Innovative Diagnostic Solutions for Hygiene & Food Safety | Hygiena
User Guide for hygiena models including: KIT230108 LP, KIT230109 RP, KIT230108 LP GMO Soya Identification 3 LyoKit, GMO Soya Identification 3 LyoKit, Soya Identification 3 LyoKit, Identification 3 LyoKit, 3 LyoKit
Identificação de OGMs | Hygiena
GMO Soya Identification 3 LyoKit | Hygiena
Identification des OGM | Hygiena
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DocumentDocumentfoodproof® GMO Soya Identification 3 LyoKit Ready Reference Guide Product No. KIT230108 (LP), KIT230109 (RP) Revision A, November 2023 PCR kit for the qualitative detection of FG72, A5547-127, DP-356043 and MON87705 DNA using real-time PCR instruments. Before starting, it is strongly recommended to read the entire product manual available on our website. PROGRAM SETUP Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels: u FAM (FG72), HEX (A5547-127), ROX (DP-356043) and Cy5 (MON87705). 95 °C 60 °C 37 °C 4 min | 10 min 5 sec | 60 sec* Pre-incubation: 1 cycle Step 1: 37 °C for 4 min Step 2: 95 °C for 10 min Amplification: 50 cycles Step 1 : 95 °C for 5 sec Step 2*: 60 °C for 60 sec 1 cycle 50 cycles * Fluorescence detection For some real-time PCR instruments the probe quencher as well as the use of a passive reference dye must be specified. This kit contains probes with a non-fluorescent "dark" quencher and no passive reference dye. A Color Compensation is necessary for users of the LightCycler® 480 System: Color Compensation Set 3 (Product No. KIT230005). DATA INTERPRETATION Verify results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results. Always compare samples to positive and negative controls. Review data from each channel and interpret results: If a signal is present in the channel _ the sample is positive for the respective target. Examples for data interpretation: FAM HEX ROX Cy5 Result Interpretation + - - - Positive for FG72 - + - - Positive for A5547-127 - - + - Positive for DP-356043 - - - + Positive for MON87705 + - + - Positive for FG72 and DP-356043 + + + - Positive for FG72, A5547-127 and DP-356043 - - - - Negative for all targets RR-KIT230108-09-RevA foodproof® is a registered trademark of Hygiena. The above mentioned real-time PCR instruments are registered trademarks of their respective holders. PREPARATION OF THE PCR MIX Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves. +25 µL 1. PLACE STRIPS IN RACK Take needed number of PCR tube strips out of aluminum bag. Important: close bag tightly afterwards. Place strips in a suitable PCR tube rack. If needed, gently tap the tubes to move the lyophilized pellets to the bottom of all tubes. 2. DECAP Immediately before filling, carefully open strips and discard caps. Do not leave open longer than necessary. 3. ADD SAMPLES AND CONTROLS Pipette 25 µL of samples, Negative Control (colorless cap) or Control Template (purple cap) into respective wells. If using less volume, add PCR-grade H2O to reach 25 µL. 4. SEAL Carefully seal the tubes with the provided 8-cap strips. 5. MIX Resuspend pellet after sealing by mixing thoroughly. Alternatively, resuspend pellet by pipetting up and down multiple times in Step 3. 6. CENTRIFUGE Briefly spin strips, e.g., 5 seconds at 500 - 1,000 x g, in a suitable centrifuge. 7. START REAL-TIME PCR RUN Cycle samples as described above. Place tubes in a vertical, balanced order into the cycler, e.g., two strips can be placed in the first and last column. foodproof® GMO Soya Identification 3 LyoKit KIT230108 / 09 Kit for 96 reactions Store kit at 2 to 8 °C For food testing purposes FOR IN VITRO USE ONLY Made in Germany Hygiena® | Camarillo, CA 93012 USA | diagnostics.support@hygiena.com | www.hygiena.com