ACCURIS INSTRUMENTS Smart QTM 100 Fluorometer Instruction Manual

Instruction Manual for ACCURIS INSTRUMENTS models including: Smart QTM 100 Fluorometer, Smart QTM 100, Fluorometer

Zaid Saud

Operation Manual

File Info : application/pdf, 26 Pages, 1.22MB

F1001-User-Manual-V1 01

Operation Manual
V1.1
This Product is for Research Use Only

Thank you for purchasing the Smart-QTM 100 Fluorometer. This manual details the instrument's features, specifications, as well as complete operating instructions; please read it carefully prior to operation. Keep this user manual for later use. Important: Please keep the box and packaging material for this instrument. If service is required, the box will be needed to ship the instrument to our Service Department.
Initial Inspection Please inspect the instrument as well as all included accessories when you first open the packaging. If you find anything damaged or missing, please contact Benchmark Scientific or your local distributor immediately.
BENCHMARK SCIENTIFIC / ACCURIS INSTRUMENTS PO Box 709 Edison, NJ 08818 USA Phone: 908-769-5555 Website: www.benchmarkscientific.com / www.accuris-usa.com Email: info@benchmarkscientific.com / info@accuris-usa.com

Safety Warning and Guidelines
1. Important information for safe use Users should have a clear understanding of how to use this instrument before operation, please read this manual carefully prior to operation.
NOTICE: Please review this manual in its entirety before operating the Smart-QTM 100.
For Research Use Only. This instrument is not a medical device and is not intended to be used for clinical testing.
2. Operation and Maintenance The operation and maintenance of the instrument should comply with the basic guidelines and warnings below. Incorrect operation or maintenance will have detrimental effects on the life, performance, and safety features of the instrument.
This instrument is for indoor use only and conforms to I class B type common equipment of standard GB9706.1.
CAUTION: Biological Contamination All samples used with this instrument, including quality control samples, calibration samples, and components should be considered infectious and treated with caution. Always wear gloves and appropriate safety gear for protection. The instrument should not be disassembled by the end user unless under the guidance of Benchmark's Service Department.
The instrument should only be installed in an environment with standard room temperature, low dust, low humidity, and away from direct sunlight, electromagnetic interference, and heat sources.
Always power off the instrument when you are finished using it. Unplug the power cord and cover the instrument with a cloth or plastic sheet to prevent excessive dust from entering the housing.

Unplug the instrument immediately if any of the following occur, and contact Benchmark Scientific's Service Department: Liquid enters the Instrument. The instrument is dropped or the outer shell damaged. The functions of the instrument have obviously changed.
3. Maintenance The instrument housing should be cleaned regularly using a soft, damp cloth. To clean the touchscreen, disconnect the power cable, and clean the touchscreen with a soft cloth lightly moistened with LCD cleansing detergent. Take care not to use excessive force as this can damage the touchscreen. Do not use abrasive cleaning solutions or material to prevent the touchscreen from getting scratched. To disinfect the instrument, disconnect the power cable and use a soft cloth lightly moistened with 70% ethanol, 70% isopropanol, or 10% bleach (0.6% sodium hypochlorite). Take care so that the cleaning solution does not enter the power inlet, the sample port, or the USB drive ports.
4. Transportation and Storage Requirements Ambient temperature: 10 ~ 40 Relative humidity: 70% Place in a well-ventilated room, away from corrosive gas.

