SARTORIUS Sartobind IEX Lab Fast Flexible and Future Proof Charge Based
Power Up: Fast, Flexible and Future-Proof Charge-Based Separations
Product Information
Sartobind® IEX Lab incorporates state-of-the-art in ion exchange chromatography (IEX) membranes, enabling flexible biomolecule purification that is both fast and scalable. It is ideal for protein, virus and nucleic acid research, as well as the development of downstream processes to purify novel therapies and vaccines before scaling up to clinical and commercial production.
For research and molecule development, IEX on resin columns requires additional preparation steps, and the availability and maintenance of specialist equipment. High operating costs, low flow rates and yields, especially for larger molecules, together with a lack of scalability limits overall efficiency.
Sartobind® IEX Lab is ready-for-use in research and development laboratories. Biomolecules are transported to the high-capacity ligands mainly by convective flow, enabling high flow rates.
This results in time savings up to 89% per cycle, while effective regeneration supports reproducible purifications over 100 cycles. Compared to columns, it is possible to purify up to 23X more protein over the lifetime of each Sartobind® IEX Lab unit, resulting in 78% savings on cost of goods.
Adapter-free inline prefiltration and reliable, syringe-driven purifications reduce process time even further, while the same Sartobind® IEX technology is available in capsule and cassette formats for convenient scale up to GMP production.
Features
The Fastest Matrix
Full speed ahead. Rapid flow rates and reduced residence times take you from equilibration to pure molecule in a matter of seconds, not minutes.
Productivity Boost
Push the limits. Macroporous membranes with high ligand densities deliver maximum yields of even the largest viruses without size exclusion effects.
Plug and Play
Collapse your workflow. Ready-for-use units avoid the need for column packing and buffer degassing, while enabling convenient inline prefiltration.
Process-Ready Technology
Seamless scalability. Future-proof by design, ensuring ease-of-transfer for your most promising targets to clinical and commercial production.
Flexibility Built In
Accept no limitations. Enhanced pressure resistance in a platform that ensures reliable purification with or without a liquid chromatography system.
Applications
Capture Purification
- Extracellular vesicles and particles
- Plasmids and oligonucleotides
- Proteins and peptides
- Viruses and viral vectors
Flowthrough Polishing
- Aggregate, DNA and HCP removal
- Buffer and sample depyrogenation
- Leached protein A removal
- Virus clearance
Operating Principle
Ion exchange chromatography (IEX) can be used to separate a molecule of interest (MOI) from impurities based on differences in charge. Because the net charge of most molecules can be adjusted simply by changing the pH of the mobile phase, IEX can be used to purify virtually any biomolecule. If the mobile phase creates conditions that favor a net charge on a molecule opposite to that of the IEX ligand, that molecule will be captured. This can be either the MOI or contaminants, depending on the preferred mode of operation (Figure 1).
Figure 1: A. In capture purification, the MOI, red, binds to ligands of opposite charge, blue, while impurities of similar charge, light blue, are removed. A subsequent wash step can increase the purity of the MOI before elution at high ionic strength. B. In contrast, flowthrough polishing is a single-step method in which impurities, red, bind to the ligands, blue, and the MOI, light blue, passes through the membrane.
Technical Specifications
Materials
- Adapters Polyether ether ketone (PEEK)
- Caps Polycarbonate (PC)
- Fittings³ Polyamide (PA)
- Housing Polypropylene (PP)
- Ligand Diethylamine (D), quaternary ammonium (Q), sulfonic acid (S)
- Membrane Regenerated cellulose (RC)
- Membrane support Polyethylene terephthalate (PET)
- Tubing³ Polyvinyl chloride (PVC)
- Packaging Cardboard (PAP)
Equipment and Consumable Requirements
| Benchtop Purification (Syringe or Pump) | Rapid Cycling (System) | |
| Operation | Syringe⁴ Pump drive, pump head, tubing | LC system, fraction collector |
| Prefiltration⁵ | Sartolab® RF or Minisart® Sartolab® RF or Minisart® | Sartolab® RF or Minisart® |
| Fraction collection | Tubes Tubes | Tubes or plates |
- One pair of adapters is included in each pack of Sartobind® IEX Lab, for connection to LC systems that utilize male UNF 10-32 fittings.
- 29 mg/mL for Sartobind® D | Q, and 25 mg/mL for Sartobind® S. Measured at 10% breakthrough for BSA in 20 mM Tris-HCl pH 6 (D) or 7.5 (Q), and for lysozyme in 10 mM potassium phosphate pH 7 (S).
- Components of pump tubing (order number VF-ATD0001-1).
- With capacity ≥10 mL and male Luer lock connector.
- For inline prefiltration, use a Minisart® syringe filter (order number S6534-FMOSK).
Operating Conditions
Ordering Information
Description Package Contents Ligand and Order No.
| Sartobind® IEX Lab, 0.4 mL | 4 units
1 pair UNF adapters 1 quick start guide |
Q S | 93IEXQ42GB-12—A
93IEXS42GB-12–A |
| Sartobind® IEX Lab, 2.1 mL | 2 units | D | 93IEXD42DB-12—V |
| 1 pair UNF adapters | Q | 93IEXQ42DB-12—V | |
| 1 quick start guide | S | 93IEXS42DB-12–V | |
| Sartobind® IEX Lab, 2.8 mL | 1 unit | Q | 93IEXQ42BC-12 |
| 1 pair UNF adapters | S | 93IEXS42BC-12 | |
| 1 quick start guide | |||
| Sartobind® Lab LC system adapter kit | 1 pair UNF adapters | SBLAAU01-1 | |
| Peristaltic pump | 1 unit | VF-APD0001-1 | |
| 1 power cable with region-specific plug | |||
| Peristaltic pump head for 1.6 mm tubing | 1 unit | VF-APH0001-1 | |
| 1 quick start guide | |||
| Pump tubing | 1 unit | VF-ATD0001-1 |
- Equivalent to 2 – 12, 10 – 63 or 14 – 84 mL/min for units with 0.4, 2.1 or 2.8 mL MV, respectively.
- When using inline prefiltration, adjust the operating pressure based on the lowest specification given for Minisart® or Sartobind® Lab.
- When loading directly from bioreactors, short-term operation up to 37 °C is possible.
- Long- | short-term pH compatibility is 4-13 | 2-14 for Sartobind® D, 2-12 | 2-14 for Sartobind® Q, and 4-13 | 3-14 for Sartobind® S. Avoid oxidizing agents.
- Based on recommended buffer volumes and flow rates for units with 0.4, 2.1, and 2.8 mL MV, with 10 mL load.
Germany
Sartorius Lab Instruments GmbH & Co. KG
Otto-Brenner-Strasse 20
37079 Goettingen
Phone +49 551 308 0
USA
Sartorius Corporation
565 Johnson Avenue
Bohemia, NY 11716
Phone +1 631 254 4249
Toll-free +1 800 635 2906
For further contacts,
visitsartorius.com
Specifications subject to change without notice. Copyright Sartorius Lab Instruments GmbH & Co. KG. Status: 08 | 2024
Documents / Resources
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SARTORIUS Sartobind IEX Lab Fast Flexible and Future Proof Charge Based [pdf] Owner's Manual Sartobind IEX Lab, IEX Lab, Sartobind IEX Lab Fast Flexible and Future Proof Charge Based, Fast Flexible and Future Proof Charge Based, Future Proof Charge Based, Charge Based |
