1. Inleiding
The AmScope D120A-MS is a dual-view compound monocular microscope designed for high magnification observation in biological and educational applications. It features a versatile viewing head, multiple objective lenses, Brightfield illumination, and a mechanical stage for precise specimen manipulation. This manual provides essential information for the proper setup, operation, and maintenance of your microscope.
2. Veiligheidsrichtlijnen
- Ga altijd voorzichtig om met de microscoop. Vermijd abrupte bewegingen of stoten.
- Ensure the microscope is placed on a stable, level surface to prevent tipping.
- Connect the power cord to a grounded electrical outlet of the correct voltage (110 V).
- Do not touch optical surfaces with bare hands. Use lens paper or a soft, lint-free cloth for cleaning.
- Unplug the microscope from the power source before cleaning or performing any maintenance.
- Keep the microscope away from direct sunlight, high temperatures, dust, and corrosive chemicals.
- Supervise children or inexperienced users to ensure safe operation.
3. Inhoud van het pakket
Pak alle onderdelen voorzichtig uit en controleer of de volgende onderdelen aanwezig zijn:
- AmScope D120A-MS microscope with mechanical stage
- WF10x eyepiece
- WF16x eyepiece
- 4x DIN achromatic objective
- 10x DIN achromatic objective
- 40xS DIN achromatic objective (spring-loaded)
- 100xS (oil) DIN achromatic objective (spring-loaded)
- (2) Color filters
- Reservelamp
- Stofkap
- Gebruiksaanwijzing (dit document)
4. Componentidentificatie
Familiarize yourself with the main parts of your AmScope D120A-MS microscope:
Figuur 4.1: Overview of the AmScope D120A-MS microscope. This image displays the complete microscope assembly, including the base, stage, objective lenses, and dual-view monocular head.
- Eyepiece (Ocular): Waar je naar kijkt view the specimen. The D120A-MS includes WF10x and WF16x eyepieces.
- Monoculair Viewhoofd: The upper part of the microscope containing the eyepiece tube. This model features a dual-view head with a fixed 45-degree inclined tube and a vertical port.
- Revolving Nosepiece: Holds the objective lenses and allows for easy switching between magnifications.
- Objectieve lenzen: The primary magnification components (4x, 10x, 40xS, 100xS oil).
- Mechanische Stage: A platform where the specimen slide is placed, with controls for precise X-Y movement.
- Stage Clips: Secure the specimen slide on the mechanical stage.
- Coarse Focus Knob: Large knob for rapid focusing adjustments.
- Fine Focus Knob: Small knob for precise focusing adjustments.
- Illuminator (Light Source): Located beneath the stage, provides light for viewing. This model uses variable-intensity tungsten illumination.
- Abbe-condensor: Located below the stage, focuses light onto the specimen. Features a 1.25 NA and an iris diaphragm.
- Irisdiafragma: Controls the amount of light passing through the specimen.
- Baseren: The stable bottom support of the microscope.
5. Montage en opstelling
Follow these steps to set up your microscope for initial use:
- Uitpakken: Carefully remove the microscope and all accessories from the packaging. Retain the original packaging for future transport or storage.
- Plaatsing: Place the microscope on a sturdy, level surface, away from vibrations and direct sunlight.
- Installatie van het oculair: Insert the desired eyepiece (WF10x or WF16x) into the inclined eyepiece tube of the monocular head. Gently twist to secure it.
- Objectieve installatie: The objective lenses are typically pre-installed. If not, carefully screw each objective into the revolving nosepiece in increasing order of magnification (4x, 10x, 40xS, 100xS). Ensure they are finger-tight.
- Stroomaansluiting: Connect the power cord to the microscope's power input and then to a standard 110V electrical outlet.
- Stofkap: When not in use, cover the microscope with the provided dust cover to protect it from dust and debris.
Figuur 5.1: Van dichtbij view of the objective lenses mounted on the revolving nosepiece. This image shows the 4x, 10x, 40xS, and 100xS (oil) objectives in position.
6. Basisbediening
This section outlines the fundamental steps for observing specimens with your microscope.
- Inschakelen: Flip the power switch located on the base of the microscope. The illuminator should light up.
- Verlichting aanpassen: Use the variable intensity control knob to set the desired brightness. Adjust the iris diaphragm on the Abbe condenser to optimize contrast and resolution for your specimen.
- Place Specimen: Rotate the revolving nosepiece to select the lowest power objective (4x). Lower the mechanical stage using the coarse focus knob. Place a prepared specimen slide onto the mechanical stage en bevestig het met de stagen clips.
