LMGP-502 Gradient PCR Machine
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Specifications
- Temperature Range: 0.1 to 30°C
- Features: Semiconductor technology, Touchscreen Display,
Block/Tube Temperature Control, Program Pause Function, USB
Connectivity
Product Usage Instructions
1. Safety Measures
The following fundamental safety precautions must be followed
during all handling, maintenance, and repair of this
instrument:
- Designed for normal indoor use only.
- Avoid attempting to open or repair the instrument to prevent
electric shock. All repairs should be done by professional
personnel. - Ensure the supply voltage matches the recommended voltage
before connecting the power supply.
2. Introduction
The Gradient PCR Machine LMGP-502 is designed for various
applications in Molecular Biology, Genetics, Clinical Diagnostics,
Biotechnology & Research Labs for DNA/RNA amplification,
sequencing, genetic disorder analysis, gene expression studies, and
mutation analysis.
3. Features
- Semiconductor technology for efficient performance.
- Touchscreen Display for easy operation.
- Block/Tube Temperature Control for precise temperature
regulation. - Program Pause Function for flexibility in running
programs. - USB Connectivity for easy data transfer.
4. Installation
Follow the provided installation guidelines in the manual to set
up the Gradient PCR Machine LMGP-502 correctly.
5. Operations
8.1 Operation Guide
8.1.1 Start Up
After powering on the instrument, press the power switch to
illuminate the LCD screen and proceed to the main menu
interface.
8.1.2 Run File Operation
The running file refers to the PCR amplification program. Follow
these steps:
- Access the File Library by clicking on the File icon on the
main interface. - To export a file, insert a USB disk before powering on the
instrument and follow the instructions on the screen. - Create a New File by clicking on the New button and set
temperature and time parameters using the numeric keypad.
FAQ
Q: Can this PCR machine be used for DNA sequencing?
A: Yes, the Gradient PCR Machine LMGP-502 is suitable for DNA
sequencing among other applications in Molecular Biology labs.
Q: Is it safe to open the instrument for maintenance?
A: No, it is not safe to open or repair the instrument yourself.
All maintenance should be carried out by professional personnel to
avoid risks of electric shock.
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Operational Manual
Gradient PCR Machine LMGP-502
www.labmate.com | info@labmate.com
Sr. No 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
Gradient PCR Machine LMGP-502 Index
Title Safety Measures
Introduction Features
Specifications Applications Instrument Introduction Installation Operations Maintenance Troubleshooting
Page no 2 3 3 4 4 5 5 6 13 14
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Gradient PCR Machine LMGP-502
1. Safety Measures
The following fundamental safety precautions must be followed during all handling, maintenance, and repair of this instrument. Ignoring these instructions or the warnings found elsewhere in this manual may compromise the instrument’s safety features and intended functionality. This instrument is designed for normal indoor use only. Users should not attempt to open or repair the instrument themselves, as this may
lead to electric shock. All repairs should be carried out by professional personnel. Before connecting the power supply, ensure the supply voltage matches the
instrument’s rated voltage. Also, confirm that the power outlet’s load capacity is not less than what the instrument requires. If the power cord is damaged, it must be replaced with one of the same type and specifications. Do not place heavy objects on the power cord during operation and avoid positioning the cord in high-traffic areas. Always grasp the plug itself when plugging in or unplugging the power cord. Ensure the plug is fully inserted into the socket. Never pull the cord to unplug it. The instrument should be installed in an environment with low humidity and minimal dust, away from water sources, direct sunlight, and strong light. The area should be well-ventilated and free from corrosive gases or electromagnetic interference. Keep the device away from heat sources such as stoves and heaters. Do not operate the instrument in damp or dusty environments. Turn off the power when the instrument is not in use. If the device will not be used for an extended period, unplug it and cover it with a soft cloth or plastic sheet to prevent dust accumulation. Immediately unplug the instrument from the electrical outlet if the following occurs Liquid has spilled inside the instrument The instrument has been exposed to rain or water The instrument is malfunctioning, particularly if unusual noises or odors are detected. The instrument has been dropped, or its casing is damaged. There is a significant change in the instrument’s performance or functionality.
