3M™ Attest™ Auto-reader 490 System
Quick Reference Guide
VH2O2 BI PCD use
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| Remove Remove 1295PCD from pouch. Remove only the number needed. Leave remaining in pouch. |
Confirm and verify Confirm process indicator is unprocessed. Verify media ampoule is intact. |
Place DO NOT open prior to sterilization. Place in sterilizer; top rack, towards front of chamber. |
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| Don After cycle completion, don safety glasses and gloves. |
Verify Verify 1348 CI is ACCEPT. Verify media ampoule is intact. |
Remove, verify, identify Remove 1295 BI from pack. Verify process indicator is exposed. Identify 1295 BI by writing on label. |
STEAM and VH2O2 BI activation and incubation
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| Crush Squeeze BI in activator to close cap and crush media ampoule. |
Flick Remove BI and flick wrist to ensure media flows to growth chamber. |
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| Inspect Verify media has transferred to BI growth chamber at the vial bottom. |
Incubate Place activated BI in any well. Display shows remaining minutes of incubation or test results. DO NOT REMOVE BI until the (+) or (-) symbol indicates the test is complete. |
NOTES:
- Activate and incubate 1295 BI within 1 hour of completion of the VH2O2 sterilization cycle
- Gloves are recommended when activating 1295 BI
- Allow 1491 and 1492V BIs to cool 10 minutes prior to activation
Interpretation of Solventum sterilization assurance core products
| STEAM Indicator | Unexposed | Acceptable Exposed |
| 3M™ Attest™ BI 1491 and 1492V |  |
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| After processing, indicator on cap turns light brown or darker. | ||
| 3M™ Comply™ Indicator Tape 1322 |  |
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| After processing, indicator stripes turn dark-brown/black. | ||
| 3M™ Attest™ Indicator Tape 1355 |  |
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| After processing, indicator stripes turn dark-brown/black. | ||
| VH2O2 Indicator | Unexposed | Acceptable Exposed Color Range |
| 3M™ Attest™ BI 1295 |  |
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| After processing, indicator stripes turn toward pink. | ||
| 3M™ Comply™ Indicator Tape 1228 |   |
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| After processing, indicator stripes turn toward pink. | ||
| 3M™ Attest™ Tri-Metric Chemical Indicator 1348/1348E |  |
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| After processing, a color change towards pink should have entered the ACCEPT window. | ||
| STEAM Indicator | Unprocessed | Acceptable Processed PASS | Unacceptable Processed FAIL | ||
| 3M™ Attest™ Steam Chemical Integrator 1243 |  |
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| After processing, indicator bar crosses into the ACCEPT region. If the color bar is in the REJECT region or on the line, it is considered a fail. | |||||
| 3M™ Comply™ Bowie-Dick Plus Test Pack 00135LF 3M™ Comply™ Early Warning Test Sheet (above) 3M™ Comply™ Bowie-Dick Test Sheet (below) |  |
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| When indicator ink circle on Early Warning Test sheet is lighter than the color standard, this may indicate that sterilizer maintenance should be scheduled. | |||||
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| After processing, a uniform color change to dark brown/black is a pass. Fail result is indicated by a non-uniform color development,with a lighter colored area, usually near the center. | |||||
| STEAM Indicator | Unprocessed | Processed | Processed PASS | Processed FAIL |
| 3M™ Attest™ eBowie-Dick Test System 3M™ Attest™ eBowie-Dick Test Card (left) 3M™ Attest™ eBowie-Dick Auto-reader (right) |
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| After processing, indicator ink circle on test card darkens. | After processing, a light-up green check mark (✓) is a pass. | After processing, a light-up red X is a fail. |
© Solventum 2025. Solventum, the S logo are trademarks of Solventum or its affiliates. 3M, the 3M logo, Attest and Comply are trademarks of 3M. Other trademarks are the property of their respective owners. 70-2011-7096-9 Rev 5/25
*Refer to 3M™ Attest Auto-reader 490 Operator Manuals and 3M™ Attest Biological Indicator 1295, 1491 and 1492V Instructions for Use for complete instructions and other important information. Refer to the indications for use provided in the biological indicator Instructions for Use. The indications for use should align with the sterilization cycle to be monitored. This Quick Reference Guide applies to 3M™ Attest” Auto-reader 490 having software version 4.2.7 or greater. Refer to the Instructions for Use for complete instructions and other important information related to the use of all other products included in this Guide.
Health Care Help Line 1-800-228-3957
Learn more at go.Solventum.com/sterilization
Solventum Medical Surgical
3M Center, Building 275
2510 Conway Avenue East
St. Paul, MN 55144 USA
Documents / Resources
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solventum 490 System Attest Auto Reader [pdf] User Guide 490, 490 System Attest Auto Reader, 490, System Attest Auto Reader, Attest Auto Reader, Auto Reader, Reader |