Introduction to the System
The Illumina NextSeq 1000 and NextSeq 2000 systems offer a targeted approach to Next-Generation Sequencing (NGS). These application-focused systems integrate Illumina's sequencing technology into a cost-effective, desktop instrument, providing enhanced accessibility and reliability. Key features include onboard denaturation and dilution, an integrated imaging module, and fluidics components within consumables for simplified maintenance.
The systems support a streamlined workflow, starting with the loading of single-use cartridges containing pre-filled reagents. Libraries and flow cells are loaded directly into the cartridge, which is then placed into the instrument. RFID integration ensures accurate tracking throughout the process.
The accompanying software suite controls instrument operation, manages system settings, and facilitates sequencing run setup and monitoring. Real-Time Analysis (RTA3) handles image analysis and base calling during the run, while the Universal Copy Service efficiently transfers sequence output files to BaseSpace Sequence Hub or designated output folders.
Key Features and Capabilities
- Simplified Workflow: Load all necessary reagents in a single cartridge.
- Integrated Imaging: Imaging module is built into the system.
- User-Friendly Interface: Control software provides intuitive operation.
- Flexible Operation Modes: Supports Cloud, Hybrid, Local, and Standalone modes for diverse research needs.
- Advanced Analysis: Utilizes DRAGEN technology for efficient data analysis.
Resources and Support
For detailed specifications, datasheets, application notes, and other related products, please visit the Illumina website's NextSeq 1000/2000 System Support page. This page provides access to additional system resources, including software updates, training materials, and compliance information.
