Instruction Manual for Vabiooth models including: VB-M004, VB-M004 Professional Microscope, Professional Microscope, Microscope
Vabiooth Lab Compound Trinocular Microscope 40X-2500X Magnification, Two-Layer Mechanical Stage, Wide-Field 10X and 25X Eyepieces, Abbe Condenser Camera-Compatible for Pet Hospitals, Lab, Farms : Electronics
PROFESSIONAL MICROSCOPE VB-M004 VB-M005 Operation Manual This operation manual is for the Model VB-M004 VB-M005 microscope. We recommend that you carefully review this manual prior to operating this device in order to optimize safety and performance, and to become fully familiar with its use. Keep this operation manual in an easily accessible place for future reference. WARNING: To prevent fire and electrical shock, do not expose unit to rain or moisture. CAUTION: No user-serviceable parts inside. Refer servicing to qualified service personnel. Contents 1. Before use 3 2. Diagram 4 3. Assembly 5 4. Operation 5 5. Specification 8 6. Troubleshooting 9 7. Warranty 11 2 1. Before use 1-1. Operation (1) As the microscope is a precision instrument, always use it with care. Avoid dropping or shaking during operation. Make sure the microscope is placed on a flat surface. (2) Do not put the microscope in direct contact with the sun. Do not expose the microscope to high temperature, damp, or dusty environment. (3) When moving the microscope, you should use both hands, typically one hand under the base and one hand on the arm (stand) of the microscope and set it down carefully. CAUTION: It will damage the microscope if you hold the microscope either by the stage, focusing knob or head. (4) Be sure the microscope is unplugged before replacing the bulb or fuse and wait until the bulb has cooled down. (5) This microscope has a built in voltage transformer and can be used with wide voltage range from 110 to 240V. (6) Only use the power cord supplied with the microscope. 1-2 Maintenance (1) Wipe the lens gently with a soft lens tissue. After every use with oil or fingerprints clean the lens surfaces with lens paper or lens paper moistened with a little rubbing alcohol. If you do not have lens paper make sure to use a lint free towel or cloth. CAUTION: Alcohol is flammable. Do not place this chemical near fire or fire causing source. You should use this chemical in a ventilated place. (2) Don't use organic solution to wipe the surfaces of the other components. (3) If the microscope comes in contact with a liquid, then you should turn off the power immediately and wipe it dry. (4) Never disassemble or service the microscope yourself. (5) After using, cover the microscope with a dust cover. 3 2. Diagram Microscope Diagram 4 3. Assembly 3-1 Install the Objectives Install the objective into the microscope nosepiece from the lowest magnification to the highest, in a clockwise direction. 3-2 Insert the Eyepieces (1) Remove the eyepiece tube covers. (2) Insert the eyepiece into the tube completely. 3-3 Install plug Insert one side of the plug into the back socket of the microscope. 4. Operation Fig.1 4-1 Turn on (1) Insert the plug into an outlet and turn on. (2) Adjust the light adjustment knob until you get the suitable brightness. (See fig.1) 4-2 Set the specimen slide (1) Push the arm of the specimen holder back. (2) Insert the slide with the cover glass facing up and allow the arm of the specimen holder forward. (See fig.2) (3) Rotate the X and Y-axis knobs on the mechanical stage to move the specimen to the center (the center of the objective). 5 4.Operation Tension control knob Course Focus knob Fine focus knob 4-3 Adjust focus (1) Move the objective 4X into the light path. (2) Rotate the coarse-focusing-knob until the image appears. (3) Turn the fine-focusing-knob to make the specimen clearer. (See fig.3) · When using the 4X and 10X objective, open the aperture diaphragm and field Fig.3 . diaphragm to the widest position. Tighten Fig.4 Eye distance indicate board Fig.5 Rotate Diopters 4-4 Adjust tension of the focus If the course focus knob is difficult to turn when focusing or the stage drifts downwards by itself, you can solve the problem by adjusting the tension adjustment ring. Turn clockwise to tighten the controls or counterclockwise to loosen the controls (See fig.4) 4-5 Adjust the interpupillary distance When observing with both eyes hold the bases of the base of the eyepieces and rotate them around the axis until there is only one field of view. (See fig.5). On the left and right eyepiece base should point to the same number. The number is the interpupillary distance. 4-6 Adjust diopters Rotate one eyepiece to the "0" position using the white thread. This should be done to your dominate eye, then using that same (one side only) eyepiece focus on an specimen clearly. Now observe the other eyepiece and rotate its diopter adjusting ring until the image is clear in that eyepiece. (See fig.6) Fig.6 6 4. Operation Outside edge of objective Fig.7 Fig.8 Switch to open and close condenser 4-7 Adjust diaphragm The diaphragm can prevent other light from entering and strengthen the image by limiting the beam of light entering the condenser. When the image of the diaphragm is just on the edge of the field of view, you can obtain the clearest image. 