Notices
Document Identification
Doc No D0026036
DE50653432
October 2022 Revision A.00
Copyright
Agilent Technologies, Inc. 2022
No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.
Agilent Technologies, Inc.
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Santa Clara, CA 95051
Software Revision
This guide is valid for MassHunter Acquisition for LC/TQ 12.0, MassHunter Acquisition for LC/TOF and LC/Q-TOF 11.0, MassHunter Qualitative Analysis 10.0, and MassHunter Quantitative Analysis 12.0.
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Safety Notices
CAUTION
A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.
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A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met.
In This Book
This document details the minimum computer hardware, software, and network requirements as well as the minimum instrument firmware revisions required to run an Agilent MassHunter Workstation or Agilent MassHunter Networked Workstation. Also included are the supported instrument types and Windows operating system configurations.
Table 1 Terms and abbreviations used in this document
| Term | Description |
|---|---|
| Content Management | Database to manage your analytical data. The database is provided as a component of OpenLab Server. Always used in Client/Server systems, optional for Workstations. |
| Control Panel | Control Panel for OpenLab Shared Services |
| Microsoft Control Panel | Part of the Microsoft Windows operating system |
1 Hardware and Software Requirements
This chapter contains the hardware and software requirements for the different components of a MassHunter system.
Depending on the type of installation, you may need different hardware components. The following descriptions show the required components for each scenario.
Description of Figure 1. MassHunter Workstation: A diagram showing a MassHunter Workstation setup. It depicts a computer connected to a network (Customer LAN) and to analytical instruments (LC/MS system) via a switch. All required components are installed on the workstation.
Description of Figure 2. Networked Workstation system: A diagram illustrating a networked workstation system. It shows multiple networked workstations, each connected to analytical instruments, and all workstations connected to a central OpenLab Server or OpenLab ECM XT server via a network. The system includes both Networked Workstations and an OpenLab Server or OpenLab ECM XT server or ECM 3.x server.
Software
General Software Requirements
| Component | Details |
|---|---|
| .NET framework |
|
| Web browser |
|
| Anti-virus software* |
|
* The listed anti-virus software has been tested to be compatible with the MassHunter software described in this document. While other third-party AV solutions may also be compatible, they have not been tested, and compatibility cannot be guaranteed.
Windows Compatibility
Only these Windows operating systems are supported:
- Windows 10 Professional Semi-Annual Channel: 1909 or newer.
- Windows 10 Enterprise LTSC editions: 1809 or newer.
- Windows 11 Pro (or Pro for Workstations) General Availability Channel: 21H2 or newer.
Computer Hardware
Disk Space
Disk space requirements depend based on the number and type of instruments, archival frequency, and the method settings chosen for Acquisition. Agilent recommends providing enough disk space for one year of lab operation, in addition to the operating system and MassHunter Workstation requirements.
The MassHunter Workstation is available either with storage in the local file system (MassHunter Workstation) or on a remote OpenLab Server or OpenLab ECM XT server with built-in Content Management database (MassHunter Networked Workstation).
Note that on Networked Workstations, data is only temporarily stored in a secured location on the local computer until it is transferred to the OpenLab Server/ECM XT server.
PC Recommendation
Table 2 provides the recommended hardware configuration for MassHunter computers with a single LC/TOF or LC/Q-TOF instrument.
Table 1. Tested and recommended hardware configuration for Workstations and Networked Workstations for TOF/Q-TOF
| Item | For all LC/Q-TOF except 6546 | For 6546 only |
|---|---|---|
| Description | Standard MassHunter-ready Computer | High Capacity MassHunter-ready Computer |
| Processor speed (CPU) | Intel Xeon W-2123, 4 core, 3.6 GHz | Intel Xeon W-2235, 6 core, 3.8 GHz |
| Physical memory (RAM) | 32 GB | 64 GB |
| Hard disk | 1 TB M.2 NVMe SSD - Primary (C:\) Boot. 4 TB x 2 RAID1 (4TB) - Data (D:\) | 1 TB M.2 NVMe SSD - Primary (C:\) Boot. 6 TB × 4 RAID10 (12 TB) - Data (D:\) |
| Graphic Resolution | 1920 x 1080 | 1920 x 1080 |
| USB port* | 1 USB port required for installation | 1 USB port required for installation |
| LAN card - House LAN card - instrument+ | Integrated Intel I217LM PCIe GbE Controller Integrated Intel I217LM PCIe GbE Controller | 1 Integrated Intel I217LM PCIe GbE Controller 1 Intel Ethernet 210-T1 PCIe |
* If a USB port is not available, the installation media can be copied over the network or downloaded from https://agilent.subscribenet.com.
