User Guide for GALENVS models including: WW0016-12, Wastewater DNA and RNA Extraction Kit, WW0016-12 Wastewater DNA and RNA Extraction Kit, DNA and RNA Extraction Kit, RNA Extraction Kit, Extraction Kit

qsgWastewater0016 v2 20221007

[Semi-Automated 16 samples] Quick Start Guide: Wastewater DNA/RNA Extraction Kit (WW0016)

Wastewater DNA/RNA Extraction Kit | Galenvs | Fullstack Biotech

[Semi-Automatique 16 échantillons] Guide de démarrage rapide: Kit d’extraction d’ADN/ARN à partir d’eaux usées (WW0016)

Guides & Protocols | Galenvs | Fullstack Biotech

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Wastewater DNA/RNA Extraction Kit
Quick Start Guide

Binding Buffer (600µl) Columns 1 & 7
Binding magnetic nanoparticles (30µl) Columns 2 & 8

Wash #1 Buffer (600µl) Columns 3 & 9
Wash #2 Buffer (600µl) Columns 4 & 10
Elution Buffer (90µl) Columns 6 & 12

Add Lysis supernatant

Elution Add Lysis supernatant

Elution

WWKit miQron protocol parameters

Step Name Column Volume Time Mixing Speed Dry Time Magnet Capture Temp

(µl) (sec) (1­10)

(sec) Time (sec)

-Load-

Bead Pickup 2 & 8 30 60 Medium

0

1 x 30 sec OFF

Binding

1 & 7 1000 300 Medium

0

5 x 30 sec OFF

Wash #1

3 & 9 600 60 Medium

0

2 x 30 sec OFF

Wash #2

4 & 10 600 60 Medium

120

2 x 30 sec OFF

Elution

6 & 12 100 300

Low

0

2 x 30 sec 65ºC

-Unload-

2 & 8

1

Transfer 10ml of wastewater sample to 15ml conical tube. Add 100µl of Concentration

Buffer to sample. Invert 5 times.

Add 75µl of Concentration Beads. Invert 5 times and incubate for 10 mins. Invert 3 more times at the 5 min mark.

Concentration Buffer Concentration Beads

2

Place sample on 15ml magnetic rack to capture beads, then discard supernatant.

Remove tube from magnetic rack.

3

Resuspend beads in 1ml RNase-free water, then transfer the mixture to a clean 2ml

centrifuge tube.

Place tube on 2ml magnetic rack to capture beads, then discard supernatant.

Wash Buffer #1

4

Remove tube from magnetic rack and resuspend beads in 400µl of Lysis Buffer.

Vortex at max speed for 5 mins, then separate beads using a 2ml magnetic rack.

5

Avoiding pellet, transfer up to 400l of supernatant to the Binding Buffer wells

(Columns 1 & 7).

You can add up to 16 samples.

6 Place plate into the miQron, taking care
that the label is facing outward.

Lysis Buffer

7 Insert two combs.

8 Select the WWKit protocol and press

Run Run Report
Settings

05-19-2021 11:16 WWKit

When program is complete, remove plate from miQron and discard combs.

Columns 6 & 12 contains the purified DNA/RNA elution.

Wastewater DNA/RNA Extraction Kit

1.0