User Guide for GALENVS models including: UPX0100 Universal Pathogen Complex Module, UPX0100, Universal Pathogen Complex Module, Pathogen Complex Module, Complex Module
Animal Diagnostics – Galenvs
Guides & Protocols – Galenvs
Universal Pathogen Complex Matrix Module Quick Start Guide For milk and fecal samples, proceed to step A For all other matrices, eg. oral fluid, viscous, swab, stool, exudate, urine, etc., proceed to step B Milk Fecal A To the 2mL Bead Beating tube provided, add 200µL of sample, 300µL Pre-Treatment Buffer (PTB) and 40µL Proteinase K. Shake well before use. Vortex for 30 seconds and heat at 70°C for 5 mins. To pellet debris and solids, briefly centrifuge at 13000rpm for 1 min. Note: use Pre-Treatment Beads (small) for milk and Pre-Treatment Beads (large) for fecal samples. Sample PTB Proteinase K 70°C 13,000 RPM Universal Pathogen Complex Matrix Module Other matrices B To a 1.5mL centrifuge tube, add 200µL of sample, 300µL Pre-Treatment Buffer (PTB) and 40µL Proteinase K. Shake well before use. Vortex for 30 seconds and heat at 70°C for 5 mins. To pellet debris and solids, briefly centrifuge at 13000rpm for 1 min. Collect 300µL and proceed to Universal Pathogen DNA/RNA Extraction Kit Sample PTB Proteinase K 1.2 70°C 13,000 RPM Manual: Automated:Adobe PDF library 17.00 Adobe Illustrator(R) 24.0