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User Guide for GILSON models including: P2, FP10001S, FP10001S MypiPetman Customizable Pipette, MypiPetman Customizable Pipette, Customizable Pipette, Pipette
MyPIPETMAN is an air displacement pipette that uses disposable pipette tips. This pipette, depending on the version selected, is customizable to your needs ...
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DocumentDocumentUser's Guide EN FULLY UTO CLAVABL E A TABLE OF CONTENTS TABLE OF CONTENTS INTRODUCTION | 3 Parts Checklist | 4 GLP Features | 4 Description | 4 Specifications | 5 SETTING THE VOLUME | 6 Simple Ratchet | 6 3-Position Locking System | 6 PIPETTING | 7 Fitting the Tip Ejector Adapter | 7 Fitting the Tips | 8 Pre-Wetting the Tips | 8 Aspirating | 9 Dispensing | 9 Ejecting the Tips | 9 Guidelines for Good Pipetting | 10 ACCESSORIES | 11 MyPIPETMAN Accessories for Extra Personalization | 11 TROUBLESHOOTING | 14 Troubleshooting Table | 14 Leak Test | 15 CLEANING AND DECONTAMINATION | 15 Cleaning for Single Channel Models | 15 Cleaning for Multichannel Models | 16 Autoclaving | 16 Chemical Decontamination | 17 MAINTENANCE | 18 Changing the Tip Ejector for Single Channel | 18 Changing the Tip Holder for Single Channel | 18 Changing the Tip Ejector for Multichannel | 18 Servicing the Piston Assembly | 19 SPARE PARTS | 20 WARRANTY | 21 2 | MyPIPETMAN® INTRODUCTION Chapter 1 INTRODUCTION MyPIPETMAN® is an air displacement pipette that uses disposable pipette tips. This pipette, depending on the version selected, is customizable to your needs while guaranteeing the Gilson quality standards. It offers both technical and personalization options for optimal use in various workflows. Lightweight, ergonomic, safe, and easy-to-use, MyPIPETMAN is a reliable, accurate, and precise pipette that provides unique and state-of-the-art features and choices: Simple ratchet or 3-position volume locking system including a light click and a free volume selection position Reduced or standard pipetting forces Considerably reduced or standard tip ejection forces, with a built-in ejection button integrated on top of the pipette handle Colors and product personalization options To meet users' needs, MyPIPETMAN is available in three offers, each with different levels of technical options and product customization, as described in Figure 1 below. MyPIPETMAN® Select Volume Locking Technical Options Available Pipetting Forces Ejection Forces Tip Ejectors (Single Channel, P2 to P1000 Models) Pipette Colors Customization Options Special Text Printing Special Logo Printing 3-Position Locking System Simple Rachet Reduced Standard Reduced Standard Metal Plastic Ordering Information Minimum Order Quantity Available Countries Possible on Request 1 Worldwide MyPIPETMAN® Enterprise Possible on Request (Subject to Specific Conditions) 150 Units (Mix of Different Models) Worldwide MyPIPETMAN® 1 US Web Sales Only Figure 1 MyPIPETMAN® global offer MyPIPETMAN is available as variable volume in either single or multichannel models. Eight single channel models cover a volume range from 0.2 L to 10 mL. Eight multichannel models (4 models of 8 and 12 channels) cover a volume range from 0.5 L to 300 L. Model MyPIPETMAN® Select MyPIPETMAN® Single Channel P2 FP10001S P10 FP10002S P20 FP10003S P100 FP10004S P200 FP10005S P1000 FP10006S P5000 P10mL FP10007S FP10008S MyPIPETMAN® Multichannel P8x10 FP10009S P12x10 FP10010S P8x20 FP10011S P12x20 FP10012S P8x200 P12x200 FP10013S FP10014S P8x300 FP10015S P12x300 FP10016S MYPIPETMAN SERIES PART NUMBERS MyPIPETMAN® Select Text Printing On Demand MyPIPETMAN® Enterprise MyPIPETMAN® * FP10001SP FP10002SP FP10003SP FP10004SP FP10005SP FP10006SP FP10007SP FP10008SP FP10009SP FP10010SP FP10011SP FP10012SP FP10013SP FP10014SP FP10015SP FP10016SP FP10001E FP10002E FP10003E FP10004E FP10005E FP10006E FP10007E FP10008E FP10009E FP10010E FP10011E FP10012E FP10013E FP10014E FP10015E FP10016E FP10001 FP10002 FP10003 FP10004 FP10005 FP10006 FP10007 FP10008 FP10009 FP10010 FP10011 FP10012 FP10013 FP10014 FP10015 FP10016 *US web sales only MyPIPETMAN® | USER'S GUIDE 3 INTRODUCTION Parts Checklist After unpacking the pipette, verify that the following items were included and remain undamaged: Single Channel Models Multichannel Models MyPIPETMAN pipette Quick Guide Tip ejector adapter equipped on the P2 and P10 models Bag of ten filters (only for P5000 and P10mL models) Certificate of Conformity Safety bag MyPIPETMAN pipette Quick Guide Ejector spacer for D10 tips (only for P10 models) Certificate of Conformity Safety bag Good Laboratory Practice (GLP) Compliance The serial number is engraved on the body of the pipette. It provides unique identification of your pipette and the manufacturing date. MyPIPETMAN Select and MyPIPETMAN Enterprise serial number type: A A 50001 Year Month Production number YEAR 2022 2023 2024 2025 2026 2027 CODE T U W X Y Z MONTH January February March April May June July CODE A B C D E G H MyPIPETMAN serial number type: August J MY 524 A A September K October L Model Random Month Year Number November M December N Figure 2 Serial number identification example The barcode on the box and the Certificate