+ A second LAN interface is required to isolate the instrument's data traffic from the local area network.
Existing MassHunter Workstations with the Agilent bundled Z4 G4 PC are supported with MassHunter Workstation 12.0 running in Workstation configuration only.
Table 2. Minimum hardware configuration for Workstations
| Item | For All TQ systems |
|---|---|
| Description | Hewlett-Packard Z4 G4 Minitower |
| Processor speed (CPU) | Intel Xeon W-2123 (3.6 GHz, 8.25 MB cache, 4 cores) |
| Physical memory (RAM) | 16GB (2x8GB) DDR4 2666 DIMM ECC Registered Memory |
| Hard disk | 2 x 500GB 7200 RPM SATA 6G Hard Drive (RAID 1) |
| Graphic Resolution | 1920 x 1080 |
| USB port* | 1 USB port required for installation |
| LAN card+ | 2 x Integrated Intel I219 and I210 PCIe GbE |
* If a USB port is not available, the installation media can be copied over the network or downloaded from https://agilent.subscribenet.com.
† A second LAN interface is required to isolate the instrument's data traffic from the local area network.
Windows 10 and Windows 11 Configuration
When setting up a MassHunter computer for the first time, some actions and settings are required. The MassHunter installer configures Windows 10 with required and performance settings.
These instructions show Windows 10. The changes apply to Windows 11 as well.
Required Actions
These steps must be taken for a new Windows installation, before the software is installed.
- In Control Panel > System, activate Windows with Microsoft.
Do this step only if the computer is customer-supplied. Computers from Agilent are already activated. - In Windows Settings > Update and Security, click Check for updates to check for updates and apply all patches.
Make sure all updates are downloaded and installed before you proceed.
Make sure that no reboot is pending.
CAUTION
Windows Update must not have a pending installation during installation of MassHunter Workstation.
Required Settings
Windows Control Panel Options
Power Options
- Preferred plan = High performance.
- Put the computer to sleep = Never.
- Change advanced power settings > Hard disk > Turn off hard disk after > Minutes = 0 (never).
Description of Power Options Screenshot: Screenshot of Windows Power Options settings, showing 'High performance' plan selected, 'Turn off the display' set to 'Never', 'Put the computer to sleep' set to 'Never', and 'Turn off hard disk after' set to '0 minutes (never)' in advanced settings.
Administrative Tools
In Local Security Policy > Local Policies > Security Options:
- Network Access: Sharing and security model for local accounts = Classic - local users authenticate as themselves.
Date and Time
- Time zone of your computer's location.
Network and Sharing Center
In Change adapter settings > Local Area Connection > Properties > Configure > Power Management:
- All check boxes cleared.
Description of Network Adapter Properties Screenshot: Screenshot of network adapter properties, specifically the 'Power Management' tab, with checkboxes 'Allow the computer to turn off this device to save power' and 'Allow this device to wake the computer' visible. The instruction is to clear the 'Allow the computer to turn off this device to save power' checkbox.
Programs and Features
In Turn Windows features on or off:
- .NET Framework 3.5 (includes .NET 2.0 and 3.0) = selected
This option requires an Internet connection.
If this procedure does not work as expected, or the computer has no Internet access, install .NET 3.5 from the Windows installation media (see details for Windows 10 in https://support.microsoft.com/en-us/kb/2734782). If you do not have installation media, create them as described https://www.microsoft.com/en-us/software-download/windows10.
- .NET Framework 3.5 (includes .NET 2.0 and 3.0) > Windows Communication Foundation Non-HTTP Activation = selected
This setting makes sure that the net.tcp components are properly initialized, enable Non-HTTP activation
- .NET Framework 4.6 Advanced Services = selected.
- Telnet Client = selected.
- TFTP Client = selected.
The system must be rebooted after Windows features are turned on.
Windows Settings
System > Tablet Mode
- When I sign in: Use desktop mode
Description of Tablet Mode Screenshot: Screenshot of Windows Settings for 'Tablet mode' showing 'Never use tablet mode' selected.
Apps > Offline Maps
- Metered connections = Off
- Map updates = Off
Description of Offline Maps Screenshot: Screenshot of Windows Settings for 'Offline maps' settings showing 'Metered connections' and 'Map updates' turned off.
Required Windows Explorer options
This Windows Explorer option is required.
