ACCURIS INSTRUMENTS MR9610 SmartReader UV-Vis Microplate Reader Instruction Manual

អរគុណសម្រាប់ការទិញasing our MR9610, SmartReader UV-Vis Microplate Reader. This user manual describes how the instrument works and includes an operation guide, please read it carefully before operation and keep for future reference.

ការត្រួតពិនិត្យបឋម

Please check the instruments as well as all accessories with the packing list when first received and unpackaged. If you find any missing, please contact your distributor or Accuris Instruments.

ការព្រមាន និងការណែនាំអំពីសុវត្ថិភាព

ព័ត៌មានសំខាន់ៗសម្រាប់ការប្រើប្រាស់ប្រកបដោយសុវត្ថិភាព

Users should have a clear understanding on use this instrument before operation. Please read this user manual carefully.

Please read the manual carefully and operate safely according to all guidelines.

This instrument is intended for Research Use Only

គន្លឹះសុវត្ថិភាព

The operation, maintenance and repair of the Instrument should comply with the basic guidelines and the warnings below.

ប្រើតែក្នុងផ្ទះប៉ុណ្ណោះ។

Warning: Biological contamination hazard. All samples for testing, quality control, and calibration should be regarded as infectious, and any parts that come into contact with samples should also be treated as infectious. Please wear gloves and appropriate safety gear when operating this device.

Before using the device, read the operation manual carefully. These instruments are designed for use in a laboratory environment. The device must be operated by skilled laboratory personnel with the appropriate training.

Warning: Avoid injury. Keep your body or any part of body at a safe distance (1 foot or more) from the instrument when running.

The operator should not open or perform any repairs on the Instrument without specific instructions from Accuris Instruments or an authorized agent of Accuris. Performing any unauthorized service repairs will result in loss of warranty coverage. Contact Accuris Instruments or an authorized distributor or agent in the event of any service issues.

មុនពេលបើកភ្លើង សូមបញ្ជាក់ថាវ៉ុលtage used meets the electrical requirements of the instrument as stated on the rating plate. If the electric cord is damaged, replace it with the same type.
Hold the socket firmly when you pull the plug from an outlet, and don’t pull the electric line only.

The Instrument should be put installed in an environment of standard room temperature, low dust, low humidity and away from direct sunlight. Also make sure there is adequate ventilation, no corrosive gas or strong magnetic field.

Always power off the instrument when you are finished using it. Unplug the power cord and cover the instrument with a cloth or plastic sheet to prevent excessing dust from entering the housing.

Pull the connector plug from the electrical outlet immediately and contact the vendor in the case of:
⚫ Liquid entering the housing.
⚫ Abnormal operation: such as any abnormal sound or smell.
⚫ The instrument is dropped or there is any damage to the housing.
⚫ Any malfunction

ជំពូកទី 1 សេចក្តីផ្តើម

The SmartReader UV-Vis is an automated microplate reader for ELISA assays. Through absorbance readings, it can determine the concentration, as well as positive or negative detection of antibody and antigen in samples when used with ELISA kits and solutions. The instrument can also be used for nucleic acid and protein quantification.

លក្ខណៈសំខាន់ៗ៖

1. 10-inch color touch screen.
2. Operating system allows acquisition, editing and saving of data.
3. Stand-alone system incorporates built-in processing, it can be optionally connected to a PC with the use of SmartReader software.
4. Graphical interface displays a representative plate for easy setting of well parameters: blank, sample, standards, positive/negative, quality control and multi-value comparisons.
5. Dual optical channels, one used for sample detection and one used for reference.
6. End-point method, kinetic readings, and spectral scanning can be performed.
7. Accepts plates with or without lids. Top heating prevents condensation.
8. ស៊ីណន អិលamp offers long life: 109 flashes (1,000,000,000), equivalent to approximately 1,300,000 plate reads.
9. Plate incubation feature allows accurate and uniform temperature control (≤0.5°C deviation between wells).
10. Top reading optical path with continual self-checking and automatic calibration option for monochromator wavelength and plate positioning.
11. Shaking function, time and speed are adjustable.
12. USB flash drive data export, for fast and easy data transfer.
13. Multi-user operation, data management for separate users.