of Conformity provides traceability of your pipette. Description Please refer to the following chapters for a complete description of the different parts and functions of the pipette. Single channel Multichannel Color-coded push button Locking system Tip ejector button Colored window Customizable handle Color ring Connecting nut Ejector clip Ejector lock Cover Tip holder Tip ejector Figure 3 MYPIPETMAN® single channel and multichannel model components 4 INTRODUCTION | MyPIPETMAN® INTRODUCTION Specifications MyPIPETMAN is a high-quality pipette that has excellent accuracy and precision, especially when used with PIPETMAN® DIAMOND Tips. The data given in the "Gilson Maximum Permissible Errors" table below was obtained using PIPETMAN DIAMOND Tips. These values are guaranteed only when genuine PIPETMAN DIAMOND Tips are used. Each pipette is inspected and validated by qualified technicians in accordance with the Gilson quality system. Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard by type testing. The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6). Model PIPETMAN® DIAMOND Tips MYPIPETMAN® SINGLE CHANNEL MODELS Maximum Permissible Errors Vol. (µL) Systematic Error (µL) Gilson ISO 8655 Random Error (µL) Systematic Random Error Error (%) (%CV*) Systematic Random Error Error (µL) (µL) Systematic Random Error Error (%) (%CV*) MyPIPETMAN D10 P2 DL10 0.2 ± 0.024 0.012 ± 12.0 6.0 ± 0.08 0.04 ± 40.0 20.0 0.5 ± 0.025 0.012 ± 5.0 2.4 ± 0.08 0.04 ± 16.0 8.0 1 ± 0.027 0.013 ± 2.7 1.3 ± 0.08 0.04 ± 8.0 4.0 2 ± 0.030 0.014 ± 1.5 0.7 ± 0.08 0.04 ± 4.0 2.0 MyPIPETMAN D10 P10 DL10 1 ± 0.025 0.012 ± 2.5 1.2 ± 0.12 0.08 ± 12.0 8.0 5 ± 0.075 0.030 ± 1.5 0.6 ± 0.12 0.08 ± 2.4 1.6 10 ± 0.100 0.040 ± 1.0 0.4 ± 0.12 0.08 ± 1.2 0.8 MyPIPETMAN P20 D200 2 ± 0.10 0.030 ± 5.0 1.5 ± 0.2 0.1 ± 10.0 5.0 10 ± 0.10 0.050 ± 1.0 0.5 ± 0.2 0.1 ± 2.0 1.0 20 ± 0.20 0.060 ± 1.0 0.3 ± 0.2 0.1 ± 1.0 0.5 MyPIPETMAN P100 D200 10 ± 0.35 0.10 ± 3.5 1.0 ± 0.8 0.3 ± 8.0 3.0 50 ± 0.40 0.12 ± 0.8 0.24 ± 0.8 0.3 ± 1.6 0.6 100 ± 0.80 0.15 ± 0.8 0.15 ± 0.8 0.3 ± 0.8 0.3 MyPIPETMAN P200 D200 20 100 200 ± 0.50 ± 0.80 ± 1.60 0.20 0.25 0.30 ± 2.5 ± 0.8 ± 0.8 1.0 0.25 0.15 ± 1.6 ± 1.6 ± 1.6 0.6 0.6 0.6 ± 8.0 ± 1.6 ± 0.8 3.0 0.6 0.3 MyPIPETMAN P1000 D1000 100 500 1000 ± 3.0 ± 4.0 ± 8.0 0.6 1.0 1.5 ± 3.0 ± 0.8 ± 0.8 0.6 0.2 0.15 ± 8.0 ± 8.0 ± 8.0 3.0 3.0 3.0 ± 8.0 ± 1.6 ± 0.8 3.0 0.6 0.3 MyPIPETMAN P5000 D5000 500 2500 5000 ± 12 ± 15 ± 30 3 5 8 ± 2.4 ± 0.6 ± 0.6 0.6 0.2 0.16 ± 40 ± 40 ± 40 15.0 15.0 15.0 ± 8.0 ± 1.6 ± 0.8 3.0 0.6 0.3 MyPIPETMAN P10mL D10mL 1000 5000 10000 ± 30 ± 40 ± 60 6 ± 3.0 0.6 ± 60 30.0 ± 6.0 3.0 10 ± 0.8 0.2 ± 60 30.0 ± 1.2 0.6 16 ± 0.6 0.16 ± 60 30.0 ± 0.6 0.3 Model PIPETMAN® DIAMOND Tips MyPIPETMAN P8x10 MyPIPETMAN P12x10 MyPIPETMAN P8x20 MyPIPETMAN P12x20 MyPIPETMAN P8x200 MyPIPETMAN P12x200 MyPIPETMAN P8x300 MyPIPETMAN P12x300 D10 DL10 DL10 D200 D200 D300 MYPIPETMAN® MULTICHANNEL MODELS Vol. (µL) Maximum Permissible Errors Gilson ISO 8655 Systematic Random Systematic Random Systematic Random error error error error error error (µL) (µL) (%) (%CV*) (µL) (µL) Systematic Random error error (%) (%CV*) 0.5 ± 0.08 0.04 ± 16.0 8.0 ± 0.24 0.16 ± 48.0 32.0 1 ± 0.08 0.05 ± 8.0 5.0 ± 0.24 0.16 ± 24.0 16.0 5 ± 0.20 0.10 ± 4.0 2.0 ± 0.24 0.16 ± 4.8 3.2 10 ± 0.20 0.10 ± 2.0 1.0 ± 0.24 0.16 ± 2.4 1.6 2 ± 0.10 0.08 ± 5.0 4.0 ± 0.4 0.2 ± 20.0 10.0 10 ± 0.20 0.10 ± 2.0 1.0 ± 0.4 0.2 ± 4.0 2.0 20 ± 0.40 0.15 ± 2.0 0.75 ± 0.4 0.2 ± 2.0 1.0 20 100 200 20 30 150 300 ± 0.50 ± 1.00 ± 2.00 ± 1.00 ± 1.00 ± 1.50 ± 3.00 0.25 0.40 0.50 ± 2.5 ± 1.0 ± 1.0 0.35 0.35 0.60 1.00 ± 5.0 ± 3.3 ± 1.0 ± 1.0 1.25 0.4 0.25 ± 3.2 ± 3.2 ± 3.2 1.75 1.17 0.4 0.33 ± 8.0 ± 8.0 ± 8.0 ± 8.0 1.2 1.2 1.2 3.0 3.0 3.0 3.0 ± 16.0 ± 3.2 ± 1.6 ± 40.0 ± 26.67 ± 5.33 ± 2.67 6.0 1.2 0.6 15.0 10.0 2.0 1.0 *CV means Coefficient of Variation NOTE The data given in this table conform to the ISO 8655-2 standard. With a precise pipetting technique, the P2 model can be used to aspirate volumes as low as 0.1 L and the P10 model as low as 0.5 L. MyPIPETMAN® | USER'S GUIDE 5 SETTING THE VOLUME Chapter 2 SETTING THE VOLUME The volume of liquid to be aspirated is set using the volume display. The dials are colored either black or red to indicate the position of the decimal point, depending on the model (refer to Figure 4 below). Volume selection differs according to your configuration. To obtain maximum accuracy when setting the volume, proceed as follows: When decreasing the volume setting, slowly reach the required setting, making sure not to overshoot the volume mark. When increasing the volume setting, pass the required value by 1/3 of a turn and slowly decrease to reach the volume, making sure not to overshoot the volume mark. MODEL COLOR OF VOLUMETER NUMBERS BLACK RED INCREMENT MyPIPETMAN SINGLE CHANNEL MyPIPETMAN P2 µL 0.01 µL MyPIPETMAN P10 µL 0.1 µL MyPIPETMAN P20 µL 0.1 µL MyPIPETMAN P100 MyPIPETMAN P200 MyPIPETMAN P1000 MyPIPETMAN P5000 MyPIPETMAN P10mL µL µL 0.01 mL 0.01 mL mL mL mL 0.1 mL MyPIPETMAN MULTICHANNEL MyPIPETMAN P8x10 µL MyPIPETMAN P12x10 µL MyPIPETMAN P8x200 µL MyPIPETMAN P12x200 µL MyPIPETMAN P8x300 µL MyPIPETMAN P12x300 µL 0.1 µL 0.1 µL - 0.002 µL 0.02 µL 0.02 µL 0.2 µL 0.2 µL 0.002 mL 0.002 mL 0.02 mL 0.02 µL 0.02 µL 0.2 µL 0.2 µL 0.2 µL 0.2 µL Single channel model P2 P10 P20 P100 1 0 1 0 2 7 2 7 5 5 5 5 1.25 µL 7.5 µL 12.5 µL 75 µL P200 1 2 5 P1000 0 7 5 P5000 1 2 5 P10mL 0 7 5 125 µL 0.75 mL 1.25 mL 7.5 mL Figure 4 Dial colors by model Multichannel model x10 x20 0 1 7 2 5 5 7.5 µL 12.5 µL x200 1 2 5 x300 1 2 5 125 µL 125 µL Simple Ratchet For pipettes mounted with a simple ratchet, select the volume by turning the push button (refer to Figure 5). 