CR-22P Microplate Centrifuge

1. Introduction

The CR-22P is a microprocessor-controlled digital 96/384 well micro-plate centrifuge designed for sample separation and preparation in various applications such as molecular biology, genetic engineering, microbiology, genomics, immunology, and in-vitro diagnostics. It features a maintenance-free Brushless DC motor and an imbalance detection safety feature. The centrifuge is compatible with different types of standard-sized microplates.

2. Intended Use

This benchtop, two-place microplate centrifuge is intended for use with PCR plates, ELISA plates, cytokine assay plates, sample quantifications, and short spin-downs in molecular biology, genomics, proteomics, diagnostic labs, forensic laboratories, and hospitals for routine plate centrifuging applications.

Note: Please read this user manual carefully before using the instrument. This manual assists with operation and care, not repair. For repairs, contact the supplier.

3. Features

• Maintenance-free Brushless DC motor for long life.
• Microprocessor controlled with digital variable speed and time setting, and last run memory function.
• Suitable for different work protocols with appropriate rotor choices.
• Precise RPM setting from 500 to 2500 RPM with a least count of 100 RPM.
• Automatic imbalance detection for safe operation.
• Lid lock safety: Lid releases automatically upon run completion.
• Compact design with low loading height for ease of use.
• P1 & P2 defined program features.

4. Standard Accessories

• Power Adaptor: Input voltage - 100-240V, 50/60Hz; Output voltage: 24V ⟷ 1.5A
• T-Allen Key
• Rotor
• Product user manual & warranty card

5. Technical Specification

6. Safety Precautions

• Never use the centrifuge in any manner not specified in this manual.
• Using the equipment in a manner not specified by the manufacturer may void the warranty.
• Never move the centrifuge during operation.
• Ensure the rotor and rotor lid are always securely fastened. If unusual noise occurs, stop the centrifuge immediately, check and fasten the rotor fitment.
• Rotors must be loaded symmetrically. Each plate should be counter-balanced by another plate of the same weight.
• Do not use a centrifuge or rotor that has not been correctly installed or is damaged.
• Repairs must only be performed by authorized service technicians.
• Use only recommended original rotors and spare parts.
• The centrifuge must be used only for its specified application. Do not operate in hazardous or flammable environments, or use to centrifuge explosive or highly reactive media. Keep potential hazardous materials away from the clearance area.
• If liquids spill on the rotor or in the rotor chamber, clean the centrifuge carefully before reuse.
• Visually inspect plates for material damage before centrifugation. Damaged plates can cause sample loss and imbalance, potentially damaging the centrifuge.
• Do not exceed the centrifuge's specified capacity.
• Do not lean on the equipment.
• When moving the centrifuge from a cold room to a normal room, run it for 30 minutes in the cold room beforehand to avoid condensation. Alternatively, allow it to warm up in the lab for at least three hours without plugging it in.
• Ensure the plate lid is closed tightly before centrifugation. Open lids can be torn off during operation.
• Rotors and adapters are subject to extreme mechanical strain. Check rotors for any signs of damage, corrosion, or mechanical damage before use.
• Do not fill plates while they are in the rotor; liquid spillage may damage the device.
• Ensure the power supply is properly earthed.
• In case of contamination with aggressive agents, clean the rotor immediately with a natural cleaning liquid. Contact a service technician if any damage is observed.
• Before using cleaning or decontamination methods not mentioned by the manufacturer, contact them to ensure they will not damage the centrifuge.
• Use only the provided power adaptor for this centrifuge.

Note: Insert both microplates for balancing the rotor and reducing vibrations.

7. Installation

The centrifuge is shipped in a packaging box. Remove the upper packaging and lift the centrifuge from the bottom. Ensure all packaging accessories are removed from the rotor chamber before first use. The rotor is pre-installed; confirm it is firmly tightened. Keep all packaging for at least 2 years for warranty purposes.

Note: Instructions for transportation after delivery are to be handled by the customer.

Installation Requirements:

• Place the centrifuge on a flat, non-slippery, and leveled surface.
• Ensure all four feet stand firmly on the surface.
• Ideal ambient temperature is 20°C ± 5°C; avoid direct sunlight.
• Maintain a clearance of at least 10 cm on the sides and 30 cm behind the unit for cooling efficiency.
• Keep away from heat or water sources.
• Ensure the operating location allows for easy access and operation.

Connecting Power Adaptor:

1. Connect one end of the power adaptor to the rear of the centrifuge and the other to the power supply.
2. Rotate the adaptor nut clockwise to tighten it to the centrifuge. Ensure the power switch is OFF.
3. Match the rib on the plug with the slot on the centrifuge's adaptor port before inserting.
4. Once matched, gently push the plug in. Do not force it.
5. Turn the upper ring clockwise to secure the fitment.