ជំពូកទី 2 លក្ខណៈពិសេស

លក្ខខណ្ឌការងារ៖

Ambient temperature: 10ºC~40ºC
Relative humidity: 30%~80% (Non-condensing environment)
Power: AC 100-240V 50-60Hz 2A

ប៉ារ៉ាម៉ែត្រ៖

Chapter 3 Instrument Overeview

រចនាសម្ព័ន្ធ

ជំពូកទី 4 ការដំឡើង

ការត្រួតពិនិត្យបឋម

Each MR9610 is thoroughly tested before shipping, but please check it again when you receive the instrument and contact your local distributor or Accuris Instruments if:

• The outer package is damaged
• The outer package has any obvious moisture stains
• The outer package has marks of impact

After opening, please check the instrument and box contents.
Confirm that all ordered accessories have been included.
Check the instrument’s appearance for any damage.

ទីតាំងដំឡើង និងបរិស្ថាន

• Installation location: locate the instrument on a flat, dry, clean and stable surface, allow enough room on the front side so there is space for plate holder drawer to move in and out, and also keep 15cm / 6 inches of space behind and on the sides for any wires and air flow.
• បរិស្ថាន៖
  a. The environment for installation should not be excessively humid or dusty.
  b. Temperature should be within the range of +10°C ~ +40°C.
  c. Relative humidity should be within the range of 30% ~ 80% to avoid condensation.
  Note: KEEP THE INSTRUMENT AWAY FROM CORROSIVE GASSES AND LIQUIDS.

ជំហានដំឡើង

1. Place the instrument on a stable and level surface.
   Note: DO NOT loosen any screws or parts without first consulting with the Accuris Instruments Service Department or your authorized distributor. Doing so can cause damage and void the warranty.
2. Connect the instrument to an appropriate power outlet using the provided power cord.
   
   Note: One cord coming from the power adapter will fit to the input jack on the back of the instrument. Make sure to connect it according to the above picture. The other cord will connect to a standard electrical outlet (100VAC to 230VAC)
3. After connecting the power cords, switch the power button on the rear of the instrument to the ON position, the front panel will cycle through a start-up and self-test screen.
   Warning: Do not connect the instrument to a power outlet that is not properly grounded.

ជំពូកទី 5 ការណែនាំអំពីប្រតិបត្តិការ

Initial self-check

This chapter explains a standard protocol set up and operation, beginning with the self-check after power on. The following screen image will appear during the self-check:

The user login interface will appear after the self-check, see Fig 3. This allows login as the Admin, existing user, or click “Guest”.

Note: Please keep the Admin password recorded and in a safe place, or contact Accuris Instruments or your distributor if the Admin password is lost or forgotten.

After logging in, the Home Interface screen is displayed (Fig 4.)

The “Admin” button on the lower left is for the logout and login interface.

ការកំណត់ប្រព័ន្ធ

In the “Systems” section, users create system settings (time, date and language) according to their needs, for details see Fig 5.

ចំណាំ៖

1. The instrument must be restarted after the date and time settings are changed.
2. The Maintenance function is accessible only by authorized service representatives.
3. Click the “Home” button on top left corner to go to the main interface.

Protocol Management

Click “Absorbance” on the home interface to enter the layout interface (fig 6). This interface is composed of six sections: the navigation bar at the top, the sidebar, the main display area, the optional bar, setting type selection and the status bar at the lower right area.

Protocol name can be modified by clicking it directly.

The default name of a new protocol is the system time, and it can be modified manually. A pop-up window will appear to remind the user when a modified protocol has not been saved.

Save the current protocol to the protocol list.

Shortcut for exporting raw data to a flash drive in .csv format.

Open the Protocol folder for accessing saved protocols, sorting, deleting, importing, exporting, and renaming protocols. The Protocol list opens on the right side of the screen and can be closed by clicking on the left area.

