Brochure for Thermo Fisher Scientific models including: Invitrogen, Thermo Fisher Scientific, DNA purification, DNA isolation, plasmid DNA, genomic DNA, viral DNA, plant DNA, DNA clean-up, nucleic acid quantitation, PureLink, MagMAX, GeneJET, Qubit, KingFisher, PCR, sequencing, genotyping, molecular biology, research, DNAzol Reagent, PureLink HiPure, PureLink Pro, MagMAX DNA, GeneJET, Qubit Fluorometer, KingFisher Flex, KingFisher Duo, KingFisher Presto, K210002, K210003, K310001, K310096A, K1820-00, A27828, Q32866

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DNA purification and analysis
Maximize sample yield, purity, and integrity

Optimized for maximum yield and purity

From plasmid to genomic DNA Purification technology overview

3

and from DNA clean-up to

Plasmid DNA purification kits

4

automation, InvitrogenTM products

bring flexible, innovative

Genomic DNA purification kits

10

solutions to meet virtually every researcher's needs.

Genomic DNA purification kits--

tissue and cell samples

12

Explore the InvitrogenTM

Genomic DNA purification kits--

blood and serum samples

14

portfolio of leading solutions,

kits, and benchtop devices

Plant genomic DNA

isolation kits

16

for reliable results.

Viral genomic DNA

purification kits

18

DNA clean-up solutions

19

Complete purification systems for

nucleic acids, proteins, and cells

22

Nucleic acid quantitation

24

Ordering information

26

Tools for success in your molecular biology research
2

Purification technology overview
A variety of purification technologies and chemistries to meet your purity needs

We bring a large portfolio of purification kits to suit all your nucleic acid purification needs. From plasmid DNA to genomic DNA, you'll find what you're looking for. InvitrogenTM nucleic acid purification products are optimized to provide maximum yield, purity, and integrity from virtually any sample type and application. We offer a wide range of

specialized nucleic acid purification products based on three highly developed purification technologies: silica membrane, anion-exchange resin, and switchable surface charge. Let us help you determine which plasmid or genomic DNA (gDNA) purification kit is appropriate for your needs.

Genomic DNA product selection guide

Overview

Low throughput or manual

Medium throughput and spin-column technology

High throughput and 96-well High throughput and

filter plate

magnetic-bead technology

Type

Organic

Spin column

Spin column

Magnetic beads

Product name DNAzolTM Reagent

PureLinkTM kits

PureLinkTM Pro gDNA kits

MagMAXTM DNA kits

Tissue Cells

DNAzol Reagent

PureLink gDNA Mini

MagMAX DNA Multi-Sample

Blood Plant

DNAzol BD Reagent Plant DNAzol Reagent

PureLink gDNA Plant

PureLink Pro 96 gDNA

MagMAX DNA Multi-Sample Ultra MagMAX Plant DNA Isolation

Buccal swabs Bacteria

Not recommended DNAzol Reagent

PureLink gDNA Mini

MagMAX DNA Multi-Sample Ultra

Viral

Not recommended

PureLink Viral RNA/DNA Mini PureLink Pro 96 Viral RNA/DNA MagMAX Viral Nucleic Acid

Scalable or automatable

No

No

Yes

Yes

Compatible with

the KingFisher No

No

No

Yes

system

qPCR

Yes

Yes

Yes

Yes

NGS

Yes

Yes

Yes

Yes

Plasmid DNA product selection guide

Overview

Low throughput or manual

Low-to-medium throughput and high purity

Product name GeneJETTM kit

PureLinkTM HiPure

PureLinkTM Expi

and HiPure Filter kits Endotoxin-Free Kit

Purity grade Molecular

Transfection

Advanced transfection

High throughput and high purity

PureLinkTM Pro Quick96 kit

PureLinkTM 96 HQ kit

Molecular

Transfection

3

Plasmid DNA purification kits
Plasmid DNA that's pure and ready to go

If, during plasmid preparation, your DNA has low recovery, contains impurities, or just doesn't perform in your downstream experiments, you can choose from our wide range of high-performing plasmid purification products. We offer a portfolio of plasmid purification products that support your downstream applications from cloning to transfection of sensitive cell lines. Table 1 shows a variety of formats of the InvitrogenTM PureLinkTM purification product lines, offering high yields with extremely low endotoxin levels for maximized downstream results. Kits that include these technologies are designed to isolate plasmid DNA at the purity and scale you need.

Which plasmid DNA isolation technology is right for you?