View > Navigation pane
- Navigation Pane = selected.
Performance Options
Control Panel
These Control Panel options enhance your system performance.
In Advanced > Performance > Visual Effects:
- Adjust for best performance = selected.
- For Custom, these are selected for better usability:
- Smooth edges of screen fonts
- Show shadows under mouse pointer
- Show shadows under windows
In Advanced > Performance > Advanced > Virtual Memory:
- Paging file size for each drive > Custom Size > Maximum Size = 2 to 3 times the physical RAM on the PC. If available, use a drive different from the system installation drive to create the paging file.
In Advanced > Performance > Advanced > Data Execution Prevention:
- Turn on DEP for essential Windows programs and services only = selected.
Windows Settings
Personalization > Colors
- Transparency Effects = Off
2 Network Requirements
This chapter describes the network requirements that must be met in order to support the environmental computing needs of a MassHunter system.
Introduction
MassHunter systems rely on network infrastructure in order to support the communication between Networked Workstations and the OpenLab Server/ECM XT server. This communication is based on standard TCP/IP protocols. In order to provide optimum performance and uptime, the network must meet design criteria for available bandwidth, IP address assignment, name resolution and appropriate isolation of the lab subnet from the corporate network.
Refer to the Agilent OpenLab Server and OpenLab ECM XT Hardware and Software Requirements Guide (p/n D0007295) for more details about networking requirements.
LAN Connectivity
All MassHunter Networked Workstations include at least two 1 GB Ethernet network interfaces. One is reserved for PC-to-Instrument communication. The other is used to communicate with the OpenLab Server / ECM XT / ECM 3.x.
Note these LAN Connectivity requirements:
- For the 6546: a 10 GB Ethernet adapter is required for PC-to-instrument communication. In this configuration, high quality Cat 6 Ethernet over twisted-pair cables or better are required.
- NIC teaming¹: LAN cards should not be teamed on workstations, instrument controllers, or clients.
- Communication between the Networked Workstation and OpenLab Server/ECM XT must be on the same subnet. 1 GB Ethernet or greater connection is required.
- For TOF/Q-TOF instruments only: The OpenLab Server/ECM XT server/ECM 3.x server must be located on the same physical premises as the Networked Workstation. Co-located or off-premise/cloud-based servers are not supported.
- USB network adapters are not supported.
¹ Network Interface Card (NIC) teaming is also known as Load Balancing and Failover (LBFO)
LAN Power Management
Avoid data capture or transfer interruptions in your data acquisition system by making LAN communication cards available for instrument and system component communications.
Windows may be set to turn instruments/components off to save power while sleeping or hibernating. To change this setting:
- In the Microsoft Control Panel, open the Network and Sharing Center.
- Select Change adapter settings. Right-click Local Area Connection, and then click Properties > Configure.
- Select the Power Management tab.
- Clear the Allow the computer to turn off this device to save power check box.
Depending on the model of network adapter, the name of this option can also be Energy efficient Ethernet, Power saving Ethernet mode, or a similar name.
CAUTION
While applying Windows Updates, LAN Power Management might become reactivated. Be sure to check the LAN Power Management settings after all Windows Updates.
Firewall Settings
If you are using a third party firewall or anti-virus software on the network where MassHunter is installed, open these firewall ports to allow communication between the system components of MassHunter. These apply to workstations as well as to Client/Server systems as component communications rely on these communication channels:
Table 3. Firewall ports
| Ports | Comment, required for |
|---|---|
| 6570 | SubscribeNet: active retrieval and release of product licenses |
| 8084 | Agilent OpenLab Licensing support |
| 8085-8089 | Alternative to port 8084 if that port is in use by another page or process |
| 8090 | Hosts the viewing page of current license grants and consumptions found in the OpenLab Control Panel administration interface |
| 27000-27009 | Communication of license availability |
| 30101 | Store and retrieve instrument data |
| 53971 | Activity log messages display notification |
The MassHunter Workstation installer will automatically open required ports on an enabled Windows firewall during installation.
You will need to disable Windows Defender Firewall when you update the firmware.
Domain Requirements
Domains support the flow of information and user access rights across machines in the network. This means that all machines and instruments within the MassHunter Networked Workstation system must reside within the same domain or have the appropriate cross domain trusts to allow name based communications between all components in the system. In the case of a workstation installation, domains are only relevant if you are using a Windows domain-based authentication model. In this case the workstation or client must always be able to communicate with domain components in order to function as expected.