3-Position Locking System For pipettes equipped with a 3-position locking system, turn the selector to your desired position (refer to Figure 6). Free volume selection position 1. Turn the selector to the left until the cursor reaches the unlocking 1 . 2. Select the volume by turning the push button. Light click volume selection position 1. Turn the selector until the cursor reaches the middle 2 . 2. Select the volume by turning the push button. NOTE When selecting the volume, you will hear click sounds, allowing for more precise settings. Use these sounds for final fine volume selection. Secure Pipetting After selecting your volume, turn the selector to the right until the cursor reaches the locking 3 for secure pipetting. Figure 5 Simple ratchet Figure 6 3-position locking system NOTE To ensure good performance, always lock the pipette before pipetting by selecting the locking icon. 6 SETTING THE VOLUME | MyPIPETMAN® 1 2 3 PIPETTING Chapter 3 PIPETTING A Fitting the Tip Ejector Adapter Single Channel Models A For P2 and P10 pipettes equipped with a stainless-steel tip ejector, a dual-position adapter is required to fit and eject DL10 tips (long tips) and D10 tips (short tips). To fit the adapter for short and long tips (refer to Figure 7): 1. Pull the adapter down from the stainless steel tip ejector. 180° 2. Turn the adapter 180°. 3. Refit the adapter so that the end of the stainless steel tip ejector engages the shorter or longer slot of the adapter. Short collar Long collar tips tips B To fit To remove B For P2 and P10 pipettes equipped with a plastic tip ejector, a dual-position adapter is required to fit and eject D10 tips (short tips). Figure 7 Tip ejector dual position adapter and extension for P2L and P10L To fit a tip ejector extension (refer to Figure 7): 1. Slide the extension over the tip holder. 2. Push the extension firmly onto the end of the tip ejector until it clicks into place. To remove a tip ejector extension: 1. Gently twist the adapter. 2. Pull it away from the pipette. Multichannel Models For P8x10 and P12x10 models, according to the tip used, either D10 or DL10, you might have to fit the ejector adapter. Long collar tips can be inserted without it. 1. Remove the tip ejector, keep both ejector locks depressed 1 , and then pull the tip ejector down 2 . 2. Fit the ejector adapter 3 and click it to the tip ejector. 3. To refit the tip ejector, gently re-insert the tip ejector vertically into the rails of the ejector support 4 . NOTE Long tips can be used without tip ejector adapter. 1 2 3 4 MyPIPETMAN® | USER'S GUIDE 7 PIPETTING Fitting the Tips It is recommended to use PIPETMAN DIAMOND Tips with MyPIPETMAN for optimum performance. These tips are made from pure polypropylene. Plastic tips are for a single application and should be disposed of after use--please do not clean for reuse. Single Channel Models To fit a new PIPETMAN DIAMOND Tip, push the tip holder into the tip using a slight twisting motion to ensure a firm and airtight seal. PIPETMAN DIAMOND Tip Compatibility for Single Channel P2, P10 D10, DL10, DF10ST, DFL10ST P20 D200, DF30ST P100 D200, DF100ST P200 D200, D300, DF200ST, DF300ST P1000 D1000, D1200, DF1000ST, DF1200ST P5000 D5000 P10mL D10mL NOTE D5000 and D10mL PIPETMAN DIAMOND Tips do not have any filter. P5000 and P10mL models are sold with a bag of 10 filters. Bags of filters are also available as accessories. Refer to ACCESSORIES page. The filters, made of polyethylene, offer the same properties as the filters which are inside the PIPETMAN DIAMOND filter tips. They maintain the pipette's original accuracy and precision by creating an efficient barrier to aerosols or corrosive vapors such as acids which could damage mechanical parts. Insert the filter directly in the pipette tip holder. Change the filter every day or more frequently depending on the intensity of use and/or concentration of the solution. Filters are not autoclavable and must be disposed of after use. Multichannel Models PIPETMAN DIAMOND Tips: TIPACK AND TOWERPACK PIPETMAN DIAMOND tips are best fitted with the ROCKY RACK technique, invented by Gilson, available only in our TIPACK and TOWERPACK. PIPETMAN DIAMOND Tip Compatibility for Multichannel Models P8x10, P12x10 D10*, DL10, DF10ST, DFL10ST P8x20, P12x20 DL10, D200, DFL10ST, DF30ST P8x200, P12x200 D200, D300, DF200ST, DF300ST P8x300, P12x300 D200, D300, DF200ST, DF300ST *Using an ejector adapter, you can adapt a D10 tip (refer to page 7). Pre-Wetting the Tips Pre-wetting the tips before pipetting helps prepare the tips for the best pipetting performance. Ideally, the pre-wet includes both immersing the tip in the liquid and performing one pipetting step. Pre-wetting the tips helps ensure that volumes that you pipette will achieve accuracy and precision within specifications. Figure 8 ROCKY RACK technique 8 PIPETTING | MyPIPETMAN® PIPETTING Aspirating 1. Press the push button to the first stop (this corresponds to the set volume of liquid). 