QIAGEN QIAamplfier PCR Cyclers and Kits: A Smooth Path to Success
Discover high-performance PCR solutions from QIAGEN, combining QIAamplfier cyclers, AllTaq PCR kits, and QIAxcel Advanced for efficient and reliable molecular biology workflows.
Introduction to QIAGEN PCR Solutions
Enjoy success with high-performance QIAGEN PCR kits and instrumentation. The combination of QIAamplfier PCR cyclers, AllTaq PCR kits, and the QIAxcel Advanced capillary electrophoresis instrument offers a smooth path to success for your laboratory.
- Discover AllTaq for all your single amplifications, or Multiplex PCR kits for effortless multiplexing.
- Amplify with the QIAamplfier PCR cyclers and analyze all your amplicons in minutes with the QIAxcel Advanced system.
Key features include:
- Fast cycling: 30 cycles in less than 45 minutes. ⏱️
- Whisper quiet operation: low noise emission (45 dB max.). ?
- Linear Gradient Function: enabling precise temperature gradients. ?
- Robust design: engineered for robustness and reproducibility of results. ✔️
The QIAamplfier 96: Versatile System for Performance
The QIAamplfier 96 is a high-performance thermal cycler featuring a 96-well block and a gradient function, ideal for all your end-point PCR requirements.
- Easy-to-use interface: intuitive software with a 7-inch color touchscreen.
- Fast ramping: heating and cooling rates of up to 4°C/sec.
- High-performance smart lid: provides pressure control for highly reproducible results.
- Linear Gradient Tool: allows linear gradients from 20°C to 99°C (max. span 20°C, minimal increment of 0.1°C).
AllTaq PCR Kit: One Master Mix for All Targets
The AllTaq PCR kit is optimized for ultrafast amplification of any target, suitable for routine amplification as well as challenging, long, or high G-C content templates.
It offers a single protocol for all targets, streamlining workflows to just 45 minutes. It enables duplex PCR and amplification of GC-rich or long targets up to 9 kb. Guard-protected and hot-start chemistry ensures superior specificity, sensitivity, and outstanding room temperature stability, with visual pipetting control for monitoring successful procedures.
Example Amplification Results:
Gel electrophoresis results demonstrating successful amplification of PPIA (100 bp), HSP90AA1 (83 bp), and ILK (166 bp) targets using the AllTaq Master Mix Kit. Reactions were performed with varying template amounts (1 ng gDNA for PPIA, 3 ng gDNA for HSP90AA1, 10 ng gDNA for ILK) and annealing temperatures (50°C to 60°C). Analysis on the QIAxcel confirmed successful and specific amplification of all targets, with no optimization of annealing temperature required.
QIAGEN Multiplex PCR Kit: The Power of Multiplexing
Amplify all your targets in a single tube to save time, money, and DNA template without the need for protocol optimization. The QIAGEN Multiplex PCR Kit outperforms kits from other suppliers, ensuring efficient and specific simultaneous extension of all targets for 5-plex, 10-plex, 15-plex, or more.
The master mix includes HotStarTaq DNA Polymerase, a unique PCR buffer with the novel synthetic Factor MP, and optimized salt concentrations. This combination stabilizes specifically bound primers and enables efficient extension of all primers. Q-Solution, a novel additive, is also supplied for efficient amplification of "difficult" (e.g., GC-rich) templates.
Example Multiplex PCR Results:
QIAxcel Advanced analysis of 11 isolates tested with an 11-gene multiplex PCR. The PCR included major E. coli virulence genes (stx1, stx2, eae, ehxA) and E. coli O-antigens from serogroups O26, O45, O103, O111, O121, O145, and O157. Lane A12 contained a DNA ladder. Amplicon sizes were verified by sequencing.
Embrace Electrophoresis with QIAxcel Advanced
The QIAxcel Advanced system revolutionizes electrophoresis analysis with several key benefits:
- Fast Analysis: Analyze up to 12 samples in as little as three minutes. ⏱️
- High Throughput: Unattended analysis of up to 96 samples.
- High Resolution: Resolution down to 3–5 bp for fragments <500 bp.
- High Sensitivity: Reliable detection with sensitivity down to 0.1 ng/µl.
- Automated Sample Loading: Minimizes error-prone manual steps. ⚙️
- Standardized Analysis: Utilizes predefined protocols.
- Cost-Effective: Features reusable cartridges with optimized cost-efficiency.
- Advanced Software: Provides state-of-the-art sample analysis.
Workflow: From Amplification to Interpretation
QIAGEN's integrated solutions streamline your PCR workflow:
Process Flow: Assay setup (using AllTaq and Multiplex PCR kits) → Amplification (using QIAamplifier 96) → Detection (using QIAxcel Advanced System).
Hot Start Mechanism: The Guard molecule and the 2-phase hot start mechanism enhance target specificity by double-blocking the polymerase, preventing non-specific amplifications. Inactive DNA Polymerase is activated by heat (2 min, 95°C) to become Active DNA Polymerase.
Commitment to Research
QIAGEN is committed to accelerating your research by providing high-quality PCR solutions that meet all your needs. Choosing optimized AllTaq chemistries and a QIAamplifier cycler offers fast, easy, and powerful analysis for your PCR experiments, saving time, money, and providing peace of mind.