Installing MassHunter Workstation will apply network exceptions to the Windows firewall under the domain profile to result in a functional system. The components necessary to support MassHunter on a domain are:
- Domain controller – broadcasts the domain name and negotiates access to machines.
- Domain name server (DNS) – maintains records of what host names belong to which IP on the network. This component is always required for effective components communications in networked systems.
- Active directory – maintains the list of users and their access rights on the domain.
The domain name server (DNS) must be able to resolve the IPv4 address of all instrument controllers and instruments. Any unresolved instrument controller or instrument will disrupt the functionality of MassHunter resulting in errors or delays. IPv6 is not supported and must be deactivated.
MassHunter Workstation components must not be installed on the same machine as the domain controller.
The domain components above host a variety of services and settings that must be configured appropriately to allow communication across machines. The following services and settings will need to be configured to fit your domain. Your internal IT group is responsible for proper configuration of any custom domain solutions. These include settings for:
- Lookup zones and hostnames
- Group and security policies
- Subnet masks and Virtual LANs
- IP reservation (static or DHCP)
Environments with Proxy Servers
The OpenLab server must be accessible via http or https in the network. If you use proxy servers, verify that they can be accessed. If required, adjust the proxy settings.
Network Isolation
MassHunter Networked Workstations must be isolated from network environments that experience frequent failures due to faulty switching, viruses, or worms. If network isolation is not possible, the machines should be reconfigured and disconnected from the problematic network until these issues can be resolved. On an isolated network, name resolution services must be hosted by a separate machine to enable proper communications between system components by name.
An isolated network is completely physically isolated, so that no LAN switch connections on the network are shared with the corporate network infrastructure. Description of Figure 3. Sample client/server topology: Network Isolation: A diagram depicting a physically isolated client/server network topology. It shows multiple client workstations, each connected to analytical instruments, and all clients connected to a central server, with no shared LAN switch connections to a corporate network.
Specific Requirements for Compliant Systems
If you intend to use your system in a compliant environment, ensure the following settings related to time synchronization:
- Your network must have a time synchronization service to make sure that all systems are using a consistent and valid time.
- To ensure that users cannot change the time on a client system, users must not operate using an administrator account. This is important as the client time is used during buffered activity logging during network outages.
3 Instrument Connections
This chapter provides information on the instruments supported by the current revision of OpenLab CDS and the required respective instrument drivers and firmware revisions.
RC.NET Drivers and OpenLab CDS
MassHunter can control instruments and modules that use RC.NET based driver software only.
Agilent and other vendors may release RC.net drivers independent of the MassHunter releases. Agilent recommends always using the most recent firmware revisions which include latest firmware features and improvements. Agilent driver software is forward compatible with respect to firmware, i.e. the firmware can be updated without the need of updating the driver or CDS.
More information on instrument drivers and firmware is available in the respective RC.net driver release notes.
The following Agilent instrument driver software revisions are part of the MassHunter Acquisition software package, and are installed by default with the software:
Table 4. Agilent driver packages shipped and installed with MassHunter 12.0
| RC.net Instrument Driver | Driver Software Revision |
|---|---|
| Agilent LC | 3.5 |
| CTC PAL XT | B.01.08 |
| Agilent TQ | 3.0 |
| Agilent TOF/Q-TOF | Not an RC.net driver |
Additional RC.NET instrument drivers are supported with MassHunter 12.0 and will need to be installed separately.
Third Party Drivers
MassHunter Acquisition for 6400 Series Triple Quadrupole LC/MS or MassHunter Acquisition for Ultivo LC/TQ does not support third party instruments.
Agilent LC
Most Agilent LC Modules can be controlled with the current version of MassHunter Workstation. LC driver 3.5 has been tested with this revision and is installed by default with the software.
Recommended Firmware
Agilent recommends using current LC firmware sets with your MassHunter Workstation. The latest LC firmware sets are 7.xx. See Table 5.
Firmware Set 7.xx can be downloaded from http://www.agilent.com/en-us/firmwareDownload?whid=99818.
LC and CE firmware revisions are grouped into sets for each module or system. Firmware sets include just the latest firmware of each module.
Modules combined into one LC instrument always need to have firmware from the same set. Firmware of one set is fully compatible with your CDS that supports this firmware set. However, if one module uses a firmware revision lower that the specified Minimum Firmware Revision some functionality might not be supported.
Do not mix firmware revisions from one set with older or newer sets. Firmware is not tested across set borders!