When the protocol list is in the standard state, users can perform the below operations:

• Search: Enter a keyword to search the list.
• Sort: protocols can be sorted according to “Name”, “Date” and “State”. “demo1”, “demo2” and “demo3” are always displayed at the first three positions.
• Import: For importing protocols from a flash drive to the instrument.
• Rename: Change the name of a protocol.
• Save as: Save as a new protocol.
• Edit: “Edit” button is located on the top right corner, see Fig 7 to enter the edit state.
• Account: Protocols of other accounts can be checked, but this function is only available for the Admin account.

When the protocol list in the edit state, the below operations are available:

• Sorting: protocols can be sorted according to “Name”, “Date” and “State”.
• Checkbox: “▢” can used to select more than one for batch operation.
• Delete: delete selected protocols.
• Export: For exporting selected protocols to a flash drive.
• Cancel: Return to the standard state of the protocol list.

Reading a Microplate

After a protocol name is created, the next step is to set the parameters according to the experiment requirements.

Plate layout settings

The interface will automatically display the plate including the 384 well plate layout when creating a new protocol.

The plate layout menu allows you to choose 6 well-setting options, as well as a clear option. Choose the appropriate setting type, and the option bar will change accordingly, then click the corresponding wells on the main display area to finish the settings.

Note: the entire plate (all wells) can be selected by clicking the blank box at the upper left corner of the plate layout.

Blank: Set for a blank control during measurement. The average absorbance value of the blank wells is subtracted from the absorbance values the other wells after a plate read.

Standard: Used for creating the standard curve. After clicking “Standard”, the option bar will allow setting replicates and also calculations for quickly setting the standard concentrations, see Fig 9.

• Replicates: Use to set the same standard for multiple wells.
• Concentrations: After setting the concentration and unit of the first well, the protocol will calculate and set subsequent standards automatically according to the set operator and step size, or the values can be modified manually by clicking the corresponding wells.
• Example: If the set concentration is 125ng/μL, the operator is “×” (multiply) and the step size is “2”, the concentration of the first well will be 125ng/μL, the second well 250ng/μL (2 x 125ng/μl) and the third 500ng/μL (2 x 250ng/μL)… etc.

Quality control. Settings are the same as wells of standard samples ។

មិនស្គាល់ សamples. Users can set wells as unknown, and there in an additional “Factor” option if the unknown sample has been diluted. If a dilution has been used, set the value for the dilution factor “X. 1:X means the unknown sample was diluted X times.

Positive Control, users can set several wells as positive controls

Negative Control, users can set several wells as negative controls

Used for clearing the settings of the plate layout.

Note: The plate layout can be set up before or after the plate is read. For example, users can set all wells as “unknown”, read the absorbance values, then set up the layout of plate. The absorbance value data and layout are separate, users can create or modify a layout to analyze absorbance data that was captured in the past.

Note: the absorbance values of a test cannot be changed!

ការកំណត់ប៉ារ៉ាម៉ែត្រ

After completing the plate layout settings, click “Run” on the left side bar (see Fig 10.)

After clicking “Run” the lower window becomes a controller with the following functions:

• Incubator: The plate chamber can be set to a specified temperature, and the actual temperature is displayed.
• Wavelength: shows wavelength parameters of the current protocol.
• Shake: This function can be set to on or off.
• Progress bar: displays the running status of the current protocol.
• Plate in/out: control the motorized plate drawer to move it in and out, also there is also a physical control button on the front of the instrument.
• Start/Stop: Used to start or stop a plate reading. There is also a physical button on the front of the enclosure for start/stop.
• Shake: This setting is independent from the shaking function in settings menu, it is specifically used for plate defoaming.

In “Run” mode, the window on the right side allows choosing a specific measurement method: Endpoint, Kinetic or Spectrum, as well as reading parameters. See below table 2.

