Multichannel systems IFB-C Interface Board Multiboot System Tus neeg siv phau ntawv

Xyuas kom lub cuab yeej tsis raug tshav ntuj ncaj qha.
Tsis txhob cuam tshuam lub cuab yeej lossis muab tso rau sab saum toj ntawm lwm cov khoom siv hluav taws xob.

Tso cai rau kom muaj huab cua ncig ncig lub cuab yeej.

Installation ntawm Software:

Ua raws li cov lus qhia kev teeb tsa software muab los ntawm cov chaw tsim khoom.
Xyuas kom meej tias lub kaw lus ua tau raws li qhov yuav tsum tau ua yam tsawg kawg nkaus rau kev teeb tsa software.
Qhib lub software siv tus yuam sij daim ntawv tso cai los yog ua kom cov txheej txheem.

FAQs

Q: CMOS-MEA5000-System puas tuaj yeem siv rau kev kho mob?
- A: Tsis yog, cov khoom no tsis yog siv rau kev kho mob rau tib neeg. Nws yog tsim los rau kev tshawb fawb thiab kev sim ua haujlwm nkaus xwb.

Q: Kuv yuav ua li cas yog tias kuv ntsib teeb meem nrog lub cuab yeej?
- A: Yog tias koj muaj kev ua haujlwm tsis zoo uas tuaj yeem cuam tshuam kev nyab xeeb, nres siv lub cuab yeej tam sim ntawd thiab tiv tauj cov kws tshaj lij tsim nyog rau kev pab.

Q: Qhov twg kuv tuaj yeem nrhiav tau cov ntaub ntawv lav thiab lav rau cov khoom?
- A: Cov xwm txheej dav dav ntawm kev muag khoom thiab xa khoom, nrog rau cov ntaub ntawv lees paub thiab kev lav phib xaub, tuaj yeem pom hauv online ntawm http://www.multichannelsystems.com/sites/multichannelsystems.com/files/documents/TermsandConditions.pdf

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CMOS-MEA5000-System
USER MANUAL

CMOS-MEA5000-System · Tshaj tawm 20231220 · www.multichannelsystems.com

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

TSIS TXAUS SIAB
TSIS TXAUS SIAB
Cov ntaub ntawv hauv daim ntawv no yuav hloov pauv yam tsis tau ceeb toom. Tsis muaj ib feem ntawm daim ntawv no yuav raug muab luam tawm lossis xa tawm yam tsis tau sau ntawv tso cai los ntawm Multi Channel Systems MCS GmbH. Txawm hais tias txhua qhov kev ceev faj tau raug coj mus rau hauv kev npaj cov ntaub ntawv no, tus tshaj tawm thiab tus sau yuav tsis muaj lub luag haujlwm rau kev ua yuam kev lossis kev tso tseg, lossis kev puas tsuaj uas tshwm sim los ntawm kev siv cov ntaub ntawv uas muaj nyob hauv daim ntawv no lossis los ntawm kev siv cov kev pab cuam thiab qhov chaws uas yuav nrog nws. Nyob rau hauv tsis muaj qhov tshwm sim yuav tsum tus tshaj tawm thiab tus sau yuav tsum lav rau ib qho kev poob nyiaj los yog lwm yam kev lag luam puas tsuaj los yog raug liam tias tau tshwm sim ncaj qha lossis tsis ncaj los ntawm daim ntawv no.
© 2022 Multi Channel Systems MCS GmbH. Txhua txoj cai.
Luam tawm: 13.07.2022
Multi Channel Systems MCS GmbH Aspenhaustraße 21 72770 Reutlingen Lub teb chaws Yelemees Xov tooj +49-71 21-909 25 – 0 Fax +49-71 21-909 25 -11 sales@multichannelsystems.com www.multichannelsystems.com
Microsoft thiab Windows yog cov cim lag luam ntawm Microsoft Corporation. Cov khoom lag luam uas raug xa mus rau hauv daim ntawv no yuav yog cov cim lag luam thiab / lossis cov cim lag luam sau npe ntawm lawv cov neeg tuav khoom thiab yuav tsum tau sau tseg xws li. Tus tshaj tawm thiab tus sau tsis tau thov rau cov cim lag luam no.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

SAFETY THIAB WARRANTY

TSEEM CEEB TSHAJ PLAWS

Ceeb Toom: Nco ntsoov nyeem cov lus qhia hauv qab no ua ntej kev teeb tsa lossis siv lub cuab yeej thiab software. Yog tias koj tsis ua tiav txhua qhov kev xav tau hais hauv qab no, qhov no yuav ua rau muaj kev ua haujlwm tsis zoo lossis tawg ntawm cov khoom siv txuas nrog, lossis txawm tias raug mob hnyav.
Ceeb Toom: Ib txwm ua raws li cov kev cai hauv zos thiab cov cai. Tsuas yog cov neeg ua haujlwm tsim nyog yuav tsum raug tso cai ua haujlwm hauv chav kuaj. Ua haujlwm raws li kev soj ntsuam zoo kom tau txais txiaj ntsig zoo tshaj plaws thiab txo qis kev pheej hmoo.
Cov khoom tau raug tsim los rau hauv lub xeev ntawm kev kos duab thiab ua raws li kev lees paub kev ruaj ntseg engineering cov cai.
Cov cuab yeej siv tau nkaus xwb
siv rau nws lub hom phiaj; siv thaum nyob rau hauv ib tug zoo meej mob.
Kev siv tsis raug tuaj yeem ua rau mob hnyav, txawm tias raug mob hnyav rau tus neeg siv lossis cov neeg thib peb thiab kev puas tsuaj rau lub cuab yeej nws tus kheej lossis lwm yam khoom siv puas tsuaj.
Ceeb toom: Cov cuab yeej thiab software tsis yog siv rau kev kho mob thiab yuav tsum tsis txhob siv rau tib neeg. MCS tsis muaj lub luag haujlwm nyob rau hauv txhua rooj plaub ntawm kev ua txhaum cai.
Kev ua haujlwm tsis zoo uas tuaj yeem cuam tshuam kev nyab xeeb yuav tsum tau kho tam sim ntawd.
Hauv av
Cov khoom no yog grounded los ntawm grounding conductor ntawm lub hwj chim qaum. Txhawm rau kom tsis txhob muaj hluav taws xob poob siab, tus neeg xyuas pib hauv av yuav tsum txuas nrog lub ntiaj teb.
Orient cov khoom kom raug
Tsis txhob taw qhia cov cuab yeej kom nws nyuaj rau kev tswj cov khoom siv tsis sib xws.
Siab Voltage
Cov hlua hluav taws xob yuav tsum tau muab tso kom zoo thiab txhim kho. Qhov ntev thiab zoo ntawm cov hlua yuav tsum ua raws li cov kev cai hauv zos.
Tsuas yog cov kws tshaj lij tsim nyog tuaj yeem ua haujlwm ntawm lub tshuab hluav taws xob. Nws yog ib qho tseem ceeb uas yuav tsum tau ua raws li cov cai tiv thaiv kev sib tsoo thiab cov koom haum ntawm cov chaw ua haujlwm lav ris.
· Txhua lub sij hawm ua ntej pib, xyuas kom meej tias cov khoom siv hluav taws xob pom zoo nrog cov khoom tshwj xeeb. · Tshawb xyuas cov hlua hluav taws xob kom puas tsuaj txhua lub sijhawm hloov chaw. Cov hlua fais fab puas yuav tsum tau hloov tam sim ntawd thiab
tej zaum yuav tsis rov siv dua.
· Tshawb xyuas cov coj mus rau kev puas tsuaj. Cov hlau lead puas yuav tsum tau hloov tam sim ntawd thiab tej zaum yuav tsis rov qab siv dua. · Tsis txhob sim ntxig ib yam dab tsi ntse lossis hlau rau hauv lub qhov cua lossis rooj plaub. · Cov kua yuav ua rau luv luv lossis lwm yam kev puas tsuaj. Nco ntsoov khaws cov cuab yeej thiab cov hlua fais fab kom qhuav. Tsis txhob kov nws nrog ntub dej
tes.
Yuav tsum tau rau lub installation
Xyuas kom tseeb tias lub cuab yeej tsis raug tshav ntuj ncaj qha. Tsis txhob tso ib yam dab tsi rau saum lub cuab yeej, thiab tsis txhob muab tso rau sab saum toj ntawm lwm cov khoom siv hluav taws xob ua kom sov, kom cov huab cua tuaj yeem ncig mus los.
Kev piav qhia ntawm Symbol siv