Table 5. Recommended firmware
| Device | Recommended Firmware |
|---|---|
| Agilent 1100 Series, 1200 Series and 1200 Infinity | B.07.30 or later |
| Agilent 1200 Series, 1200 Infinity and 1120 Compact LC | B.07.30 or later |
| Agilent 1200 Infinity Hosted Modules | C.07.20 or later |
| Agilent 1260/1290 Infinity II Modules | D.07.33 or later |
You need to upgrade all existing modules to the latest version only if
- you add a new LC module to the existing system (recommended).
- one of the existing modules requires an upgrade due to a bug fix solved with the latest release.
For more information on downloading the current LC firmware, please refer to the Agilent website at http://www.agilent.com/en-us/firmwareDownload?whid=69761 (LC Firmware News and Downloads) and http://www.agilent.com/en-us/firmwareDownload?whid=83974 (Firmware Update Tools & Procedures).
The tables that follow list the supported LC modules with the minimum required firmware. Please note that using some driver features like valve-thermostat clusters and new temperature control modes require firmware updates beyond the minimum required firmware for related modules. For more information please refer to the respective release notes.
Table 6. 1120 Compact LC / 1220 Infinity LC
| 1120 Compact LC / 1220 Infinity LC | Version |
|---|---|
| G4286A Compact LC | B.07.30 [005] |
| G4286B Infinity LC | B.07.30 [005] |
| G4286C Infinity LC | B.07.30 [005] |
| G4287A Compact LC | B.07.30 [005] |
| G4287B Infinity LC | B.07.30 [005] |
| G4288A Compact LC | B.07.30 [005] |
| G4288B Infinity LC | B.07.30 [005] |
| G4288C Infinity LC VL | B.07.30 [005] |
| G4289A Compact LC | B.07.30 [005] |
| G4289B Infinity LC | B.07.30 [005] |
| G4289C Infinity LC VL | B.07.30 [005] |
| G4290A Compact LC | B.07.30 [005] |
| G4290B Infinity LC | B.07.30 [005] |
| G4290C Infinity LC VL | B.07.30 [005] |
| G4291A Compact LC | B.07.30 [005] |
| G4291B Infinity LC | B.07.30 [005] |
| G4291C Infinity LC VL | B.07.30 [005] |
| G4292A Compact LC | B.07.30 [005] |
| G4292B Infinity LC | B.07.30 [005] |
| G4292C Infinity LC VL | B.07.30 [005] |
| G4293A Compact LC | B.07.30 [005] |
| G4293B Infinity LC | B.07.30 [005] |
| G4293C Infinity LC VL | B.07.30 [005] |
| G4294B Infinity LC VL | B.07.30 [005] |
| G4294C Infinity LC VL | B.07.30 [005] |
Table 7. 1260 Infinity LC
| 1260 Infinity LC | Version |
|---|---|
| G1156A 6-Position/7-Ports Valve | A.07.01 [001] |
| G1157A 2-Position/10-Ports Valve | A.07.01 [001] |
| G1158A 2-Position/6-Ports Valve (1100/1200) | A.07.01 [001] |
| G1158B 2-Position/6-Ports Valve | A.07.01 [001] |
| G1159A 6-Position Column Selector Valve | A.07.01 [001] |
| G1160A 12-Position Selector Valve | A.07.01 [001] |
| G1162A Micro Valve 2-Position/6-Ports | A.07.01 [001] |
| G1163A Micro Valve 2-Position/10-Ports | A.07.01 [001] |
| G1310A Isocratic Pump (1100/1200) | A.07.01 [001] |
| G1310B Isocratic Pump | A.07.01 [001] |
| G1311A Quaternary Pump (1100/1200) | A.07.01 [001] |
| G1311B Quaternary Pump | A.07.01 [001] |
| G1311C Quaternary Pump VL | A.07.01 [001] |
| G1312A Binary Pump (1100/1200) | A.07.01 [001] |
| G1312B Binary Pump | A.07.01 [001] |
| G1312C Binary Pump VL | A.07.01 [001] |
| G4302A SFC-Binary Pump | A.07.01 [001] |
| G1313A Autosampler (1100) | A.07.02 [005] |
| G1314A Variable Wavelength Detector (1100/1200) | A.07.02 [001] |
| G1314B Variable Wavelength Detector VL | A.07.02 [001] |
| G1314C Variable Wavelength Detector VL+ | A.07.02 [001] |
| G1314D Variable Wavelength Detector (1200) | B.07.33 [003] |
| G1314F Variable Wavelength Detector | B.07.33 [003] |
| G1315A Diode Array Detector (1100/1200) | A.07.02 [001] |
| G1315B Diode Array Detector (1100/1200) | A.07.02 [001] |
| G1315C Diode Array Detector VL+ | B.07.33 [003] |
| G1315D Diode Array Detector VL | B.07.33 [003] |
| G1316A Thermostatted Column Compartment | A.07.02 [001] |
| G1316B Thermostatted Column Compartment SL (1200) | A.07.02 [001] |
| G1321A Fluorescence Detector (1100/1200) | A.07.02 [001] |