2. Hold the pipette vertically and immerse the tip in the liquid (refer to Guidelines for Good Pipetting). 3. Release the push button slowly and smoothly to the top position to aspirate the set volume of liquid. 4. Wait one second (refer to Guidelines for Good Pipetting) and then withdraw the pipette tip from the liquid. You may wipe any droplets away from the outside of the tip using a medical wipe. However, if you do so, take care to avoid touching the tip's orifice. NOTE For the multichannel models, use a reagent reservoir. Dispensing 1. Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°). 2. Press the push button slowly and smoothly to the first stop. 3. Wait for at least a second, then press the push button to the second stop to expel any residual liquid from the tip. Keep the push button pressed fully, and while removing the pipette, draw the tip along the inside surface of the vessel. 4. Release the push button smoothly. Eject the tip by pressing firmly on the tip ejector button. Prepare Aspirate Dispense Purge Home Rest Position First stop Second stop Figure 9 Pipetting motion - aspirate and dispense Ejecting the Tips To avoid touching used tips, hold the pipette over the waste container and press the tip ejector push button. NOTE P5000 and P10mL models are not equipped with tip ejectors, and tips must be carefully removed by the user. The use of gloves is recommended for tip removal when pipetting contaminated, toxic, or corrosive liquids. MyPIPETMAN® | USER'S GUIDE 9 PIPETTING Guidelines for Good Pipetting Make sure that you operate the push button slowly and smoothly. When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table below). Model P2, P10, P8x10, P12x10 P20, P8x20, P12x20, P8x200, P12x200 P8x300, P12x300 P1000 P5000 P10mL Immersion Depth (Millimeters) 1 2-3 2-4 2-4 3-6 5-7 Wait Time (Seconds) 1 1 1 2-3 4-5 4-5 Change the tip before aspirating a different liquid, sample, or reagent. Change the tip if a droplet remains at the end of the tip from the previous pipetting operation. Pre-wet each new tip with the liquid to be pipetted. The liquid should never enter the tip holder; to prevent this: Press and release the push button slowly and smoothly. Never turn the pipette upside down. Never lay the pipette on its side when there is liquid in the tip. If you use the same tip with a larger volume, pre-wet the tip. For volatile solvents, saturate the air cushion in your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample. When the liquid temperature is different from the ambient temperature, pre-wet the tip several times before use. You may remove the tip ejector (refer to Fit the Tip Ejector Adapter) to aspirate from very narrow tubes. After pipetting acids or other corrosive liquids that emit vapors, remove the tip ejector and tip holder, rinse with distilled water, dry, and lubricate the piston (refer to MAINTENANCE). For the P1000L model, use a specific tip holder equipped with a filter to increase the lifetime of the piston (refer to ACCESSORIES). Do not pipette liquids with temperatures above 70°C or below 4°C. The pipette can be used between +4°C and +40°C, but the gravimetric performance may vary according to the temperature (refer to the ISO 8655-2 standard for conditions of use). NOTICE MyPIPETMAN should be held in the vertical position. For detailed information about pipetting techniques, refer to the Gilson Guide to Pipetting available on the Gilson website (www.gilson.com). 10 PIPETTING | MyPIPETMAN® ACCESSORIES Chapter 4 ACCESSORIES To make pipetting more comfortable and more secure, Gilson has developed several accessories to assist in your pipetting workflows. CARROUSELTM TRIOTM Description CARROUSEL pipette Stand (7 pipettes) TRIO Pipette Stand (3 pipettes) Universal Multichannel Stand* SINGLE Pipette Holder Part Number F161401 F161405 F161417 F161406 Universal Multichannel Stand SINGLETM * Universal multichannel stand is for multichannel models only. Disposable Reagent Reservoirs are ideal for dispensing reagent with multichannel pipettes. Description Part Number Reagent reservoir 25 mL, x100 F267660 Reagent reservoir 50 mL, x100 F267670 With the JIMMYTM Microtube Opener, a hands-free microtube opener, you can open both snap-cap and screw-cap microtubes. Description Part Number JIMMY Microtube Opener (set of 3) F144983 For P5000 and P10mL models, disposable filters maintain the pipette's original accuracy and precision by creating an efficient barrier to aerosols or corrosive vapours such as acids which could damage mechanical parts. Description Bag of 10 Filters for P5000 /P10mL Bag of 100 Filters for P5000 / P10mL Part Number F161280 F161241 To protect the piston when pipetting corrosive liquids, you can use a specific tip holder and filter for the model P1000. Description Part Number Corrosion Protection Kit (tip holder + a bag of 10 filters) Corrosion Protection Kit Filter (Bag of 10)* F144570 F144571 Corrosion Protection Kit