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Gradient PCR Machine LMGP-502 2. Introduction
Gradient PCR Machine LMGP-502 works within range of 4 to 99.9°C. Its high-speed heating and cooling rates ensure efficient thermal cycling. Its user-friendly interface enables real-time monitoring and seamless operation management. It features multiple login management and large data storage, for secure access and protocol retrieval. Our Gradient PCR Machine is ideal for molecular biology, diagnostics, and research settings.
3. Features
Semiconductor technology Touchscreen Display Block/Tube Temperature Control Program Pause Function USB Connectivity
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Gradient PCR Machine LMGP-502
4. Specifications
Model No Single Step Time Temperature Range Sample Capacity Gradient Gradient Temperature Uniformity Gradient Temperature Accuracy Gradient Temperature Range Gradient Temperature Difference Range Maximum Heating Rate Maximum Cooling Rate Temperature Uniformity Temperature Accuracy Temperature Display Resolution Hot Cover Temperature Range Maximum Steps of the Program Program Max. Cycle Number Time Increment/Decrement Temperature Increase/Decrease Insulation Temperature Program Storage Quantity Power Consumption Power Supply Dimensions Net Weight
LMGP-502 1 to 59m59s 4 to 99.9 0.2ml × 96 12 ±0.3 ±0.3 30 to 99.9
0.1 to 30
4.5/s 4/s ±0.25 ±0.20 0.1 30 to 110 30 99 to 599 599s -9.9 to 9.9 16 Greater than 100 600W 100 to 240V, 50/60Hz 205 × 280 × 160 mm 4.3kg
5. Applications
Gradient PCR Machine LMGP-502 is ideal for Molecular Biology, Genetics, Clinical Diagnostics, Biotechnology & Research Labs for DNA/RNA amplification, sequencing, genetic disorder analysis, gene expression studies and mutation analysis.
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Gradient PCR Machine LMGP-502
6. Instrument Introduction
Structure
Hot Lid Button Vent
LCD screen USB interface
Figure-1
Hot Lid Power outlet
Fuse
Figure-2
7. Installation
Working Condition Ambient temperature5°C ~ 30°C Relative humidity70% Power Supply100~240V AC 6.6~3.1A 50/60Hz
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Gradient PCR Machine LMGP-502
8. Operations
8.1Operation Guide 8.1.1 Start Up
After powering on the instrument, press the power switch to illuminate the LCD screen, which will display the welcome screen (see Figure 3). During this screen, the product name appears on the display. Once the welcome screen is complete, the instrument proceeds to the main menu interface (see Figure 4).
Figure-3
Figure-4 8.1.2 Run File Operation
The running file refers to the PCR amplification program, which is composed of temperature steps and cycle steps. Each file can include up to 30 steps. 1) File Library
Click the “File” icon on the main interface to open the file library screen. The file list appears in the left column, while the right side displays detailed information and a preview of the selected file. Functional buttons are located at the bottom of the screen. When a file is selected, it can be edited, deleted, or renamed (see Figure 5).
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Gradient PCR Machine LMGP-502
To export a file, insert the USB disk before powering on the instrument. Once the interface shows “USB Storage,” select the file and press this button to export it to the USB disk. Kindly restart the instrument after completing the operation.
Figure-5 2) Create a New File
Click “New” to enter the new file editing interface. The temperature values are displayed above the temperature curve, and the time values are shown below it. Click on either the temperature or time to set their parameters using the numeric keypad (see Figure 6). Use the “+Step” button to add temperature steps and the “+Cycle” button to add cycle steps. (A maximum of 30 steps is allowed per file.)
Figure-6 7
Gradient PCR Machine LMGP-502
Once the parameter settings are completed, click the “Return” button to open the save dialog box. Click “OK” to proceed, as shown in Figure 7(1). Enter the file name in the “Name” field, then click “OK” again (see Figure 7(2)). The system will return to the file library interface, and the new file will now be created and saved.
