User Guide for fluidion models including: V1.5, V1.6.40, V1.6.41, V1.6.44, V1.6.45, ALERT One Handheld Microbiology Analyzer, ALERT One, Handheld Microbiology Analyzer, Microbiology Analyzer, Analyzer

ALERT One

Feb 11, 2025 — Download data Send datasets to serial port (use app to process). Set Threshold1 Adjust the risk thresholds. Date and Time Change device date and time.

ALERT One - fluidion.com

ALERT One Handheld Microbiology Analyzer 22 www.fluidion.com Prepare vial and reagent Prepare sample and reagent (p.2) Mix thoroughly 1 Insert vial in the device Remove analyzer cap


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ALERT One Quick Start Guide V1.5.2
ALERT One
Handheld Microbiology Analyzer V1.5

Need help ? Email support@fluidion.com

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Quick Start Guide

ALERT One
Handheld Microbiology Analyzer

Prepare vial and reagent 1 Prepare sample and reagent (p.2)
Mix thoroughly
Insert vial in the device 2 Remove analyzer cap
Insert vial fully Replace analyzer cap

3 Power the analyzer
Requirements: USB-C 5V DC, 15W Use supplied adapter or battery pack Battery must have trickle charge
capability activated

4

Start the measurement Navigate to Measure menu

Follow instructions below

Menu Navigation
Press Press & Hold (1s) Press & Long Hold (5s)
Starting a Measurement


X



Scroll Up

Scroll Down

Cancel / Back

OK / Validate

Advanced Data Display* * While performing a measurement, or in the History menu



 

Wait (2-14h) for measurement to end. Do not tilt !
E. Coli concentration per 100mL and corresponding risk level*

Adjust water sample tem per atur e

.. ... .

- - - - -

__ __ __ __ ___ ___ ___ ___

*Note: Risk thresholds are custom defined in the Settings Menu

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Exploring Data History


ALERT One
Handheld Microbiology Analyzer
Na vigate between mea sur emen ts using  and 

Settings Menu





Volume(ml): 50

Download data Send datasets to serial port (use app to process) Set Threshold1 Adjust the risk thresholds

Date and Time Change device date and time

Device Info Display serial number and firmware versions Clear Memory2 Delete all measurements from device memory

Sound

Toggle sound alerts On or Off

Display T.C. Calib1 Volume (ml)1

Toggle display of Coliform results On or Off Select a specific calibration Set the water sample volume

1 Will affect future measurements

2 Will result in data loss

Advanced Data Display: Long Press (5s) 

during measurement or in History Menu





Fluorescence curve (E. coli signal)

Absorbance curve (Coliform signal)

Raw signals and algorithm version

Available calibrations for use with ALERT One*

· Not all may be present. New calibrations under constant development.

1.6.40 E.coli TC River - Plastic v2 (EV2024)

Inquire with Fluidion for available upgrades. For freshwater (river, lake)

1.6.41 E.coli TC Seawater- Plastic v2 (EV2024)

For seawater (ocean, brackish)

1.6.44 Enterococci River - Plastic (ENTRP-ALM2024)

For freshwater (river, lake)

1.6.45 Enterococci Sea - Plastic (ENTSP-ALM2024)

For seawater (ocean, brackish)

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ALERT One
Handheld Microbiology Analyzer

Sample and Reagent Preparation*

*Shown for single-use plastic vial. Glass vials currently not supported. Does not apply to Enterococci freshwater measurements ­ inquire with Fluidion for protocol details

Freshwater samples

Seawater samples

Add

Add 50mL of

bioreagent

freshwater sample (to molded line)

Add bioreagent

Add 27mL of Add 9mL of sterile water seawater sample (to first line) (to second line)

Sample and vial disposal procedure
Dispose used vial as biological waste according to local regulations. If available, use appropriate biological waste collection services. Only when no biological waste collection service available, follow the vial disinfection procedure below:
Add 1ml of concentrated liquid bleach to vial containing sample. Close, shake, wait 15 minutes. Pour content in sanitary drain. Dispose of singleuse plastic vials as trash, or, if available, recycle as polypropylene (PP)
Important Safety Information:
· Read the reagent Material's Safety Data Sheet (MSDS) before use. · Wear protective gloves when dealing with potentially contaminated samples. · Protect clothing from bleach projections to avoid staining. · Keep instrument and battery dry and protected from the elements · Ensure that reagent is within its validity period. · Do not leave device in direct sunlight. Max. ambient temperature: 37oC. · Do not tilt the device while performing a measurement. Alarm will sound.

Product Warning
The reagents used with this product cause rapid multiplication of E.coli and other potentially harmful biological substances. Used samples and reagents should never be disposed directly into the environment or drinking water sources. This product and all associated reagents and consumables should never be used or accessed by unsupervised children.

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References