- Position Specimen: Gebruik de XY'stage control knobs to move the slide until the area of interest is centered under the objective lens.
- Focussen:
- While looking through the eyepiece, slowly raise the stage met behulp van de coarse focus knob until the specimen comes into rough focus.
- Gebruik de fine focus knob to achieve a sharp, clear image.
- Vergroting wijzigen: Once focused at low power, rotate the revolving nosepiece to switch to a higher power objective (e.g., 10x, 40xS). Due to the parfocal design, only minor adjustments with the fine focus knob should be needed to bring the image back into sharp focus.
- Using the 100xS Oil Immersion Objective:
- After focusing with the 40xS objective, rotate the nosepiece halfway between the 40xS and 100xS objectives.
- Place a small drop of immersion oil directly onto the center of the specimen slide where the light passes through.
- Carefully rotate the 100xS objective into the oil drop until it clicks into place. The objective should be immersed in the oil.
- Gebruik alleen de fine focus knob to bring the image into sharp focus. Do not use the coarse focus knob with the 100xS objective.
- After use, clean the 100xS objective and the slide immediately with lens paper moistened with lens cleaning solution to remove all immersion oil.
- DubbelView Functionaliteit: The vertical port on the monocular head can accept a second eyepiece for simultaneous viewing by another person or a camera mount for digital image capture (eyepiece and camera sold separately). Dioptric adjustment on the upright tube accommodates individual eye-strength differences.
Figuur 6.1: The AmScope D120A-MS microscope demonstrating its digital image capture capability by connecting to a laptop. This setup utilizes the vertical port for a camera attachment.
7. Onderhoud en verzorging
Proper maintenance ensures the longevity and optimal performance of your microscope.
- Lenzen reinigen: Use only specialized lens paper and lens cleaning solution for optical surfaces. Gently wipe in a circular motion. Never use abrasive cloths or harsh chemicals.
- Algemene schoonmaak: Veeg de behuizing van de microscoop af met een zachte, droge doek.amp Doek het doekje om te voorkomen dat er vocht in de elektrische componenten komt.
- Opslag: Always cover the microscope with the dust cover when not in use. Store it in a dry, dust-free environment.
- Lamp vervangen: If the illuminator bulb burns out, unplug the microscope. Refer to the base of the microscope for access to the bulb compartment. Carefully replace the old bulb with the provided spare bulb, ensuring not to touch the glass part of the new bulb with bare hands.
- Mechanische onderdelen: Do not attempt to lubricate or disassemble any mechanical parts. If issues arise, contact customer support.
8. Probleemoplossing
In dit gedeelte worden veelvoorkomende problemen besproken waarmee u te maken kunt krijgen.
| Probleem | Mogelijke oorzaak | Oplossing |
|---|---|---|
| Geen verlichting | Power cord disconnected, power switch off, bulb burnt out | Check power connection, turn on switch, replace bulb |
| Afbeelding is wazig of onscherp | Incorrect focus adjustment, objective not fully engaged, dirty lens | Adjust coarse/fine focus, rotate nosepiece until objective clicks, clean lenses |
| Donker veld van view | Iris diaphragm closed, illuminator too dim, condenser too low | Open iris diaphragm, increase illuminator brightness, adjust condenser height |
| Stof of vlekken in view | Dust on eyepiece, objective, or slide | Clean eyepiece, objective, or slide with lens paper |
| Cannot focus at 100x | No immersion oil, air bubbles in oil, coarse focus used | Apply immersion oil, remove air bubbles, use only fine focus |
9. Technische specificaties
Detailed specifications for the AmScope D120A-MS microscope:
- Hoofd: Compound dual-view monocular, 45-degree inclined, 360-degree rotatable
- Oculairen: WF10x, WF16x (with pointer)
- Doelstellingen: 4X, 10x, 40xS (spring), 100xS (oil, spring) DIN achromatic
- Vergrotingsbereik: 40X - 1600X
- Stage: Mechanische stage with X-Y axis movement, stage stop
- Verlichtingsconfiguratie: Transmitted (lower)
- Condensator: Abbe, 1.25 NA
- Diafragma: Iris diafragma
- Lichtbron: Tungsten (incandescent), variable intensity
- Verlichtingstype: Helder veld
- Stroom: 110V
- Materiaal: Metal, tungsten
- Afmetingen (H x B x D): 15 x 7.5 x 5.125 inch (ongeveer)
- Gewicht: 10 pond
10. Garantie en klantenondersteuning
For warranty information, technical assistance, or to purchase replacement parts, please contact AmScope customer support. Refer to the AmScope website or your purchase documentation for current contact details.