Filter (Bag of 50)* F144572 *Filters can be inserted in the specific tip holder provided in the Corrosion Protection Kit (part Number F144570) MyPIPETMAN® | USER'S GUIDE 11 ACCESSORIES MyPIPETMAN Accessories for Extra Personalization For identification, extra personalization, or more convenient use, an offer of complementary accessories is available for use with MyPIPETMAN. COLORED WINDOWS Model Window 3digit Dark Blue MyPIPETMAN Window 3digit White MyPIPETMAN Window 3digit Blue MyPIPETMAN Window 3digit Red MyPIPETMAN Window 3digit Yellow MyPIPETMAN Window 3digit Green MyPIPETMAN Part Number FP070101 FP070102 FP070103 FP070104 FP070105 FP070106 Dark blue White Blue Red Yellow Green METAL TIP EJECTORS Model Metal Ejector Dark Blue P2/P10 MyPIPETMAN Metal Ejector White P2/P10 MyPIPETMAN Metal Ejector Dark Blue P20 MyPIPETMAN Metal Ejector White P20 MyPIPETMAN Metal Ejector Dark Blue P100 MyPIPETMAN Metal Ejector White P100 MyPIPETMAN Metal Ejector Dark Blue P200 MyPIPETMAN Metal Ejector White P200 MyPIPETMAN Metal Ejector Dark Blue P1000 MyPIPETMAN Metal Ejector White P1000 MyPIPETMAN Part Number FP070111 FP070112 FP070121 FP070122 FP070131 FP070132 FP070141 FP070142 FP070151 FP070152 Dark blue stainless steel tip ejector White stainless steel tip ejector PLASTIC TIP EJECTORS Model Plastic Ejector Dark Blue P2/P10 MYPIPETMAN Plastic Ejector White P2/P10 MYPIPETMAN Plastic Ejector Dark Blue P20 MYPIPETMAN Plastic Ejector White P20 MYPIPETMAN Plastic Ejector Dark Blue P100 MYPIPETMAN Plastic Ejector White P100 MYPIPETMAN Plastic Ejector Dark Blue P200 MYPIPETMAN Plastic Ejector White P200 MYPIPETMAN Plastic Ejector Dark Blue P1000 MYPIPETMAN Plastic Ejector White P1000 MYPIPETMAN Part Number FP070161 FP070162 FP070171 FP070172 FP070181 FP070182 FP070191 FP070192 FP070201 FP070202 EJECTOR ADAPTORS Model Metal Ejector Adaptor P2/P10 Translucent Metal Ejector Adaptor P2/P10 White Plastic Ejector Adaptor P2/P10 White Part Number FP070210 FP070212 FP070222 Dark blue plastic tip ejector White plastic tip ejector Translucent adaptor for metal ejector White adaptor for metal ejector Adaptor for plastic ejector 12 ACCESSORIES | MyPIPETMAN® ACCESSORIES TIP HOLDERS Model Tip Holder Dark Blue P2 MyPIPETMAN Tip Holder White P2 MyPIPETMAN Tip Holder Dark Blue P10 MyPIPETMAN Tip Holder White P10 MyPIPETMAN Tip Holder Dark Blue P20 MyPIPETMAN Tip Holder White P20 MyPIPETMAN Tip Holder Dark Blue P100 MyPIPETMAN Tip Holder White P100 MyPIPETMAN Tip Holder Dark Blue P200 MyPIPETMAN Tip Holder White P200 MyPIPETMAN Tip Holder Dark Blue P1000 MyPIPETMAN Tip Hholder White P1000 MyPIPETMAN Tip Holder Dark Blue P5000 MyPIPETMAN Tip Holder White P5000 MyPIPETMAN Tip Holder Dark Blue P10mL MyPIPETMAN Tip Holder White P10mL MyPIPETMAN Part Number FP070231 FP070232 FP070241 FP070242 FP070251 FP070252 FP070261 FP070262 FP070271 FP070272 FP070281 FP070282 FP070291 FP070292 FP070301 FP070302 PLASTIC EJECTORS Model Plastic Ejector 8X Dark Blue MyPIPETMAN Plastic Ejector 8X White MyPIPETMAN Plastic Ejector 12X Dark Blue MyPIPETMAN Plastic Ejector 12X White MyPIPETMAN Part Number FP070311 FP070312 FP070321 FP070322 EJECTOR ADAPTERS Model Ejector Adapter 8X White Ejector Adapter 12X White Part Number FG07064 FG07065 Dark blue tip holder White tip holder Dark blue plastic tip ejector White plastic tip ejector Ejector adapter MyPIPETMAN® | USER'S GUIDE 13 TROUBLESHOOTING Chapter 5 TROUBLESHOOTING A quick inspection of the pipette may help you detect a potential problem. NOTE You may download the Two Minute Inspection Poster LT800520 from the Gilson website (www.gilson.com), which shows how to perform a quick diagnosis of your pipette. WARNING Before returning any pipette to your local Gilson Service Center, ensure that it is completely free of chemical, biological, or radioactive contamination (refer to CLEANING AND DECONTAMINATION). Please use the included safety bag to return the pipette to your local Gilson-authorized Service Center (Click here to find your Gilson Service Center). Troubleshooting Table The following table may help you identify and correct the potential problem you might encounter. NOTE Please make sure the tips are correctly fitted. Refer to Fitting the Tips. For single channel and multichannel models Symptom Pipette is leaking sample Pipette will not aspirate Pipetted volume is inaccurate Pipette is not precise Possible Cause Damaged tip holder Worn O-ring or seal Worn O-ring Damaged tip holder Connecting nut is loose Damaged or corroded piston Improper repair or assembly Improper repair or assembly Unscrew tip holder Connecting nut is loose Tip holder is loose Connecting nut is loose Incorrect operator technique Damaged or corroded piston(s) Damaged tip holder(s) Worn O-ring or seal Low quality tips Damaged tip holder(s) Pipetting seize up S = Single Channel M = Multichannel Damaged tip ejector adapter Damaged ejector Dirty tip holder The tip ejector is loose The ejector lock is misaligned Piston needs lubricant Action Replace the tip holder Replace both parts and lubricate Replace O-Ring and seal and lubricate Replace the tip holder Tighten connecting nut Return pipette to supplier Refer to MAINTENANCE Refer to MAINTENANCE Tighten connecting