ALERT One

Quick Start Guide

1. Prepare vial and reagent

   Prepare sample and reagent (p.2). Mix thoroughly.

2. Insert vial in the device

   Remove analyzer cap. Insert vial fully. Replace analyzer cap.

3. Power the analyzer

   Requirements: USB-C 5V DC, 15W. Use supplied adapter or battery pack. Battery must have trickle charge capability activated.

4. Start the measurement

   Navigate to Measure menu. Follow instructions below.

Menu Navigation

* While performing a measurement, or in the History menu

Starting a Measurement

Step 1: Add sample to vial. Mix thoroughly. Insert and cover. Continue.

Step 2: Set Water temp? [Yes/No]

If 'No' is selected: Adjust water temp only if sample not at room temperature.

Step 3: Wait (2-14h) for measurement to end. Do not tilt!

Step 4: View results: E. coli concentration per 100mL and corresponding risk level*.

Risk levels: LOW, MEDIUM, HIGH, VERY HIGH.

*Note: Risk thresholds are custom defined in the Settings Menu.

Exploring Data History

Navigate between measurements using [Up Arrow] and [Down Arrow].

Settings Menu

• Clear Memory: Delete all measurements from device memory.
• Set Threshold*: Adjust the risk thresholds.
• Date and Time: Change device date and time.
• Device Info: Display serial number and firmware versions.
• Download data: Send datasets to serial port (computer app required).
• Sound: Toggle sound alerts On or Off.
• Display T.C.: Toggle display of Coliform results On or Off.
• Calib*: Select a specific calibration (see below).
• Volume (ml)*: Set the final volume (freshwater: 50ml, seawater: 37ml).

* Will affect future measurements.

Advanced Data Display: Long Press (5s)

View Fluorescence curve (E. coli signal), Absorbance curve (Coliform signal), and Raw signals and algorithm version.

Available calibrations for use with ALERT One*

* Not all may be present. New calibrations under constant development. Inquire with Fluidion for available upgrades.

• 1.6.40 E.coli TC River - Plastic v2 (EV2024): For freshwater (river, lake).
• 1.6.41 E.coli TC Seawater- Plastic v2 (EV2024): For seawater (ocean, brackish).

Sample and Reagent Preparation*

*Shown for single-use plastic vial. Glass vials currently not supported.

Freshwater samples

Add bioreagent. Add 50mL of freshwater sample (to molded line).

Seawater samples

Add bioreagent. Add 27mL of sterile water (to first line). Add 9mL of seawater sample (to second line).

Sample and vial disposal procedure

Dispose used vial as biological waste according to local regulations. If available, use appropriate biological waste collection services. Only when no biological waste collection service available, follow the vial disinfection procedure below:

Add 1ml of concentrated liquid bleach to vial containing sample. Close, shake, wait 15 minutes. Pour content in sanitary drain. Dispose of single-use plastic vials as trash, or, if available, recycle as polypropylene (PP) ♻️.

Important Safety Information:

• Read the reagent Material's Safety Data Sheet (MSDS) before use.
• Wear protective gloves when dealing with potentially contaminated samples.
• Protect clothing from bleach projections to avoid staining.
• Keep instrument and battery dry and protected from the elements.
• Ensure that reagent is within its validity period.
• Do not leave device in direct sunlight. Max. ambient temperature: 37°C.
• Do not tilt the device while performing a measurement. Alarm will sound. ⚠️

Danger
Biohazard ☣️

Product Warning

The reagents used with this product cause rapid multiplication of E.coli and other potentially harmful biological substances. Used samples and reagents should never be disposed directly into the environment or drinking water sources. This product and all associated reagents and consumables should never be used or accessed by unsupervised children. ??‍?‍?‍?