Table of Contents
Chapter 1 Introduction ............................................................................................................1 Chapter 2 Specifications.........................................................................................................2
1. Required Installation Environment ..............................................................................2 2. Specifications...............................................................................................................2 Chapter 3 Instrument Overview .............................................................................................3 Chapter 4 Installation ..............................................................................................................4 4.1 Initial Check .........................................................................................................4 4.2 Installation Conditions..........................................................................................4 4.3 Operating Conditions ...........................................................................................4 Chapter 5 Software Introduction............................................................................................6 5.1 Instrument Startup ...............................................................................................6 5.2 Main Menu Interface ............................................................................................6 5.3 dsDNA Interface...................................................................................................7 5.3.1 dsDNA Interface...................................................................................................8 5.3.2 Calibration Curve Interface ..................................................................................9 5.3.3 Sample Measurements........................................................................................9 5.3.4 Molarity Calculator .............................................................................................10 5.3.5 Normalization Calculator....................................................................................11 5.4 Fluorescence Interface ......................................................................................12 5.5 Working Solution Calculation Settings...............................................................13 5.6 Standard Curve Interface...................................................................................14 5.6.1 New Standard Curve .........................................................................................14 5.6.2 Curve Management ...........................................................................................16 5.7 Reports Interface ...............................................................................................16 5.8 User Management Interface ..............................................................................17 5.9 Settings Interface ...............................................................................................18

5.9.1 System Settings Interface..................................................................................18 5.9.2 Upgrade Interface ..............................................................................................19 5.9.3 Maintenance Interface .......................................................................................19 Chapter 6 Troubleshooting...................................................................................................20

Smart-Q 100 Fluorometer Operation Manual Introduction
Chapter 1 Introduction

Chapter 1

The Smart-QTM 100 Fluorometer is used for the precise quantitation of nucleic acids and proteins in microliter volumes via fluorescent detection. Under conditions of low concentrations of molecules, concentration values are linear to fluorescence intensity. Thus, unknown samples can be quantitatively analyzed. Reagent kits with fluorescent dye, buffer, and standards are available from Accuris or your local distributor.

Key Features and Benefits: 7-inch color LCD touch screen display, easy to operate Fast and accurate detection of DNA, RNA and proteins (< 6 sec) Low sample consumption, only 1-20L of sample is needed for accurate quantification Data storage for up to 10,000 sample records Sample data can be printed or exported via USB drive Customized sample standard curve Reagent calculator to assist with volume determinations
Auto sleep function for power-saving

Smart-Q 100 Fluorometer Operation Manual

Chapter 2 Specifications

1. Required Installation Environment
Environmental Temperature: 10°C40°C Relative Humidity: 70% Input Voltage: 24 VDC 2A

2. Specifications

Model Display Dynamic Range
Light Source
Excitation Wavelength
Emission Wavelength
Stability Detector Measurement Speed Calibration type Consumable type Input Voltage Dimensions (WxDxH) Weight

Smart-QTM 100 Fluorometer 7.0-inch, color touch screen 5 orders of magnitude Blue LED Red LED Blue: 460 ± 20nm Red: 625 ± 20nm Green: 525 570nm Red: 670 725nm 1.5% Photodiodes (300-1000nm) 5 seconds 23-point standard 0.5mL optically clear tube 24 VDC 2A 16.1 x 28.7 x 6.1cm / 6.34 x 11.30 x 2.40in. 1.6kg / 3.5lbs

Smart-Q 100 Fluorometer Operation Manual

Structure

Chapter 3 Instrument

Chapter 3 Instrument Overview

This chapter provides an overview of the physical characteristics of the Smart-Q 100. When using the instrument for the first time, please read this chapter carefully to familiarize yourself with all the parts.
Front/Side:

Lid

Back:

Fig 3.1.01 Front/Side View

Sample holder
Display Screen
PC port LAN port USB port

Fig 3.1.02 Rear View 3

Power input port

Smart-Q 100 Fluorometer Operation Manual Installation
Chapter 4 Installation

Chapter 4

4.1 Initial Check
Each Smart-Q 100 has been factory-inspected prior to shipping. Please refer to the included packing list to ensure that all items are included. Contact Accuris or your local distributor if there are any signs of damage to the packaging upon receipt.

