ROCKER Tanfil 100 Tangential Flow Filtration System Instruction Manual

Instruction Manual for ROCKER models including: Tanfil 100 Tangential Flow Filtration System, Tanfil 100, Tangential Flow Filtration System, Flow Filtration System, Filtration System, System

A5 Tanfil 100, TFF System Instruction Manual RO-OPE184-EN202401-A06

TinaHuang

INSTRUCTION MANUAL

Citato da 3 — AC Socket and Fuse Holder (Input). 6. Reservoir. 12. AC Socket for Magnetic Stirrer (Output). (2). Operation Panel. Position. Designation. 1. Display. 2. MODE ...

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A5 Tanfil-100-TFF-System Instruction-Manual RO-OPE184-EN202401-A06

INSTRUCTION MANUAL
Tangential Flow Filtration System
Model No.
Tanfil 100
Please read this instruction manual before using this product.

Table of Contents

1. Introduction

2. Important Notice

3. Unpacking

4. Product Description

(1) Tanfil 100, TFF System

(2) Operation Panel

(3) Parts Material List

5. Button / Symbol / Display Explication

(1) Buttons

(2) Symbols

(3) Display

6. Installation

(1) Main Part

1.1 Support Rod

1.2 Gauge Mounting Set

1.3 Magnetic Stirrer and Reservoir

1.4 Luer Fittings and Tubing

(2) Capsules (or Cartridges)

(3) Typical Set up of TFF System

7. Operation

(1) Important Notice Before Starting

(2) Tubing Break-in Procedure

(3) Cleaning and Flushing

(4) Sample Concentration, Purification, or Diafiltration

4.1 Diafiltration

(5) Draining

5.1 Drain by Gravity

5.2 Drain by Pump

8. Maintenance

9. Troubleshooting

1. Introduction
Tanfil 100 is a laboratory scale tangential flow filtration (TFF) system designed for performing concentration, purification and diafiltration of biomolecules, including proteins, peptides, and nucleic acids. This user-friendly system incorporates a peristaltic pump, 500 mL liquid reservoir, 2 pressure gauges, and magnetic stirrer, creating a simple and ready to use system. Its integrated design and versatile capability to accommodate various types of capsules and cartridges make Tanfil 100 an ideal choice for a range of applications, including evaluation, validation studies, and process development within the laboratory setting. * A capsule or cartridge with specific molecular weight cutoffs (MWCO) is required to perform TFF process. The relevant operating limits of used capsules and cartridges, please refer to its instruction manual. Applications
TFF system serves as an effective solution for preventing membrane fouling and separating targe molecules based on size differences. The efficiency makes it a preferred method for concentration, purification, and diafiltration of biomolecules. The applications include: - Concentration and purification of proteins, peptides, or nucleic acids. - Recovery of proteins, antibodies, extracellular vesicles (EVs) from cell culture media. - Isolation and concentration of extracellular vesicles (EVs), exosome, microvesicles (MVs). - Bacteria recovery or removal from solutions. - Cell debris removal from fermentation broth or cell culture media. - Buffer exchange or desalting before analysis.
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2. Important Notice
This instrument is designed for laboratory usage only. Please read this manual carefully before installing and operating. The instrument shall not be modified in any way. Any modification will void the warranty and may result in potential hazard. We are not responsible for any injury or damage caused by any non-intended purposes and modifying the instrument without authorization.
1. Check the voltage specified on the name plate and ensure it matches the line voltage in your location.
2. Install the instrument in a clean, dust-less, non-hazardous, and ventilated area under 50°C. 3. Never use the instrument with any corrosive, flammable, combustible, or solvent liquids. 4. Overpressure or thick liquids can trigger the motor's self-protection, leading to automatic
shutdown. Please switch it off for at least 5 minutes before restarting. 5. Before each start, please check for kinks or restrictions in the tubing. Pressure buildup may
cause tubing and hoses to burst or rupture. 6. Pump tubing may rupture due to continuous contact with moving parts; it is advisable to
replace it according to the tubing manufacture's replacement interval. 7. Never move the system while it is operating, or when capsule and cartridge is connected. 8. In case of any issues with instrument, please contact the manufacturer or your service agent
for assistance. Do NOT disassemble it improperly. 9. Please discard packing materials in accordance with relevant local regulations. 10. Visit the official website and refer to the latest product guide for more information. 11. Operating Condition
(a) Ambient temperature: 5~ 50°C (b) Relative humidity: 80% RH Max. (c) Power supply: 100-240V~, 50/60Hz, 50W (d) Fuse: T1.5A, 250V (e) Altitude: up to 2000 m (f ) Pollution degree: II (g) Indoor use
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Do NOT start the system with the pump head open. Pressure in tubing should be less than 40 psi, or the tubing would be expanded due to overpressure. Please turn the instrument off and disconnect it from the main power before replacing the fuse. * Before operation, please check whether the compatibility of contact materials of instrument with sample solution.
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3. Unpacking

Please check if the package is complete without any damage before unpacking. When unpacking, please make sure you have all accessories that indicated on the list. If there is any problem, please keep the serial number along with packing case and contact your local distributor immediately for assistance.