Purity grade

Molecular

Transfection

Kits
Endotoxin level
Yield up to Downstream application

GeneJET
Standard (1­10 EU/µg)
20 µg­1 mg Not sensitive · PCR · Cloning (digestion, ligation) · Sequencing · Nucleic acid labeling

PureLink HiPure
Low endotoxin (0.1­1 EU/µg)
20 µg­15 mg Sensitive · Standard transfections · All molecular biology
applications · In vitro transcription

Technology

Silica membrane

Total protocol time 15­60 min

Prep size

Mini­maxi

Anion exchange (resin) 30­120 min Mini­giga

Advanced transfection
PureLink Expi Endotoxin-Free
Endotoxin-free (<0.1 EU/µg)
1.5­15 mg Very sensitive · Primary and stem cell
transfection · Gene therapy and vaccine
(in vivo) research · Microinjection · All molecular biology
applications Anion exchange (membrane)
90­120 min
Maxi, mega, and giga

4

Plasmid DNA

PureLink Expi Endotoxin-Free Maxi, Mega, and
Giga Kits
Medium throughput

PureLink Pro Quick 96 Mini Kit

GeneJET Miniprep

Advanced transfection

Molecular grade (cloning/
sequencing)

High throughput (96-well format)

Downstream application Start here

Transfection

Low throughput Spin/vacuum column (silica)

PureLink Quick Mini Kit

GeneJET Midiprep and Maxiprep

Gravity-flow column (anion exchange)

PureLink HiPure Mini,
Midi, and Maxi Kits

PureLink HiPure Filter Maxiprep and Midiprep Kits

Low throughput
Vacuum-assisted (anion exchange)
PureLink HiPure Expi Megaprep and Gigaprep Kits

High throughput (96-well format)
PureLink 96 HQ Mini Plasmid DNA
Purification Kit

Table 1. Plasmid DNA purification products, prep sizes, and approximate yields.

Prep size

Overnight bacterial culture volume

Approximate yield

Miniprep

1­5 mL

Up to 40 µg

Midiprep

10­50 mL

Up to 300 µg

Maxiprep

100­200 mL

Up to 1 mg

Megaprep

500 mL­2.5 L

Up to 5 mg

Gigaprep

2.5­5 L

Up to 15 mg

For our complete portfolio of plasmid DNA purification products, go to thermofisher.com/plasmidprep
5

Plasmid DNA purification kits
Fast, easy to use, and cost-effective

The PureLink plasmid purification products have been developed to provide the greatest value for your money. With performance meeting or exceeding competitive offerings (Figure 1), and fair pricing, PureLink technology is the clear choice for plasmid purification in research laboratories.
InvitrogenTM PureLinkTM HiPure Expi low-endotoxin plasmid purification kits Fast, easy-to-use protocols, and an anion-exchange resin allow you to purify plasmid DNA at a quality equivalent to that obtained from purifying plasmid DNA by passing it through a cesium chloride gradient twice--one of the most rigorous methods for plasmid purification. In less than 2 hours, plasmid is pure enough for transfections with no need for additional steps to remove contaminants like RNA, proteins, and endotoxins. Additionally, the use of phenol, chloroform, ethidium bromide, and cesium chloride are eliminated, minimizing exposure to and disposal of hazardous materials.

High yield Isolate up to 5 mg (megaprep) and up to 15 mg (gigaprep) of high-quality plasmid DNA from a single purification using 0.5­5 L of bacterial culture.
Purity Achieve inherently low endotoxin levels (typically 0.1­1.0 EU/g), and A260/A280 >1.8, making it ideal for mammalian cell transfection.
Simplicity and speed With the vacuum-assisted protocol, plasmid isolation typically takes only 90 minutes to complete (Figure 2).

Yield (mg) HiPure Expi QIAfilter

7 6 5 4 3 2 1 0
0.25 L 0.5 L 1 L HiPure Expi kit

0.25 L 0.5 L 1 L QIAfilter plasmid kit

Figure 1. InvitrogenTM PureLinkTM HiPure Expi kits, with advanced anion exchange technology, produce superior yields of transfection-quality DNA. Comparison of the total yields of InvitrogenTM pcDNATM 3.3 plasmid purified using PureLink HiPure Expi technology versus megapreps from another supplier, with increasing bacterial culture volumes.

Giga Mega Giga Mega

Resuspension + lysis Neutralization Clarification Purification Precipitation

0 50 100 150 200 250 300
Protocol time (min)
Figure 2. PureLink HiPure Expi kits offer superior yields of ultrapure, transfection-quality plasmid DNA with an accelerated protocol time. The combination of advanced anion exchange technology and vacuum assistance used with PureLink HiPure Expi kits dramatically reduces overall protocol time.