Figure-7(1)
Figure-7(2) 8.1.3 Edit File
1) In the file library, select the file you wish to edit and click the “Edit” button to enter the editing interface. The temperature values appear above the temperature curve, and the time values are displayed below it. Click on the temperature or time to set their parameters using the numeric keypad. To add temperature or cycle steps, click the “+Step” or “+Cycle” buttons. (The total number of steps cannot exceed 30).
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Gradient PCR Machine LMGP-502
Figure-8 2) To configure detailed settings for a specific temperature step, select the step
and click the “Option” button. You can adjust parameters such as “Temp,” “Time,” “+Temp/c,” “+Time/c,” and “Grads.” After completing the settings, click “OK” to return to the editing interface (see Figure 9). If you click “Return,” the interface will return without saving the updated parameters.
Figure-9 8.1.4 Running File
1) In the file library, select the desired file and click “Run” to enter the running interface. Use “Lid Off” or “Lid On” to control the hot cover. The panel on the right side of the graph displays the hot lid and module temperatures, the current step time, and the total run time. Click “Run” again to start executing the file (see Figure 10). 9
Gradient PCR Machine LMGP-502
Figure-10 2) While the file is running, a flashing temperature curve indicates the active step.
During the run, you can click “Pause” to temporarily halt the operation, “Continue” to resume, “Skip” to bypass the next step, or “Stop” to end the run. 3) Once the file completes its run time, the system will enter a low-temperature storage mode, and the “Total Time” will show as complete. Click “Stop” to end the operation. 8.2 Gradient Calculation 1) Click the “Calculator” icon on the main interface to access the gradient calculation screen (see Figure 11).
Figure-11 2) Click on the designated input fields to enter the module temperature and the
gradient range. Once the information is entered, click “OK” to automatically calculate the temperature for each sample well. Click “Return” to go back to the main interface.
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Gradient PCR Machine LMGP-502
8.3User Management click the “User” icon on the main interface to access the user list within the user management screen (see Figure12).
Figure-12 Click “Register” to open the registration interface. Enter the username, password, and other required details, then click “OK” to complete the registration process. Click “Login” to access the login screen. Enter the appropriate credentials to log in successfully. Note: Logging in as “Admin” allows the user to delete other user accounts. 8.4System Settings Click the “System” icon on the main interface to access the system settings screen (see Figure 13). Once the settings are configured, click “OK” to save them, or click “Return” to go back to the main interface.
Figure-12 11
Gradient PCR Machine LMGP-502
8.5Accessing Help and Importing Files Click the “Help” icon on the main interface to open the help screen (see Figure 13). Insert the USB drive before powering on the instrument. The interface will then display an “Import File” button–click it to switch to the import screen. Select the desired file and click the “Import” button to upload it to the current user. Use the “Refresh” button to update the file library. After completing the operation, restart the instrument.
Figure-13
Figure-14 12
Gradient PCR Machine LMGP-502
Figure-15
9. Maintenance
Regular Cleaning 1) Clean the holes in the base using a neutral soap solution. (Avoid using strong
alkalis, concentrated alcohol, or organic solvent solutions.) 2) Ensure there are no objects placed beneath the machine or near the left and right
cooling vents. Over time, dust may accumulate on the heat dissipation vents and should be removed promptly. This is essential for proper operation. 3) The module should be cleaned regularly. Accumulated reactant residues in the module cavity can affect temperature accuracy. It is recommended to wipe the cavity with a cotton cloth on a regular basis.
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Gradient PCR Machine LMGP-502
10. Troubleshooting
Error message Display Error Open:x Short:x Display screen not bright and abnormal Touch failure Block is not heated Block temperature is too high / too low Hot cover is not heated Hot cover temperature is too high Fan cannot rotate
Possible causes and corresponding counter measures
Sensor open circuit or short circuit
Hardware failure
Labmate Scientific Inc Email: info@labmate.com | Website: www.labmate.com
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Documents / Resources
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Lab mat LMGP-502 Gradient PCR Machine [pdf] Instruction Manual LMGP-502 Gradient PCR Machine, LMGP-502, Gradient PCR Machine, PCR Machine, Machine |