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User Manual for heliXcyto models including: CCK-1-1 Dynamic Biosensors, CCK-1-1, Dynamic Biosensors, Biosensors
The heliXcyto Chip Cleaning Kit contains Cleaning Solution 1 and Cleaning solution 2 for manual cleaning of the heliXcyto chip outside of the heliXcyto device.
File Info : application/pdf, 5 Pages, 2.02MB
DocumentDocumentheliXcyto User Manual CHIP CLEANING KIT Detergent- and enzyme-based solutions for manual cleaning of chips Dynamic Biosensors GmbH & Inc. CCK-1-1 v1.1 Product Description Order Number: CCK-1-1 Table 1. Contents and Storage Information Material Cleaning Solution 1 (detergent-based) Lyophilized Enzyme Powder Tris-HCl Buffer (reconstitution buffer) Cap Transparent Red Transparent Amount 1 mL 5 mg 1 mL Storage RT 2-8°C 2-8°C The heliXcyto Chip Cleaning Kit contains Cleaning Solution 1 and Cleaning solution 2 for manual cleaning of the heli Xcyto chip outside of the heliXcyto device. For research use only. This product has a limited shelf life, please see expiry date on label. CCK-1-1 v1.1 www.dynamic-biosensors.com Page 1 of 4 Reconstitution The Cleaning solution 2 has to be reconstituted prior first use by dissolving the 5 mg lyophilized enzyme powder in 1 mL of Tris-HCl reconstitution buffer. Reconstituted Cleaning solution 2 should be aliquoted (10 100 µL volumes) and stored at -20 °C. Cleaning solution 1 is ready-to-use and does not require any preparation. CCK-1-1 v1.1 www.dynamic-biosensors.com Page 2 of 4 Application Note Manually cleaning heliXcyto chips extends their lifetime. This can be performed by injecting cleaning solutions into the microfluidic channel of the chip with a pipette. To reduce the introduction of dust particles into heliXcyto chips during manual handling and optimally wash out cell remnants it is recommended to: a. wear gloves when handling chips b. only use freshly filtered buffers c. always inject solutions into the entry port on the left side of the flipped chip heliXcyto chip cleaning protocol a. Fill the microfluidic channel with 10 µl fresh Cleaning solution 2 b. Seal chip in- and outlet with clear sticky tape (e.g. SellotapeTM, do not use tape that leaves glue remnants) c. Incubate at least 20 min at RT (or longer, e.g. overnight) d. Wash microfluidic channel 3 x with 10 µl Cleaning solution 1 e. Wash microfluidic channel 3x with 10 µl water (Milli-Q or similar) f. Empty chip by pipetting 10 µl air. CCK-1-1 v1.1 www.dynamic-biosensors.com Page 3 of 4 Contact Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 Woburn, MA 01801 USA Order Information order@dynamic-biosensors.com Technical Support support@dynamic-biosensors.com www.dynamic-biosensors.com Instruments and chips are engineered and manufactured in Germany. ©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved. CCK-1-1 v1.1 www.dynamic-biosensors.com Page 4 of 4