the autoclave.

NOTE For pipettes equipped with the 3-position locking system, turn the selector to the left until the cursor reaches the autoclaving icon.

2. Procedure
MyPIPETMAN can be sterilized by steam autoclaving at 121°C (252°F), 1 bar relative pressure for 20 minutes without disassembly.
When autoclaving, the pipette will dry better and faster without using a bag.
3. After autoclaving
Following the autoclaving cycle, let the pipette cool down overnight to room temperature and dry thoroughly before use. Checking should be carried out according to your standard operating procedure. Leak test may also be performed to ensure that the pipette does not leak, as described in TROUBLESHOOTING, part Leak Test.

Chemical Decontamination
You may choose to decontaminate your pipette chemically in accordance with your own procedures.
Whatever decontaminant you use, check with the supplier of the decontaminant that it is compatible with stainless steel and the plastics used in the construction of the pipette: PA (Polyamide), PBT (Polybutylene Terephthalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephthalate), POM (Polyoxymethylene), PVDF (Polyvinylidene Fluoride), PP (Polypropylene), PPA (Polyphthalamide), or PPS (Polyphenylene Sulfide).

Upper Part (Handle)
1. Wipe the upper part (handle) of the pipette with a soft cloth or lint-free tissue soaked with the chosen decontaminant.
2. Wipe the upper part of the pipette with a soft cloth or lint-free tissue soaked with distilled water or sterile water.

Lower Part (Volumetric Module)
SINGLE CHANNEL MODELS
Only the following components only can be immersed in a decontaminant solution: connecting nut, tip ejector, and tip holder.

NOTE

The piston assembly and seals must be degreased with isopropanol or ethanol in a separate vessel before being immersed in decontamination solution.

1. Disassemble the pipette (refer to MAINTENANCE).
2. Immerse tip ejector, tip holder, and connecting nut in the decontamination solution.
3. Disassemble and degrease the piston assembly (Refer to Servicing Piston Assembly).
4. Rinse the connecting nut, tip ejector, and tip holder with distilled water.
5. Let the parts dry by evaporation (or wipe the tip ejector, the tip holder, and connecting nut with a soft cloth).
6. Lubricate the piston assembly and the seals (Refer to Servicing Piston Assembly).
7. Reassemble the piston assembly, the tip holder, and the tip ejector.

MULTICHANNEL MODELS
Only the following components only can be immersed in a decontamination solution: tip ejector with ejector locks, and ejector spacer.

1. Remove the tip ejector and the ejector spacer.
2. Immerse the tip ejector, ejector locks, and ejector adapter in the decontamination solution or wipe them with a soft cloth or lint-free tissue soaked with the decontamination solution.
3. Rinse the components with distilled water.
4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the decontamination solution.
5. Wipe it with distilled water.
6. Let the parts dry by evaporation or wipe them with a clean soft cloth or lint-free tissue.
   1. Refit the tip ejector (refer to Fitting the Tip Ejector Adapter).

 

Chapter 7: MAINTENANCE

Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance.

NOTICE

Gilson recommends maintenance and calibration at least annually, more frequently as needed, depending on use. The instructions provided below detail how to perform some basic maintenance yourself. Please contact your local Gilson service provider (Click here to find your local Gilson representative) to have Gilson provide maintenance and calibration for you or to ask any service-related questions.

Maintenance is limited to:

• Cleaning or decontamination (refer to CLEANING AND DECONTAMINATION)
• Replacing spare parts
• Greasing the piston assembly

Changing the Tip Ejector for Single Channel

Changing the Tip Holder for Single Channel

1. Push the ejection button.
2. Turn the tip ejector clockwise.
3. Slide and remove the tip ejector.
4. Unscrew the connecting nut by turning it clockwise.
5. Carefully separate the lower and upper parts.
6. Reassemble the pipette with the changed tip holder.
7. Tighten the connecting nut (turn counterclockwise)

NOTICE

Removing the tip holder could impact pipette performance. Performance should be checked upon refitting the new tip holder. Extra care should be taken with the piston for the P2/P10 volumes, improper installation can cause critical damage to the piston. Users should also keep track of seals/O-rings and make sure they are reinstalled properly. Failure to reinstall correctly can result in a leaking pipette.

Changing the Tip Ejector for Multichannel Models

Servicing the Piston Assembly
You may remove the piston assembly for cleaning purposes only. If the piston assembly is changed, the pipette must be adjusted and calibrated at an authorized Gilson Service Center.

 

Chapter 8: SPARE PARTS

The parts listed below include MyPIPETMAN accessories, also mentioned in chapter 4 – ACCESSORIES for extra personalization.

 

 

NOTICE

For pipette service and maintenance, or for extra spare parts, please contact your local Gilson Service provider.

 

Chapter 9: WARRANTY

Gilson warrants this pipette against defects in material under normal use and service for a period of three years from the date of purchase.

This warranty shall not apply to pipettes which are subject to abnormal use and/or improper or inadequate maintenance (contrary to the recommendations given in the user’s guide), including, but not limited to pipettes which have been subjected to physical damage, improper handling, spillage or exposure to any corrosive environment, abuse misuse or misapplication, or from ordinary wear and tear (including seal and O-rings). This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes.

Routine cleaning, control, and recalibration are not covered under the warranty. The replacement of wearing parts such as seals, O-rings, broken pistons assembly, and broken tip holders are not covered under the warranty.

NOTICE Yearly routine maintenance is highly recommended to keep your pipette in good condition, ensuring a continued high level of performance.

THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.

Specifications subject to change without notification — errors excepted.

www.gilson.com/contactus
LT801622/C | ©2022 Gilson, Inc. All rights reserved.

 

Read More About This Manual & Download PDF:

Documents / Resources

GILSON FP10001S MypiPetman Customizable Pipette [pdf] User Guide P2, FP10001S, FP10001S MypiPetman Customizable Pipette, MypiPetman Customizable Pipette, Customizable Pipette, Pipette

References

• User Manual