Comparisons on this page based on internal data.
6

7

Plasmid DNA purification kits
High throughput, reliable, and reproducible

Our mini-scale portfolio of high-throughput plasmid purification products for molecular- and transfectiongrade applications provides high-quality plasmid DNA. Technologies include silica membrane columns and 96-well

plates, anion exchange columns, and magnetic beads. With the PureLink Pro Quick96 Plasmid Kit, the resulting plasmid DNA is >90% supercoiled, with no detectable genomic DNA or RNA (Figure 3).

Which high-throughput PureLink plasmid purification kit is right for you?

MagJETTM Plasmid DNA Kit

PureLinkTM 96 HQ

PureLinkTM HiPure

PureLink Pro Quick96 Mini Plasmid DNA

Plasmid Miniprep Kit Plasmid Kit

Purification Kit

Protocol time Variable

<90 minutes

45 minutes

Variable

Purity grade

Molecular

Transfection

Molecular

Transfection

Yield of plasmid DNA
High throughput­ compatible
Technology

Up to 25 µg Yes Magnetic beads

Up to 40 µg No

Up to 15 µg Yes

Up to 60 µg Yes

Anion-exchange resin Silica-based spin/ vacuum plates

Silica-based spin/ vacuum plates

Product size

96 preps, 4 x 96 preps 25 preps, 100 preps 4 x 96 preps

4 x 96 preps

Figure 3. High purity of plasmid DNA isolated using the PureLink Pro Quick96 Plasmid Kit. InvitrogenTM pcDNATM 3.1/His/lacZ plasmid DNA (5 g), purified using a liquid-handling robot and the PureLink Pro Quick96 Plasmid Kit, shows high yields of supercoiled plasmid DNA with no detectable genomic DNA or RNA contamination.
8

9

Plasmid DNA

Genomic DNA purification kits

Maximize process efficiency and downstream performance
We offer a range of InvitrogenTM genomic DNA purification kits for sensitive, scalable purification from an expansive set of starting materials to maximize process efficiency and downstream performance. This includes a broad range of kits for purifying genomic DNA from a wide variety of samples, including tissues, cells, blood, serum, plants, and forensic samples.

PureLink Pro 96 Genomic DNA Purification Kit

MagMAX DNA Multi-Sample Ultra Kit

Plate

Magnetic beads

PureLink Microbiome DNA Purification Kit

Spin

PureLink Pro 96 Genomic DNA Purification Kit

MagMAX Plant DNA Isolation Kit

Magnetic beads

Plate High throughput­compatible
Low throughput

Silica spin column

Organic extraction

MagMAX FFPE DNA/RNA Ultra Kit

RecoverAll Total Nucleic Acid
Isolation Kit for FFPE

High throughput­compatible Low throughput

PureLink Genomic Plant DNA
Purification Kit

Plant DNAzol Reagent

Magnetic beads

High throughput­compatible (plate format)
Silica

MagMAX DNA Multi-Sample Ultra Kit
Find the complete portfolio of genomic DNA purification products at thermofisher.com/gdnaprep
10

PureLink Pro 96 Genomic DNA Mini Kit

Genomic DNA

MagMAX-96 DNA Multi-Sample Ultra Kit
and MagMAX Cell-Free DNA

PureLink Pro 96 Genomic DNA Purification Kit

Magnetic beads

Silica

High throughput­compatible (plate format)

DNAzol BD Reagent

PureLink Genomic DNA Mini Kit

Organic extraction

Silica spin column

Low throughput

Bacteria

Blood or serum

Magnetic beads

Buccal

Column

MagMAX DNA Multi-Sample Ultra Kit
PureLink Genomic DNA Mini Kit

FFPE

Sample type
(Start here) Virus

Plant

Tissues and cells

Plate
High throughput­compatible (plate format)

PureLink Pro 96 Genomic DNA Purification Kit
PureLink Pro 96 Viral RNA/DNA
Purification Kit

Low throughput (silica spin column)

Magnetic beads

MagMAX Pathogen RNA/DNA Kit

Low throughput
Magnetic beads Multi-sample

Organic extraction

Silica spin column

PureLink Viral RNA/DNA Mini Kit

MagMAX DNA Multi-Sample Ultra Kit

DNAzol Reagent

PureLink Genomic DNA Mini Kit

11

Genomic DNA purification kits-- tissue and cell samples
High-yield genomic DNA from tissues and cells
We offer several different kits for purifying genomic DNA from a variety of tissue and cell sample types. We offer a range of genomic DNA extraction kits for sensitive, scalable purification from an expansive set of starting materials to maximize process efficiency and downstream performance. This includes a broad range of kits for purifying genomic DNA from a variety of samples, including tissue and cells, to meet your research needs. The InvitrogenTM PureLinkTM Genomic DNA Mini Kit enables high-yield, high-purity genomic DNA (gDNA) extractions from a wide variety of sample types (Figure 4). Use of this kit enables genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