3 10

5 Model
Tanfil 100

Standard Package Includes:

Tanfil 100, TFF System (Main Unit)

Support Rod

Feed Gauge Mounting Set

Retentate Gauge Mounting Set

Capsule Bracket Set

Reservoir (Including Lid Kit)

Magnetic Stirrer

Fittings / Parts Bag

Power Cord

Instruction Manual

The following items are packed in Fittings / Parts Bag:

Three-Way Stopcock *4

Screw Clamp for Tubing *1

2 m Tube-ULTR-C *1 (translucent)

Male Luer Coupler *2

Female Luer Lock - 1/8" hose barb *2

0.5 m Tube-Pharmed BPT *1 (opaque)

Male Luer Rotating Lock - 1/8" hose barb *9

Male Luer Rotating Lock - 1/8" elbow hose barb *2

Spring Plate *2

Rod of Spring Plate *2

Male Luer Lock - 1/8" hose barb *4

Tubing Clamps *14

Disc Filter *1

Hex Wrench *1

4. Product Description
(1). Tanfil 100, TFF System

Position 1 2 3 4 5 6

Designation Main Unit
Operation Panel Pump Head
Magnetic Stirrer Feed Gauge Mounting Set
Reservoir

Position

Designation

Retentate Gauge Mounting Set

Capsule Bracket Set

Capsule / Cartridge (not included)

Power Switch

AC Socket and Fuse Holder (Input)

12 AC Socket for Magnetic Stirrer (Output)

(2). Operation Panel

Position 1 2 3 4

Designation Display
MODE Button Up / Down Button Start / Stop Button

(3). Parts Material List

Part

Material

Part

Material

Reservoir

PES

Gauge Wetted Parts

SS316

Reservoir Lid

Gauge Mounting Block

Reservoir O-ring

Silicon

Luer Fittings

SS316, PP

Magnetic Stir Bar

PTFE Coated

Luer Fitting O-ring

Silicone

Three-Way Stopcock

PC, POM

Tubing - ULTR-C

Platinum-Cured Silicone

Tubing - Pharmed BPT

TPE*

* TPE: polypropylene-based thermoplastic elastomer (TPE)

5. Button / Symbol / Display Explication

(1). Buttons

Buttons

MODE START STOP

Designation Up Button Down Button MODE Button START / STOP Button

Description
Change setting value. (Hold the button to change value continuously)
Change setting value. (Hold the button to change value continuously) Switch setting parameters, such as rotational speed (rpm), time, pump head direction.
Start or stop the operation. (Hold the button for cleaning mode at Max. rpm)

(2). Symbols

Symbols

Designation

/ Type of Timer

Description Counter / Countdown Timer

Pump Head Direction CW / CCW

Fast Clean Mode

(3). Display 1

Run at Maximum speed (600 rpm) with pump head direction (CW / CCW)

Running Mode

Position 1 2 3

Designation Rotational Speed (rpm) Pump Head Direction
Timer (sec, min)

5
Fast Clean Mode

Position 4 5

Designation Type of Timer Fast Clean Mode

(1). Main Part 1.1 Support Rod

6. Installation

(A) Insert the support rod on the specified hole, ensuring the flat side of the rod faces the back side of main unit.
(B) Use provided hex wrench (2.5 mm) to loosen the screw located in the back plate of main unit. (see above diagram)
(C) Once the support rod touches bottom, securely fasten by screwing it back in.
1.2 Gauge Mounting Set

Feed

Retentate

Installed Feed and Retentate Gauge Sets Nut Removal of Feed Gauge Mounting Set
(A) Remove all male luer plugs from both the feed and retentate gauge mounting sets and install the three-way stopcock and male luer rotating lock - 1/8" hose barb as shown above. Install the three-way stopcock on the front of the retentate set if necessary.
(B) Remove the nut beneath the thumb screw of feed gauge mounting set.
It's easier to remove the nut by fixing it and rotating the thumb screw.
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Retentate Gauge Mounting Set
Feed Gauge Mounting Set

Capsule Bracket Set

(C) Ensure the gauge faces forward, then install the feed gauge mounting set by aligning the pin with the hole on the case and securing it by fastening the thumb screw.
(D) Install the capsule bracket set through the support rod first and secure it by fastening the thumb screw.
(E) Install the retentate gauge mounting set through the support rod with gauge facing forward, and secure it on the upper side of the support rod by fastening the thumb screw.