Ceev faj / Ceeb toom

DC, ncaj qha tam sim no

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

SAFETY THIAB WARRENTY
Guarantee thiab Liability
Cov xwm txheej dav dav ntawm kev muag thiab xa khoom ntawm Multi Channel Systems MCS GmbH ib txwm siv.
Lawv tuaj yeem pom hauv online ntawm http://www.multichannelsystems.com/sites/multichannelsystems.com/files/documents/Terms and Conditions.pdf Multi Channel Systems MCS GmbH ua rau tsis muaj kev lees paub txog qhov tseeb ntawm ib qho thiab tag nrho cov kev xeem thiab cov ntaub ntawv tsim los ntawm kev siv lub cuab yeej lossis software. Nws yog nyob ntawm tus neeg siv los siv qhov kev sim ua kom zoo los tsim kom muaj kev siv tau ntawm nws qhov kev tshawb pom.
Kev lees paub thiab kev lav phib xaub thaum muaj kev raug mob lossis cov khoom puas tsuaj raug cais tawm thaum lawv yog qhov tshwm sim ntawm ib qho ntawm cov hauv qab no:
· Kev siv tsis raug ntawm lub cuab yeej. · Kev teeb tsa tsis raug, kev ua haujlwm, kev ua haujlwm lossis kev saib xyuas ntawm lub cuab yeej. · Ua haujlwm lub cuab yeej thaum muaj kev nyab xeeb thiab cov khoom siv tiv thaiv tsis zoo thiab / lossis ua haujlwm tsis tau. · Tsis ua raws li cov lus qhia hauv phau ntawv qhia txog kev thauj, khaws cia, teeb tsa, commissioning, ua haujlwm
los yog kev saib xyuas ntawm lub cuab yeej.
· Tsis tso cai hloov cov qauv rau lub cuab yeej. · Kev hloov kho tsis tau tso cai rau lub kaw lus. · Kev saib xyuas tsis txaus ntawm cov khoom siv uas yuav tsum hnav. · Kev kho tsis raug thiab tsis raug cai. · Tsis tau tso cai qhib lub cuab yeej lossis nws cov khoom. · Cov xwm txheej raug kev puas tsuaj vim muaj kev cuam tshuam ntawm lub cev txawv teb chaws lossis kev ua ntawm Vajtswv.
Tus neeg ua haujlwm lub luag haujlwm
Tus neeg teb xov tooj yuav tsum tso cai rau tib neeg los ua haujlwm ntawm lub cuab yeej, leej twg
· paub txog kev nyab xeeb ntawm kev ua haujlwm thiab kev tiv thaiv kev sib tsoo thiab tau raug qhia kom siv cov cuab yeej siv; · muaj peev xwm tsim nyog lossis muaj kev paub tshwj xeeb thiab kev cob qhia thiab tau txais kev qhia txog kev siv cov cuab yeej; · tau nyeem thiab nkag siab txog tshooj ntawm kev nyab xeeb thiab cov lus ceeb toom hauv phau ntawv no thiab lees paub qhov no nrog lawv
kos npe.
Nws yuav tsum tau saib xyuas ntawm lub sijhawm tsis tu ncua uas cov neeg ua haujlwm ua haujlwm tau ua haujlwm zoo. Cov neeg ua haujlwm tseem tab tom kawm yuav tsuas ua haujlwm ntawm lub cuab yeej raws li kev saib xyuas ntawm tus neeg muaj kev paub dhau los.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

Taw qhia

INTRODUCTION Zoo siab txais tos rau CMOS-MEA5000-System
Multi Channel Systems txaus siab los nthuav qhia CMOS-MEA5000-System. Raws li kev siv thev naus laus zis hlau-oxide semiconductor, nws qhib qhov muaj peev xwm tshiab hauv kev tshawb fawb electrophysiological. Nrog ntau tshaj 4000 qhov chaw kaw, txhua tus ntawm lawv sampcoj mus txog 25 kHz, lub nti tso cai rau cov ntaub ntawv kaw tseg ntawm qhov siab heev spatio-temporal daws teeb meem. Los ntawm suav nrog amplification ntawm lub nti nws tus kheej, suab nrov yog txo qis thiab lub teeb liab zoo yog lav. Stimulation yog muab los ntawm 1024 qhov chaw stimulation suav nrog hauv nti thiab lub tshuab hluav taws xob stimulus hauv lub taub hautage. CMOS-MEA5000-System muaj peb yam, uas yog txhua tus tsim kom muaj txiaj ntsig zoo thiab muaj zog, thaum tuav tus hneev taw me me. CMOS-Chip Cov nti yog ua raws li cov khoom siv hlau oxide semiconductor (CMOS) ua ke, ua kom yooj yim ceev, siab daws teeb meem ntawm cov hluav taws xob ua haujlwm. Lub nti yog nruab nrog kab lis kev cai los yog hlais chamber rau koj lub tsevample, thaum tso cai siv lub tshuab tsom. taub hautage Lub hauv paus ntawm qhov system yog lub taub hautage. Nws samples cov ntaub ntawv los ntawm nti ntawm 25 kHz ib channel. Dhau li A/D conversion thiab amplification, lub taub hautage kuj muaj peb-channel stimulator. Koj tuaj yeem tsim cov qauv kev txhawb nqa ntawm software thiab xaiv txhua qhov ntawm 1024 qhov chaw txhawb nqa. Interface Board Lub interface board IFB-C muaj USB 3.0 interface los hloov cov ntaub ntawv kaw rau lub computer. Ntxiv mus, nws muaj analog thiab digital in- thiab outputs rau synchronization nrog rau lwm yam twj paj nruag. Txuas lub taub hautage ntawm iX cable rau lub interface board. Txuas lub interface board ntawm USB-C cable rau cov ntaub ntawv tau txais lub computer. Txuas lub interface board rau lub qhov hluav taws xob. Siv lub interface board yog tias tsim nyog. Thov siv lub qhov (socket) hauv av ntawm lub vaj huam sib luag tom qab ntawm IFB-C. Khoos phib tawj nrog Software Cov software CMOS-MEA-Control thiab CMOS-MEA-Tools yog programmed tshwj xeeb rau CMOS-MEA5000-System. Nws pab txhawb kev ua haujlwm ntawm lub sijhawmview ntawm lub nti ua tiav nrog lub peev xwm zoom thiab ntau yam cuab yeej los txheeb xyuas cov ntaub ntawv.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

HARDWARE

HARDWARE taub hautage
Muaj peb yam khoom siv kho vajtse rau CMOS-MEA5000-System muaj, lub taub hautage, lub interface board thiab cov ntaub ntawv tau txais lub computer. Yog tias tsim nyog, koj tuaj yeem siv lub tshuab ntsuas kub ntxiv. Rau kev teeb tsa thiab txuas CMOS-MEA5000-System, thov nyeem tshooj tom ntej "Hardware Setup" nyob rau nplooj 14. Cov taub hautagesamples cov ntaub ntawv los ntawm 4225 sensors ntawm lub nti ntawm 25 kHz ib channel. Dhau li A/D conversion thiab amplification, lub taub hautage kuj muaj peb-channel stimulator. Koj tuaj yeem tsim peb tus qauv stimulation ntawm software thiab xaiv txhua qhov ntawm 1024 stimulation sites. Hloov cov ntaub ntawv yog muab los ntawm iX cable los ntawm lub taub hautage mus rau lub interface board. Lub USB-C cable kev twb kev txuas npaj ceev ceev cov ntaub ntawv hloov los ntawm lub interface board mus rau lub computer tau txais cov ntaub ntawv. Txuas lub ntsuas kub TC rau lub taub hautage, yog tias tsim nyog. Ntxig cov qauv kev sojntsuam lossis CMOS-MEA-Chip hauv kev taw qhia kom raug rau hauv qhov chaw muab hauv lub taub hautage. Lub npoo npoo ntawm qhov kev sojntsuam lossis MEA yuav tsum nyob rau hauv pem hauv ntej ntawm sab laug, thaum saib ncaj qha mus rau lub taub hau qhibtage. Qhov no txoj kev CMOS-Control software qhia cov electrodes nyob rau hauv txhua kab thiab kab raws li qhia nyob rau hauv lub tswvyim hauv qab no. Electrode No 1 yog muab tso rau ntawm sab laug qis puag ncig ntawm lub nti thiab electrode No 4225 muab tso rau hauv sab xis sab xis.
Chip Orientation nyob rau hauv lub taub hautage Yog tias koj stimulating nrog lub teeb los yog ntxiv electrodes los ntawm sab nraum lub nti, los yog yog hais tias lub orientation ntawm cov ntaub so ntswg yog ib qho tseem ceeb, thov nco ntsoov tias txoj kab ntawm lub zeem muag yog los ntawm lub taub hau.tage sab qhov chaw txuas rau IFB nyob.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

HARDWARE
CMOS-MEA Chip

Lub nti yog ua raws li cov khoom siv hlau oxide semiconductor (CMOS), ua kom yooj yim, kev daws teeb meem ntawm hluav taws xob ua haujlwm. Lub nti yog nruab nrog lub chamber kab lis kev cai rau tsev koj sample, thaum tso cai siv lub tshuab tsom.
CMOS-MEA array yog ib qho khoom siv ua haujlwm, sib piv rau cov MEA passive. Nws yuav tsum tau muab lub zog nce thiab nqis kom zoo, lossis nws yuav puas.
Ceeb toom: Ua ntej qhib CMOS-MEA taub hautage rau tshem tawm CMOS-MEA nti, nws yog ib qho tsim nyog yuav tsum tau siv lub zog nti, txwv tsis pub lub nti yuav raug puas tsuaj!
CMOS sensor arrays yog lub teeb rhiab heev. Thaum kaw lub sensors yuav tsum tau lub teeb nyob tas li. Qhov no yog ib qho yooj yim kom tau txais los ntawm kev npog CMOS array nrog lub chamber tsaus "CMOS-DC".

CMOS-DC

CMOS-TH

Thov siv CMOS cov ntaub so ntswg tuav "CMOS-TH" kom khaws cov kab mob hnyav. Muaj rau CMOS-MEA nti SCG thiab CCM.

CMOS-MEA nti muaj 65 x 65 layout thiab muaj nyob nrog 16 m lossis 32 m interelectrode nrug (nruab nrab rau nruab nrab). Lub electrode txoj kab uas hla yog ib txwm 8 m. Nyob nruab nrab ntawm cov ntaubntawv povthawj siv electrodes, muaj ib daim phiaj ntawm 32 x 32 loj stimulation qhov chaw. Piv txwv li, koj tuaj yeem sau los ntawm 4225 electrodes thiab txhawb koj lub sample ntawm 1024 qhov chaw. Lub nti yog coated nrog ib tug planar oxide, zoo ib yam li iav, txhim khu kev qha
biocompatibility thiab biostability. Thov saib cov electron micrograph ntawm CMOS-MEA nti nto (NMI Reutlingen, Lub teb chaws Yelemees) thiab
lub schema ntawm lub nti hauv qab no.