% Eluted in E1 (200 µL)

35

5 x 105 cells

91.1%

30

5 x 106 cells

% in E1
25

26.5
20

15

10
4.5
5

0 PureLink Genomic DNA Mini Kit

90

80

71.0%

70

60

50

40

15.0

30

20
3.4
10

0 Supplier Q kit

Figure 4. Higher, more concentrated yields achieved with the PureLink Genomic DNA Mini Kit compared to that obtained with another supplier's kit. E1 is referring to the first elution.

Total yield (µg)

12

Genomic DNA

Which genomic DNA purification kit is right for your tissue or cell samples?

DNAzol Reagent

PureLink Genomic DNA Mini

PureLink Pro 96 Genomic DNA

MagMAXTM-96 DNA Multi-Sample

Process the largest amount of tissue

Fast isolation of gDNA

from a variety of

High-yield, high-purity Rapid and automated

samples

gDNA in a plate format extraction of DNA

Tissue starting material

Up to 50 mg

Up to 25 mg

Up to 25 mg

Up to 25­50 mg, depending on tissue type

Yield

Up to 250 µg from tissue 5­10 µg from tissue

5­10 µg from tissue

10­80 µg from tissue

Isolation method

Organic extraction

High

No

throughput­

compatible

Compatible Cloning, qPCR, applications sequencing

Silica spin column No

Silica filter plate Yes

Scalable, flexible format with magnetic beads
Yes

Cloning, qPCR,

Cloning, qPCR,

Cloning, qPCR,

sequencing, genotyping sequencing, genotyping sequencing, genotyping

Prep time 10­30 min

15 min

35 min

45 min

Prep size 100

50, 250

4 x 96

50, 96, 500, 2,500

13

Genomic DNA purification kits-- blood and serum samples
Rapid and efficient extraction of genomic DNA from human blood and serum
The emergence of pharmacogenomic centers of excellence has resulted in increasing needs for purification of high-quality genomic DNA from a large of volume of blood. Since samples are collected and shipped worldwide, every sample may differ with regard to storage and shipping conditions. Choosing the right genomic DNA purification kit is important based on your needs and expected end results. We offer a wide range of kits designed to isolate genomic DNA from your blood or serum samples at the purity and scale you need. Figure 5 shows gel image of gDNA purified from whole blood samples using the InvitrogenTM GeneCatcherTM gDNA Blood Kit.
L I G I G I G I G I G I G I GL
Figure 5. Purification of gDNA from archived whole blood samples using the GeneCatcher gDNA Blood Kit. An 0.8% agarose gel was used to evaluate gDNA extractions from archived whole blood samples (blood samples frozen for >8 years). Blood samples were split into 2 equal aliquots, and gDNA was extracted using the GeneCatcher gDNA Blood Kit and a kit from another supplier G. The GeneCatcher kit extractions employ magnetic beads, while supplier G's kit uses a traditional lysis and DNA precipitation method that has been shown to co-purify agents that are inhibitory to downstream applications. I = GeneCatcher extractions; G = supplier G extractions; L = InvitrogenTM 1 Kb DNA Extension Ladder, largest band 40 kb.
14

Genomic DNA

Which genomic DNA purification kit is right for your blood or serum samples?

DNAzolTM BD

PureLink Genomic DNA Mini

PureLink Pro 96 Genomic DNA

MagMAX-96 DNA Multi-Sample

Process the largest Fast isolation of gDNA High-yield, high-purity Rapid and automated

amount of blood from blood

gDNA in a plate format extraction of DNA

Blood input

500 µL

200 µL

200 µL

50 µL­1 mL

Yield

10­20 µg

3­10 µg

3­10 µg

1.5­66 µg

Isolation method Organic extraction

High throughput­ No compatible

Compatible applications

Cloning, qPCR, sequencing

Silica spin column No

Silica filter plate Yes

Scalable, flexible format with magnetic beads
Yes

Cloning, qPCR,

Cloning, qPCR,

Cloning, qPCR,

sequencing, genotyping sequencing, genotyping sequencing, genotyping

Prep time

10­30 min

15 min

35 min

45 min

Prep size

100

50, 250

4 x 96

50, 96, 500, 2,500

15

Plant genomic DNA isolation kits
Successful plant DNA extraction that's easy on you

