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Instruction Manual for hygiena models including: BAX-Q7 Molecular Pathogen Detection System, BAX-Q7, Molecular Pathogen Detection System, Pathogen Detection System, Detection System
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DocumentDocumentBAX® System Q7 Ready Reference for Standard PCR Assays STEP 1: PREPARATION Add 150 L protease to 12 mL lysis buffer 150 µL Add 200 µL lysis reagent to cluster tubes 200 µL Ensure thermal blocks are pre-heated to 37°C or 55°C, and 95°C prior to use Ensure cooling blocks are stored at 2 8°C prior to use STEP 2: LYSIS Transfer 5 L* enriched samples to cluster tubes 5 µL *For E. coli O157:H7 use 20 µL STEP 3: PCR Create rack file, turn on cycler, and initialize Lysis reagent can be stored at 2-8°C for up to two weeks Heat cluster tubes (First Stage) 37°C for 20 minutes: Cronobacter 20 37°C E. coli O157:H7 Salmonella 55°C for 60 minutes: 0:60 Genus Listeria 55°C L. monocytogenes Heat cluster tubes (Second Stage) 95°C for 10 minutes: All targets Cool cluster tubes for a minimum of 5 minutes in cooling block 95°C Unopened processed lysates can be stored at 2-8°C for up to two weeks Arrange PCR tubes in PCR cooling block with black carrying tray Hydrate PCR tablets with 50 µL lysate from cooled lysates 50 µL On software, click next, place PCR tubes in Q7 cycler and run program Review results on screen Negative Positive Indeterminate Signal error For technical support contact us at diagnostics.support@hygiena.com or by visiting www.hygiena.com/technical-support-request INS2032 REVC