Carefully check the instrument's appearance and housing for cracks, damage, or deformation.
4.2 Installation Conditions
Install the instrument on a clean, dry, and level surface in a well-ventilated room away from corrosive agents

Installation Steps 1. Carefully remove the instrument from its packaging and place it onto a stable worktable. Remove all plastic and tape. 2. Unpack the 24V 2A power adapter and plug it into the socket on the back of the instrument. Then connect another side to an AC100~240V power outlet. 3. Turn on the power. The instrument will start to self-check after powering on.
4.3 Operating Conditions
The Smart-Q 100 uses 0.5ml fluorescent PCR tube to test samples. Sample test steps: Insert the 0.5mL tube with sample or standards into sample holder. Close the lid; Click the measurement button on the interface to perform a measurement.

Smart-Q 100 Fluorometer Operation Manual Installation

Chapter 4
Fluorescent PCR tube

Fig 4.3.01 Instrument Operation Diagram

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Chapter 5 Software Introduction

5.1 Instrument Startup
Power the instrument on. Upon start-up, the instrument will perform a self-check (Fig. 5.1.01). After the self-check, the Login Interface will be displayed (Fig. 5.1.02).

Fig 5.1.01 Self-check

Fig 5.1.02 Login Interface

5.2 Main Menu Interface

After entering the user-specific password (Admin password is set by default to "0000"), the Main Menu Interface will be displayed (Fig. 5.2.01).

Fig 5.2.01 Main Menu Interface 6

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Name

Table 5-1 Main Menu Interface Operations Function
Select to manage account settings or to switch between users.
Select to view the working solution calculator

Select to enter the dsDNA Interface

Select to enter the RNA Interface

Select to enter the Oligo Interface

Select to enter the Protein Interface

Select to enter the Fluorescence Interface

Select to enter the Standard Curve Interface

Select to view a list of the most recent protocols created/run

Select to enter the settings interface

5.3 dsDNA Interface
Note: As the interface of dsDNA, RNA, Protein, Oligo are identical, Only the dsDNA Interface is described in detail in this manual.

From the Main Menu Interface, click

to enter the dsDNA Interface. Three test

types are available for selection: dsDNA: High Sensitivity, dsDNA: Broad Range, & 1x dsDNA (Fig. 5.3.01).

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Fig 5.3.01 dsDNA test type

Fig 5.3.02 dsDNA test

5.3.1 dsDNA Interface
Select the test type and click "OK" to enter next interface. The following takes dsDNA: High Sensitivity as an example. The specific functions are as follows:
: Select an available standard curve. : Input the sample name.
: Select the appropriate units: ng/L, ng/mL, g/L, g/mL, mg/mL.
: Click to enter the Calibration Curve Interface (see Chapter 5.3.2).
: Click to print all current protocol test data.

: Click to perform a sample measurement.
: Click to modify the protocol name.
: Set the volume of the original sample, range 1~20L. Original Conc.: Calculated concentration of the original sample. RFU (Relative Fluorescence Units): Calculated fluorescence value of the original sample.
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Chapter 5 Software

5.3.2 Calibration Curve Interface
Prior to sample measurements, a calibration curve must be created using the standards that are typically included with a quantification kit. Only 2 standards are required to form the calibration curve. Enter the dsDNA testing interface. Click the button and select "Calibration" to create the calibration curve (Fig. 5.3.03).

Fig 5.3.03 Curve Cal.

Fig 5.3.04 First Cal. point

Fig 5.3.05 Second Cal. point

For each assay, you have the choice to run new standards, or to use values from the

previous calibration to calibrate the assay. If a calibration has been carried out recently,

the last set data can be selected; If recalibration is required, select the

button

to perform the calibrate.

Enter the calibration interface, enter the known concentration value for each standard, and press "Read Standards" to perform measurements of the standards. After both standards have been read, select "Save" to save the calibration data.
5.3.3 Sample Measurements
After generating the calibration curve, input the original volume of the sample that was added to the tube and click to perform a measurement. After measurement, the original concentration and RFU of the sample will be displayed. Swipe right to view data from multiple samples within the same run. Click the name of the sample to modify the sample's name.

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Fig 5.3.06 Testing interface

Fig 5.3.07 Test Report

: Delete selected test results.