nut Tighten connecting nut Tighten connecting nut Tighten connecting nut Operator training Return pipette to supplier Replace the tip holder(s) Replace both parts and lubricate Use PIPETMAN DIAMOND Tips Replace the tip holder(s) for single channel model. Contact your local Gilson Service Center for multichannel model Damaged tip ejector adapter Replace the tip ejector Clean the tip holder with isopropanol or ethanol Assemble the tip ejector properly Align the ejector lock Lubricate piston assembly NOTE If you cannot solve the problem, contact your local Gilson-authorized Service Center. Model* S S S S S+M S S S S S+M S S+M S+M S S S S+M S+M S+M S+M S+M S S S 14 TROUBLESHOOTING | MyPIPETMAN® CLEANING AND DECONTAMINATION Leak Test This test may be performed at any time to check that the pipette does not leak, especially after performing a maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test. For the P2 to P200 single channel models: 1. Fit a PIPETMAN DIAMOND Tip. 2. Set the pipette to the maximum volume given in the specifications and pre-wet. 3. Aspirate the set volume from a beaker of distilled water. 4. Maintain the pipette in the vertical position and wait for 20 seconds: If a water droplet appears at the end of the tip, there is a leak. If you see no droplets, re-immerse the tip below the surface of water. The water level inside the tip should remain constant; if the level goes down, there is a leak. For the P1000, P5000, and P10mL single channel models: 1. Fit a PIPETMAN DIAMOND tip. 2. Set the pipette to the maximum volume given in the specifications. 3. Aspirate the set volume from a beaker of distilled water. 4. Maintain the pipette in the vertical position and wait for 20 seconds: If a water droplet appears at the end of the tip, there is a leak. For the multichannel models: 1. Fit the PIPETMAN DIAMOND tip. 2. Set the pipette to the maximum volume given in the specifications, and pre-wet the tips. 3. Aspirate the set volume from a reagent reservoir of distilled water. 4. Maintain the pipette in the vertical position and wait for 20 seconds: no leak If a water droplet appears at the end of the tip, there is a leak. If you see no droplets, for volumes below 200 µL, re-immerse the tip below the surface of water. The water level inside the tip should remain constant; there is a leak if the level goes down. Figure 10 MyPIPETMAN® multichannel leak test Chapter 6 CLEANING AND DECONTAMINATION MyPIPETMAN pipettes are fully autoclavable without disassembly for maximum convenience as well as protection from contamination. NOTICE You may refer to the decontamination procedure available on www.gilson.com. Liquid must never enter any pipette's upper part (handle). Cleaning for Single Channel Models The pipette must be cleaned, as described below, before it is decontaminated. Soap solution is recommended for cleaning MyPIPETMAN. External 1. Remove the tip ejector (refer to Changing the Tip Ejector for Single Channel Models). 2. Wipe the entire pipette and the tip ejector with a soft-cloth or lint-free tissue soaked with soap solution to remove all dirty spots or marks. If the pipette is very dirty, a brush with soft, plastic bristles may be used. 3. Wipe the entire pipette and the tip ejector with a soft cloth or lint-free tissue soaked with distilled water. 4. Refit the tip ejector and allow the pipette to dry. MyPIPETMAN® | USER'S GUIDE 15 CLEANING AND DECONTAMINATION Internal Only the following components can be immersed in a cleaning solution: connecting nut, tip ejector, tip holder, piston assembly, seal, and O-ring. 1. Disassemble the pipette (refer to MAINTENANCE). 2. Set aside the upper part in a clean, dry place. 3. Clean the individual components of the lower part of the pipette using an ultrasonic bath (20 minutes at 50°C) or with a soft-cloth and brushes. Note that the piston assembly and seals must be degreased with isopropanol or ethanol before being immersed in another ultra sonic bath. Small round brushes with soft plastic bristles may be used to clean the interior of the tip holder. 4. Rinse the individual components with distilled water. 5. Leave the parts to dry by evaporation or wipe them with a clean, soft cloth or lint-free tissue. 6. Reassemble the pipette (refer to MAINTENANCE). Cleaning for Multichannel Models Only the following components can be immersed in a cleaning solution: tip ejector (including the ejector locks), and ejector spacer. 1. Remove the tip ejector and the ejector spacer. 2. Immerse the tip ejector and ejector spacer in the cleaning solution or wipe them with a soft cloth or lint-free tissue soaked with the cleaning solution. 3. Rinse the components with distilled water. 4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the cleaning solution. 5. Wipe it with distilled water. 6. Leave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free tissue. 7. Refit the tip ejector (refer to Changing the Tip Ejector for Multichannel Models). Autoclaving MyPIPETMAN pipettes are fully autoclavable without disassembly for maximum convenience and protection from contamination. 