| G1321B Fluorescence Detector SPECTRA | A.07.02 [001] |
| G1321C Fluorescence Detector | A.07.02 [001] |
| G1329A Standard Autosampler (1100/1200) | A.07.02 [005] |
| G1329B Standard Autosampler | A.07.02 [005] |
| G1365A Multiple Wavelength Detector (1100/1200) | A.07.02 [001] |
| G1365B Multiple Wavelength Detector (1100/1200) | A.07.02 [001] |
| G1365C Multiple Wavelength Detector | B.07.33 [003] |
| G1365D Multiple Wavelength Detector VL | B.07.33 [003] |
| G1367A Well Plate Sampler (1100) | A.07.02 [005] |
| G1367B High Performance Autosampler (1200) | A.07.02 [005] |
| G1367C High Performance Autosampler SL (1200) | A.07.02 [005] |
| G1367D High Performance Autosampler SL+ (1200) | A.07.02 [005] |
| G1367E High Performance Autosampler | A.07.02 [005] |
| G1369C LAN Interface Card | B.07.33 [003] |
| G1376A Capillary Pump | A.07.01 [001] |
| G1377A Micro Well Plate Sampler | A.07.02 [005] |
Table 8. 1290 Infinity LC
| 1290 Infinity LC | Version |
|---|---|
| G1170A Universal Valve Drive* | C.07.30 [001] |
| G1314E Variable Wavelength Detector | B.07.33 [003] |
| G1316C Thermostatted Column Compartment+ | A.07.02 [001] |
| G4204A Quaternary Pump | B.07.33 [003] |
| G4220A Binary Pump | B.07.33 [003] |
| G4212A Diode Array Detector | B.07.33 [003] |
| G4226A Autosampler | A.07.02 [005] |
| G4227A Flexible Cube | C.07.30 [001] |
* A host may be required. For details see latest driver release note.
+ Cluster with up to three G1316C with integrated 8pos/9port valves (products G4230A/B). Minimum two G1316C TCCs, the third TCC can be a G1316A, B or C.
Table 9. 1260/1290 Infinity II LC
| 1260/1290 Infinity II LC | Version |
|---|---|
| G4782A Binary Pump SFC | D.07.33 [003] |
| G4767A Multisampler SFC | D.07.33 [003] |
| G5654A Bio-inert Quaternary Pump | D.07.33 [003] |
| G5668A Bio-inert Multisampler | D.07.33 [003] |
| G7104A Quaternary Pump | B.07.33 [003] |
| G7104C Flexible Pump | B.07.33 [003] |
| G7110B Isocratic Pump | D.07.33 [003] |
| G7111A Quaternary Pump VL | D.07.33 [003] |
| G7111B Quaternary Pump | D.07.33 [003] |
| G7112B Binary Pump | D.07.33 [003] |
| G7114A Variable Wavelength Detector | D.07.33 [003] |
| G7114B Variable Wavelength Detector | D.07.33 [003] |
| G7115A Diode Array Detector | D.07.33 [003] |
| G7116A Multicolumn Thermostat | C.07.30 [001] |
| G7116B Multicolumn Thermostat | C.07.30 [001] |
| G7117A Diode Array Detector FS | D.07.33 [003] |
| G7117B Diode Array Detector | D.07.33 [003] |
| G7117C Diode Array Detector HS | D.07.33 [003] |
| G7120A Binary Pump | B.07.33 [003] |
| G7121A Fluorescence Detector | D.07.33 [003] |
| G7121B Fluorescence Detector SPECTRA | D.07.33 [003] |
| G7129A Vialsampler | D.07.33 [003] |
| G7129B Vialsampler | D.07.33 [003] |
| G7129C Vialsampler | D.07.33 [003] |
| G7132A Bio Binary Pump | D.07.33 [003] |
| G7137A Bio Multisampler | D.07.33 [003] |
| G7165A Multiple Wavelength Detector | D.07.33 [003] |
| G7167A Multisampler | D.07.33 [003] |
| G7167B Multisampler | D.07.33 [003] |
Table 10. Capillary Electrophoresis (CE)
| Module | Version |
|---|---|
| G7100A Capillary Electrophoresis (CE) | B.07.30 [001] |
Table 11. Other modules
| Product Number | Module Name | Minimum Firmware Revision |
|---|---|---|
| G1330A | 1100 Series Thermostat | n/a |
| G4277A | 1290 Infinity LC Injector HTS | 4.3.0 |
| G1330B | 1290 Infinity Thermostat | n/a |
| G4270-CTC | HTC PAL Auto sampler | Agilent 2 6.8 or 4.1.0 |
| G4271-CTC | HTS PAL Auto sampler | Agilent 2 6.8 or 4.1.5 |
| G7130A | Integrated Column Compartment ICC | D.06.76 |
| VTC Valve Thermostat Cluster | Combinations of G7116B, G1170A and G1316C (valve or column hosts) and G1316A/B and G7130A | See LC Driver Release Notes: Valve-Thermostat Cluster |
| HDR-DAD Cluster | 2x G4212A, 2x G4212B, 2x G7117A or 2x G7117B, or G7117A and 1x G7117B | B.06.57 |
Agilent LC/MS
Recommended Firmware
Always use the most recent Firmware or Embedded Software installation package that comes with the driver package.