4-8 Adjust aperture diaphragm The aperture diaphragm decides the numerical aperture of the illumination. If the N.A. of illumination is matching with N.A. of the objective, you can obtain better resolution and the contrast. If the contrast is low, you can adjust the N.A. of the condenser to 70%-80% of the N.A. of the objective when using. Adjust the upper diaphragm ring until you see the image like above (See fig.7). Use of diaphragm and put the N.A. of condenser to 80% of the objective. e.g.: objective 40X (N.A. =0.65), put the aperture diaphragm to about 0.65X0.8=0.52. (See fig.8) 4-9 Use the oil objective (1) Use the objective 40X to focus the specimen. (2) Put a drop of oil on the specimen. (See fig.9) (3) Rotate the nosepiece to place the 100X objective (oil) into the light path. Then use the fine focusing knob to focus. Make sure there is no air bubble in the oil. Oil position Fig.9 A. Take out the eyepieces to examine the air bubble. Open the aperture diaphragm and field diaphragm fully and observe the edge of the objective from the tube without the eyepiece. B. You can rotate nosepiece slightly and swing the oil objective some times to remove the air bubble. (4) After using oil, clean the 100X lens with clean lens paper using rubbing alcohol if the oil does not come off easily. 7 5. Specification 5-1 Microscope general specifications 8 6. Troubleshooting Symptom Cause Remedy Optics (1)The lamp is bright, but the light in the field of view is not bright enough. (2)The side of the field of view is dark or not even. Field diaphragm is not large enough. Condenser is too low. Condenser is not centered. The nosepiece is not in the right position. Stain or dust has accumulated on the condenser, objective, eyepieces, and base lens. Condenser adjustment needed (see step 3-7) Enlarge the field diaphragm. Adjust the condenser. Center the condenser. Turn the nosepiece into the right position. Clean the lens. Center the condenser. (3)Stain or dust is observed in the field of view. Stains have accumulated on the specimen. Stains have accumulated on the lens. Clean the specimen. Clean the lens. (4)Unclear image No cover glass on the specimen slide. Add the cover glass. The cover glass is not standard. Use a standard cover glass with thickness 0.17mm. The cover glass faces down. Put the cover glass to face up. The immersion oil has accumulated on the dry objective. The immersion oil is not used for oil objective 100XR. Clean thoroughly. Use immersion oil. Air bubble in the immersion. Get rid of the air bubble. Use wrong immersion oil. Use correct one. The aperture is not opened correctly. Adjust the iris diaphragm. Stain or dust has accumulated on the lens in the inlet of the head. Clean the lens. The condenser is not in the right position. Adjust the condenser. (5)One side of the field of view is dark or the image moves while focusing. The specimen slide is not fixed. The nosepiece is not in the right position. Condenser is centered incorrectly. Fix with clips. Turn the nosepiece into the right position. Center the condenser. (6) The eyes feel tired easily. The right field of view doesn't superpose with the left. Interpupillary distance is wrong. Diopter adjustment is wrong. The eyepiece for the right is different from the left. Adjust the interpupillary distance Adjust the diopter. Use the same eyepieces. 9 6. Trouble shooting Symptom Mechanics Cause Remedy (1)Can not get the objective focused. (2) The objective touches the cover glass while turning the nosepiece. (3)Coarse focusing knob is too tight. (4)Stage declines itself and can't stay in the focus plane. (5)Coarse focusing knob can't rise. (6)Coarse focusing knob can't decline. The cover glass faces down. The cover glass is not standard. The cover glass faces down. The cover glass is not standard. Tension knob is too tight. Tension knob is too loose. The limit stop knob is locked. The base of the condenser is too low. Put the cover glass to face up. Use a standard cover glass with thickness 0.17mm. Put the cover glass to face up. Use a standard cover glass with thickness 0.17mm. Loosen a little. Tighten a little. Loosen the knob. Raise the base. (7)Can not move the slide smoothly. (8)The image moves obviously when touching the stage. Electrical The slide is not fixed correctly. Adjust it correctly. The movable specimen holder is not fixed properly. Adjust it correctly. The stage is fastened incorrectly. Fasten the stage correctly. (1)The bulb does not work. No power supply. The bulb is not inserted correctly. Check the connection of the power cable. Insert it correctly. The bulb burnt out. Replace it. (2)The bulb burnt out usually. Use a wrong bulb. Replace with a correct one. (3)The field of view is Use a wrong bulb. not bright enough. The use of light adjustment knob is wrong. Replace with a correct one. Adjust correctly. (4)The bulb flickers or the brightness is not stable. The bulb will burn out soon. The wire doesn't connect all right. Replace with a new one. Connect correctly. 10 11Microsoft Word 2016