CGOLDENWALL 16L Cryogenic Container Liquid Nitrogen Tank YDS-15 User Manual

1. Product Overview

The CGOLDENWALL YDS-15 Cryogenic Container is a high-performance liquid nitrogen (LN2) storage tank designed for long-term, low-temperature preservation of biological samples, medical materials, and other sensitive items. Constructed from durable aluminum alloy, it features advanced vacuum insulation to ensure extended holding times and minimal LN2 evaporation. This manual provides essential information for the safe and effective use of your cryogenic container.

Figure 1: CGOLDENWALL Cryogenic Containers for Low Temperature Storage. These tanks are designed for long-term storage with vacuum insulation.

2. Safety Information

WARNING: Liquid nitrogen is an extremely cold substance (-196°C / -320°F) and can cause severe cold burns or frostbite upon contact with skin or eyes. Always handle with extreme caution.

• Personal Protective Equipment (PPE): Always wear appropriate cryogenic gloves, a face shield or safety glasses, and long-sleeved clothing when handling liquid nitrogen or working near the container.
• Ventilation: Use the container only in well-ventilated areas. Liquid nitrogen rapidly vaporizes into gaseous nitrogen, which can displace oxygen and lead to asphyxiation in confined spaces.
• Pressure Buildup: Never seal the container completely. The lid must allow for the escape of gaseous nitrogen to prevent dangerous pressure buildup. Ensure the stopper is not airtight.
• Spills: In case of a liquid nitrogen spill, evacuate the area immediately and allow the nitrogen to evaporate. Ensure adequate ventilation before re-entering.
• Material Compatibility: Do not place materials directly into liquid nitrogen that are not designed for cryogenic temperatures, as they may become brittle and shatter.
• Lifting: Use proper lifting techniques or equipment when moving a filled container due to its weight.

3. Product Components

The CGOLDENWALL cryogenic container is designed with several key components to ensure efficient and safe storage. Understanding these parts is essential for proper operation and maintenance.

Figure 2: Internal Structure of a Cryogenic Container (Storage Type). Key components include the outer shell, vacuum insulation, inner liner, neck tube, and canisters for sample storage.

• Outer Shell: The external casing, typically made of aluminum alloy, providing structural integrity.
• Inner Liner: The internal vessel that holds the liquid nitrogen and samples.
• Vacuum Insulation: The space between the outer shell and inner liner is evacuated to create a vacuum, significantly reducing heat transfer and minimizing LN2 evaporation.
• Multilayer Insulation: Additional reflective layers within the vacuum space to further reduce radiant heat transfer.
• Neck Tube: A narrow tube connecting the inner liner to the outside, minimizing heat entry while allowing access.
• Cover/Lid: Seals the neck tube opening, often with a loose fit to prevent pressure buildup.
• Handle: For carrying the container.
• Canisters (Dewars): Small, cylindrical containers placed inside the tank to hold samples. The YDS-15 model includes 3 canisters.
• Adsorbent: Material used to maintain the vacuum over time.

4. Technical Specifications

Figure 3: Range of CGOLDENWALL Cryogenic Container Models. The YDS-15 is part of this series, offering a 16L capacity.

5. Setup Instructions

1. Unpacking: Carefully remove the cryogenic container from its packaging. Inspect for any signs of damage during transit. If damage is found, contact your supplier immediately.
2. Placement: Place the container on a stable, level surface in a well-ventilated area, away from direct sunlight or heat sources. Ensure there is sufficient space around the container for safe operation and ventilation.
3. Initial Cleaning: Before first use, ensure the interior of the container and canisters are clean and dry. Use a clean, lint-free cloth. Do not use abrasive cleaners.
4. Pre-cooling (Optional but Recommended): For optimal performance and to minimize initial LN2 boil-off, it is recommended to pre-cool the container by adding a small amount of liquid nitrogen (e.g., 1-2 liters) and allowing it to sit for several hours or overnight before filling to capacity. This helps to cool the inner vessel gradually.
5. Canister Insertion: Place the empty canisters into the container's neck tube. Ensure they are properly seated.

6. Operating Instructions

6.1 Filling with Liquid Nitrogen

1. Safety First: Ensure all safety precautions (Section 2) are strictly followed. Wear appropriate PPE.
2. Remove Lid: Carefully remove the container's lid.
3. Transfer LN2: Slowly pour liquid nitrogen from a suitable transfer vessel into the cryogenic container. Avoid splashing. Fill to the desired level, typically leaving some headspace to prevent spillage and ensure proper venting.
4. Observe Boil-off: Initial filling will result in significant boil-off (visible vapor). This is normal as the container cools down. Reduce the pouring rate if boil-off is excessive.
5. Replace Lid: Once filled, replace the lid loosely. Do not seal tightly. The lid is designed to allow vapor to escape.

6.2 Storing Samples

1. Prepare Samples: Ensure samples are properly sealed in cryovials or other suitable cryogenic storage containers. Label all samples clearly.
2. Load Canisters: Place the prepared samples into the designated slots within the canisters.
3. Lower Canisters: Carefully lower the loaded canisters into the liquid nitrogen tank using the provided handle or retrieval tool. Avoid sudden movements that could cause splashing.
4. Record Keeping: Maintain a detailed inventory of all stored samples, including their location within the canisters and tank.

6.3 Retrieving Samples

1. Safety First: Wear appropriate PPE.
2. Locate Sample: Refer to your inventory to identify the correct canister and sample location.
3. Retrieve Canister: Carefully lift the desired canister using the retrieval tool. Minimize the time the canister is exposed to ambient air to prevent warming and excessive LN2 loss.
4. Remove Sample: Quickly remove the required sample from the canister.
5. Return Canister: Immediately lower the canister back into the liquid nitrogen.
6. Replace Lid: Ensure the lid is replaced loosely after access.

7. Maintenance

• Regular LN2 Level Checks: Monitor the liquid nitrogen level regularly (e.g., weekly or bi-weekly, depending on usage and holding time). Do not allow the LN2 level to drop below the top of the stored samples.
• Refilling: Refill the tank with liquid nitrogen as needed to maintain optimal storage conditions.
• Cleaning: Periodically, when the tank is empty, clean the interior and exterior surfaces with a mild detergent and water. Rinse thoroughly and ensure all parts are completely dry before refilling. Do not use harsh chemicals or abrasive materials.
• Vacuum Integrity Check: A significant increase in LN2 evaporation rate indicates a potential loss of vacuum integrity. If this occurs, the container may need professional inspection or replacement.
• Lid and Stopper: Ensure the lid and stopper are clean and free from ice buildup. Replace if damaged.

8. Troubleshooting

9. Typical Applications

The CGOLDENWALL cryogenic container is versatile and suitable for a wide range of applications requiring ultra-low temperature storage or controlled cooling processes.

• Medical and Biological Research: Preservation of cells, tissues, vaccines, bacterial strains, and other biological samples.
• Livestock Industry: Long-term frozen storage of fine livestock semen and embryos.
• Medical Beauty Industry: Applications such as cryotherapy for treating common skin conditions.
• Industrial Cooling: Metal cooling assembly and other processes requiring extreme cold.

Figure 4: Application in Livestock Industry.

Figure 5: Application in Medical Beauty Industry.

Figure 6: Application in Medical Research.

10. Warranty and Support

For warranty information, technical support, or service inquiries regarding your CGOLDENWALL YDS-15 Cryogenic Container, please contact your authorized dealer or the manufacturer directly. Please have your product model number (YDS-15) and purchase date available when contacting support.

Manufacturer: CGOLDENWALL

For further assistance, you may visit the CGOLDENWALL Store on Amazon.