BRUKER AS-2-Rb Spark Optical Emission Spectrometer User Manual
Key Features
- Adapter strand 2 for function alization of heliX ® Adapter Chip Spot 2.
- Compatible with heliX ® Adapter Chip.
- Includes Adapter strands for 50 regeneration.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 2 carries a hydrophilic red dye (Rb) with a neutral net charge
heliX ® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-2-Rb
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
| Adapter strand 2 – Rb | White | 400 nM | 5 x 100 µL | TE40 [1] | -20°C |
For research use only.
This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nano lever.
Preparation | MIX&RUN
In-solution hybridisation of adapter and ligand strands:
- Mix Adapter strand 1 – Rb (400 nM) and conjugated Ligand strand with ligand 1 (500 nM) at 1:1 ratio (v/v).
- Mix Adapter strand 2 – Rb (400 nM) and conjugated Ligand strand with ligand 2 (500 nM) at 1:1 ratio (v/v).
- Incubate separately the two solutions of step 1 and 2 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 1 and 2 at 1:1 ratio (v/v).
Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecules.
Table 2. Additional material for function alization of spot 1 and reference spot 2
| Material | Concentration | Buffer | Related Product Name | Order No |
| Adapter strand 1 – Rb | 400 nM | TE40 [1] | Adapter strand 2 with red dye Rb | AS-1-Rb |
| Ligand strand carrying the conjugated ligand 1 |
500 nM | PE40 [2] | heliX® Amine Coupling Kit 1 | HK-NHS-1 |
| Ligand strand carrying the conjugated ligand 2 |
500 nM | PE40 [2] | heliX® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Required volume for 3 function alizations: 100 µL with a final concentration of 100 nM
| Vial 1 | Vial 2 | ||
| Adapter strand 1 – Rb(400 nM) | Conjugated Ligand strand with ligand 1 (500 nM) | Adapter strand 2 – Rb(400 nM) | Conjugated Ligand strand + with ligand 2 (500 nM) |
| 25 µL | 25 µL | 25 µL | 25 µL |
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
Contact
Dynamic Biosensors GmbH
Interchange Str. 8/10
81379 Munich
Germany
Bruker Scientific LLC
40 Manning Road, Manning Park
Billerica, MA 01821
USA
Order Information: order@dynamic-biosensors.com
Technical Support: support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2025 Dynamic Biosensors GmbH
For Research Use Only. Not for use in clinical diagnostic procedures.
- TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween 20, 50 μM EDTA, 50 μM EGTA
- If the protein is not stable in PE40 (TE40, HE 40), please check buffer compatibility with the switchSENSE® compatibility sheet.
AS-2-Rb v5.1
www.dynamic-biosensors.com
Documents / Resources
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BRUKER AS-2-Rb Spark Optical Emission Spectrometer [pdf] User Manual AS-2-Rb v5.1, AS-2-Rb Spark Optical Emission Spectrometer, AS-2-Rb, Spark Optical Emission Spectrometer, Emission Spectrometer, Spectrometer |
