TaqPath COVID-19, Flu A/B, RSV Combo Kit
Lab Readiness Checklist
A single test to detect and differentiate SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) A/B
Applied Biosystems™ TaqPath™ COVID-19, Flu A/B, RSV Combo Kit (Cat. No. A49867)
For use with:
- Applied Biosystems™ 7500 Fast Real-Time PCR Instrument
This document lists all instruments, consumables, and reagents recommended and required for installing the instrument, training, and running an experiment for the detection of RNA from SARS-CoV-2, Flu A/B, and RSV. Please review this list carefully to ensure all necessary materials are available before installation and training dates. Missing materials can delay your setup process. Intended uses of the products mentioned vary. For specific intended use statements, please refer to the product label.
Note: To avoid contamination, it is highly recommended that you have separate sets of pipettes for samples and extraction, PCR reaction setup, and any post-PCR processing. Ideally these steps should all be performed in separate laboratory spaces as well. Always follow necessary precautions when handling patient samples.
Ordering Information
TaqPath COVID-19, Flu A/B, RSV Combo Kit
| Product | Supplier | Storage | Cat. No. |
|---|---|---|---|
| TaqPath COVID-19, Flu A/B, RSV Combo Kit | Thermo Fisher Scientific | Details below | A49867 |
| Includes: TaqPath RT-PCR COVID-19, Flu A/B, RSV Kit • Multiplex assay targeting the SARS-CoV-2 S and N genes, influenza A/B matrix gene, RSV A/B, N gene/M gene, and bacteriophage MS2 • MS2 phage control TaqPath COVID-19, Flu A/B, RSV Control • COVID-19, Flu A/B, RSV Controls TaqPath Control Dilution Buffer TaqPath 1-Step Multiplex Master Mix, No ROX | Thermo Fisher Scientific | -30°C to -10°C Below -70°C -30°C to -10°C -30°C to -10°C |
Required materials not supplied
Real-time PCR
Ordering Information
Data analysis and reporting software
| Product | Supplier | Storage | Cat. No. |
|---|---|---|---|
| Pathogen Interpretive Software CE-IVD Edition v1.0.1 and SAE Administrator Console Dx Compatible with: • 7500 Fast Real-Time PCR Instrument (used with SDS Software v1.5.1 or 7500 Software v2.3) | Thermo Fisher Scientific | N/A | Contact us at 800-955-6288, option 3, option 1; or email at instrumentservices@thermofisher.com |
| Separate computer for the Pathogen Interpretive Software with at least 4 GB of RAM, at least 60 GB of available disk space, and one of the following operating systems: • Windows 10 (Professional), 64-bit • Windows 10 (Enterprise), 64-bit • Windows 7 (Professional), Service Pack 1, 64-bit | Various | N/A | Various |
Real-time PCR instrument
| Product | Supplier | Storage | Cat. No. |
|---|---|---|---|
| 7500 Fast Real-Time PCR Instrument (used with SDS Software v1.5.1 or 7500 Software v2.3) | Thermo Fisher Scientific | RT | 4351106 (with laptop computer) 4351107 (with desktop computer) |
Calibration plates (7500 Fast Real-Time PCR Instrument)
| Product | Supplier | Storage | Cat. No. |
|---|---|---|---|
| ABY Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well, 0.1 mL | Thermo Fisher Scientific | -20°C | A24734 |
| JUN Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well, 0.1 mL | Thermo Fisher Scientific | -20°C | A24735 |
| 7500 Fast Real-Time PCR Systems Spectral Calibration Kit I, 0.1 mL (contains background plate, region of interest (ROI) and calibration plates for FAM, JOE, NED, ROX, SYBR Green, TAMRA, and VIC dyes) | Thermo Fisher Scientific | -20°C | 4360788 (if recalibration is required) |
Nucleic acid extraction
Ordering Information
Kits and reagents
| Product | Supplier | Storage | Cat. No. |