Lub 16 m interelectrode nrug nti muab qhov kev daws teeb meem siab tshaj plaws. Nrog rau cov electrodes siab, koj tuaj yeem sau los ntawm qhov chaw loj (1 mm² @ 16 m nrug, 4 mm² @ 32 m nrug). Yog li ntawd, koj tuaj yeem pom cov cim qhia los ntawm txhua lub xov tooj ntawm tes thiab txawm tias lub teeb liab nthuav tawm raws li axon, thaum tseem tau txais kev tshaj tawm.view ntawm koj tiav sample.
Koj cov ntaub ntawv yog sampcoj mus txog 25 kHz ib channel. Yog li, tsis muaj teeb liab ploj - txawm tias axonal spikes tau tshwm sim thiab kaw kom huv si. Ua ke nrog kev hloov pauv A / D ntawm 14 ntsis, lub kaw lus ua kom cov ntaub ntawv raug thiab meej.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

HARDWARE
Tam sim no ob hom kab lis kev cai muaj rau CMOS-MEAs: Ib qho rau cov kab lis kev cai ntawm tes thiab ib qho rau cov kab mob hnyav. Hauv ob hom chips ib qho av electrode twb tau ua ke.
CCM Culture chamber rau cell cultures nrog MEA-MEM hau.
SCG Slice Chav. CMOS-MEA nti nrog SCG Slice Chamber nrog cov txheej txheem siab tshaj plaws rau laminar ntws. Lub warranty ntawm CMOS-MEA nti yog rau lub hlis txij li hnub xa khoom.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

HARDWARE
Interface Board IFB-C
Lub multiboot interface board IFB-C txhawb kev ua haujlwm ntawm txhua MCS hauv vitro thiab hauv vivo hautages nyob rau hauv tag nrho 2100 amplifier daws suite. Cov suite no suav nrog: MEA2100-HS, Multiwell-MEA-HS, CMOS-MEA-HS, MEA2100-Beta-Screen-HS, W2100-HS thiab ME2100-HS. Lub modular 2100 amplifier tov suite tsim ua rau nws yooj yim los hloov koj cov khoom siv lab feem ntau nrog cov cuab yeej kho vajtse kho kom zoo dua qub. Pem hauv ntej vaj huam sib luag
SYNC Tawm / Hauv Ob lossis ntau lub rooj sib tham IFB-C tuaj yeem daisy-chained los ntawm kev siv SYNC Tawm / SYNC Hauv cov khoom sib txuas. Tag nrho cov daisy-chained interface boards khiav ntawm tib lub moos kom tso cai rau cov ntaub ntawv kaw cia zoo kawg nkaus hauv cov chaw loj. Analog Channels txog li yim Analog Hauv cov channel muaj nyob ntawm 10-pin connector. Thov nyeem tshooj 10-Pin Connector rau Analog IN hauv Cov Ntawv Ntxiv rau cov lus qhia ntxiv txog tus pin layout. Cov analog inputs ntxiv yog npaj rau sau cov ntaub ntawv ntxiv los ntawm cov khoom siv sab nraud, rau example, rau sau thaj clamp nyob rau hauv parallel rau MEA cov ntaub ntawv. Analog Channels 1 thiab 2 Ob ntawm cov yim analog channels (Analog Hauv No 1 thiab No 2) yog cais muaj nyob ntawm Lemo connectors nyob rau pem hauv ntej vaj huam sib luag ntawm lub interface board IFB-C. Ob Qhov Status LEDs Cov xwm txheej LEDs qhia txog qhov txuas ntawm HS 1 thiab / lossis HS 2. Lawv teeb thaum ib lossis ob lub taub hautages txuas nrog IFB-C interface board ntawm iX-kev lag luam cable.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

HARDWARE
Digital IN / OUT A Digital IN / OUT rau 16 cov khoom siv hauv- thiab cov khoom tso tawm yog muaj (Honda-PCS-XE68LFD) ntawm lub vaj huam sib luag tom qab ntawm lub rooj sib tham. Nyob rau pem hauv ntej vaj huam sib luag plaub Digital IN thiab plaub Digital OUT cov khoom kuj tseem siv tau los ntawm Lemo connector (DIG IN ntsis 0 txog ntsis 3 thiab DIG OUT ntsis 0 txog ntsis 3). Lub Digital OUT xa TTL pulses nrog 3.3 V lossis 5 V. Lub voltage tuaj yeem hloov pauv ntawm ob lub voltages nrog software IFB-Control, thov nyeem tshooj “IFB-Control” hauv Cov Ntawv Ntxiv.

Yog tias nkag mus rau ntau qhov ntawm DIG IN / OUT channel yog xav tau, nws yuav tsum tau txuas rau Digital IN / OUT txuas ntxiv Di / o board nrog 68-pin standard cable. Lub Rooj Tswjhwm Saib Di/o no muaj raws li kev xaiv khoom siv. Hauv av Yog xav tau kev sib txuas hauv av ntxiv, koj tuaj yeem txuas lub ntsaws no nrog lub hauv av sab nraud siv tus qauv sib txuas (4 mm).
Lub Vaj Huam Sib Luag
Toggle Hloov On / Off Toggle hloov rau tig lub cuab yeej rau thiab tawm. CMOS-MEA5000-System tau hloov mus rau qhov xwm txheej "ON" thaum hloov pauv hloov mus rau sab laug. Cov cuab yeej tau hloov "OFF" thaum lub toggle hloov mus rau sab xis. Yog tias lub kaw lus "ON", thiab cov cuab yeej txuas nrog rau txoj kab hluav taws xob, Lub Hwj Chim LED ntawm lub vaj huam sib luag pem hauv ntej ntawm lub rooj sib tham yuav tsum teeb. Yog tias tsis yog, thov xyuas qhov chaw siv hluav taws xob thiab cable. Fais fab Txuas lub hwj huam mov ntawm no. Cov khoom siv hluav taws xob no ua rau ob qho tib si, lub taub hautage thiab interface board ntawm CMOS-MEA5000System. Cov cuab yeej xav tau 24 V thiab 2.5 A / 65 W. Hauv av Yog xav tau kev sib txuas hauv av ntxiv, koj tuaj yeem txuas lub ntsaws no nrog rau hauv av sab nraud siv tus qauv jack (4 mm).

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

HARDWARE
Digital IN / OUT
A Digital IN / OUT rau 16 digital in- thiab output bits yog muaj nyob ntawm Honda-PCS-XE68LFD connector. Thov nyeem tshooj Digital IN / OUT Connector hauv Cov Ntawv Ntxiv rau cov lus qhia ntxiv txog tus pin layout ntawm lub connector. Kev sib txuas Digital IN / OUT lees txais lossis tsim cov qauv TTL cov cim. Lub Digital OUT xa TTL pulses nrog 3.3 V lossis 5 V. Lub voltage tuaj yeem hloov pauv ntawm ob theem no nrog software IFB-Control, thov nyeem tshooj “IFB-Control” hauv Cov Ntawv Ntxiv.
TTL sawv cev rau Transistor-Transistor Logic. Lub TTL mem tes txhais tau tias yog lub teeb liab digital rau kev sib txuas lus ntawm ob lub cuab yeej. Ib voltage ntawm 0 V thiab 0.8 V yog suav hais tias yog lub xeev cov ntsiab lus ntawm 0 (LOW), thiab voltage ntawm 2 V thiab 3.3 V lossis 5 V txhais tau tias 1 (HIGH).
Lub Digital OUT tso cai tsim cov teeb liab digital nrog txog 16 khoom thiab nyeem nws tawm, piv txwv liample, los ntawm kev siv Digital IN / OUT txuas ntxiv Di/o board. Koj tuaj yeem siv cov teeb liab digital no los tswj thiab synchronize lwm cov khoom siv nrog MEA2100-Beta-Screen-System.
Ntsis 0 mus rau 3 ntawm Digital OUT yog sib cais thiab muaj raws li Lemo connector DIG OUT 0 mus rau 3 nyob rau pem hauv ntej vaj huam sib luag ntawm lub interface board. Yog li, qhov kev txuas ntxiv Di/o tsuas yog tsim nyog yog tias xav tau ntau tshaj plaub qhov kev tawm tswv yim lossis cov khoom tawm.
Lub Digital IN tuaj yeem siv los sau cov ntaub ntawv ntxiv los ntawm cov khoom siv sab nraud ua tus lej 16 me ntsis. Lub Digital IN feem ntau yog siv los ua cov kaw lus nrog TTL teeb liab. 16-ntsis digital input channels yog kwj ntawm 16 ntsis qhov tseem ceeb. Lub xeev ntawm txhua qhov me me (0 txog 15) tuaj yeem tswj tau nyias. Tus qauv TTL cov teeb liab raug lees txais raws li cov teeb liab tawm tswv yim ntawm cov khoom siv digital.
Warning: A voltage uas siab dua +3.3V lossis +5V lossis qis dua 0V, uas yog, qhov tsis zoo voltstage, siv rau cov tswv yim digital yuav rhuav tshem cov khoom siv hluav taws xob. Nco ntsoov tias koj tsuas siv TTL pulses (0 txog 3.3 V lossis 5 V) rau cov khoom siv digital.
Pab Txhawb Channel
Ob txoj kev pabcuam tshwj xeeb muaj rau kev siv yav tom ntej. Lawv tsis muaj haujlwm tam sim no.
Suab Tawm
Qhov "Audio OUT" muaj nuj nqi tsis muaj rau CMOS-MEA5000-System.
DSP JTAG Txuas
Tus J.TAG connector yog siv los program lub teeb liab processor DSP rau real-time feature. Cov yam ntxwv no tsis siv hauv CMOS-MEA5000Systems.
HS / SCU
Qhov (sockets) txuas mus txog ob lub CMOS-MEA-System taub hautages ntawm iX-industrial cable(s), hom B.
USB-C Connectors B thiab A
Ob lub USB-C connectors yog siv los hloov cov amplified thiab digitized cov ntaub ntawv los ntawm tag nrho cov ntaub ntawv raws thiab ntxiv cov digital thiab analog channel rau cov ntaub ntawv txuas nrog lub khoos phis tawj ntawm USB-C cable. Connector A sib txuas nrog iX input 1, thiab connector B nrog iX input 2. Yog siv ob qho iX inputs, kuj yuav tsum tau siv ob qho tib si USB-C kev sib txuas. Ob lub USB cables yuav tsum tau txuas nrog sib txawv USB-C ports ntawm lub computer, tsis txhob siv lub hub USB! Tsuas yog siv cov qib siab USB-C cables, raws li tau muab nrog lub kaw lus.
Tseem ceeb: Nws raug pom zoo kom txuas USB-C cable ncaj qha mus rau USB 3.0 chaw nres nkoj ntawm lub computer. Tsis txhob siv USB hub!
Data Acquisition Computer
Lub software CMOS-MEA-Control tau programmed tshwj xeeb rau CMOS-MEA5000-System. Nws pab txhawb kev ua haujlwm ntawm lub sijhawmview ntawm lub nti ua tiav nrog lub peev xwm zoom thiab ntau yam cuab yeej los txheeb xyuas cov ntaub ntawv. Thov nyeem tshooj “CMOS-MEA-Control” kom paub txog cov software.
Lub khoos phis tawj tau txais cov ntaub ntawv yog muab los ntawm Multi Channel Systems MCS GmbH. Qhov kev ua haujlwm ntawm Windows ® 10 lossis 8.1 yog qhov tsim nyog.
Vim muaj cov ntaub ntawv sau tseg loj heev, lub khoos phis tawj uas muaj kev ua haujlwm tsawg yuav ua rau muaj teeb meem kev ua haujlwm; Yog li ntawd, Multi Channel Systems muab lub khoos phis tawj tshiab nrog USB 3.0 kev sib txuas thiab Intel nti teeb. Siv lub hard drive SSP rau cov ntaubntawv povthawj siv thiab lub hard drive thib ob rau thaub qab. Hauv daim ntawv qhia sampling nrog siab tshaj sampling tus nqi tam sim no siv 1TB SSD tsav yuav tuav txog ib teev ntawm kev kaw cia tas li nkaus xwb!