Plant tissue is difficult enough to work with due to the high levels of polysaccharides and polyphenols present. Therefore, compounding the complexity with tedious, inefficient methods will only prolong the process of isolation. Cetyl trimethyl ammonium bromide (CTAB) methods require excessive time and handling, limiting your throughput. Some silica membrane­ and magnetic bead­based protocols don't remove inhibitors inherent in plant samples that can carry over into the final product and interfere with downstream applications. This leads to

frequent sample processing failure, necessitating that you repeat the purification--if you have the time and sample to spare. Now we offer you products specifically designed for easy, high-yield, and high-purity DNA purification from plant samples. Inhibitors are removed for reliable downstream results. Make your DNA isolation from plant samples easier on you and easier on your samples, while achieving high-yield, high-purity results with InvitrogenTM and Applied BiosystemsTM plant molecular biology reagents.

16

Genomic DNA

Which genomic DNA purification kit is right for your plant samples?

Plant DNAzolTM Reagent

PureLinkTM Genomic DNA Plant Mini

PureLinkTM Pro 96 Genomic DNA

MagMAXTM Plant DNA

High-quality gDNA Most cost effective at a great value

Fast isolation of gDNA

from a variety of

Rapid and automated

plant samples

extraction of DNA

Plant tissue input 100 mg

100 mg

Up to 25 mg

100 mg

Yield

Varies based on starting material

Isolation method Organic extraction

High throughput­ No compatible

Compatible applications

Cloning, qPCR, sequencing

1­15 µg Silica spin column No

5­10 µg from tissue Silica filter plate Yes

Varies based on starting material
Scalable, flexible format with magnetic beads
Yes

Cloning, qPCR,

Cloning, qPCR,

Cloning, qPCR,

sequencing, genotyping sequencing, genotyping sequencing, genotyping

Prep time

60 min

40 min

35 min

40 min

Prep size

~330

50

4 x 96

96

Price per prep $0.58

$3.60

$2.62

$2.12

Empower your plant science research: go to thermofisher.com/agbio to learn more and to purchase our products and solutions.
17

Viral genomic DNA purification kits
High-yield genomic DNA from tissue and cells
Purification of viral nucleic acids poses unique challenges for getting good recovery and detection sensitivity. Capture and lysis of virus particles from very dilute solutions or cell-free samples is the first hurdle. We've developed DNA purification products that are optimized to provide maximum viral DNA yield, purity, and integrity from a broad range of sample types in several format options. For example, the InvitrogenTM PureLInkTM Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments (Figure 6) that are high quality and show reliable performance in PCR, restriction enzyme digestion, cloning, and labeling.

Which genomic DNA purification kit is right for your viral samples?

PureLinkTM Viral RNA/DNA Mini

PureLinkTM Pro 96 Viral RNA/DNA

Fast isolation of viral nucleic acid

Easy to use with high sensitivity

Sample input

500 µL cell-free sample

200 µL cell-free sample

Compatible samples

Plasma, serum, cerebrospinal fluid

Plasma, serum, cerebrospinal fluid

Isolation method

Silica spin column

Filter plate

High throughput­

No

compatible

Compatible applications Cloning, qPCR, sequencing, genotyping

Prep time

15 min

Prep size

50

Yes
Cloning, qPCR, sequencing, genotyping 35 min 4 x 96

MagMAXTM Pathogen RNA/DNA Kit Rapid and automated extraction of viral nucleic acid 300 µL cell-free sample Plasma, serum, saliva, blood
Scalable, flexible format with magnetic beads Yes
Cloning, qPCR, sequencing, genotyping 45 min
480

For more information on Invitrogen viral RNA/DNA purification and detection products, go to thermofisher.com/viral
18

DNA clean-up solutions for every downstream application

Whether isolating a specific size of DNA from complex PCR mixtures or recovering bands from agarose gels, we have solutions that will meet your needs. Kit formats offer simple and rapid PCR clean-up using spin columns or magnetic beads; 96-well plates, with flexible size selection; and one-tube, 5-minute protocols. Isolated DNA is ready for sequencing, PCR, transcription, cloning, and labeling.

