This operation manual is for the Model VB-M006 microscope. It is recommended to carefully review this manual prior to operating the device to optimize safety and performance. Keep this manual in an easily accessible place for future reference.
WARNING:
To prevent fire and electrical shock, do not expose the unit to rain or moisture.CAUTION:
No user-serviceable parts inside. Refer servicing to qualified service personnel.Contents
- 1. Before Use
- 2. Diagram
- 3. Assembly
- 4. Operation
- 5. Specification
- 6. Troubleshooting
- 7. How to install the monitor or camera
- 8. Microscope Camera (Only for 5MP Camera Version)
- 9. Dual Power Supply & Power cord hanger design
- 10. Warranty
1. Before Use
1-1. Operation
- As the microscope is a precision instrument, always use it with care. Avoid dropping or shaking during operation. Make sure the microscope is placed on a flat surface.
- Do not put the microscope in direct contact with the sun. Do not expose the microscope to high temperature, damp, or dusty environment.
- When moving the microscope, use both hands: typically one hand under the base and one hand on the arm (stand) of the microscope, and set it down carefully.
CAUTION:
It will damage the microscope if you hold the microscope either by the stage, focusing knob, or head.- Be sure the microscope is unplugged before replacing the bulb or fuse and wait until the bulb has cooled down.
- This microscope has a built-in voltage transformer and can be used with a wide voltage range from 110 to 240V.
- Only use the power cord supplied with the microscope.
1-2. Maintenance
- Wipe the lens gently with a soft lens tissue. After every use with oil or fingerprints, clean the lens surfaces with lens paper or lens paper moistened with a little rubbing alcohol. If you do not have lens paper, use a lint-free towel or cloth.
CAUTION:
Alcohol is flammable. Do not place this chemical near fire or fire-causing sources. Use this chemical in a ventilated place.- Do not use organic solutions to wipe the surfaces of other components.
- If the microscope comes in contact with liquid, turn off the power immediately and wipe it dry.
- Never disassemble or service the microscope yourself.
- After using, cover the microscope with a dust cover.
2. Diagram
VB-M006-B Microscope Diagram
This diagram shows the Vabiooth VB-M006-B microscope with the following labeled parts:
- Eyepieces: The parts you look through.
- 360 Degree Rotating Head: Allows the head to be rotated.
- Handle: For carrying or moving the microscope.
- Objective: Lenses located on the revolving nosepiece.
- Fine Focusing Handwheel: For precise focusing.
- 2-layer Mechanical Stage: Platform for holding and moving the specimen slide.
- LED Light Source: Provides illumination from below the stage.
- Light-control Knob: Adjusts the brightness of the LED light.
- Power Button: Turns the microscope on and off.
VB-M006-C Microscope Diagram
This diagram shows the Vabiooth VB-M006-C microscope, similar to the VB-M006-B, with the addition of a Camera attached to the head.
VB-M006-M Microscope Diagram
This diagram shows the Vabiooth VB-M006-M microscope, similar to the VB-M006-B, with the addition of an LCD Screen attached to the head.
3. Assembly
3-1 Install the Objectives
Install the objectives into the microscope nosepiece, starting from the lowest magnification to the highest, in a clockwise direction.
3-2 Insert the Eyepieces
- Remove the eyepiece tube covers.
- Insert the eyepieces into the tubes completely.
3-3 Install plug
Insert one side of the power plug into the back socket of the microscope.
4. Operation
4-1 Turn on
- Insert the power plug into an outlet and turn on the microscope.
- Adjust the light adjustment knob until you achieve the suitable brightness. (Refer to Figure 1, which shows the light control knob).
4-2 Set the specimen slide
- Push the arm of the specimen holder back.
- Insert the slide with the cover glass facing up, and then allow the arm of the specimen holder to move forward. (Refer to Figure 2, which illustrates placing the specimen slide).
- Rotate the X and Y-axis knobs on the mechanical stage to move the specimen to the center, aligning it with the objective.
4-3 Adjust focus
- Move the 4X objective into the light path.
- Rotate the coarse-focusing knob until an image appears.
- Turn the fine-focusing knob to make the specimen clearer. (Refer to Figure 3, which indicates the focus knobs).
Note: When using the 4X and 10X objectives, open the aperture diaphragm and field diaphragm to their widest positions.
4-4 Adjust tension of the focus
If the coarse focus knob is difficult to turn or the stage drifts downwards by itself, adjust the tension adjustment ring. Turn clockwise to tighten the controls or counterclockwise to loosen them. (Refer to Figure 4, showing the tension adjustment ring).