|---|---|---|---|
| MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit | Thermo Fisher Scientific | RT | Recommended: A48383 |
| Ethanol, absolute (200 proof), ACS reagent-grade or equivalent | Fisher Scientific | RT | Recommended: BP2818100, BP2818500, BP28184 |
| Nuclease-Free Water (not DEPC-treated) or equivalent | Thermo Fisher Scientific or Fisher Scientific | RT | Recommended: AM9932 or BP2819-100, BP2819-1, BP2819-4, BP2819-10, BP2819-20 |
Automated processing tools
| Product | Supplier | Storage | Cat. No. |
|---|---|---|---|
| KingFisher Flex Purification System with 96 Deep-Well Head and Deep-Well Heating Block | Thermo Fisher Scientific | RT | 5400630 |
| KingFisher Flex 96 Deep-Well Heating Block (if currently using standard block)* | Thermo Fisher Scientific | RT | 24075430 |
| KingFisher 96 Tip Comb for Deep-Well Magnets | Thermo Fisher Scientific | RT | 97002534, A48438, A48414 |
| KingFisher Deep-Well 96 Plate | Thermo Fisher Scientific | RT | 95040450, A48305, A48424, 95040455 |
| 96-Well Microplate | Thermo Fisher Scientific | RT | 97002540, AB0796, AB1127, 136101 |
General laboratory equipment and consumables
Ordering Information
| Product | Supplier | Storage | Cat. No. |
|---|---|---|---|
| MicroAmp Fast Optical 96-Well Reaction Plate, 0.1 mL | Thermo Fisher Scientific | RT | 4346907 |
| MicroAmp Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL | Thermo Fisher Scientific | RT | 4346906, 4366932 |
| MicroAmp Optical Adhesive Film | Thermo Fisher Scientific | RT | 4360954, 4311971 |
| MicroAmp Adhesive Film Applicator | Thermo Fisher Scientific | RT | 4333183 |
| Sterile Aerosol Barrier (Filtered) Pipette Tips | Thermo Fisher Scientific | RT | thermofisher.com/pipettetips |
NOTE: To avoid contamination, it is highly recommended that you have separate sets of pipettes for samples and extraction, PCR reaction setup, and any post-PCR processing. Ideally these steps should all be performed in separate laboratory spaces as well. Always follow necessary precautions when handling patient samples.
General laboratory equipment and consumables, cont.
Ordering Information
| Product | Supplier | Recommended product | Cat. No. |
|---|---|---|---|
| 1-10 µL single channel adjustable volume pipette | Thermo Fisher Scientific | F1-ClipTip Variable Volume Single Channel Pipette (1-10 µL) | 4641320N |
| 10–100 µL single channel adjustable volume pipette | Thermo Fisher Scientific | F1-ClipTip Variable Volume Single Channel Pipette (10–100 µL) | 4641200N |
| 100–1,000 µL single channel adjustable volume pipette | Thermo Fisher Scientific | F1-ClipTip Variable Volume Single Channel Pipette (100–1,000 µL) | 4641230N |
| Multichannel pipette in 5–20 µL volume range | Thermo Fisher Scientific | E1-ClipTip Equalizer 384 8-Channel (2–125 µL) or E1-ClipTip Equalizer 384 12-Channel (2-125 µL) | 4672060BT or 4672070BT |
| Multichannel pipette in 50–1,000 µL volume range | Thermo Fisher Scientific | E1-ClipTip Equalizer 8-Channel Pipette (15–1,250 µL) (optional) | 4672100BT |
| 10–300 µL multichannel pipette | Thermo Fisher Scientific | E1-ClipTip Equalizer 8-Channel (10–300 µL) | 4672080BT |
| 30–850 µL multichannel adjustable volume pipette, 12-well (optional) | Thermo Fisher Scientific | E1-ClipTip 12-Channel Pipette (30–850 µL) (optional) | 4671090BT |
| 15–1,250 µL single or multichannel adjustable volume pipette (optional) | Thermo Fisher Scientific | E1-ClipTip Electronic 1-Channel Pipette (15–1,250 µL) (optional) or E1-ClipTip Electronic 8-Channel Pipette (15–1,250 µL) (optional) | 4670040BT or 4671100BT |