PureLink Quick Gel Extraction Kit

Gel slice

Gel slice or PCR

Starting material
(Start here)

PureLink Quick Gel Extraction and PCR Purification Combo Kit

Spin column
PureLink PCR Purification Kit and
PCR Micro Kits

PCR reaction
Magnetic bead
ChargeSwitch PCR Clean-Up Kit

96-well filter silica plate
PureLink Pro 96 PCR Purification Kit

DNA clean-up

19

Solubilize gel

Spin

Bind

Aqueous wash

Vacuum

Figure 6. PureLink Quick Gel Extraction Kit protocol. Gel fragments are first solubilized to release the DNA. The sample is then loaded on a PureLink spin column and isolated using a simple "bind, wash, and elute" procedure. DNA fragments are recovered in TE buffer or water in a ready-to-use format.

20

Spin
Vacuum

Elute Spin

Puri ed DNA

Which DNA clean-up kit is right for you?

Product
PureLinkTM PCR Purification Kit
PureLinkTM Pro 96 PCR Purification Kit
PureLinkTM PCR Micro Kit
PureLink Quick Gel Extraction Kit
PureLinkTM Quick Gel Extraction Kit and PCR Purification Combo Kit

Cat. No.
K310001 K310002
K310096A
K210010 K310050 K310250
K210012 K210025
K220001

Quantity
50 preps 250 preps
4 plates (4 x 96 rxns)
10 preps 50 preps 250 preps
50 preps 250 preps
50 preps

Protocol time (min) <15 20 10
<30 10­30

DNA clean-up application PCR clean-up
PCR clean-up

Format

Elution volume (µL)

Silica spin/

50

vacuum column

96-well silica plate 50­150

PCR clean-up

Silica spin column 5­20

Gel extraction

Silica spin/ vacuum column

PCR clean-up

Silica spin/

and gel extraction vacuum column

30­100 30­100

DNA clean-up

21

Complete purification system for nucleic acids, proteins, and cells

Successful downstream analysis depends on high-quality, reproducible purification of nucleic acids, proteins, and cells. Thermo ScientificTM KingFisherTM purification systems are designed to deliver high-quality results with minimal hands-on time, automating a significant part of your workflow.
· Choose from five distinct systems to meet your application and throughput needs
· Optimized kits streamline the purification workflow for a wide variety of sample types
· Thermo ScientificTM BindItTM Software enables you to create customized protocols for additional flexibility
· Specially designed consumables allow efficient sample processing

Magnetic separation technology KingFisher systems use permanent magnetic rods and disposable tip combs to collect, transfer, and mix magnetic particles:
1. When the magnetic rod--sheathed inside the tip comb-- is lowered into the solution, magnetic beads collect at the bottom of the tip comb.
2. The tip comb is then positioned in a different row or plate, and the beads are released by moving the magnetic rods out of the tip comb.
3. The tip comb facilitates the mixing of reagents with the beads as the magnetic head moves up and down.

22

Invitrogen automated sample preparation technologies comparison chart

Thermo ScientificTM KingFisherTM Duo Prime Purification System

Thermo ScientificTM KingFisherTM FlexTM system

Thermo ScientificTM KingFisherTM PrestoTM Purification System

Benefits
Application
Reagents
Protocol Plastic consumables Sample input volume Throughput Instrument dimensions (W x D x H) Weight

An economical option for automated nucleic acid extraction and protein purification from up to 12 samples at a time and 24 samples per load using magnetic beads

A highly versatile and reproducible purification of 24 or 96 samples per run

Utilizes magnetic particle­based technology to provide high-quality yields of target nucleic acids and proteins in high-throughput laboratories

DNA and RNA isolation from various starting materials; proteomic applications; cell isolation

DNA and RNA isolation from various starting materials; proteomic applications; cell isolation

Nucleic acid purification, protein purification, immunoprecipitation, antibody purification, phosphopeptide enrichment, phage display

Preloaded and user-editable Applied BiosystemsTM MagMAXTM kits available to support tissue, cells, blood, FFPE, bacteria, buccal, plant, viral samples, and liquid (e.g., serum)

Preloaded and user-editable MagMAX kits available to support tissue, cells, blood, FFPE, bacteria, buccal, plant, viral samples, and liquid (e.g., serum)

Preloaded and user-editable MagMAX kits available to support tissue, cells, blood, FFPE, bacteria, buccal, plant, viral samples, and liquid (e.g., serum)

Using BindIt Software or USB memory device

Using BindIt Software

Using BindIt Software

96 deep-well plate 24 deep-well plate 1 x 12 elution strip

96 deep-well plate 24 deep-well plate 96-well plate

96 deep-well plate 24 deep-well plate 96-well plate

30­1,000 L (12-pin magnet head) 200­5,000 L (6-pin magnet head)

50­1,000 L, 96 deep-well plate 200­5,000 L, 24 deep-well plate 20­200 L, 96-well plate

50­5,000 L 24- or 96-head magnets

Up to 12 with 12-pin magnet head Up to 6 with 6-pin magnet head

96 or 24 samples

96 or 24 samples

400 x 460 x 340 mm (15.7 x 18.1 x 13.4 in.)