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

HARDWARE teeb tsa
HARDWARE SETUP Thov ua raws li cov lus qhia: 1. Txuas lub CMOS-MEA5000 amplifier ntawm iX-kev lag luam cable, hom B mus rau IFB-C interface board. Thov siv lub plug nyob rau hauv daim ntawv lo
nrog "1" nyob rau sab nraum qab ntawm lub interface board. 2. Txuas lub IFB-C ntawm lub hwj chim unit mus rau lub qhov hluav taws xob. 3. Txuas IFB-C ntawm USB-C rau lub computer. Siv lub USB-C plug nyob rau hauv daim ntawv lo "A" nyob rau sab nraum qab ntawm lub interface board.
4. Txuas IFB-C ntawm USB-C cable mus rau sab nraub qaum ntawm lub computer. Nws yog qhov yuav tsum tau siv qhov chaw nres nkoj USB 3.0, thov saib daim duab hauv qab no!
Tseem ceeb: Nws yog ib qho tsim nyog los txuas lub rooj sib tham IFB-C ntawm CMOS-MEA5000-System rau Intel ® USB 3.0 chaw nres nkoj. Txwv tsis pub, koj pheej hmoo cov ntaub ntawv poob.
Thov xav txog cov lus yuam kev thaum pib CMOS-MEA-Control software thawj zaug. Tseem ceeb: Nco ntsoov tias txhua txoj hauv kev rau kev kaw files mus rau SSD tsav txwv tsis pub kaw tsis tau! 5. Txuas lub ntsuas kub ntawm TC ntawm USB 2.0 kev kub ceev cable mus rau ib qho ntawm USB 2.0 chaw nres nkoj thiab ntawm chav tsev fais fab mus rau
lub qhov hluav taws xob. 6. Txuas cov khoom cua sov ntawm CMOS hautage ntawm muab cable rau lub ntsuas kub.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

HARDWARE teeb tsa
Installation ntawm Software
Cov Kev Xav Tau
Yog tias koj tau yuav CMOS-MEA5000-System nrog PC txhua yam yog preinstalled thiab sim. Yog tias koj yuav ib lub kaw lus tsis muaj PC thov nco ntsoov tias lub khoos phis tawj ua tau raws li peb cov kev qhia tshwj xeeb ntsig txog processor, nco, hard disk, thiab lwm yam. Thov hu rau Multi Channel Systems MCS GmbH lossis koj lub khw muag khoom hauv zos.
Software: Ib qho ntawm cov hauv qab no Microsoft Windows ® operating systems yuav tsum tau: Windows 10 lossis 8.1, 64 ntsis (Lus Askiv thiab German versions txhawb) nrog NT file qhov system. Lwm hom lus versions yuav ua rau software yuam kev.
Vim muaj cov ntaub ntawv kaw tseg, lub khoos phis tawj uas muaj kev ua haujlwm tsawg yuav ua rau muaj teeb meem kev ua haujlwm; Yog li ntawd, Multi Channel Systems MCS GmbH pom zoo kom muaj lub khoos phis tawj tshiab. Thov hu rau MCS los yog koj lub khw muag khoom hauv zos kom paub ntau ntxiv txog kev pom zoo cov khoom siv kho vajtse.
Qhov tseem ceeb: Vim tias cov ntaub ntawv tau txais ntau heev (txog 220 MByte ib ob), nco ntsoov tias txhua txoj hauv kev rau kev kaw files mus rau SSD tsav, txwv tsis pub kaw tsis tau!
Thov nco ntsoov tias qee zaum muaj cov khoom siv tsis sib haum xeeb ntawm cov ntaub ntawv tau txais thiab cov khoom siv hauv computer; los yog hais tias lub tshuab hluav taws xob tsis tsim nyog yuav ua rau cov teeb meem tsis raug.
Pom zoo Operating System Settings
Cov kev pabcuam tsis siv neeg hauv qab no ntawm Windows operating system cuam tshuam nrog cov ntaub ntawv khaws cia ntawm lub hard disk thiab tuaj yeem ua rau muaj kev txwv tsis zoo hauv CMOS-MEA-Control. Cov kev niaj hnub no tau tsim los siv rau hauv chaw ua haujlwm khoos phis tawj, tab sis tsis muaj txiaj ntsig zoo rau lub khoos phis tawj tau txais cov ntaub ntawv.
· Deselect "Windows Indexing Service" rau cov ntaub ntawv SSD thiab HD disks, qhov system hard drive tsis suav nrog. · Hloov tawm hom pw tsaug zog rau cov lus qhia thiab HD disks. · Kev xaiv lub hwj chim: Lub tswv yim fais fab: kev ua haujlwm siab. Tsis txhob qhib qhov system standby. · Tig tawm "Optimize hard disk thaum tsis ua haujlwm", qhov tsis siv neeg disk fragmentation. · Tua lub Screen Saver thiab tsis txhob siv tus kab mob scanner thaum sim. · Nws kuj tsis pom zoo kom khiav ib daim ntawv thov hauv keeb kwm yav dhau thaum siv CMOS-MEA-Control. Tshem tawm txhua daim ntawv thov
los ntawm "Autostart" folder.
Tseem ceeb: Thov nco ntsoov kom muaj kev tswj hwm koj lub computer ua tus thawj coj. Txwv tsis pub, nws muaj peev xwm hais tias lub teeb tsa software tsis ua haujlwm zoo.
Doubleclick lub CMOS-MEA-Control.exe ntawm lub ntim ntim. Tus pab cuam installation yuav tshwm sim thiab coj koj mus rau txoj kev teeb tsa. Ua raws li cov lus qhia ntawm tus pab cuam installation.
Nco tseg: Thaum lub sijhawm teeb tsa, tag nrho cov tsav tsheb raug teeb tsa thiab yog tias tsim nyog, lub firmware ntawm CMOS-MEA5000 kho vajtse tau hloov kho. Thov tsis txhob cuam tshuam cov firmware hloov tshiab.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

TEST CMOS-MEA5000-SYSTEM
Kev Ntsuas CMOS-MEA5000-System Functional Tests ntawm CMOS-MEA5000-System nrog Kev Ntsuas Tus Qauv Ntsuas
Thov nyeem cov ntaub ntawv "Test-CMOS-MEA" hauv Cov Ntawv Ntxiv. Ntxig "Test Model Probe" hauv kev taw qhia kom raug thiab kaw lub taub hautage. Lub puag ncig ntawm Test-CMOS-MEA lossis CMOS-MEA nti yuav tsum nyob rau hauv pem hauv ntej ntawm sab laug thaum saib ncaj qha rau qhib amplifier. Cov qauv muab kev sojntsuam simulates CMOS-MEA nti nrog cov resistor ntawm 100 k thiab 10 p capacitor ntawm hauv av thiab txhua kab ntawm 65 x 65 electrodes nyob rau hauv daim phiaj. Nws tuaj yeem siv rau kev ntsuas lub suab nrov ntawm CMOS-MEA5000-System, rau kev sim ntsuas ntsuas thiab ntsuas cov stimulators sab hauv. CMOS-MEA xeem qauv soj ntsuam thiab CMOS-MEA chips yog cov khoom siv nquag thiab yuav tsum tau hloov thiab tua kom raug, txwv tsis pub lawv yuav raug kev puas tsuaj.
Thov nyem lub MEA array icon nyob rau hauv "Cov ntaub ntawv Source" qhov rai. CMOS chips yuav tsum tau ntsuas ua ntej txhua qhov kev siv. Lub calibration khiav tau thiab feem ntau yuav siv sij hawm ob mus rau peb feeb. Thov tsis txhob cuam tshuam cov txheej txheem, kom txog rau thaum cov lus kawg "Finished automatic system calibration" tshwm. Qhov "Test CMOS-MEA" simulates lub calibration ntawm lub nti. Lub ,,ODD" cable txhawb nqa cov lej khib thiab cov ,,EVEN" cable txhawb nqa cov lej txawm tias. Rau lub hom phiaj los xyuas cov suab nrov ntawm CMOS-MEA5000-System yam tsis muaj cov teeb liab sab nraud, thov txuas lub cable soldered mus rau ,,ODD "connector rau cov khoom siv hluav taws xob ntawm cov xov tooj sib txuas thiab cov cable soldered rau ,,EVEN "Connector rau ,, BATH" input. Txuas nyob rau hauv txoj kev no, lub teeb liab tawm tswv yim txhawb nqa tag nrho 65 kab ntawm 65 x 65 layout ntawm CMOS-MEA nti ntawm ib lub sij hawm thiab simulates kuj calibration ntawm da dej. Disconnect tus "ODD" connector kom pom cov "EVEN" tooj electrodes nkaus xwb thiab vice versa pom cov "ODD" tooj electrodes xwb.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