1. Before autoclaving Set the pipette volume to the nominal volume of the model before placing it in the autoclave. NOTE For pipettes equipped with the 3-position locking system, turn the selector to the left until the cursor reaches the autoclaving icon. 2. Procedure MyPIPETMAN can be sterilized by steam autoclaving at 121°C (252°F), 1 bar relative pressure for 20 minutes without disassembly. When autoclaving, the pipette will dry better and faster without using a bag. 3. After autoclaving Following the autoclaving cycle, let the pipette cool down overnight to room temperature and dry thoroughly before use. Checking should be carried out according to your standard operating procedure. Leak test may also be performed to ensure that the pipette does not leak, as described in TROUBLESHOOTING, part Leak Test. 16 CLEANING AND DECONTAMINATION | MyPIPETMAN® CLEANING AND DECONTAMINATION Chemical Decontamination You may choose to decontaminate your pipette chemically in accordance with your own procedures. Whatever decontaminant you use, check with the supplier of the decontaminant that it is compatible with stainless steel and the plastics used in the construction of the pipette: PA (Polyamide), PBT (Polybutylene Terephthalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephthalate), POM (Polyoxymethylene), PVDF (Polyvinylidene Fluoride), PP (Polypropylene), PPA (Polyphthalamide), or PPS (Polyphenylene Sulfide). Upper Part (Handle) 1. Wipe the upper part (handle) of the pipette with a soft cloth or lint-free tissue soaked with the chosen decontaminant. 2. Wipe the upper part of the pipette with a soft cloth or lint-free tissue soaked with distilled water or sterile water. Lower Part (Volumetric Module) SINGLE CHANNEL MODELS Only the following components only can be immersed in a decontaminant solution: connecting nut, tip ejector, and tip holder. NOTE The piston assembly and seals must be degreased with isopropanol or ethanol in a separate vessel before being immersed in decontamination solution. 1. Disassemble the pipette (refer to MAINTENANCE). 2. Immerse tip ejector, tip holder, and connecting nut in the decontamination solution. 3. Disassemble and degrease the piston assembly (Refer to Servicing Piston Assembly). 4. Rinse the connecting nut, tip ejector, and tip holder with distilled water. 5. Let the parts dry by evaporation (or wipe the tip ejector, the tip holder, and connecting nut with a soft cloth). 6. Lubricate the piston assembly and the seals (Refer to Servicing Piston Assembly). 7. Reassemble the piston assembly, the tip holder, and the tip ejector. MULTICHANNEL MODELS Only the following components only can be immersed in a decontamination solution: tip ejector with ejector locks, and ejector spacer. 1. Remove the tip ejector and the ejector spacer. 2. Immerse the tip ejector, ejector locks, and ejector adapter in the decontamination solution or wipe them with a soft cloth or lint-free tissue soaked with the decontamination solution. 3. Rinse the components with distilled water. 4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the decontamination solution. 5. Wipe it with distilled water. 6. Let the parts dry by evaporation or wipe them with a clean soft cloth or lint-free tissue. 1. Refit the tip ejector (refer to Fitting the Tip Ejector Adapter). MyPIPETMAN® | USER'S GUIDE 17 MAINTENANCE Chapter 7 MAINTENANCE Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance. NOTICE Gilson recommends maintenance and calibration at least annually, more frequently as needed, depending on use. The instructions provided below detail how to perform some basic maintenance yourself. Please contact your local Gilson service provider (Click here to find your local Gilson representative) to have Gilson provide maintenance and calibration for you or to ask any service-related questions. Maintenance is limited to: Cleaning or decontamination (refer to CLEANING AND DECONTAMINATION) Replacing spare parts Greasing the piston assembly Changing the Tip Ejector for Single Channel To remove 1. Push the ejection button 1 . 2. Turn the tip ejector clockwise 2 . 3. Slide and remove the tip ejector 3 . To refit 1. Push the ejection button. 2. Slide the tip ejector along the tip holder. 3. Clip the tip ejector on the body of the pipette. 1 2 3 Changing the Tip Holder for Single Channel 1. Push the ejection button. 2. Turn the tip ejector clockwise. 3. Slide and remove the tip ejector. 4. Unscrew the connecting nut by turning it clockwise. 5. Carefully separate the lower and upper parts. 6. Reassemble the pipette with the changed tip holder. 7. Tighten the connecting nut (turn counterclockwise) NOTICE Removing the tip holder could impact pipette performance. Performance should be checked upon refitting the new tip holder. Extra care should be taken with the piston for the P2/P10 volumes, improper installation can cause critical damage to the piston. Users should also keep track of seals/O-rings and make sure they are reinstalled properly. Failure to reinstall correctly can result in a leaking pipette. Changing the Tip Ejector for Multichannel Models To remove 1. Keep both ejector locks depressed 1 . 2. Pull the tip ejector down 2 . To refit 1. Gently re-insert the tip ejector vertically into the rails of the ejector support 3 . 