680 x 600 x 380 mm (26.8 x 23.6 x 15 in.)

36.0 x 46.5 x 40.0 cm (14.2 x 18.3 x 15.5 in.)

17 kg (37.5 lb)

28 kg (62 lb)

24 kg (53 lb)

23

Nucleic acid quantitation
Accurate, sensitive, and specific quantification of DNA and RNA

For nucleic acid quantitation, InvitrogenTM QubitTM technology employs extreme selectivity not possible with absorbance measurements, resulting in accuracy high enough to quantitate even the most dilute or low-abundance samples, while still leaving enough sample for downstream applications (Figure 7).
Benefits of using Qubit technology · Better selectivity and accuracy than absorbance assays
· Effectively quantitates dilute and low-abundance samples
· Available for DNA and RNA samples
Upon binding to nucleic acid, the fluorescence of the Qubit dyes increases several hundred-fold, giving a very high signal-to-noise ratio for exceedingly high sensitivity--up to 1,000 times more sensitive than absorbance readings.

The Qubit 2.0 Fluorometer utilizes specifically designed fluorometric technology using InvitrogenTM dyes that only fluoresce when bound to DNA, RNA, or protein. These fluorescent dyes emit signals only when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. This specificity allows you to get very accurate results because Qubit technology only reports the concentration of the molecule of interest, not contaminants. Qubit fluorometric quantitation provides exceptionally specific and sensitive DNA and RNA quantitation, even at low concentrations (Figure 8).
Qubit assay kits for the Qubit 2.0 Fluorometer contain reagent, premade calibration standards and premade buffer, and are also available in quantities of 500 assays. Please go to thermofisher.com/qubit for a full product list.

Qubit 2.0 Fluorometer for accurate, precise benchtop quantitation of DNA and RNA Use of the InvitrogenTM QubitTM 2.0 Fluorometer for nucleic acid quantitation is:
· Selective--each Qubit assay kit is highly selective for a single analyte (RNA, DNA, or protein)
· Sensitive--samples with concentrations as low as 10 pg/L of DNA and 12.5 g/mL of protein may be accurately and reliably quantified
· Simple and intuitive--the Qubit 2.0 Fluorometer provides the same high accuracy you've come to expect, but now is even faster and requires less effort to use

24

A

DNA + RNA (1:1)

DNA

0.0

1.0

2.0

DNA (ng)

B

DNA + RNA (1:1)

0

10

20

DNA (ng)

DNA

Fluorescence Fluorescence

RNA

RNA

0 20 40 60 80 100 DNA or RNA (ng)

0 200 400 600 800 1,000 DNA or RNA (ng)

Figure 7. InvitrogenTM QubitTM High-Sensitivity and Broad-Range DNA Assay Kits. (A) The Qubit High-Sensitivity DNA Assay Kit and (B) the Qubit Broad-Range DNA Assay Kit have linear detection ranges of 0.2­100 ng and 2­1,000 ng, respectively. Each kit is selective for dsDNA, even in the presence of an equal mass of RNA. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. In the 1:1 mixture, the total mass of nucleic acid at a given point is double what is stated on the x-axis.

Concentration measured
Concentration measured

Qubit dsDNA HS Assay

Qubit 2.0 Fluorometer

NanoDrop spectrophotometer

12

Accuracy: Qubit vs. NanoDrop--low end of the range 1.6

10

1.4

1.2

1.0

8

0.8

0.6

6

0.4

0.2

0

4

0.01

0.02

0.03

0.1

DNA (ng/µL)

2

0

0.01

0.02

0.03

0.1

0.5

1

2

4

DNA (ng/µL)

6

8

10

Figure 8. Accuracy and precision of the Qubit 2.0 Fluorometer. Ten replicates of lambda DNA at concentrations of 0.01­10 ng/L were assayed using the InvitrogenTM QubitTM dsDNA HS Assay on the Qubit 2.0 Fluorometer according to the standard kit protocol. The same concentrations of DNA were measured in ten replicates using a Thermo ScientificTM NanoDropTM ND-1000 Spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates. Error bars represent the standard deviations of the 10 replicates. The concentrations indicated are those of DNA in the starting samples, before dilution in the InvitrogenTM QubitTM assay tubes.