TEST CMOS-MEA5000-SYSTEM
Kev ntsuam xyuas ntawm Internal Stimulators Test-CMOS-MEA sojntsuam yog nruab nrog peb qhov txuas ntxiv los kuaj qhov kev txhawb siab sab hauv, ,,STG1″, ,,STG2″ thiab ,,STG3″. Cov stimuli yog xim coded nyob rau hauv CMOS-MEA-Control software: Stimulus 1 yog qhia nyob rau hauv ntsuab xim, Stimulus 2 yog qhia nyob rau hauv xiav thiab Stimulus 3 nyob rau hauv cov xim liab.
Txhawm rau kuaj lub stimulator, thov txuas lub cable ntawm "ODD" txuas rau "EVEN" txuas thiab qhib cable rau ib qho ntawm "STG" ntsaws. Txhais tus qauv stimulus nyob rau hauv lub qhov rais "Stimulation", piv txwv liample aramp nrog 100 ms ntawm stimulator 1 (ntsuab), raws li qhia nyob rau hauv lub screenshot saum toj no. CMOS-MEA Chip
Thov sau CMOS-MEA nti nrog PBS. CMOS sensors yog lub teeb rhiab heev. Txhawm rau sau cov hauv paus ruaj khov CMOS-MEA array yuav tsum muaj nyob rau hauv lub teeb ruaj khov, uas tuaj yeem muab tau yooj yim los ntawm kev npog CMOS-MEA nrog lub chamber yooj yim tsaus "CMOS-DC". Txwv tsis pub, lub teeb liab yuav drift tawm ntawm ntau yam los ntawm cov nyhuv ntawm lub teeb.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

GENERAL SOFTWARE NTA

General Software Features Tshooj "General Software Features" piav txog qee qhov ntawm CMOS-MEA-Control thiab CMOS-MEA-Tools software nta ntawm lub hauv paus ntawm examples. Numeric Up-down Box
Kho tus nqi nyob rau hauv tus lej nce-down lub npov los ntawm txhaj rau lub xub khawm lossis los ntawm txhaj rau hauv lub qhov rais thiab txav lub log nas. Tig lub log rau pem hauv ntej thiab qib nce, tig lub log rov qab thiab tus nqi txo qis hauv cov kauj ruam nrawm. Siv cov nyees khawm xub txhawm rau kho qhov kev hloov kho kom zoo. Qhov thib peb muaj peev xwm yog hloov ntawm tus lej hauv lub qhov rais los ntawm kev sau nws, yog tias tus nqi tau txhais ua ntej, rau example. Zoom In thiab Zoom Out Zoom Buttons
Nyem qhov "Kho kom teeb liab Min/Max" khawm. Qhov scaling ntawm y-axis yog teem rau qhov tsawg kawg nkaus thiab siab tshaj plaws ntawm tag nrho cov pom sampnyob rau hauv lub channel.
Nyem "Zoom" khawm. Zooming nyob rau hauv txiav qhov scaling ntawm lub axis nyob rau hauv ib nrab thiab zooming tawm doubles lub scaling.
Zoom los ntawm nas-nias Ntxiv mus, rau lub zoom nyees khawm koj tuaj yeem ua kom zoom mus rau hauv ib cheeb tsam ntawm kev txaus siab los ntawm kev txav tus nas hauv ib qho screen ntawm sab laug mus rau sab xis thaum nias lub pob laug nas. Txav tus nas los ntawm sab xis mus rau sab laug kom zooming tawm.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

GENERAL SOFTWARE NTA
Zaub Floating
Tsim koj tus kheej cov zaub nrog lub floating feature. Decouple ib lub dialog zaub ntawm koj xaiv thiab tso nws nyob qhov twg koj xav tau.
Dock lub qhov rais dua los ntawm txhaj nrog txoj cai nas khawm nyob rau sab saum toj ntawm lub qhov rais. Nkaum lub qhov rais nrog qhov "Auto-Hide" kev xaiv. Tsim cov cheeb tsam ntawm kev txaus siab ROIs
Xaiv ROIs manually los ntawm kos duab plaub yog zoo tib yam txawm nyob rau hauv "Kev Ua Haujlwm" lossis "Sensor Array Tool" qhov rai. Thov cia tus nas laug khawm nias kom kos ib daim duab plaub hauv "Kev Ua Haujlwm" qhov rai los tsim ib cheeb tsam txaus siab. Ib kab dashed hauv xiav qhia txog ciam teb ntawm ROI. Cov xim ntawm cov duab plaub tig mus rau xim dub thiab ID ntawm ROI tshwm nyob rau sab xis sab xis. Los yog tsim ROI los ntawm txhaj rau ib qho ntawm cov haujlwm maxima. Hloov kho thaj tsam ntawm kev txaus siab yog nyem rau ntawm ciam teb kom txog rau thaum ob lub xub tshwm sim txav ciam teb. Tshem tawm ROI los ntawm txhaj rau ROI dua. Lub sensor channels suav nrog hauv thaj tsam ntawm kev txaus siab tau pom tam sim ntawd hauv "ROI" qhov rai ntawm ib sab.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-CONTROL SOFTWARE

CMOS-MEA-CONTROL Introduction Lub software rau tswj CMOS-MEA5000-System suav nrog ob ntu, CMOS-MEA-Control rau kev kaw hauv online, CMOS-MEA-Cov cuab yeej rau kev tshuaj xyuas offline. CMOS-MEA-Control software yog tsim los rau kev sau cov ntaub ntawv online nrog CMOS-MEA5000-System. Nws pab txhawb kev ua haujlwm ntawm lub sijhawmview ntawm lub nti ua tiav nrog lub peev xwm zoom rau hauv cov ntaub ntawv nyoos thiab ntau yam cuab yeej los pom kev ua haujlwm. CMOS-MEA-Control software tswj CMOS-MEA5000-System, kev sim cov txheej txheem, xaiv cov ntaub ntawv rau cov ntaub ntawv kaw rau hard disk thiab cov haujlwm zoo sib xws xws li kev tswj kev txhawb nqa, kev tshawb nrhiav hauv online, kev txuag ntawm spikes nrog lossis tsis muaj cov ntaub ntawv raw. kom txuag tau qhov chaw disk lossis xaiv thaj tsam ntawm kev txaus siab xaiv rau cov ntaub ntawv txo thiab ntau yam ntxiv.
Qhov rai Main

Lub qhov rais default ntawm cov ntawv qhia zaub mov tseem ceeb tau muab faib ua peb ntu sib txuas uas yog framed los ntawm cov ntawv qhia zaub mov saum toj no:
1. Sab laug: Cov seem tswj los tswj CMOS-MEA5000-System hardware, cov ntaub ntawv tau txais, cov txheej txheem kev sim, kev kaw cov ntaub ntawv, cov streaming ntawm kuaj pom spikes thiab ib ntu los saib xyuas lub kaw lus load.
2. Nruab nrab: Cov cuab yeej ntu nrog cov cuab yeej los teeb tsa cov khoom siv sensor, saib xyuas cov ntaub ntawv tau txais hauv online, txhawm rau txheeb xyuas cov spikes, txhawb nqa thiab rho tawm cov xwm txheej los ntawm cov teeb liab tawm tswv yim.
3. Txoj cai: Cov ntaub ntawv view ntu nrog cov ncauj lus kom ntxaws views ntawm cov ntaub ntawv nyoos los yog ntawm cov kab nrib pleb. Koj tuaj yeem zoom rau hauv cov ntaub ntawv hauv thaj tsam ntawm kev txaus siab saum toj no thiab hauv cov ncauj lus kom ntxaws view ntawm ib tug kaw electrode hauv qab no.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-CONTROL SOFTWARE
Menu Bar File
Ntawv qhia zaub mov qhib thiab txuag templates thiab mus rau "Tawm" qhov kev pab cuam. Chaw
Cov ntawv qhia zaub mov kom qhib "Tools", "Activity Tool" thiab "Spike Tool". Hloov cov cuab yeej rau ntawm lub thawv kos kom ua lawv cov dej num los yog tawm los txo qhov system load. Thaum lub "Kev Ua Haujlwm Tool" raug kaw, tsis muaj cov ntaub ntawv nyoos yuav tshwm sim, tab sis tau ua tiav thiab khaws cia. Thaum lub "Spike Tool" raug rho tawm, tsis muaj kev kuaj pom kab mob tshwm sim, thiab tsis muaj pob txha raug khaws cia. Nco ntsoov, tias qhov kev kuaj pom ntawm ntau lub sensors yog ib txoj haujlwm kim thiab xav tau lub khoos phis tawj tsim nyog. Cov ntawv qhia zaub mov los teeb "Default Paths", rau "Txuag li Defaults" thiab "Reset Defaults". Nyem "Application Settings" qhib lub "Application Settings" dialog. Txhais txoj hauv kev rau "Default Paths" rau "Raw Data", "Template" thiab "Stimulation Files" hauv "Application Settings" dialog.

