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User Manual for dynamic BIOSENSORS models including: AS-2-Rc-lfs v5.1, heliX plus Adapter Strand 2, Adapter Strand 2, Strand 2
File Info : application/pdf, 4 Pages, 2.02MB
DocumentDocumentheliX+ User Manual ADAPTER STRAND 2 with red dye Rc and prehybridized with ligand strand Dynamic Biosensors GmbH & Inc. AS-2-Rc-lfs v5.1 Key Features · Adapter strand 2 for functionalization of heliX® Adapter Chip Spot 2. · Compatible with heliX® Adapter Chip. · Includes Adapter strands for 50 regenerations. · Ideal for MIX&RUN sample preparation. · Adapter strand 2 carries a hydrophobic red dye (Rc) with a neutral net charge. heliX® Adapter Chip Overview 2 spots with 2 different anchor sequences for DNA-encoded addressing. Product Description Order Number: AS-2-Rc-lfs Table 1. Contents and Storage Information Material Adapter strand 2 - Rc - lfs Cap White Concentration Amount 200/250 nM 5 x 200 µL For research use only. This product has a limited shelf life, please see expiry date on label. To avoid many freeze thaw cycles please aliquot the nanolever. Buffer TE40 [1] Storage -20°C AS-2-Rc-lfs v5.1 www.dynamic-biosensors.com Page 1 of 3 Preparation | MIX&RUN In-solution hybridization of adapter and ligand strands: 1. Mix Adapter strand 1 - Rc (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v). 2. Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization. 3. Mix solution of step 2 and Adapter strand 2 - Rc - lfs (200 nM) at 1:1 ratio (v/v). Solution is ready to use for biochip functionalization. Stability of the solution is related to the stability of the ligand molecule. Table 2. Additional material for functionalization of spot 1 and reference spot 2. Material Adapter strand 1 - Rc Ligand strand carrying the conjugated ligand Concentration 400 nM 500 nM Buffer TE40 [1] PE40 [ 2] Related Product Name Adapter strand 1 with red dye Rc heliX® Amine Coupling Kit 1 Order No AS-1-Rc HK-NHS-1 Example Required volume for 3 functionalizations: 100 µL with a final concentration of 100 nM. Vial 1 Adapter strand 1 - Rc (400 nM) 25 µL Conjugated Ligand strand (500 nM) 25 µL Vial 2 Adapter strand 1 - Rc - lfs (200/250 nM) 50 µL After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution. AS-2-Rc-lfs v5.1 www.dynamic-biosensors.com Page 2 of 3 Contact Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 Woburn, MA 01801 USA Order Information order@dynamic-biosensors.com Technical Support support@dynamic-biosensors.com www.dynamic-biosensors.com Instruments and chips are engineered and manufactured in Germany. ©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved. [1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 M EDTA, 50 M EGTA [2] If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet. AS-2-Rc-lfs v5.1 www.dynamic-biosensors.com Page 3 of 3