heliX+ DNA Enzyme Activity Kit User Manual

Key Features

• ssDNA template (48 + 32 bp) for functionalization of heliX® Adapter Chip on Spot 1.
• dsDNA template (48 + 32 bp) for functionalization of heliX® Adapter Chip on Spot 2.
• Compatible with heliX® Adapter Chip.
• Includes Adapter strands for 100 regenerations.
• This DNA template carries a moderately hydrophilic red dye (Ra) with a single positive net charge.

heliX® Adapter Chip Overview

The heliX® Adapter Chip features two distinct spots, each with a different anchor sequence designed for DNA-encoded addressing. Spot 1 is functionalized with an ssDNA template (Adapter strand 1 - nf), while Spot 2 is functionalized with a dsDNA template (cTemplate Adapter strand 2 - nf). These anchor sequences are fixed to the chip surface.

Product Description

Order Number: HK-EA-1

Table 1. Contents and Storage Information

[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA

For research use only. This product has a limited shelf life; please see the expiry date on the label. To avoid multiple freeze-thaw cycles, please aliquot the nanolever.

Preparation

IMPORTANT: Both Adapter strands are already pre-hybridized. Adapter strand 1 is pre-hybridized to the Template-32-P-Ra DNA strand, leaving the upper part as ssDNA. cTemplate-Adapter strand 2 is pre-hybridized to the Template-32-P-Ra DNA strand, leaving Spot 2 completely as dsDNA.

To prepare the solution for biochip functionalization, mix the Adapter strand 1 - nf / Template-32-P-Ra DNA (200/250 nM) and the cTemplate Adapter strand 2 - nf / Template-32-P-Ra DNA (200/250 nM) in the same vial at a 1:1 ratio (v/v).

Useful Order Numbers

Table 2. Order Numbers

Assay Setup in heliOS

This kit is designed for studying the enzymatic activity of nucleic acid modifying enzymes such as polymerases, reverse transcriptases, and helicases.

To set up an assay, navigate within the heliOS software: go to heliOS > create a New Assay Workflow > add Custom Assay > load Enzyme Binding and Activity. Modify the parameters as needed and run the assay. Suggested assay parameters, including flow rates, functionalization time, and LED power, are available within the heliOS assay setup.