4-5 Adjust the interpupillary distance
When observing with both eyes, hold the bases of the eyepieces and rotate them around their axis until you see a single, unified field of view. (Refer to Figure 5, showing eye distance indication). The numbers on the left and right eyepiece bases should align to indicate the interpupillary distance.
4-6 Adjust diopters
Rotate one eyepiece to the "0" position using the white thread. Focus on a specimen clearly using only that eyepiece (this is for your dominant eye). Then, observe with the other eyepiece and rotate its diopter adjusting ring until the image is clear in that eyepiece as well. (Refer to Figure 6, illustrating diopter adjustment).
4-7 Adjust diaphragm
The diaphragm controls the amount of light entering the condenser, which can strengthen the image. For the clearest image, adjust the diaphragm so its image is just on the edge of the field of view.
4-8 Adjust aperture diaphragm
The aperture diaphragm determines the illumination's numerical aperture (N.A.). Matching the illumination N.A. with the objective's N.A. provides better resolution and contrast. If contrast is low, adjust the condenser's N.A. to 70%-80% of the objective's N.A. Adjust the upper diaphragm ring until the image appears as shown in Figure 7. For example, with a 40X objective (N.A. = 0.65), set the aperture diaphragm to approximately 0.52 (0.65 * 0.8). (Refer to Figure 8 for diaphragm adjustment).
4-9 Use the oil objective
Follow these steps for using the 100X oil objective:
- Use the 40X objective to focus on the specimen.
- Place a drop of immersion oil on the specimen. (Refer to Figure 9, showing oil position).
- Rotate the nosepiece to place the 100X objective (oil) into the light path. Use the fine focusing knob to focus.
Important: Ensure there are no air bubbles in the oil.
To remove air bubbles:
- Remove the eyepieces. Open the aperture and field diaphragms fully. Observe the edge of the objective from the tube without the eyepieces.
- Slightly rotate the nosepiece and swing the oil objective a few times to remove air bubbles.
After using the oil objective, clean the 100X lens with clean lens paper and rubbing alcohol if the oil does not come off easily.
Using the Filter
Filters can soften the background. A frosted filter is included. A polarizer can also be placed in this position when using the optional simple polarizing set.
5. Specification
5-1 VB-M006 Microscope General Specifications
VB-M006 Feature | Details |
---|---|
Eyepieces | WF10X, WF16X, and WF25X eyepieces |
4 Objective lenses | 4X, 10X, 40X (Spring), 100X (Spring, Oil) |
Magnification levels | 40X, 64X, 100X, 160X, 250X, 400X, 640X, 1000X, 1600X, 2500X |
Interpupillary distance | 55-75mm |
Adjustable ocular diopter | +/-5dp |
Head | 30 degree inclined, 360 degree rotatable trinocular head |
Mechanical stage | Mechanical stage with rack stop, 110x120mm |
Mechanical stage travel range | 70x30mm |
Abbe condenser | NA 1.25 Abbe condenser with iris diaphragm |
Knobs | Coaxial coarse and fine focus knobs |
LED light | Transmitted illumination with 1W LED bulb, adjustable brightness |
Power supply | Type-C 5V/1A 100-240V power input by AC adaptor or 3AA power supply, low voltage input, safe and power-saving, portable |
6. Troubleshooting
Optics
Symptom | Cause | Remedy |
---|---|---|
(1) The lamp is bright, but the light in the field of view is not bright enough. | Field diaphragm is not large enough. Condenser is too low. Condenser is not centered. | Enlarge the field diaphragm. Adjust the condenser. Center the condenser. |
(2) The side of the field of view is dark or not even. | The nosepiece is not in the right position. Stain or dust has accumulated on the condenser, objective, eyepieces, and base lens. Condenser adjustment needed (see step 3-7) | Turn the nosepiece into the right position. Clean the lens. Center the condenser. |
(3) Stain or dust is observed in the field of view. | Stains have accumulated on the specimen. Stains have accumulated on the lens. | Clean the specimen. Clean the lens. |
(4) Unclear image | No cover glass on the specimen slide. The cover glass is not standard. The cover glass faces down. The immersion oil has accumulated on the dry objective. The immersion oil is not used for oil objective 100XR. Air bubble in the immersion. Use wrong immersion oil. | Add the cover glass. Use a standard cover glass with thickness 0.17mm. Put the cover glass to face up. Clean thoroughly. Use immersion oil. Get rid of the air bubble. Use correct one. |