25

Ordering information Product Plasmid purification kits PureLink HiPure Plasmid Miniprep Kit
PureLink Quick Plasmid Miniprep Kit
PureLink Pro Quick 96 Plasmid Purification Kit PureLink HQ Mini Plasmid DNA Purification Kit PureLink HiPure Plasmid Midiprep Kit
PureLink HiPure Plasmid Filter Midiprep Kit PureLink HiPure Plasmid Maxiprep Kit
PureLink HiPure Plasmid Filter Maxiprep Kit
PureLink HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit
PureLink HiPure Expi Plasmid Megaprep Kit PureLink HiPure Expi Plasmid Gigaprep Kit PureLink Expi Endotoxin-Free Megaprep PureLink Expi Endotoxin-Free Megaprep Genomic DNA purification kits PureLink Genomic DNA Mini Kit
PureLink Pro 96 Genomic DNA Mini Kit PureLink Pro 96 Viral RNA/DNA Purification Kit PureLink Pro 96 Viral RNA/DNA Mini Kit PureLink Genomic Plant DNA Mini Kit DNAzol Reagent PureLink Microbiome DNA Purification Kit Plasmid clean-up and gel extraction kits PureLink Quick Gel Extraction and PCR Purification Combo Kit PureLink Quick Gel Extraction Kit
PureLink PCR Purification Kit
PureLink Pro 96 PCR Purification Kit
PureLink PCR Micro Kit

Quantity
25 preps 100 preps 50 preps 250 preps 4 x 96 rxns 4 x 96 preps 25 preps 50 preps 25 preps 10 preps 25 preps 10 preps 25 preps 10 preps 25 preps 4 preps 2 preps 4 preps 2 preps
10 preps 50 preps 250 preps 4 x 96 preps 4 plates (4 x 96 rxns) 50 preps 50 preps 100 mL 50 preps
50 preps 50 preps 250 preps 50 preps 250 preps 4 plates (4 x 96 rxns) 50 preps

Cat. No.
K210002 K210003 K210010 K210011 K211004A K210096 K210004 K210015 K210014 K210006 K210007 K210016 K210017 K210026 K210027 K210008XP K210009XP A31232 A31233
K1820-00 K1820-01 K1820-02 K182104A
133800-96A
12280-050 K183001 10503027 A29790
K220001 K210012 K210025 K310001 K310002 K310096A
K310050

26

Product Automated nucleic acid purification systems Instruments KingFisher Flex Purification System with 24 Deep-Well Head KingFisher Flex Purification System with 96 Deep-Well Head KingFisher Duo Prime Purification System Nucleic acid purification products MagMAX kits MagMAX-96 DNA Multi-Sample Kit MagMAX mirVana Total RNA Isolation Kit MagJET Plasmid DNA Kit MagMAX Cell-Free DNA Isolation Kit MagMAX Plant DNA Isolation Kit MagMAX mirVana Total RNA Isolation Kit MagMAX DNA Multi-Sample Ultra Kit MagMAX FFPE DNA/RNA Ultra Kit MagMAX Total Nucleic Acid Isolation Kit MagMAX Cell-Free DNA Isolation Kit MagMAX Pathogen RNA/DNA Kit Dynabeads mRNA DIRECT Purification Kit Nucleic acid quantitation Qubit Fluorometer Qubit Quantitation Starter Kit Qubit Quantitation Lab Starter Kit Qubit dsDNA BR Assay Kit
Qubit dsDNA HS Assay Kit
Qubit ssDNA HS Assay Kit Qubit Assay Tubes

Quantity

Cat. No.

1 each 1 each 1 each

5400640 5400630 5400110

96 preps 96 preps 96 preps 50 preps 96 Preps 96 preps 500 preps 1 kit 100 preps 1 kit 480 preps 5 mL

4413021 A27828 K2791 A29319 A32549 A27828 A25597 A31881 AM1840 A29319 4462359 61011

1 each
1 each
1 each 100 assays, 2­1,000 ng 500 assays, 2­1,000 ng 100 assays, 0.2­100 ng 500 assays, 0.2­100 ng 100 assays, 1­200 ng
Set of 500

Q32866
Q32871
Q32872 Q32850 Q32853 Q32851 Q32854 Q10212
Q32856

27

Find out more at thermofisher.com/dnaprep
For Research Use Only. Not for use in diagnostic procedures. © 2015­2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. DNAzol is a trademark of Molecular Research Center, Inc. NanoDrop is a trademark of NanoDrop Technologies LLC. COL03846 0817



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