: Export all result of current testing.

: Print all result of current testing.

Fig 5.3.08 Modify sample name

: Click to enter the Molarity/Normalization Calculator Interface.

: Click to enter the options interface. The reagent kit number and sample ID can be adjusted in this interface.

5.3.4 Molarity Calculator

Click

and select the "Molarity" tab to enter the Molarity interface.

Fig 5.3.09 Molarity/Normalization Calculator Interface 10

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Select the conversion units: ng/L, ng/mL, g/L, g/mL, mg/mL. After selecting the conversion units, select the units: M, mM, M, nM, pM.

Input the molecular weight; the default value for dsDNA is set to 660g/mol.

Input the DNA length, when "Auto-populate DNA length" in enabled, all lengths will be automatically filled in according to the length of the first sample. Note: The Molarity/Normalization Calculator Interface can only be selected one sample measurements have been performed.
5.3.5 Normalization Calculator
Select the Normalization tab to enter the Normalization calculator interface (Fig. 5.3.10). In this interface, choose the type of Normalization (for example: molarity, concentration, and/or weight), and set the final sample molarity and volume. Click "Calculate" to enter the results interface (Fig. 5.3.11).

Fig 5.3.10 Normalization Interface

Fig 5.3.11 Results Interface

The Filling List tab shows the required sample and buffer volume to be added to achieve the desired final sample concentration within the final sample volume. On the Conc. Ratio interface, if a secondary dilution is required, the dilution ratio and the

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

concentration after dilution will be displayed (Fig. 5.3.12). The Initial Conc. interface shows the concentration of the original sample (Fig. 5.3.13). Click "Export" to export current data to an inserted USB drive.

Fig 5.3.12 Secondary Dilution

Fig 5.3.13 Initial Concentration of Sample

5.4 Fluorescence Interface
From the Main Menu Interface, select to enter the Fluorescence interface. Three test types are available for selection: Blue 470nm, Red 635nm, & Blue & Red (Fig. 5.4.01).
Note: The Fluorescence interface does not require the generation of a calibration curve.

Fig 5.4.01 Fluorescence Test Type

Fig 5.4.02 Blue 470nm Channel 12

Fig 5.4.03 Blue & Red Channels

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Test Types: Blue 470nm and Red 635nm are single excitation wavelength tests, whereas Blue & Red is a dual-wavelength test (Fig 5.4.03). The Blue 470nm interface is identical to the Red 635nm interface. Click to begin a sample measurement. After measurement, the RFU of the sample will be displayed (Fig. 5.4.02). Data can be deleted, exported, or saved via the icons found at the upper right corner of the display.

5.5 Working Solution Calculation Settings

Click and drag down the

button to view the Working Solution Calculator (Fig.

5.5.01). This interface allows for the calculation of the total working solution volume

required based on dye-to-buffer ratios. Input the number of samples and standards

required and select "Done". The calculated volumes for "Dye", "Buffer", & "Total

volume" will be displayed based on the set numbers of samples and standards. Select

"Include Overage" to account for 1 extra tube in the calculation.

Detection Range Calculator: From the Working Solution Calculator interface, swipe right to enter the Detection Range Calculator (Fig. 5.5.02). Here, the assay type, units, and original sample volume can be adjusted, and a detection range will be displayed after clicking "Done".

Fig 5.5.01 Working Solution Calculator Fig 5.5.02 Detection Range Calculator 13

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

5.6 Standard Curve Interface
From the Main Menu Interface, click 5.6.01).

to enter the Standard Curve Interface (Fig.

Fig 5.6.01 Standard Curve Interface
: Select the sample type (dsDNA, RNA, Oligo, Protein). : Create a new standard curve. : Delete, import, or export the standard curve.
: Sort the list in alphabetical order. : Sort the list by type of assay.
: Sort the list by creator name.
5.6.1 New Standard Curve
Click the icon to create a new standard curve. The New Curve Selection Box will be displayed, with adjustable values for sample type, assay type, and curve name (Fig. 5.6.02). Once the values have been adjusted/set, click "OK" to enter the New Standard Curve Interface (Fig. 5.6.03).
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Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Fig 5.6.02 New Curve Selection Box

Fig 5.6.03 New Standard Curve Interface

: Highlight the previous standard.