Triple Quad LC/MS System
These Agilent LC/MS instruments can be controlled with MassHunter Workstation.
Table 12. 6400 Series Triple Quad LC/MS System
| Product number | Description | Supported source | Autotune source | Checktune source |
|---|---|---|---|---|
| G6420A | 6420 LC/TQ | ESI | ESI | ESI |
| G6460C | 6460C LC/TQ | AJS, ESI | AJS, ESI | AJS, ESI |
| G6470A | 6470 LC/TQ | AJS, ESI, APCI, MMI, APPI, ChipCube | AJS, ESI, APCI, MMI | AJS, ESI, APCI (6470 A/B only), MMI |
| G6470B | 6470 LC/TQ | AJS, ESI, APCI, MMI, APPI, ChipCube | AJS, ESI, APCI, MMI | AJS, ESI, APCI (6470 A/B only), MMI |
| G6475A | 6475 LC/TQ | AJS, ESI, APCI, MMI, Nano ESI | AJS, ESI, APCI, MMI | AJS, ESI, APCI, MMI |
| G6495A | 6495A LC/TQ | AJS, ESI, APCI, MMI, APPI, ChipCube | AJS, ESI, APCI, MMI | AJS, ESI, APCI (6495C only), MMI |
| G6495B | 6495B LC/TQ | AJS, ESI, APCI, MMI, APPI, ChipCube | AJS, ESI, APCI, MMI | AJS, ESI, APCI (6495C only), MMI |
| G6495C | 6495C LC/TQ | AJS, ESI, APCI, MMI, APPI, ChipCube | AJS, ESI, APCI, MMI | AJS, ESI, APCI (6495C only), MMI |
Table 13. 6400 Series Triple Quad LC/MS System
| Product number | Description | Supported source | Autotune source | Checktune source |
|---|---|---|---|---|
| G6465A | Ultivo LC/TQ | AJS, ESI, APCI, MMI | AJS, ESI, APCI, MMI | AJS, ESI, APCI, MMI |
| G6465B | Ultivo LC/TQ | AJS, ESI, APCI, MMI | AJS, ESI, APCI, MMI | AJS, ESI, APCI, MMI |
LC/TOF and LC/Q-TOF System
These Agilent LC/TOF and LC/Q-TOF instruments can be controlled with MassHunter Workstation.