1.3 Magnetic Stirrer and Reservoir

AC Socket for Magnetic Stirrer

Reservoir Magnetic Stirrer

Installed Rods and Spring Plates

(A) Position the magnetic stirrer into the left compartment of the main unit, and plug its adaptor into the AC socket at the back of the main unit labeled "Max. 5W".
(B) Set the reservoir on the magnetic stirrer with outlet toward left side of the main unit.
To optimize stir bar performance, ensure the reservoir is centered on the magnetic stirrer.
(C) Screw the 2 rods of spring plate into threaded holes at rear of the left compartment, then press spring plate through the rods until the reservoir is securely fixed.
1.4 Luer Fittings and Tubing

Male Luer Plugs

Three-Way Stopcock

(A) Remove a male luer plug from outlet of the reservoir and install a three-way stopcock onto it.
(B) Remove a male luer plug from lid of the reservoir.
General operation can be performed without the lid kit by placing the concentrate tube directly into the reservoir. If performing diafiltration, place the lid onto reservoir and connect 2 fittings simultaneously.
See p.19 (4). 4.1 Diafiltration. 11

Rententate Tube Male

Male

Pump Tube

Tubing Clamp

Male

Concentrate Tube Male (elbow)

Male Male

Male: Male Luer Rotating Lock - 1/8" hose barb Male (elbow): Male Luer Rotating Lock - 1/8" elbow hose barb
Install a tubing clamp over each tubing connected to the hose barb. Tighten clamp by
pinching it.
(C) Cut 2 pieces of ULTR-C tubing (translucent) as retentate and concentrate tubes, and install fittings as shown above.
If performing without lid kit, cut concentrate tube to appropriate length to reach the reservoir and do not install the male (elbow) fitting on the reservoir side.
(D) Use Pharmed BPT tubing (opaque) as pump tube and install fittings as shown above. (E) Connect Pharmed BPT tubing (opaque) to three-way stopcocks at reservoir outlet and
feed gauge mounting set. (F) Insert pump tube into open pump head and close pump head onto tubing.
Pump tube can be clipped into the white clamp on the main unit to avoid shaking during
operation.
(G) Connect all tubing and capsule as show below. (H) If draining the residual liquid in system, cut ULTR-C tubing (translucent) to appropriate
length and connect it to one of the three-way stopcocks with a hose barb. See p.20 (5) Draining.

ULTR-C tubing

Pump Tube (Pharmed BPT tubing)
System Without Capsule

Concentrate Tube (ULTR-C tubing)

Retentate Tube (ULTR-C tubing)

Pump Tube (Pharmed BPT tubing)

System With Capsule 13

(2). Capsules (or Cartridges)
Example of connecting capsule and cartridge Left: Pall MinimateTM Capsule, Right: HansaBioMed TFF Cartridge (A) Remove the caps from both the feed and retentate ports of capsule.
Please keep all caps for storage of capsule or cartridge.
(B) Connect the feed port of capsule to the side of feed gauge mounting set using the provided luer fittings (and tubing).
(C) Connect the retentate port of capsule to the side of retentate gauge mounting set using the provided luer fittings (and tubing).
During operation, ensure all tubing is as short as possible to minimize the residual volume in the system. A screw clamp can be clamped on concentrate tube to adjust the pressure and filtrate flow.
(D) Remove one of the filtrate caps of the capsule and attach a male or female luer lock 1/8" hose barb on it.
(E) Attach a piece of ULTR-C silicone tubing to filtrate hose barb and place the other end in the filtrate collecting vessel.
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(F) Adjust capsule bracket set to appropriate position to hold the capsule. The rings on bracket can be adjusted by loosening screw with a hex wrench (2.5mm).
(3). Typical Set up of TFF System
Pressure Gauges Capsule / Cartridge

Reservoir
Sample (Concentrate)
Peristaltic Pump

Filtrate
Operation Panel

7. Operation

(1). Important Notice Before Starting There are several procedures during operation, please follow the instruction for system set up. The recommended operational details and parameters may vary based on the specific capsules and cartridges; therefore, refer to the manufacture's instructions for proper handling.
Confirm all fittings and tubing are securely connected without any leaks before running liquid. When using new tubing, work with a break-in period to prevent tubing splits and ensure stable
flow rates, refer to p.17 (2) Tubing Break-in Procedure. Load the tubing onto the center of pump head's rotor to prevent inappropriate wear or
premature rupture. Open the pump head when not in operation to prevent the tubing from remaining in a
deformed state and optimize its performance lifetime. While operating with the lid kit sealed on reservoir, please open a port and/or attach a disc
filter to it.

