heliX +
User Manual
ADAPTER STRAND PACKAGE
with red dye Ra
Dynamic Biosensors GmbH & Inc.
ASP-1-Ra v5.1
Key Features
- Adapter strand 1 – Ra and Adapter strand 2 – Ra – lfs (ligand free strand) for functionalization of heliX® Adapter Chip on Spot 1 and Spot 2, respectively.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strands 1 and 2 carry a moderately hydrophilic red dye (Ra) with a single positive net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: ASP-1-Ra
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
| Adapter strand 1 – Ra | Black | 400 nM | 5 x 100 µL | TE40 [1] | -20°C |
| Adapter strand 2 – Ra – lfs | White | 200/250 nM | 5 x 200 µL | TE40 [1] | -20°C |
For research use only.
This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nanolever.
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 2 and Adapter strand 2 – Ra – lfs (200 nM) at 1:1 ratio (v/v). Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
| Ligand strand carrying the conjugated ligand | 500 nM | PE40 [2] | heliX® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Required volume for 3 functionalizations: 100 µL with a final concentration of 100 nM.
| Vial 1 | Vial 2 | |
| Adapter strand 1 – Ra (400 nM) | Conjugated Ligand strand (500 nM) | Adapter strand 2 – Ra – lfs (200/250 nM) |
| 25 µL | 25 µL | 50 µL |
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
Contact
| Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany |
Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 Woburn, MA 01801 USA |
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
[2] If the protein is not stable in PE40 (TE40, HE40),
please check buffer compatibility with the switchSENSE® compatibility sheet.
ASP-1-Ra v5.1
www.dynamic-biosensors.com
Documents / Resources
 |
dynamic BIOSENSORS ASP-1-Ra Adapter Strand Package [pdf] User Manual ASP-1-Ra, ASP-1-Ra v5.1, ASP-1-Ra Adapter Strand Package, ASP-1-Ra, Adapter Strand Package, Strand Package |