Tseem ceeb: Nco ntsoov tias txhua txoj hauv kev mus rau SSD tsav txwv tsis pub kaw tsis tau!
Nyem rau lub thawv "Txuag Sensor Calibration" hauv "Diagnostics" seem.
Nyem rau ntawm lub npov "Txuag Sensor Calibration" hauv "Diagnostics" seem kom txuag tau cov ntaub ntawv calibration rau "hdf5" file. Txhua lub sij hawm ua calibration, a file nrog calibration cov ntaub ntawv yog tsim nyob rau hauv lub "Raw Data" nplaub tshev, muaj cov ntaub ntawv raw thiab cov conversion yam xam los ntawm cov ntaub ntawv no.
Thaum lub sij hawm calibration txheej txheem, sensors yog calibrated ib yam nkaus. Rau qhov calibration voltage yog siv rau chav da dej. Cov teeb liab ntsuas ntawm cov sensors yog haum rau calibration stimulus. Yog tias ob qho tib si teeb liab haum zoo, qhov hloov pauv tau suav nrog, thiab ADC qhov tseem ceeb tau txais kev cawmdim hauv cov ntaub ntawv nyoos file.
Cov file cov npe pib nrog "Calibration" ua raws li hnub kaw thiab qhov txuas ntxiv: Calibration-2019.08.01-13.44.30.cmcr. Cov file hom ntawv yog cov ntaub ntawv raw file hom ntawv kom koj tuaj yeem thauj khoom rau kev tshuaj xyuas rau hauv "CMOS-MEA-Tools" software.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-CONTROL SOFTWARE
Lab Book
Thov siv cov ntawv nkag hauv peb lub tab ntawm phau ntawv lab rau kev tshuaj xyuas tom qab ntawm qhov kev sim. Nias lub pob "Teem Default" kom khaws cov ntaub ntawv raws li template. Tag nrho cov ntawv yuav raug cawm hauv cov ntaub ntawv nyoos file. Teeb tsa
Nyem "Device" hloov lub cuab yeej txuas. Yog tias kho vajtse muaj, xaiv "MCS Device". Yog tias tsis muaj khoom siv kho vajtse, thov siv "Simulator" lossis thauj khoom "Cov ntaub ntawv File"kom sim cov software yam tsis muaj kho vajtse txuas. Thaum replaying cov ntaub ntawv file, koj muaj lub advantage kom pom cov ntaub ntawv "tiag tiag" lub sijhawm no cov ntaub ntawv ntawm "Simulator" yog virtual.
Xaiv ib cov ntaub ntawv file los ntawm ib daim nplaub tshev, thov xaiv "Cov ntaub ntawv File"nyob rau hauv "Setup" menu. Lub qhov rais "Data Source" qhia "Simulation" ib sab ntawm CMOS-MEA5000-System khawm. Tom qab ntawd nyem rau ntawm CMOS-MEA nti khawm kom xaiv cov file koj xav replay los ntawm qhov browser dialog. Nyem "CMOS System" los qhib "Setup CMOS System" dialog.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-CONTROL SOFTWARE
"Setup CMOS System" dialog muab phau ntawv tswj rau qhov offset thiab calibration chaw rau CMOS nti. Muaj ib qho kev ua haujlwm tsis siv neeg rau lub nti pib thiab calibration, phau ntawv qhia feem ntau tsis xav tau. Thov nyeem tshooj “Kev Ua Haujlwm CMOS-MEAControl Software” kom paub meej cov ntaub ntawv. Qhib lub "Device Filter" dialog los hloov cov chaw lim.
Kho qhov teeb tsa ntawm "High Pass" thiab "Low Pass" lim ntawm qib kho vajtse thaum lub software tab tom khiav, tab sis tsis kaw. Xaiv "Bessel" lossis "Butterworth" lim "Tsev Neeg" hauv 1 lossis 2 "Kev Txiav Txim" los ntawm cov ntawv qhia zaub mov. Hloov "Tawm" qhov "Cutoff Frequency" ntawm lub siab dhau lim ua rau DC cov teeb liab. Yog tias cov chaw lim dej yuav tsum tau khaws cia rau kev sim tom qab, thov nyem lub pob "Set Permanent". Yog tias xav tau, thov teeb tsa "High Pass" lim rau kev xaiv "Tawm", uas txhais tau tias rau DC, ncaj qha voltage. Tsis muaj offset yuav raug siv, tag nrho cov ntaub ntawv nyoos raug tso tawm tsis muaj lim. Pab
Cov ntawv qhia zaub mov "Pab" yog qhib rau kev pab online thiab mus rau "Kuaj rau Hloov Kho", yog tias tsim nyog. Nyem rau "Txog" kev xaiv rau software thiab firmware cov ntaub ntawv. Nyem "Kev Pab Hauv Online" kom pom qhov kawg version ntawm CMOS-MEA5000-System phau ntawv. Nyem "Xaiv qhov hloov tshiab" txhawm rau saib seb lub software puas hloov tshiab, lossis ib qho tshiab muaj nyob rau ntawm Multi Channel Systems webqhov chaw.
Thaum pib CMOS-MEA-Control software no pop-up "Software Update Available" tshwm, thaum muaj cov software tshiab. Nyem rau ntawm daim ntawv txhawm rau qhib "Xaiv rau Kev Hloov Kho Tshiab" dialog kom muaj kev nkag ncaj qha thiab nrawm rau MCS web qhov chaw.

Yog hais tias lub software tsis hloov tshiab, nyem lub pob "Mus saib Web site" thiab rub tawm cov software tshiab tshaj plaws.

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CMOS-MEA-CONTROL SOFTWARE
Nyem qhov kev xaiv "Txog".
Thov saib cov kev qhia tshwj xeeb ntawm lub cuab yeej thiab tus lej version ntawm CMOS-MEA-Control software. Tswj Ntu "Control Section" muaj peb seem, "Cov ntaub ntawv Source", "Sau" thiab "Load" tswj. Cov ntaub ntawv Source

Lub qhov rais "Data Source" tso cai tswj cov ntaub ntawv qhov chaw thiab CMOS nti. Lub nti yog ib qho khoom siv nquag thiab yuav tsum tau siv zog ua ntej koj tuaj yeem pib qhov kev sim nrog "Start" khawm.

Nyem rau "Settings" icon

rau lub "Set Device" dialog. Thaum siv CMOS-MEA5000-System thawj zaug, thov txhais qhov twg

CMOS-MEA "Device" tam sim no siv thiab txhais cov "Sample Rate" los ntawm cov ntawv qhia zaub mov. Xaiv "Analog Channels" thiab cov

digital "Digital Channel" tso cai rau cov channel xaiv. Txiav txim siab nyob rau hauv "Cov Ntaub Ntawv" qhov rai seb koj puas xav kaw los ntawm cov channel no

los tsis yog, thov saib lub screenshot tom ntej.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-CONTROL SOFTWARE
Xaiv qhov siab "Sample Rate" ntawm 50 kHz, 100 kHz lossis 200 kHz, koj yuav tsum lees txais cov txiaj ntsig hauv qab no: Thov xaiv ib cheeb tsam ntawm kev txaus siab ROI nkaus xwb, kom tso cai rau cov ntaub ntawv kaw tseg ntawm cov ntaub ntawv loj hauv cheeb tsam ntawm cov khoom siv hluav taws xob. Lub qhov rais me me ntawm ib sab ntawm "Sample Rate" drop-down menu qhia txog qhov xaiv ROI ntxiv.

Lub kaw lus

Tswj qhov "Recorder" parameter nqis hauv qhov rais no. Pib thiab nres lub recorder thiab saib cov hom ntaub ntawv muaj. Nias lub

"Settings" icon

hloov chaw recorder. Thov nyeem tshooj “Kev Ua Haujlwm CMOS-MEA-Control” kom paub meej cov ntaub ntawv hais txog

programming lub recorder.

Pib thiab nres cov ntaubntawv povthawj siv manually nrog lub "ON / OFF" icon.

Thaum lub recorder khiav, lub sij hawm kaw thiab txoj kev uas yuav khaws cov ntaub ntawv file yog tso tawm. Txhais cov ntaub ntawv no txoj hauv kev "Recorder Settings" dialog. Definition ntxiv cov "File Lub npe" thiab "Prefix" thiab "Suffix".

Pib thiab nres lub kaw lus nyob rau hauv peb hom sib txawv: Hauv hom "Manual", ntawm "Timer" lossis nyob ntawm qhov kev vam khom ntawm "Kev tshwm sim". Siv cov qauv ua ke rau "Start" thiab "Stop". Thov nyeem tshooj “Operating CMOS-MEA-Control” kom paub meej cov ntaub ntawv.

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CMOS-MEA-CONTROL SOFTWARE
Spike Server
Tus "Spike Server" tso cai rau cov kwj pom cov kab mob thiab cov xwm txheej rau lwm cov ntawv thov ntawm tib lub khoos phis tawj lossis rau lwm lub khoos phis tawj hauv lub network. Tus neeg siv khoom tau txais cov ntaub ntawv thiab tuaj yeem ua raws li qhov xav tau ntawm cov neeg siv khoom. Peb muab qhov chaws rau cov neeg siv khoom zoo li no hauv C#, Matlab thiab Python. Thauj khoom

Saib xyuas lub xeev tam sim no ntawm lub peev xwm ntawm cov ntaub ntawv tau txais lub computer ntawm "Load" qhov rai. Saib CPU load hauv "CPU Usage", thiab tseem ceeb tshaj plaws muaj "Disk Space" ntawm tus tsav siv rau kev kaw.
Nco ntsoov tias CMOS-MEA5000-System generates mus txog 220 MB ib ob thaum kaw nrog ntau tshaj sample nqi!
Cov "Cov Ntaub Ntawv Qhov Chaw" thiab "Cov Ntaub Ntawv", "Cov Cuab Yeej Ua Haujlwm", "Spike Tool" thiab "Spike Server" qhov rai tso cai rau pom cov khoom ntawm cov software uas tsim cov nqi ntawm cov khoom thauj tam sim no. Txhua lub cuab yeej muaj nws tus kheej kab ntawm cov ntaub ntawv pob los ua cov txheej txheem. Yog hais tias lub load ntawm lub processor yog cov cuab yeej siab yuav tsis tuaj yeem ua nws cov ntaub ntawv thiab cov ntaub ntawv kab ntawm cov cuab yeej loj hlob. Lub xeev ntawm cov kab no yog qhia rau txhua lub cuab yeej sib cais.
Sensor Tam sim no

Saib "Sensor Tam Sim No" ntawm CMOS nti. Lub sensor tam sim no yog ntsuas raws li qhov sib npaug ntawm tag nrho cov tam sim no tshaj tag nrho 4225 sensors. Tag nrho cov tam sim no yuav tsum tsis txhob loj tshaj li 300 mA, txwv tsis pub lub nti yuav puas.

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CMOS-MEA-CONTROL SOFTWARE
Log

"Log" file lub qhov rais qhia cov kev sim tsis tu ncua hauv online. Hauv "Log" file tag nrho cov software ua haujlwm thaum lub sijhawm sim

muaj ntaub ntawv. Lub "Log" tuaj yeem khaws cia thiab xa tawm ua "*.xml" file los ntawm nias lub disc

icon.