Table 14. Agilent LC/TOF Systems
| Product number | Description | Note |
|---|---|---|
| G6224A | 6224A Accurate-Mass TOF LC/MS | No support for AJS or Dual AJS Source |
| G6230A | 6230A Accurate-Mass TOF LC/MS | |
| G6230B | 6230B Accurate-Mass TOF LC/MS |
Table 15. Agilent LC/Q-TOF Systems
| Product number | Description | Note |
|---|---|---|
| G6520B | 6520B Accurate-Mass Q-TOF LC/MS | No support for AJS or Dual AJS Source |
| G6530A | 6530A Accurate-Mass Q-TOF LC/MS | |
| G6530B | 6530B Accurate-Mass Q-TOF LC/MS | |
| G6530C | 6530C Accurate-Mass Q-TOF LC/MS | |
| G6538A | 6538 Ultra High Definition (UHD) Accurate-Mass Q-TOF LC/MS | No support for AJS or Dual AJS Source |
| G6540A | 6540 Ultra High Definition (UHD) Accurate-Mass Q-TOF LC/MS | |
| G6540B | 6540 Ultra High Definition (UHD) Accurate-Mass Q-TOF LC/MS | |
| G6545A | 6545A LC/Q-TOF | |
| G6545B | 6545B LC/Q-TOF | |
| G6546A | 6546 LC/Q-TOF | |
| G6549A | 6545XT AdvanceBio LC/Q-TOF | |
| G6550A | 6550 iFunnel Q-TOF LC/MS System | |
| G6550B | 6550B iFunnel Q-TOF LC/MS System | |
| G6560A | 6560 Ion Mobility LC/Q-TOF | |
| G6560B | 6560 Ion Mobility LC/Q-TOF |
Drivers for non-Agilent Instruments
If any drivers are available for non-Agilent instruments, they will be available from SubscribeNet
Incompatible Instruments and Modules
The following tables summarize selected instruments or modules that can not be controlled with the current revision of MassHunter Workstation.
Incompatible LC and LC/MS Modules
Table 16. Incompatible LC and LC/MS Modules
| Product Number | Module Name |
|---|---|
| G1361A | 1260 Infinity Preparative Pump |
| G1364A/B/C/D | Fraction Collector / Fraction Cluster |
| G1364E | Fraction Collector (Prep) |
| G1364F | Fraction Collector (Analytical) |
| G1389A | Micro Sampler |
| G2258A | 1260 Infinity Dual-Loop Autosampler |
| G2260A | 1260 Infinity Preparative Autosampler (High flow) |
| G4218A, G7102A, G4260A, G4260B, G4261A, G4261B | Evaporative Light Scattering Detector (ELSD) |
| G5664A | 1260 Infinity Bio-inert fraction collector AS |
| G5664B | Fraction Collector (BIO) |
| G7157A | 1260 Infinity II Preparative Autosampler |
| G7158B | Preparative Open Bed Sampler/Collector |
| G7159B | Preparative Open Bed Fraction Collector |
| G7161A | 1260 Infinity II Prep Binary Pump |
| G7161B | Binary Prep Pump |
| G7162A | Refractive Index Detector |
| G7162B | Refractive Index Detector Micro |
| G7166A | Valve-based Fraction Collector |
| G7170B | MS Flow Modulator |
Incompatible CE Instruments
MassHunter Workstation cannot be used to control CE Instruments.
4 Software Compatibility
This chapter contains information on compatibility with other Agilent or Non-Agilent Software.
Software
The MassHunter Workstation 12.0 software includes MassHunter Acquisition for LC/TQ 12.0, MassHunter Quantitative Analysis 12.0, and MassHunter Qualitative Analysis 10.0.
These programs are compatible with MassHunter Workstation 12.0:
- LabAdvisor for LC
- ACE
- Remote Advisor
- MassHunter WalkUp - TOF/Q-TOF only
- MassHunter IM-MS Browser - TOF/Q-TOF only
- MassHunter BioConfirm 12.0 - TOF/Q-TOF only
- MassProfiler Professional (MPP)
- RapidFire
- MOBILion Software¹ - TOF/Q-TOF only
¹ In a MOBILion MOBIE system, MOBIE EyeOn Software is used to acquire data in HRIM mode. MassHunter Data Acquisition is used to acquire data in Q-TOF mode.
Libraries and Databases
The following libraries and databases are compatible with MassHunter Workstation.
- G4975BA NIST 20 LC/MS/MS Library (includes NIST MS Search)
- G3878CA MassHunter Pesticide PCDL B.07.00 or higher
- G3876CA MassHunter Forensic/Toxicology PCDL B.07.00 or higher
- G3879CA MassHunter Veterinary Drugs PCDL B.07.00 or higher
- G6890CA MassHunter Extractables and Leachables PCDL B.07.00 or higher
- G5883CA MassHunter Mycotoxin and related Metabolites PCDL B.07.00 or higher
- G6882CA MassHunter Water Screening PCDL B.07.00 or higher
- G3874AA METLIN Metabolites PCDL
- G6825AA Personal METLIN Metabolite PCD
- Any user-created library
- G1733CA Pesticides tMRM DB
- G1734CA Forensics tMRM DB
- G1735CA Vet drug tMRM DB
- G1736AA PFAS MRM DB for LC/TQ
- G6412AA Metabolomics MRM DB