For wavelength selection, Endpoint and Kinetic are configured with four wavelengths (defaults are 405nm, 450nm, 492nm and 630nm), users can click on and modify these wavelength values manually, but must be within the range of 200nm~1000nm. Spectral analysis allows setting a wavelength range within 200nm~1000nm
A “Log” button appears at the top right corner of the plate layout window after the current protocol has been executed.
The “Log” is mainly used for recording the finishing time of each step of a protocol.
Note: Steps cannot be edited if a protocol has been executed, users can save it as a new one and then edit.

Reading a Plate

ចុច “” on screen or press “Plate in/out” on the front of the enclosure to open the plate drawer, load an ELISA plate on the plate holder, paying attention to the orientation of the plate so the A1 well is in the correct location. See Fig 11.

Click button “ ” on the display or press the “Start” button on the front of the instrument. If the protocol has already been run, a window will pop up to allow re-naming the protocol results file. Input a file name, click “ok” to run the protocol. The plate drawer will close, and during the reading process the screen will turn grey (see Fig 12) and all buttons will become inoperable with the exception of the stop button “ ”. The user can also press the “Stop” button on the front of the housing to stop a run.

ដំណើរការលទ្ធផល

The screen will stay in the “Run” interface after detecting samples and will display the original absorbance values measured. To view the analyzed results, switch to the “Result” interface by pressing the Result button on the side bar, see Fig 13.

Results are displayed according to the protocol settings and measurement method. The above Fig 13 shows results of an endpoint reading. Here, according to different types of detection modes, the Result is divided into three modes: Endpoint, Kinetic and Spectrum.

Endpoint result

As Fig 13 shows, data processing options for an endpoint result include: “Raw Data”, “Blank Subtraction”, “Basic Calculation”, “Standard Curve”, “Classification” and “Quality Control”.

• Raw data: displays absorbance values of each well. If the plate was read using multiple wavelength, the user can switch to different wavelengths by pressing the button “ ” at the top, middle of the main interface. Blank Subtraction: An average blank absorbance value is obtained per the wells set for “blank” samples. This average value is then subtracted from the absorbance values for each sampល្អ

ចំណាំ៖

1. The Blank Subtraction button is only available if blanks have been set in the protocol layout interface.
2. When a protocol layout is set with blank samples, the absorbance values of “Basic Calculation”, “Standard curve”, “Classification”, “Quality Control” and “Kinetic Analysis” all include the subtraction of the blank value.

• “Basic Calculation”: the four basic functions of “+”, “-”, “x”, “/” can be performed on the absorbance values at different wavelengths of the same well.
• “Standard Curve”: Based on the concentrations set for the standard wells and the corresponding measured absorbance values, the instrument will generate a standard curve and use this for sample concentration calculations, see Fig 14.
  
  If the curve fit is not closely matching the data points, the curve type can be changed in the “Parameters” window on the right of the interface. Below are the 8 curve fitting types available:
  ➢ Linear fitting
  ➢ Four Parameters Logistic (4PL)
  ➢ Quadratic polynomial
  ➢ Cubic polynomial
  ➢ Quartic polynomial
  ➢ Point-to-point
  ➢ Cubic Spline
  ➢ Logit/Log
  The absorbance values and concentration values of the standards are set to a curve. The SmartReader supports the following types of curve fittings that can be chosen to best fit the standard curve. When R2 value is closest to 1.0, this indicates an optimized curve fit.:
  ➢ Linear/Linear (Linear fitting of absorbance value and corresponding concentration)
  ➢ Linear/Log (Linear fitting of absorbance value and the logarithm of the concentration)
  ➢ Log/Linear
  ➢ Log/Log
  Note: In order to generate a curve, standard wells must be set up on the Protocol Layout Interface. Some curve types require a minimum quantity of standards. In the event of a “curve fit failure”, additional standards and concentration values may need to be included in the layout settings.
• Qualitative analysis: Samples can be qualitatively analyzed in relation to negative and positive references as set up in the plate layout. (see Fig 16). A formula is set up (on the right side of the interface), for calculating and marking positive “+ ”, weak positive “ L+”, or negative (no marking) sample wells automatically. Enter the appropriate values to create the formula for the “critical value”, “weak value”, and select if positive result will be > or < the critical value.
  
