dynamic BIOSENSORS HK-EA-2 RNA Enzyme Activity Kit
Key Features
- ssRNA template (48 + 32 bp) for functionalization of heliX
- Adapter Chip on Spot 1.
- dsDNA-RNA template (48 + 32 bp) for functionalization of heliX
- Adapter Chip on Spot 2.
- Compatible with heliX Adapter Chip.
- Includes Adapter strands for 100 regenerations.
- This RNA template carries a moderately hydrophilic red dye (Ra) with a single positive net charge.
Helix Adapter Chip Overview
- 2 spots with 2 different anchor sequences for RNA-encoded addressing.
Product Description
Order Number: HK-EA-2
Table 1. Contents and Storage Information
For research use only.
This product has a limited shelf life, please see the expiry date on the label. To avoid many freeze-thaw cycles please aliquot the nanolever.
Preparation
IMPORTANT
Both Adapter strands are already pre-hybridized. Adapter strand 1 to the Template-32-PRa RNA strand, leaving the upper part as ssDNA, and cTemplate-Adapter strand 2 to the Template-32-PRa RNA strand, leaving Spot 2 completely as dsDNA-RNA. Next, simply mix in the same vial the Adapter strand 1 – nf / Template-32-P-Ra RNA (200/250 nM) and the template Adapter strand 2 – nf / Template-32-P-Ra RNA (200/250 nM) at a 1:1 ratio (v/v). The solution is ready to use for biochip functionalization.
Useful Order Numbers
Table 2. Order Numbers
Assay Setup in heliOS
For studying enzymatic activity of a nucleic acid modifying enzyme (e.g., polymerase, reverse transcriptase, helicase, etc.). Go to heliOS > create a New Assay Workflow > add Custom Assay > load Enzyme Binding and Activity > modify the parameters based on your needs and run the assay. Suggested assay parameters (e.g., flow rates, functionalization time, LED power, etc.) are within the heliOS assay.
TIP
Is strongly recommended to perform binding kinetics of the enzyme beforehand. The obtained Kd during enzyme kinetics can be the initial test concentration for the association of the enzyme during enzyme kinetics. This concentration is a good compromise to not overcrowd the surface and avoid multiple enzymes binding to the same template.
TIP
For an initial scouting of the substrate, choose a broad concentration splitting spanning the low nanomolar to high micromolar, and a blank. A minimum of 6 concentrations of the substrate are recommended to obtain a reliable sigmoidal fit during the extraction of the KM. For inhibition assay, analysis, or any further questions, please contact the support team at support@dynamicbiosensors.com.
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
Documents / Resources
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dynamic BIOSENSORS HK-EA-2 RNA Enzyme Activity Kit [pdf] User Manual v1.1, HK-EA-2 RNA Enzyme Activity Kit, HK-EA-2, RNA Enzyme Activity Kit, Enzyme Activity Kit, Activity Kit, Kit |
