TPP TubeSpin® Bioreactor: Instruction for Use

Product Description

The TPP TubeSpin® bioreactor is designed for high-throughput screening, cultivation, and optimization processes of prokaryotic (aerobic bacteria) and eukaryotic (yeasts, algae, HEK, CHO, Sf-9) suspension cells. It is available with a filter screw cap featuring 5 or 10 openings covered by a PTFE filter membrane. The bioreactor is intended for single-use only.

Important Notes and Safety Precautions

• Handle biological material in accordance with national regulations and use appropriate protective clothing.
• Adhere to aseptic working practices throughout all procedures.

Instructions for Use

1. Inspect the packaging material and the product for intactness. Use only faultless material.
2. Verify the expiry date (EXP) on the label and packaging. Ensure products have a valid EXP.
3. Open the packaging in a sterile environment and remove a complete system for use.
4. Open the bioreactor and fill it with the appropriate medium and inoculum according to your laboratory routine. Refer to the technical data for optimal filling quantities.
5. Close the filter screw cap with ventilation holes. Continuous gas exchange is facilitated through the integrated hydrophobic membrane (0.22 μm).
6. If aeration/gas exchange needs to be reduced, individual openings may be closed using lab-appropriate labels or tape.

Cultivation and Shaking

• Cultivate: Shake cells in appropriate incubation shakers, adhering to the specific cultivation conditions for the cells (temperature, CO2 and humidity control, shaking frequency).
• Shakers: Follow the safety instructions and operating instructions provided by the shaker manufacturer.

Centrifugation

• Centrifuging: It is recommended to use suitable rotors or centrifuge adapters. Comply with the safety instructions of the centrifuge manufacturer.
• The mechanical strength of the tubes during centrifugation is influenced by:
  • Shape and material
  • Accuracy of fit in the centrifuge adapter
  • Temperature, centrifugation time, and g-force
  • Chemical and physical properties of the centrifuged material
  • Rotor type (fixed-angle or swing-out rotor)
• Conduct a test run with your chosen parameters before routine use.

Storage

• Store before use at room temperature, protected from UV light.
• For storage in sub-zero temperatures, transfer samples to TPP cryo tubes. If storing the TubeSpin® bioreactor in a sub-zero temperature range, TPP recommends testing the product beforehand under your specific conditions.
• TPP assumes no liability for damage caused by, during, or due to the use of centrifuge tubes in freezing processes.

Remarks and Recommendations

• The graduation markings serve as a guide for filling quantity and are not absolute values.
• Alcohol 90% with mechanical stress (rubbing) may dissolve or blur the white print.

Technical Data

Materials

Dimensions and Specifications

Openings / Openings (mm)

Additional Information

Instructions for use, chemical resistance lists, and quality certificates for the respective products are available for download on the homepage www.tpp.ch.