8. Standard Parts Listing

Front View:

• 1. Lid Release Button
• 2. Display Screen
• 3. Keypad
• 4. Power Switch
• 5. Adaptor Port

Rear View:

• (Refer to image showing adaptor port and power switch)

Bottom View:

• Rubber Mounts / Base Mounts

9. User Interface & Display

10. Rotor Installation

Rotor Removing and Replacing Process:

The rotor comes pre-installed. To remove or replace it:

Removing Rotor:

1. Do not attempt to remove or loosen the rotor with bare hands.
2. Use the T-Allen Key to loosen the rotor nut by turning it anti-clockwise.

Replacing Rotor:

1. Load the rotor vertically onto the motor shaft.
2. Place the rotor nut in the center hole of the rotor onto the motor shaft.
3. Insert the T-Allen Key into the rotor nut and turn clockwise to tighten or anti-clockwise to loosen.

Note: Ensure the rotor is firmly tightened before running.

11. Balancing the Rotor

1. Always balance the rotor before operation. Load centrifuge plates symmetrically.
2. Ensure samples in the plates have equal volumes.
3. Symmetrical loading is crucial to prevent vibration or imbalance, which can damage the centrifuge and harm the user.
4. If plates are not loaded symmetrically, the imbalance detector will stop the centrifuge (Err 55). Load plates symmetrically and restart.
5. Incorrect plate loading can lead to accidents.

Note: 'Plates Holder' are part of the Rotor.

12. Operating the Centrifuge

Switching On:

After connecting the power adaptor, switch ON the main power supply and the switch at the rear of the centrifuge. Ensure the rotor is securely fitted before use. The centrifuge will not operate with the lid open.

Note: Maintain a 3-second gap between switching OFF and ON again. Do not switch OFF and ON instantly.

Speed Setting:

After closing the lid, press the 'RPM/RCF' button to select speed setting (RPM or RCF). Use the '+' and '-' buttons to adjust the value. The speed range is 500 to 2500 RPM. The set value is stored automatically after approximately 1 second of inactivity. Speed can be changed during operation by pressing 'RPM/RCF' and using the +/- buttons. Long press 'RPM/RCF' to toggle to RCF setting.

Time Setting:

Operating time can be set from 1 minute to 99 minutes or in continuous mode (' ][: ][ '). Press the 'TIME' button and use the '+' and '-' buttons to adjust the value. The timer is a countdown timer displaying 'Min/Sec'.

Start and Stop Operation:

Press the 'START' button to begin operation. Press the 'STOP/OPEN' button to stop the ongoing operation. The lid opens automatically after the operation stops.

Program Mode & Line Setting:

Press 'P1' or 'P2' once to enter program mode. Use 'RPM/RCF' and '+/-' to adjust speed/RCF, and 'TIME' to adjust time. Set values are saved after 5 blinks. Press 'START' to run the program. Long pressing 'P1' or 'P2' returns the centrifuge to normal mode.

Changing Settings During a Run:

Settings can be changed during operation. Pressing a function button (TIME, RPM, RCF) allows modification of the current value. New values are activated after entry and are stored upon confirmation (5 blinks).

Opening Centrifuge Lid in Power Failure:

Disconnect the centrifuge from the main supply. Wait for the rotor to stop completely. Pull out the emergency lid release pin to open the lid. After opening, push the thread back into the hole and refit the emergency release cap.

13. Troubleshooting

The centrifuge has a self-diagnostic function. Error/warning codes displayed on the screen indicate malfunctions:

Notes:

• If the centrifuge runs slow or gets hot due to overcurrent, restart and check.
• Do not use liquids with a density higher than 1.2 g/ml for full load operation.

14. Warranty Statement

This product is warranted against defects in material and workmanship for two (2) years from the date of purchase. The warranty covers repair upon prompt notification under the following conditions:

• The product is used for its intended purpose and within the guidelines of this manual.
• The warranty does not cover damage from accident, neglect, misuse, improper service, natural forces, or causes not arising from defects in material or workmanship.
• Incidental or consequential damages, commercial loss, or other damages from product use are not covered.
• Any non-factory modification voids the warranty.
• The buyer and customer are responsible for product use and supervision. Returned products must be well-packed, insured, and shipped at the buyer's expense.
• Some states do not allow limitations on implied warranties or incidental/consequential damages. This warranty grants specific legal rights.
• This warranty is expressly in lieu of all other warranties, expressed or implied. There is no warranty of merchantability or fitness for a particular purpose.
• The warranty is valid only for the original purchaser.
• Products returned without proper authorization will not be accepted. Return items postage prepaid in original or suitable packaging, padded to avoid damage.
• The company is not responsible for damage incurred by improper packaging.
• Warranty registration with the supplier within 30 days of purchase is required.

For your reference:

Serial No.: _______________

Purchase Date: _______________

Supplier: _______________

15. Product Disposal

Observe relevant legal regulations when disposing of the product. Electrical and electronic devices are regulated by national regulations based on EU Directive 2012/19/EU (WEEE). Devices supplied after 13.06.05 in the business-to-business sphere, to which this product is assigned, may not be disposed of in municipal or domestic waste. They are marked with the following symbol: ♺.

Disposal regulations vary within the EU; contact your supplier if necessary.