Cov ntaub ntawv tso saib
Kev ua si
Vim muaj ntau cov channels, nws tsis tuaj yeem qhia cov ntaub ntawv nyoos ntawm 4225 channels ib txhij. Yog li ntawd, kev ua ub no yog pom nyob rau hauv lub sij hawm bins nyob rau hauv ib tug cuav xim code. Qhov no tso cai rau tus neeg siv txheeb xyuas thaj chaw nquag thiab tsom mus rau lawv hauv thaj chaw nyiam. Tso cov ntaub ntawv "Kev Ua Haujlwm" hauv peb hom: "Max Amplitude", "Mean AmpLitude" thiab "Spike Count".

Saib qhov ua tiav CMOS-MEA electrode array hauv "Kev Ua Haujlwm" qhov rai. Cov phiaj xwm "Kev Ua Haujlwm" qhia tag nrho 65 x 65 sensors ntawm CMOS nti ua ib pixel txhua. Xaiv ntau "Cov cheeb tsam ntawm kev txaus siab ROIs" raws li xav tau los tso saib thaj chaw zoo siab tshaj plaws ntawm CMOS array nkaus xwb. Nyob rau hauv cov screenshots hauv qab no cheeb tsam ntawm kev txaus siab yog tso tawm kom pom. Offset Correction Siv lub checkbox "Offset Correction" kom muab tag nrho cov qib electrode mus rau xoom.
Tsim cov cheeb tsam ntawm kev txaus siab ROIs
Thov cia tus nas laug khawm nias kom kos ib daim duab plaub hauv "Kev Ua Haujlwm" qhov rai los tsim ib cheeb tsam txaus siab. Ib kab dashed hauv xiav qhia txog ciam teb ntawm ROI. Cov xim ntawm cov duab plaub tig mus rau xim dub, thiab tus naj npawb ntawm ROI tshwm nyob rau sab xis sab xis. Los yog tsim ROI los ntawm txhaj rau ib qho ntawm cov haujlwm maxima. Hloov kho thaj tsam ntawm kev txaus siab yog nyem rau ntawm ciam teb kom txog rau thaum ob lub xub tshwm sim txav ciam teb. Tshem tawm ROI los ntawm txhaj rau ROI dua. Cov cheeb tsam ntawm kev txaus siab raug tso tawm nyob rau hauv cov nplooj ntawv sib cais. Txhawm rau hloov ntawm ib qho ROI mus rau lwm qhov, thov siv cov tab hauv qab "Single View"ntawm lub qhov rais "ROI". Tseem ceeb: Nws tsis raug tso cai los sib tshooj cheeb tsam ntawm kev txaus siab! Lub sensor channels suav nrog hauv cheeb tsam txaus siab tau pom tam sim ntawd hauv "ROI" cov ntaub ntawv qhov rai ntawm ib sab.

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CMOS-MEA-CONTROL SOFTWARE

Lub toolbar ntawm "Kev Ua Haujlwm" muab faib ua ob ntu: Ib feem saum toj thiab ib feem hauv qab "Kev Ua Haujlwm" zaub. Upper Toolbar ntawm "Kev Ua Haujlwm" Qhov rai

Nyem rau "Parameter Selection Icon" icon los txiav txim seb hom kev ua haujlwm ntawm lub sensor yuav tsum raug tso tawm: Qhov siab tshaj plaws "MaxAmplitude" los yog "MeanAmplitude los yog "SpikeCount". Nyob ntawm seb koj xaiv, lub toolbar ntawm ib sab hloov.
Cov kev xaiv xaiv yuav raug pom nyob rau hauv lub qhov rais kev ua ub no raws li tsis tseeb xim zajlus nyob rau hauv lub sij hawm bins. Txhais cov xim ntawm daim ntawv qhia hauv "Sensor Array View Settings" dialog.

“Reset Offset”

Siv lub checkbox "Offset Correction" kom muab tag nrho cov qib electrode mus rau xoom, yog tias tsim nyog. Nias lub pob kom xoom cov qib electrode tom qab drifting dua.

Tseem ceeb: Qhov "Kev Kho Kho Kho Kho" yuav tsis hloov cov ntaub ntawv nyoos, uas tau sau tseg! Qhov no tsuas yog cuam tshuam cov ntaub ntawv tso tawm!

Rau qhov "SpikeCount" parameter, spikes raug kuaj ib tus zuj zus rau txhua tus channel los ntawm qhov pib hla. Qhov chaw pib yog xam raws li tus qauv sib txawv ntawm lub suab nrov rau cov channel, raws li tus neeg siv tau hais tseg. Qhov xwm txheej tuaj yeem hloov pauv nrog lub npov nce-down ntawm ib sab ntawm qhov kev xaiv parameter icon. Kev suav ntawm tus qauv sib txawv tuaj yeem hloov kho txhua lub sijhawm nrog lub cim "Hloov kho Std.

Dev. Ntsuas".

Qhib lub "Activity Tool Settings" dialog los ntawm txhaj lub icon . Cov txheej txheem yooj yim, xws li hloov tshiab ntawm lub sijhawm bins thiab tshawb pom lub sijhawm tuag tuaj yeem hloov pauv hauv "Kev Ua Haujlwm Cov Cuab Yeej" dialog.

Qhov "Hloov tshiab" parameter txhais qhov loj ntawm lub sijhawm bins thiab tus nqi refresh ntawm cov zaub. "Tshaj" thiab "Kev Tshawb Nrhiav Tuag Lub Sijhawm" yog qhov tseem ceeb yog tias cov suav suav tau tshwm sim. Spike suav yuav raug sau rau lub sijhawm xaiv, cov xwm txheej hla qhov kev tshawb pom qhov pib nyob rau hauv qhov xaiv "Nrhiav Tuag Sijhawm" tom qab qhov xwm txheej dhau los yuav raug tsis quav ntsej. Qhov no txhais tau hais tias thaum saib cov suav suav, cov zaub yuav pib qhia cov ntaub ntawv nrog ob peb vib nas this ncua tom qab pib cov ntaub ntawv tau, nyob ntawm qhov "Tswj" qhov chaw.

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CMOS-MEA-CONTROL SOFTWARE

Hauv qab toolbar ntawm "Kev Ua Haujlwm" Qhov rai

Yog hais tias tus nas pointer nyob rau hauv daim ntawv qhia, lub coordinates ntawm cheeb tsam tus nas taw tes ntawm yog qhia nyob rau sab laug ntawm toolbar hauv qab lub qhov rais zaj duab xis.

Txhais cov xim ntawm "Daim Ntawv Qhia Kev Ua Haujlwm" los ntawm nias lub cim thib ob

hauv toolbar hauv qab no. "Sensor Array View Kev teeb tsa”

dialog tshwm. Qhib lub checkbox "Invert" los hloov cov xim ntawm daim ntawv qhia kev ua si.

Los ntawm lub neej ntawd, ib cheeb tsam ntawm kev txaus siab (ROI) yog txhais ntawm "Kev Ua Haujlwm" zaub. Cov ntaub ntawv nyoos ntawm tag nrho cov sensors hauv ROI tau nthuav tawm kom meej. Qhov loj thiab txoj hauj lwm ntawm ROI tuaj yeem hloov tau los ntawm kev rub nrog nas. Nws muaj peev xwm txhais tau ntau tshaj ib cheeb tsam ntawm kev txaus siab. Nyem cov nyees khawm ntxiv lossis tshem tawm ROI ntxiv. Thov kos ib daim duab plaub nrog tus nas hla ntawm qhov sib txawv ntawm cov sensor array los txhais thaj tsam ntawm ROI. Yog tias muaj ntau tshaj li ib cheeb tsam ntawm kev txaus siab, lawv tau tso tawm ntawm nws tus kheej hauv cov nplooj ntawv tabbed. Thov nyeem ntxiv tshooj “Tsim thaj tsam ntawm kev txaus siab ROIs” saum toj no.
Optionally nws muaj peev xwm los txhais thaj tsam ntawm ROI nrog "Teeb ROI Cursor" dialog. Txhais qhov "Position", "Size", thiab "xim" ntawm tus cursor ROI. Qhia lossis zais cov plaub hau hla ntawm "Show Cross Hairs" kos lub thawv hauv qab no thiab "Enable" tus cursor ntawm lub npov saum toj no.

Txhais tus nqi ntawm daim ntawv qhia xim hauv microvolt nrog lub npov nce-down "Range" ze rau qhov siab tshaj plaws amplitude lossis spike zaus ntawm qhov xav tau cov cim.

. Tus nqi siab tshaj plaws yuav tsum yog

Cov xim qhia txog cov kev xaiv uas tau xaiv hauv "Kev Ua Haujlwm" zaub. Raws li pom hauv daim duab hauv qab no, kev ua ub no yog pom raws li cov xim xim.

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CMOS-MEA-CONTROL SOFTWARE
Sensor Array Tool

Lub "Sensor Array Tool" koom ua ke peb lub luag haujlwm uas cuam tshuam nrog kev tswj hwm ntawm CMOS-MEA nti.
Ua ntej ntawm tag nrho cov, koj tuaj yeem manually recalibrate koj sensors, yog tias tsim nyog. Qhov thib ob, cov cheeb tsam ntawm cov sensors tuaj yeem xaiv manually lossis tau txais kev tsom mus rau cov cheeb tsam txaus nyiam. Thib peb, thaum tsim nyog, qhov chaw khiav hauj lwm ntawm cov sensors tuaj yeem rov qab los ntawm kev teeb tsa lub rooj vag vol.tage ib zaug lossis hauv hom rov ua dua tshiab. Thaum kawg ntawm lub kaw lus calibration khiav cov kev hloov pauv ntawm tag nrho cov sensors uas tau tso tawm kom pom qhov zoo ntawm lub sensor nti. Thov xaiv ib qho ntawm peb hom los ntawm cov ntawv qhia zaub mov nco: "Calibration", "Sensor Selection" thiab "Sensor Reset".
Calibration

Raws li tau hais los saum toj no cov xwm txheej ntawm cov nti sensor tuaj yeem kwv yees los ntawm kev hloov pauv yam tau txais thaum lub sijhawm ntsuas qhov system. Txhua qhov ntsuas qhov ntsuas ntsuas tus nqi yog sawv cev los ntawm cov xim coded dot. Qhov tseeb qhov tseem ceeb nyob ntawm tus sensor nti. Cov qauv ntawm qhov muaj nuj nqis no yuav tsum yog hom random nrov qauv.