(5) One side of the field of view is dark or the image moves while focusing. | The aperture is not opened correctly. Stain or dust has accumulated on the lens in the inlet of the head. The condenser is not in the right position. The specimen slide is not fixed. The nosepiece is not in the right position. Condenser is centered incorrectly. | Adjust the iris diaphragm. Clean the lens. Adjust the condenser. Fix with clips. Turn the nosepiece into the right position. Center the condenser. |
(6) The eyes feel tired easily. The right field of view doesn't superpose with the left. | Interpupillary distance is wrong. Diopter adjustment is wrong. The eyepiece for the right is different from the left. | Adjust the interpupillary distance. Adjust the diopter. Use the same eyepieces. |
Mechanics & Electrical
Symptom | Cause | Remedy |
---|---|---|
(1) Can not get the objective focused. | The cover glass faces down. The cover glass is not standard. | Put the cover glass to face up. Use a standard cover glass with thickness 0.17mm. |
(2) The objective touches the cover glass while turning the nosepiece. | The cover glass faces down. The cover glass is not standard. | Put the cover glass to face up. Use a standard cover glass with thickness 0.17mm. |
(3) Coarse focusing knob is too tight. | Tension knob is too tight. | Loosen a little. |
(4) Stage declines itself and can't stay in the focus plane. | Tension knob is too loose. | Tighten a little. |
(5) Coarse focusing knob can't rise. | The limit stop knob is locked. | Loosen the knob. |
(6) Coarse focusing knob can't decline. | The base of the condenser is too low. | Raise the base. |
(7) Can not move the slide smoothly. | The slide is not fixed correctly. The movable specimen holder is not fixed properly. | Adjust it correctly. Adjust it correctly. |
(8) The image moves obviously when touching the stage. | The stage is fastened incorrectly. | Fasten the stage correctly. |
Electrical: (1) The bulb does not work. | No power supply. The bulb is not inserted correctly. | Check the connection of the power cable. Insert it correctly. |
(2) The bulb burnt out usually. | The bulb burnt out. | Replace it. |
(3) The field of view is not bright enough. | Use a wrong bulb. The use of light adjustment knob is wrong. | Replace with a correct one. Adjust correctly. |
(4) The bulb flickers or the brightness is not stable. | The bulb will burn out soon. The wire doesn't connect all right. | Replace with a new one. Connect correctly. |
7. How to install the monitor or camera?
To install a monitor or camera:
- Rotate the head of the tube counterclockwise; the bottom of the tube is twisted onto the machine.
- The tube will then divide into two parts: one for the 23.2mm ocular port or tube, and the other for the C-Mount.
The monitor fits for the 23.2mm tube, while the camera is typically C-Mount.
Monitor Operation:
- Turn on the power button.
- Select the AV1 channel.
- Use the monitor's "M" button to adjust BRIGHTNESS, CONTRAST, COLOUR, ZOOM, TCON, and LANGUAGE.
For instructional videos, visit the "Vabiooth" YouTube channel.
8. Microscope Camera (Only for 5MP Camera Version)
Features:
- High-speed USB2.0 interface, up to 480Mb/s.
- Large area array CMOS progressive scan image sensor.
- Supports static and dynamic image capture.
- Adjustable exposure time.
- 8-bit uncompressed raw image data, software implementation Bayer color conversion, plug-and-play installation.
- Supports WIN8/SP4, XP, VISTA, WIN7, WIN10, and Mac operating systems.
- Stable and reliable image performance after rigorous testing.
This camera is widely used in pattern recognition, industrial testing, medical microscopy, traffic management, machine vision, and various other fields requiring image acquisition.
It features a C-Mount eyepiece interface (adapter) designed for the microscope.
Camera Specification:
- Image sensor: 1 / 2.5" color CMOS image sensor
- Pixels size: 2.2 µm × 2.2 µm
- Spectral response: 400 nm to 1000 nm
- Scan mode: Progressive scan
- Sensor type: Pure hardware 5 megapixel image sensor, no compression, no interpolation
- Resolution: 640x480, 1280x1024, 1600x1200, 1920x1080, 2048x1536, 2592x1944
- White Balance: Auto/Manual
- R.G.B value setting: Independent
- Auto Exposure Control: Auto/Manual
- Image output: USB2.0, 480Mb/s
- Operating temperature: 0°C - 50°C
The kit includes a software CD with imaging application and calibration slide for measurement accuracy. If your computer lacks a CD drive, the software can be downloaded from: https://bit.ly/3XEZvxX
9. Dual Power Supply & Power cord hanger design
The microscope offers dual power options:
- 3AA Power Supply: Portable and does not require plugging in. (Illustrated by images showing battery compartment and batteries).
- Type-C 5V/1A Power Input: Uses a 100-240V AC adapter. This provides low voltage input, which is safe and power-saving. (Illustrated by images showing AC adapter connection).
10. Warranty
Warranty information is not detailed in the provided document pages.