Innovative Diagnostic Solutions for Hygiene & Food Safety | Hygiena
User Guide for hygiena models including: MON89034, CBH351, Bt176, MON89034 GMO Maize Multiplex Detection Kit, GMO Maize Multiplex Detection Kit, Maize Multiplex Detection Kit, Multiplex Detection Kit, Detection Kit, Kit
GMO Maize Multiplex Detection Kit (MON89034, CBH351, Bt176) Ready Reference Guide
Identification des OGM | Hygiena
GMO Maize Multiplex Detection Kit (MON89034, CBH351, Bt176) | Hygiena
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DocumentDocumentfoodproof® SL GMO Maize Multiplex Detection Kit (MON89034, CBH351, Bt176) Ready Reference Guide Revision A, December 2023 Product No. KIT230218 PCR kit for the qualitative detection of MON89034, CBH351 and Bt176 DNA using real-time PCR instruments. Before starting, it is strongly recommended to read the entire product manual available on our website. PROGRAM SETUP Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels: u FAM (MON89034), VIC/HEX (CBH351), ROX (Bt176) and Cy5 (Internal Control). 95 °C 60 °C 50 °C 2 min | 10 min 1 cycle 15 sec | 60 sec* 40 cycles Pre-incubation: 1 cycle Step 1: 50 °C for 2 min Step 2: 95 °C for 10 min Amplification: 40 cycles Step 1 : 95 °C for 15 sec Step 2*: 60 °C for 60 sec * Fluorescence detection For some real-time PCR instruments the probe quencher as well as the usage of a passive reference dye has to be specified. This kit contains probes with a non-fluorescent "dark" quencher and no passive reference dye. DATA INTERPRETATION Verify results of positive (Control Template) and negative controls (H2O), before interpreting sample results. Always compare samples to positive and negative control. Review data from each channel and interpret results as described in the table. FAM + + + + - VIC/HEX + + + + - ROX + + + + - Cy5 + or + or + or + or + or + or + or - + - Result Interpretation Positive for MON89034, CBH351 and Bt176 Positive for CBH351 and Bt176 Positive for MON89034 and Bt176 Positive for MON89034 and CBH351 Positive for CBH351 Positive for MON89034 Positive for Bt176 Negative for MON89034, CBH351 and Bt176 Invalid foodproof® is a registered trademark of Hygiena. The above mentioned real-time PCR instruments are registered trademarks of their respective holders. RR-KIT230218-RevA PREPARATION OF THE PCR MIX Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves. Thaw reagents, mix (do not vortex!) and briefly spin vials before opening. +12.5 µL + 4 µL + 3.5 µL 1. PREPARE PCR MIX Add 12.5 µL Master Mix (2XM), 4.0 µL Primer/Probe Mix (P) and 3.5 µL PCR-grade H2O (not included) for each reaction to a suitable tube. (n samples + 2 controls + at least one additional reaction to cover pipetting loss). Mix carefully but thoroughly by pipetting up and down. +20 µL 2. ADD PCR MIX Pipette 20 µL of prepared PCR mix into each strip or plate well. +5 µL 3. ADD SAMPLES AND CONTROLS Pipette 5 µL of samples, negative control (PCR-grade H2O) or Control Template (C) into respective wells. 4. SEAL Carefully seal strips/plate. 5. CENTRIFUGE Briefly spin strips/plate in a suitable centrifuge. 6. START REAL-TIME PCR RUN Cycle samples as described above. foodproof® SL GMO Maize KIT230218 For food testing purposes Multiplex Detection Kit Kit for 50 reactions FOR IN VITRO USE ONLY (MON89034, CBH351, Bt176) Store kit at -15 to -25 °C Hygiena® | Camarillo, CA 93012 USA | diagnostics.support@hygiena.com | www.hygiena.com