Peptide Test Reconstitution and Filtering Starter Kit
Product Specifications
- Product Name: Reconstitution and Filtering Starter Kit
- Usage: Reconstituting and filtering water-soluble peptides
- Application: Laboratory research
- Components: Reconstitution tools, filtering equipment
Product Usage Instructions
Procedure for Reconstituting and Filtering Peptides
This procedure outlines the steps to reconstitute and filter water-soluble peptides using the Reconstitution and Filtering Starter Kit. It can be used on its own, but is best followed alongside the instructional video: Watch
Step 1: Prepare Your Workspace
- Disinfect all surfaces and packaging of all materials in the kit that you will use for this cycle with Curad Alcohol Prep Pads.
- Wear a new pair of exam gloves (not supplied) and sanitise hands with Curad Alcohol Prep Pads.
Step 2: Reconstitute the Peptide
- Remove the caps from the lyophilised peptide vial (not supplied) and bacteriostatic water (30ml vial).
- Wipe the vial stoppers from the step above, plus a 2/3 ml sterile vial with an alcohol prep pad, and let them dry.
- Attach a 25g 5/8″ Dynarex needle to an Exel 3ml Luer Lock Syringe.
- Draw the desired amount of bacteriostatic water (30ml vial) into the syringe.
- Slowly inject the water into the lyophilised peptide vial along the glass wall. (Note: The peptide vial may be under vacuum and “suck in” the bacteriostatic water—this is normal.)
- Swirl gently—do not shake—to dissolve the peptide. Let it sit for 10-15 minutes minimum until the solution is clear and bubbles have subsided.
- Remove the needle.
Step 3: Draw the Reconstituted Solution
- Attach a 25g 1-1/2″ Dynarex needle to your Exel 3ml syringe.
- Withdraw the reconstituted peptide solution from the vial, ensuring you reach the bottom corner of the vial with the needle.
- Remove the needle.
Step 4: Prepare for Filtration
- FIRMLY attach a Tisch Scientific .22μm PES 4mm syringe filter to the syringe.
- Attach a fresh 25g 5/8″ needle to the filter’s outlet.
- Insert a vent needle (25g 5/8″) into the receiving sterile vial to equalise pressure.
- Attach a Tisch Scientific .22μm PES 4mm syringe filter to your vent needle.
Step 5: Filter the Solution
- Slowly press the plunger to filter the solution into the sterile vial.
- If filtering a larger volume (>3ml) or a thicker peptide solution, switch to a Tisch Scientific .22μm PES 13mm filter and use the 4mm filter for the vent.
Step 6: Flush the Filter
- Leave the needle and filter in the vial and unscrew and remove the syringe.
- Use a new 25g 5/8″ needle to draw up a small amount of bacteriostatic water into the syringe.
- Remove the needle.
- Holding the syringe upright, draw in air until the plunger hits the built-in stop at 3ml.
- Firmly reattach the syringe to the filter and forcefully push the bacteriostatic water/air through the filter.
- Label the vial and store as needed.
Final Step
- Dispose of all used needles in a sharps container. Your peptide is now reconstituted and filtered.
Frequently Asked Questions
Q: Can the filtering equipment be reused?
A: The filtering equipment provided in the starter kit is designed for single-use to maintain optimal filtration efficiency.It is recommended to use a new filter for each reconstitution process.
Q: Is it necessary to watch the instructional video?
A: While it is not mandatory, following the instructional video alongside the written procedure can help ensure accurate reconstitution and filtering of peptides.
Documents / Resources
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Peptide Test Reconstitution and Filtering Starter Kit [pdf] Instructions Reconstitution and Filtering Starter Kit, and Filtering Starter Kit, Filtering Starter Kit, Starter Kit |