2. Pull lightly on the tip ejector to check the position. 1 2 3 18 MAINTENANCE | MyPIPETMAN® Color-coded push buton Locking system 1 Tip ejector button MAINTENANCE P10, P20, P100, P200 Connecting nut 4 2 P1000 P5000 P10mL Piston assembly 2 3 Tip holder Tip ejector Figure 11 MyPIPETMAN® piston assembly (disassembled) Servicing the Piston Assembly You may remove the piston assembly for cleaning purposes only. If the piston assembly is changed, the pipette must be adjusted and calibrated at an authorized Gilson Service Center. NOTE As models P2 and P10 contain miniaturized parts, it is best not to disassemble these pipettes yourself. Separate the upper part and the lower part 1. Push the ejection button 1 . 2. Turn the tip ejector clockwise 2 . 3. Slide and remove the tip ejector 3 . 4. Unscrew the connecting nut 4 . Carefully separate the tip holder from the upper part (refer to Figure 11) 1. Remove the piston assembly, O-ring, and the seals (refer to Changing the Tip Ejector for Single Channel). 2. Leave the piston exposed, clean it with isopropanol or ethanol, and lubricate lightly (lubricant P/N: 5440011070). 3. Clean, autoclave, or replace the tip holder. If necessary, lightly lubricate the piston and its seals. Figure 12 Lubricate the internal part of the piston For P20, P100, P200, models: lubricate only the useful part of the piston (25 ±/-5 mm length) and the O-ring (refer to Figure 12). NOTE For P1000 model: lubricate the piston. For P5000, P10mL models: disassemble the seals, lubricate the internal part of the seal, and then lubricate the useful part of the piston. Do not lubricate the O-ring. 4. Reassemble the pipette. 5. Tighten the connecting nut (turn counterclockwise). 6. Refit the tip ejector NOTE If the piston assembly is changed, the pipette must be adjusted and calibrated in at an authorized Gilson Service Center. MyPIPETMAN® | USER'S GUIDE 19 SPARE PARTS Chapter 8 SPARE PARTS The parts listed below include MyPIPETMAN accessories, also mentioned in chapter 4 ACCESSORIES for extra personalization. MyPIPETMAN SINGLE CHANNEL SPARE PARTS Model Seal+ O-ring (5 sets) Adpator for Metal Ejector Adapter for Plastic Ejector White Stainless Steel Tip Ejector Dark Blue Stainless Steel Tip Ejector White Dark blue White Plastic Tip Plastic Tip Ejector Tip Ejector Holder Dark Blue Tip Holder P2 F144861 FP070212 FP070210* FP070222 FP070112 FP070111 FP070162 FP070161 FP070232 FP070231 P10 FA07012 FP070212 FP070210* FP070222 FP070112 FP070111 FP070162 FP070161 FP070242 FP070241 P20 FA07013 - - FP070122 FP070121 FP070172 FP070171 FP070252 FP070251 P100 FA07014 - - FP070132 FP070131 FP070182 FP070181 FP070262 FP070261 P200 FA07015 - - FP070142 FP070141 FP070192 FP070191 FP070272 FP070271 P1000 FA07016 - - FP070152 FP070151 FP070202 FP070201 FP070282 FP070281 P5000 FA07307 - - - - - - FP070292 FP070291 P10mL FA07308 - - - - - - FP070302 FP070301 *Translucent Adapter MyPIPETMAN MULTICHANNEL SPARE PARTS MODEL DARK BLUE PLASTIC TIP EJECTOR WHITE PLASTIC TIP EJECTOR MyPIPETMAN P8x10 FP070311 FP070312 MyPIPETMAN P12x10 FP070321 FP070322 MyPIPETMAN P8x20 FP070311 FP070312 MyPIPETMAN P12x20 FP070321 FP070322 MyPIPETMAN P8x200 FP070311 FP070312 MyPIPETMAN P12x200 FP070321 FP070322 MyPIPETMAN P8x300 FP070311 FP070312 MyPIPETMAN P12x300 FP070321 FP070322 EJECTOR ADAPTER FG07064 FG07065 - SPARE PARTS FOR ALL MODELS MODEL PART NUMBER Window 3digit Dark Blue MyPIPETMAN FP070101 Window 3digit White MyPIPETMAN FP070102 Window 3digit Blue MyPIPETMAN FP070103 Window 3digit Red MyPIPETMAN FP070104 Window 3digit Yellow MyPIPETMAN FP070105 Window 3digit Green MyPIPETMAN FP070106 Lubricant Tube for PIPETMAN (1g) 5440011070 NOTICE For pipette service and maintenance, or for extra spare parts, please contact your local Gilson Service provider. 20 SPARE PARTS | MyPIPETMAN® WARRANTY Chapter 9 WARRANTY Gilson warrants this pipette against defects in material under normal use and service for a period of three years from the date of purchase. This warranty shall not apply to pipettes which are subject to abnormal use and/or improper or inadequate maintenance (contrary to the recommendations given in the user's guide), including, but not limited to pipettes which have been subjected to physical damage, improper handling, spillage or exposure to any corrosive environment, abuse misuse or misapplication, or from ordinary wear and tear (including seal and O-rings). This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates. Gilson's sole liability under this warranty shall be limited to, at Gilson's sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes. Routine cleaning, control, and recalibration are not covered under the warranty. The replacement of wearing parts such as seals, O-rings, broken pistons assembly, and broken tip holders are not covered under the warranty. NOTICE Yearly routine maintenance is highly recommended to keep your pipette in good condition, ensuring a continued high level of performance. THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR IMPLIED WARRANTY. MyPIPETMAN® | USER'S GUIDE 21 Specifications subject to change without notification -- errors excepted. www.gilson.com/contactus LT801622/CD | ©2022 Gilson, Inc. All rights reserved.