The general procedure is outlined below, but the actual process may vary depending on whether a capsule or cartridge is being used.

1
System Cleaning

2 Capsule
Cleaning and Testing

3
Sample Processing

4
Sample
Recovery

5 System &
Capsule Cleaning

Refer to

Step 1

p.18 (3) Cleaning and Flushing

(4). Sample Concentration, p.19 Purification, or Diafiltration

p.20 (5). Draining

Step 2 V
V

Step 3 V

Step 4 V

Step 5 V
V

(2). Tubing Break-in Procedure Performing the tubing break-in procedure is highly recommend while using new Pharmed BPT tubing (opaque) or peristaltic tubing. This process enhances tubing flexibility and elasticity, preventing damage and sample loss. Please ensure to break the tubing in before running the sample.
(A) Install the Tanfil 100 main unit and tubing as instructed in Chapter 6. Installation, without attaching a capsule or cartridge. The system setup for break-in procedure is shown above.
(B) Pump the system and recirculate with water at maximum rpm for 15 to 20 minutes. (C) After the break-in period, drain the system by running water into waste vessel. (D) Open the pump head and examine the tubing for completeness without damage. (E) Reload the tubing, close the pump head, and it's ready for next procedure.
Ensure the tubing is loaded onto the center of pump head's rotor to prevent inappropriate
wear or premature rupture.
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(3). Cleaning and Flushing Cleaning the system without attaching the capsule is recommend for the first use or when idle for an extended period. Follow the setup outlined in this section for all the cleaning and flushing procedures.
For cleaning the system, connect both gauge mounting sets with ULTR-C tubing (translucent). For cleaning the capsule or cartridge, connect it to the system as instructed on p.14 Capsules
(or Cartridges). Refer to the manufacture's instructions of the cleaning solution for the capsule. If the cleaning solution becomes dirty, please promptly replace it with a new buffer and repeat
the cleaning cycle to maintain optimal cleaning effectiveness.

System Cleaning

Capsule Cleaning

(A) Install the Tanfil 100 main unit and tubing as instructed in Chapter 6. Installation, and adjust the three-way stopcocks as shown above.
(B) Fill the reservoir with 250 mL of purified and filtered water and recirculate for 15 minutes. 0.1 N Sodium Hydroxide (NaOH) at 35~45 °C can be used to sanitize the system.
The cleaning solution at 35~45 °C can enhance the cleaning effectiveness.
(C) Adjust the three-way stopcocks as instructed on p. 20 (5) Draining, then pump the cleaning solution to waste. If the capsule or cartridge does not allow air to run into it, discount them before the draining
step or until the solution level drops to the bottom of reservoir.
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(4). Sample Concentration, Purification, or Diafiltration Repeat the setup and instructions on p.18 (3) Cleaning and Flushing, but substitute the sample liquid for purified water or cleaning solution. A screw clamp can be clamped on concentrate tube to adjust the pressure and filtrate flow. Refer to the manufacture's instructions for operating parameters of capsules, such as pressure.
4.1 Diafiltration The lid kit of reservoir is necessary for continuous diafiltration. The common application of diafiltration includes desalting and buffer exchange.
(A) Place and seal the lid kit onto the reservoir, and remove the male luer plug from lid. (B) Attach the hose barb fittings (male luer rotating lock - 1/8" elbow hose barb * 2) to lid. (C) Connect a ULTR-C tubing (translucent) from the hose barbs on lid to the hose barb on
the retentate gauge mounting set. (D) Connect a ULTR-C tubing (translucent) to another hose barb on lid and place another
end of tubing in a vessel filled with a buffer solution. During diafiltration, please close the left port on the lid to ensure a complete seal. (E) Adjust the direction of three-way stopcocks as instructed on p.18 (3) Cleaning and Flushing and start the processing.
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(5). Draining Draining is used to remove the liquid from the system, such as cleaning solution or sample liquid. Therefore, it always follows the cleaning, concentration, or diafiltration procedure. Two different setups for draining are available: by gravity or by pump. Follow the setup outlined in this section for the desired method.
If the capsule or cartridge does not allow air to run into it, discount them before the draining step or until the solution level drops to the bottom of reservoir.

