How to use NucleoAMP?

1st step

Transfer sample (e.g., stool, pre-enrichment liquid bacteria cell, swab, blood, saliva, body fluid, spice, food, plant and environmental samples) (20 µL for lyophilized kits and 200 µL for non-lyophilized kit) to a tube containing lysis fluid for the extraction of nucleic acid (DNA or RNA) in a single step.

2nd step

Incubate tubes for 15 minutes at 95 °C to perform nucleic acid extraction.

3rd step

Leave the tubes on cold block or ice and at room temperature for 5 minutes.

4th step

Transfer 20 µL (for lyophilized kit or 5 µL non-lyophilized kit) sample to individual reaction strips. Pipet up and down 5 times to mix.

5th Step

Transfer reaction strips in to novaAMP device.

6th Step

Select the desired program from the device to start. Positive results can be determined in 5-15 minutes but wait for 30 minutes for negative results (may show changes depending on kits and samples).

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