dynamic BIOSENSORS AS-2-Ga-lfs v5.1 Adapter Strand
Product Information
Specifications
- Product Name: heliX+
- Model: ADAPTER STRAND 2 with green dye Ga
- Prehybridized with ligand strand
- Manufacturer: Dynamic Biosensors GmbH & Inc.
- Order Number: AS-2-Ga-lfs v5.1
- Key Features: 2 spots with 2 different anchor sequences for DNA-encoded addressing
Product Description
The heliX+ ADAPTER STRAND 2 is a product prehybridized with a ligand strand and features 2 spots with 2 different anchor sequences for DNA-encoded addressing. It comes with green dye Ga for easy visualization.
Contents and Storage Information:
- Material: Adapter strand 2 – Ga – lfs
- Cap Color: White
- For research use only. Limited shelf life, check expiry date on label
- To avoid freeze-thaw cycles, please aliquot the nanolever
- Buffer: TE40
Product Usage Instructions
Preparation | MIX&RUN
- Mix Adapter strand 1 – Ga (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution from step 1 at room temperature at 600 rpm for 30 minutes for complete hybridization.
- Mix the solution from step 2 with Adapter strand 2 – Ga – lfs (200 nM) at 1:1 ratio (v/v).
- The solution is now ready for use in biochip functionalization.
Note: The stability of the solution is dependent on the ligand molecule’s stability.
FAQ
- Q: Can the heliX+ ADAPTER STRAND 2 be used for multiple experiments?
- A: The product is designed for single-use experiments due to its limited shelf life and stability concerns.
- Q: How should I store the heliX+ ADAPTER STRAND 2?
- A: Store the product according to the storage information provided in the manual, and avoid exposing it to frequent freeze-thaw cycles.
Key Features
- Adapter strand 2 for functionalization of heliX® Adapter Chip Spot 2.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 2 carries a hydrophilic green dye (Ga) with a single negative net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing
Product Description
Order Number: AS-2-Ga-lfs
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
| Adapter strand 2 – Ga – lfs | White | 200/250 nM | 5 x 200 µL | TE40 [1] | -20°C |
For research use only.
This product has a limited shelf life, please see expiry date on the label. To avoid many freeze-thaw cycles please aliquot the nano level
Preparation
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Ga (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 2 and Adapter strand 2 – Ga – lfs (200 nM) at 1:1 ratio (v/v). Solution is ready to use for biochip functionalization. Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
| Adapter strand 1 – Ga | 400 nM | TE40 [1] | Adapter strand 1 with green dye Ga | AS-1-Ga |
| Ligand strand
carrying the conjugated ligand |
500 nM | P2] E40 [ | heliX® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Required volume for 3 functionalizations: 100 μL with a final concentration of 100 nM.
| Vial 1 | Vial 2 | |
| Adapter strand 1 – Ga
(400 nM) |
Conjugated Ligand strand
(500 nM) |
Adapter strand 2 – Ga – lfs
(200/250 nM) |
| 25 µL | 25 µL | 50 µL |
After incubation time, mix vial 1 and vial 2 to obtain 100 μL of ready-to-use DNA solution
Contact
Dynamic Biosensors GmbH
- Perchtinger Str. 8/10
- 81379 Munich
- Germany
Dynamic Biosensors, Inc.
- 300 Trade Center, Suite 1400
- Woburn, MA 01801
- USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
- TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
- If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet
Documents / Resources
 |
dynamic BIOSENSORS AS-2-Ga-lfs v5.1 Adapter Strand [pdf] User Manual AS-2-Ga-lfs v5.1, AS-2-Ga-lfs v5.1 Adapter Strand, Adapter Strand, Strand |