Drain by Gravity

Drain by Pump

5.1 Drain by Gravity (A) Attach a hose barb fitting (Male Luer Lock - 1/8" hose barb) to the three-way stopcocks
at the outlet of the reservoir. (B) Connect a ULTR-C tubing (translucent) to hose barb and place another end of tubing in
a vessel. (C) Adjust the three-way stopcock at outlet of the reservoir toward the direction of hose
barb. The solution should now drain by gravity. While operating with the lid kit sealed on reservoir, please open a port and/or attach a disc
filter to it.

5.2 Drain by Pump (A) Attach a hose barb fitting (Male Luer Lock - 1/8" hose barb) to the three-way stopcocks
at the front of feed gauge mounting set. (B) Connect a ULTR-C tubing (translucent) to hose barb and put the other end of tubing in a
vessel. (C) Adjust the three-way stopcock at outlet of the reservoir toward direction of hose barb. (D) Start pumping to eliminate the solution.
While operating with the lid kit sealed on reservoir, please open a port and/or attach a disc
filter to it.
8. Maintenance
1. Please operate the instrument in a well-ventilated area and keep it clean. Ensure to unplug it before cleaning.
2. The instrument is not autoclavable. Please clean the surface by pure water or 75% ethanol. 3. Open the pump head when not in operation to prevent the tubing from remaining in a
deformed state and optimize its performance lifetime. 4. If there's any solution drop or splash to instrument, please switch off and unplug it
immediately and clean the surface to prevent damage from penetration or corrosion. 5. If fuse blows, please troubleshoot and solve problems first. When replace fuse, get the spare
fuse from the fuse holder by a flathead screwdriver. 6. O-ring(s), tubing, and disc filter are consumables, it is recommended to replace it on a yearly
basis or as needed to ensure good operation. As for tubing, follow tubing manufacture's recommended replacement interval.
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9. Troubleshooting

Problem

Reason and Solution

Abnormal Display

Loose plug Reconnect plug to power supply. Burnt fuse Replace with a new fuse. Display or components failure Contact distributor for assistance.

Motor Shutdown

Pump module overload Slow down speed, release screw clamp, then restart the instrument.

No Stir with Magnetic
Stirrer

Loose plug Reconnect plug to power supply on back plate. Disorder of magnetic stirrer Contact distributor for assistance.

Expansion of Silicone Tube

Overpressure of silicone tube Slow down speed, release screw
clamp, then replace with a new silicone tube. Capsule block Replace with a new capsule.

184100-01(02) 203100-01(02) 203100-10 184100-40 184100-62 184100-47 184100-48

10. Ordering Information
Tanfil 100, Tangential Flow Filtration System, AC100-240V, 50/60Hz, US plug (EU plug) MS 100, Low Profile Magnetic Stirrer, AC100-240V, 50/60Hz, US plug (EU plug) PTFE Magnetic Stirring Bar, Ø 3.6 x 25 mm Reservoir Set Tanfil 100 Repair Kit TYGON®, Sani-Tech® Ultra-C Platinum-cured Silicone Tubing, ID 1/8" x OD 1/4", 50 ft Pharmed® BPT Tubing, ID 1/8" x OD 1/4", 25 ft

Pall, MinimateTM TFF Capsule

HansaBioMed, TFF Hollow Fibers

· Optional Capsules / Cartridges

PALOA001C12

Pall, 1K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA003C12

Pall, 3K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA005C12

Pall, 5K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA010C12

Pall, 10K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA030C12

Pall, 30K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA050C12

Pall, 50K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA070C12

Pall, 70K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA100C12

Pall, 100K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA300C12

Pall, 300K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA500C12

Pall, 500K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

PALOA990C12

Pall, 1000K MinimateTM TFF Capsule with OMEGA membrane (1/pkg)

HBM-TFF1

HansaBioMed, TFF-Easy Polysulfone hollow fibers, 5 nm (1/pkg)

HBM-TFFMV

HansaBioMed, TFF-MV Polysulfone hollow fibers, 200 nm (1/pkg)

HBM-TFFEVS-S

HansaBioMed, TFF-EVs Polyethersulfone hollow fibers, 50 ± 10 nm (1/pkg)

RO-OPE184-EN202401-A06

Rocker Scientific Co., Ltd.
Tel: +886-2-26033311 Fax: +886-2-26036622 E-mail: export@rocker.com.tw https://www.rocker.com.tw

References

rocker.com.tw

Acrobat Distiller 19.0 (Windows)