| Aerosol-resistant barrier pipette tips, various sizes (1–1,250 µL) | Thermo Fisher Scientific | Aerosol-resistant barrier tips compatible with F1-ClipTip and E1-ClipTip pipettes | Recommended: 94420153, 94420813 |
| Centrifuge with rotor for microplates and deep well plates | Thermo Fisher Scientific | Sorvall General Purpose Centrifuges, various | Various |
| Laboratory vortex with plate pad | Thermo Fisher Scientific | Various | Various |
| Biosafety cabinet | Thermo Fisher Scientific | Herasafe 2030i Class 2 A2 Biological Safety Cabinets or 1300 Series Class II, Type A2 Biological Safety Cabinet Packages | 51032334 or 1336 |
| PCR Workstation (optional) | Fisher Scientific | Combination PCR workstation | 36-101-8894 or 36-101-8897 |
| Benchtop microcentrifuge to accommodate 1.5 mL tubes | Thermo Fisher Scientific | mySPIN 6 Mini Centrifuge or Sorvall Legend Micro 21R Microcentrifuge, refrigerated | 75004061 or 75002447 |
| -20°C freezer | Thermo Fisher Scientific | TSX Series High-Performance -20°C Manual Defrost Freezers or similar | TSX2320FA |
| -80°C freezer | Thermo Fisher Scientific | TDE Series Ultra-Low Temperature Freezers or similar | TDE60086FA |
| 4°C refrigerator | Thermo Fisher Scientific | TSX Series High-Performance Laboratory Refrigerator or similar | TSX3005GA |
| Gloves | Fisher Scientific | Various | Various |
| Safety glasses | Fisher Scientific | Various | Various |
| Laboratory coat | Fisher Scientific | Various | Various |
| General purpose lab tissues/wipers | Fisher Scientific | Kimberly-Clark Professional Kimwipes Delicate Task Wipers | S47299 |
| Product | Supplier | Recommended product | Cat. No. |
|---|---|---|---|
| Microcentrifuge tube rack for 1.5 mL tubes | Thermo Fisher Scientific | Flipper Microtube Rack for 1.5 mL and 2.0 mL Tubes | 8760, 8770-11, 8780 |
| Cold blocks, or ice and ice buckets (to accomodate reagent tubes and RT-PCR plate) | Thermo Fisher Scientific | Benchtop coolers and PCR cooler | 5116-1300 and 05-403-00 |
| 1.5 mL RNase-free microcentrifuge tubes | Fisher Scientific or Thermo Fisher Scientific | Microcentrifuge tubes, nonstick, RNase-free, 1.5 mL (250 tubes) or Snap Cap Low Retention Microcentrifuge Tubes, 1.5 mL, graduated, sterile | Recommended: AM12450 or 21-402-903 |
| RNase-free conical tubes, 15 mL | Thermo Fisher Scientific | RNase-Free Conical Tubes, 15 mL (racked) (500 tubes) | Recommended: AM12500 |
| RNase-free conical tubes, 50 mL | Thermo Fisher Scientific | RNase-Free Conical Tubes, 50 mL (racked) (200 tubes) | Recommended: AM12501 |
| Nalgene bottles | Thermo Fisher Scientific | Nalgene Square PETG Media Bottles (125 mL) | 342020-0125 |
| Reagent reservoirs | Thermo Fisher Scientific | Matrix Reagent Reservoirs, polystyrene; 100 mL, individually wrapped, with divider | 95128085 |
| Serological pipettes | Thermo Fisher Scientific | Nunc Serological Pipettes, individually wrapped, paper/plastic peel packaging, plugged | 170357 or 170358 |
| Pipette filler | Fisher Scientific | S1 Pipet Filler | Various |
| 96-well plate holder | Thermo Fisher Scientific | MicroAmp Splash-Free 96-Well Base | 4312063 |
| Reagent dispenser (optional) | Thermo Fisher Scientific | Multidrop Combi Reagent Dispenser | Recommended: 5840300 |
Find out more at thermofisher.com/covid19flursv
For In Vitro Diagnostic Use. Intended use of the products mentioned vary. For specific intended use statements, please refer to the product label. © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Windows is a trademark of Microsoft Corporation. Kimwipes is a trademark of Kimberly-Clark Worldwide, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Cy is a trademark of GE Healthcare. COL113197 1220