: Highlight the next standard, or to add an additional standard.

: View Parameter Settings (EX / EM wavelengths + concentration units).

: Plot the obtained sample data points to a curve.

: Delete RFU1/RFU2/RFU3 data or to delete the standard itself.

: Perform standard sample measurement.

Note: At least 5 standards are required to create the standard curve. For each standard, the average RFU value taken from 3 readings is used for curve generation.

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

5.6.2 Curve Management

From the Standard Curve Interface Click to enter Curve Management Interface

(Fig. 5.6.04).

Fig 5.6.04 Curve Management Interface

Fig 5.6.05 Importing Curve(s)

: Delete the selected curve.

: Export the selected curve(s) to a USB drive (Fig 5.6.04).

: Import curve(s) from a USB drive to the instrument (Fig. 5.6.05).

5.7 Reports Interface
In the main interface, click to enter the Reports Interface (Fig. 5.7.01). Input keywords in the search box to quickly find the required report (Fig 5.7.02).

Fig 5.7.01 Reports Interface 16

Fig 5.7.02 Quick Search

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Click on a report to view its saved data (Fig. 5.7.03). Click on the sample from the row to view the detailed sample report (Fig. 5.7.04).

Fig 5.7.03 Saved Report Data
5.8 User Management Interface

Fig 5.7.04 Detailed Sample Report

From the Main Menu Interface, select the icon from the upper left corner of the display. The following icons will be displayed:
: User Settings: Administrators can manage user accounts by adding, deleting, or modifying the user name / password. Non-Administrators can only change their own passwords in this interface.
: Switch User: Return to the Login Interface to switch to a different user.

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

5.9 Settings Interface

Click

in the main interface to enter the Settings Interface (Fig. 5.9.01).

System Settings Upgrade Maintenance

Fig 5.9.01 Settings Interface

5.9.1 System Settings Interface

Fig 5.9.02 System Settings

Click

to enter system settings (Fig. 5.9.02):

Language: Select English, or Chinese.

Smart-Q 100 Fluorometer Operation Manual

Introduction

Chapter 5 Software

Date and Time: Set the system date and time. Screen Brightness: Adjust the screen brightness. Sleep Mode: Turn the automatic sleep mode on or off and adjust time required to initiate Sleep Mode.

5.9.2 Upgrade Interface

From the Main Menu Interface, click

to enter the Upgrade Interface (Fig 5.9.03).

The versions of the software and firmware will be displayed. To make updates, insert a

USB drive with the relevant update files and select the ">" next to either the software

or firmware version number.

Fig 5.9.03 Upgrade interface
5.9.3 Maintenance Interface

From the Main Menu Interface, click

and input the password to enter the

Maintenance Interface. This is only available for authorized service procedures.

Contact Accuris Instruments or your local distributor for details.

Chapter 6 Troubleshooting

No.

Issue

Analysis

Solutions

No display after turning on power

Power not reaching the instrument

Check the power supply; Check whether the plug is loose.

Check the power; Check whether the

module is loose, if loose

Light source not visible

Light source failure; Module poor contact Contact Accuris

Instrument or your local

distributor

Measurement not PCR tube isn't in

stable

place

Check the PCR tube and press it in place

Measurement not accurate

Standard curve is not accurate; Instrument detection is drifting

Check standard solution and reset standard curve; Calibrate standard curve

Quantification Kits and Detection Tubes

NS1020-HS-100 NS1020-BR-100 NS1020-T

High Sensitivity DNA Quantification Kit, 100 assays Broad Range dsDNA Quantification Kit, 100 assays 0.5mL Fluorescence Detection Tubes, pack of 250

References

GPL Ghostscript 9.55.0