  Note: The Protocol layout interface must be set with at least one positive or negative well, or the button is not available.
• Quality Control: Click “Quality Control” button, (see Fig 16), the main display area will turn out to be a list, it displays status of the quality control wells which have set previously, the instrument will mark the samples according to conditions set in “Parameters”.
  
  Note: protocol layout interface must be set with “Quality” wells, otherwise, this button will be not available.

Kinetics

After a kinetic type assay, the results screen will display small graphs inside the squares instead of absorbance values (see Fig 17).

To see the detailed absorbance results for a specific well, select the target well, then click the “Graph” button at the top right to enlarge the kinetic curve (see Fig 18). Click “Back” to return to the main interface.

The “Calculation” window at the bottom offers four selections: “Raw Data”, “Blank Subtraction”, “Basic Calculation” and “Kinetic Analysis” (see Fig 18).
For functions and algorithms of “Raw Data”, “Blank Subtraction” and “Basic Calculation”, please see Section 5.1.
Clicking the “Kinetic Analysis” button, the window on the right side will offer different calculation options, (see Fig 19).
The calculations available include:
➢ Average/SD/CV%
➢ Integral
➢ Baseline Subtraction
➢ Select Single Reading
➢ Select Reading Range
➢ Maximum Rate
➢ Maximum (Peak)

ការវិភាគវិសាលគម

See Fig 20, select the target wells. click “Graph” button to enlarge the spectral curve, the absorbance of each wavelength shown on the curve. Users can click “Back” button to the graphic main interface.

Click “Spectral Analysis”, see Fig 21, for further analysis.
Calculations available:
➢ Spectral Maximum: read the maximum value greater than the threshold value within a set range.
➢ Spectral Normalization：set the spectral range, take the maximum absorption peak as 1, the remaining values will be converted into percentage based on this criterion.
➢ Ratio within the Spectrum: set two wavelength values λ1 and λ2, calculate the value of λ1/λ2.
➢ Select Wavelength Range：read measurement values within a set wavelength range.
➢ Select Single Wavelength ：read the measurement value of a single wavelength.

Description of each button on the upper right corner of the main display area:

Report exporting

Both processed data and raw data can be exported, click the “Report” button on the left side menu, see Fig 22.

Choose the desired format in the “File Type” window at the bottom, four formats are available:
◼ XLS
◼ CSV
◼ PDF
◼ TXT

Choose the content to export in “Output Content” on the right side, “√” will appear next to the selected content, then click “Output” to export data to a flash-drive.

បិទថាមពល

Remove ELISA plate from the Reader, then plate in the plate holder to the chamber. Turn off the power switch on the back of the instrument.

Chapter 6 Maintenance, storage, transportation

ថែទាំ

• Keep the installation environment dry and clean to prevent moisture, corrosion, and strong electromagnetic interference sources.
• The SmartReader Instrument is calibrated before it leaves the factory. There are no user-serviceable parts inside, so please do not attempt to open the housing. If any defects or operation problems arise, please contact your distributor or Accuris Instruments at ៨៦៦-៤៤៧-២១៩៤ or info@accuris-usa.com.
• Do not switch on and off the power abruptly, as this can damage the electronics.
• Make sure to connect the SmartReader to an appropriate power outlet (voltage and Hz) as stated on the instrument rating plate.
• បញ្ជីថែទាំ

ការផ្ទុកនិងការដឹកជញ្ជូន

• When not in use, storage the instrument at a temperature range -10℃～40℃, relative humidity less than 80%, without corrosive gas and with good ventilation.
• Prevent from heavy vibrations and humidity during transportation.

ជំពូកទី 7 ការដោះស្រាយបញ្ហា

ឯកសារ/ធនធាន

ACCURIS INSTRUMENTS MR9610 SmartReader UV-Vis Microplate Reader [pdf] សៀវភៅណែនាំ MR9610, MR9610 SmartReader UV-Vis Microplate Reader, MR9610, SmartReader UV-Vis Microplate Reader, UV-Vis Microplate Reader, Microplate Reader, Reader

ឯកសារយោង

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