Ib qho qauv hauv cov qauv nrov nrov no tuaj yeem qhia tau tias muaj teeb meem. Rau example, ib cheeb tsam dawb yuav taw tes rau ib pawg ntawm cov sensors tsis raug. Los yog txoj kab ncaj nraim hla tag nrho cov nti qhia txog kev sib cuag tsis zoo rau cov nti sensor.

Thov tso tseg qhov kev sim thiab ntxuav cov pins ntawm lub taub hautage. Dawb pixel taw tes rau ib qho teeb meem ib leeg sensor, qhov twg calibration

tsis tau. Sim rov ua cov txheej txheem los ntawm txhaj rau "Calibrate" icon hais kom ua raws li hauv "CMOS-MEA Diagnose" dialog.

rau calibration ntawm CMOS sensors. Qhov no yog tib yam

Rau calibration ib sine teeb liab nrog 70 Hz thiab 3 mV yog teem tawm tsam ib tug sine teeb liab los ntawm lub stimulator sab hauv.

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CMOS-MEA-CONTROL SOFTWARE
Kev Xaiv Sensor
Qhov "Sensor Selection" module tso cai rau tus neeg siv xaiv thaj chaw ntawm kev txaus siab ntawm tus kheej lossis txiav. Nws yog ib qho tseem ceeb kom txo tau cov ntaub ntawv xa mus rau lub khoos phis tawj thiab hard disk thaum cov haujlwm raug txwv rau ib lossis ntau qhov chaw ntawm lub sensor array. Nqa cov kev faib tawm uas tau txais los ntawm "Kev Ua Haujlwm" lossis "Spike Tool" thiab siv nws los txheeb xyuas thaj tsam ntawm kev txaus siab.

Upper Toolbar ntawm "Sensor Array Tool" Qhov rai
Thov rub koj xaiv cov cheeb tsam uas txaus siab nrog lub "Download tau xaiv Sensors rau cov ntaub ntawv tau txais" khawm rau lub computer. Siv lub pob "Fetch Spike Count from Spike Tool" khawm kom import cov ntaub ntawv los ntawm "Spike Tool" thiab nyem rau ntawm "Fetch Activity from Activity Tool" khawm kom import cov ntaub ntawv los ntawm "Kev Ua Haujlwm" cuab yeej. Txo Toolbar ntawm "Sensor Array Tool" Qhov rai
Xaiv qhov ua tiav array nrog "Xaiv tag nrho Sensor Array" khawm. Siv lub pob "Automatic Sensor Selection" yog tias koj tsis mus xaiv thaj chaw txaus siab ntawm tus kheej. Lub cuab yeej tsis siv neeg xaiv cov chaw nyob ib puag ncig cov kev ua si siab tshaj. Thov txheeb xyuas qhov loj me ntawm thaj tsam ntawm kev txaus siab los ntawm lub npov nco-down, "Size 3 x 3" sensors lossis "Size 5 x 5" sensors. Deselect tag nrho cov cheeb tsam ntawm kev txaus siab nrog lub "Tshem tawm xaiv Sensors" khawm. Tsis tas li ntawd, rau kev xaiv tsis siv neeg ntawm ROIs koj tuaj yeem ntxiv ROIs lossis hloov kho lossis tshem cov sensor arrays manually. Thov nyeem nyob rau hauv tshooj “Cov Ntsiab Lus Tseem Ceeb” ntawm nplooj ntawv 18 kab lus “Tsim thaj tsam ntawm kev txaus siab ROIs”.

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CMOS-MEA-CONTROL SOFTWARE
Pib dua Sensor
Rau cov ntaub ntawv ntev heev los yog rau kev sim tej yam kev mob uas ua rau muaj zog drifts ntawm lub sensors nws yuav tsum tau rov pib dua lub sensors khiav hauj lwm taw tes. Kev rov pib dua ntawm cov sensors tuaj yeem tswj tau manually lossis txiav ntawm cov neeg siv lub sijhawm. Lub sij hawm ntawm qhov pib dua yog tau txais thiab khaws cia raws li cov ntaub ntawv channel thiab kuj yog cov xwm txheej ua ke nrog cov ntaub ntawv sensor. Cov ntaub ntawv no tuaj yeem siv los tshem tawm cov theem pib dua los ntawm kev txheeb xyuas. Txhawm rau rov pib dua cov ntsiab lus ua haujlwm sensors, xaiv "Sensor Reset" module los ntawm "Sensor Array Tool" toolbar. Nias lub pob pib pib dua cov sensors. Lub sijhawm rov pib dua tuaj yeem teeb tsa nrog tus lej tswj. Kos lub "Rov dua" tswj kom rov ua dua lub sensor pib dua nrog lub sijhawm muab. Lub cuab yeej "Sensor Reset" tsa cov teeb liab ntab rov qab mus rau qhov chaw ua haujlwm. Lub sijhawm luv luv no pom hauv cov ntaub ntawv nyoos raws li cov kauj ruam muaj peev xwm. Lub sijhawm caij nyoog ntawm qhov rov pib dua yog sau ua ke nrog cov ntaub ntawv nyoos raws li cov ntaub ntawv channel lossis raws li cov xwm txheej. Example: "Sensor Reset" yam tsis muaj kev tawm tsam

Cov example saum toj no ntawm sab laug qhia tau hais tias lub sensor pib dua nrog lub sijhawm ntawm 1 ms thiab tsis muaj kev tawm tsam. Lub lim ntawm 10 Hz yog siv.

Cov example saum toj no ntawm sab xis qhia tau hais tias lub sensor pib dua nrog lub sijhawm ntawm 1 ms thiab tsis muaj kev tawm tsam yam tsis muaj lim.

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CMOS-MEA-CONTROL SOFTWARE
Example: "Sensor Reset" nrog kev tawm tsam

Cov example saum toj no ntawm sab laug qhia tau hais tias lub sensor pib dua nrog lub sijhawm ntawm 1 ms, nrog 10 Hz lim thiab nrog kev tawm tsam. Cov example saum toj no ntawm sab xis qhia tau hais tias lub sensor pib dua nrog lub sijhawm ntawm 1 ms, tsis muaj lim thiab nrog kev tawm tsam. Cov nyhuv ntawm kev tsuj thiab ntawm 10 Hz high pass lim yog pom tseeb pom nyob rau hauv kev sib piv ntawm plaub screenshots saum toj no. Nyem lub checkbox "Artefact Suppression" txhawm rau lim tawm cov khoom cuav.
Spike Tool

Cov "Spike Tool" yog siv rau kev txiav txim siab online ntawm qhov tsis sib xws kom tso cai rau kev ua haujlwm nyob ntawm kev kaw. Qhov no muaj txiaj ntsig zoo thaum cov theem nquag tau kos rau hauv cov theem tsis ua haujlwm ntev. Siv cov yam ntxwv no tsuas yog lub sijhawm nyob ib puag ncig cov theem nquag tau khaws cia rau hauv disk.
Spike Tool Upper Toolbar

Txhais qhov "Threshold" rau kev tshawb nrhiav qhov dav los ntawm lub npov poob: "Zoo", "tsis zoo" lossis "Tsis Txaus". Xaiv tus qauv sib txawv "StdDev" los ntawm tus lej nce-down box. Siv qhov "Hloov kho StdDev Measure", yog tias tsim nyog.

Siv lub checkbox "Offset Correction" kom muab tag nrho cov qib electrode mus rau xoom, yog tias tsim nyog. Nias lub pob "Reset Offset" cov qib electrode tom qab drifting dua.

rau xoom

Tseem ceeb: Qhov "Kev Kho Kho Kho Kho" yuav tsis hloov cov ntaub ntawv nyoos, uas tau sau tseg! Qhov no tsuas yog cuam tshuam cov ntaub ntawv tso tawm!

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CMOS-MEA-CONTROL SOFTWARE
Example: Cov ntaub ntawv tso saib "Spike Overlay" nrog "Offset Correction" ntawm sab laug thiab tsis muaj "Offset Correction" ntawm sab xis.

Nyem rau "Qhib Chaw Dialog" khawm

qhib qhov "Spike Explorer Settings" dialog.

Spike Detection
Thov txheeb xyuas ntau yam parameter rau qhov kev kuaj pom. Lub suab nrov yog siv los ua lub hauv paus los xam qhov pib ntawm cov spikes uas yuav tsum tau kuaj pom.

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CMOS-MEA-CONTROL SOFTWARE
Noise Measure Section "Noise Measure" parameter: Xaiv seb koj puas xav teem "Standard Deviation" lossis "Median / Absolute Deviation" rau kev ntsuas suab nrov. "Tim Sijhawm": Peb qhov kev txwv muaj los txheeb xyuas lub sijhawm uas lub suab nrov yuav tsum ntsuas rau kev siv nws los ua lub hauv paus rau qhov pib. Qhov ntsuas "Nruam" yog tawm tsam qhov ntsuas lub sijhawm. Siv ib qho "Single Interval" lossis "Repeat Interval". Kev ntsuas tsis tu ncua ntawm lub suab nrov raws li lub hauv paus rau qhov kev sib tw spike yog tsim nyog yog tias cov ntaub ntawv nyoos yog drifting. Ua li no, qhov chaw pib rau cov spikes yuav tau yoog. Txhawm rau rov ua qhov ntsuas suab nrov nyob rau hauv ib ntus kuj tsim nyog rau kev sim tsis tu ncua. Thov txhais cov naj npawb ntawm "Rov ua dua" thiab "Lub Sij Hawm" ntawm lub sijhawm nyob rau hauv milliseconds ntawm lub npov nce-down.
Kev Tshawb Nrhiav Ntu "Kev Tshawb Nrhiav" parameter: Txhais "Thiab Hom" los ntawm lub npov poob, "Zoo", "tsis zoo" lossis "Tsis Muaj". Nws kuj tseem tuaj yeem txhais qhov ntsuas no hauv cov cuab yeej bar ntawm "Spike Tool" zaub. Xaiv "Threshold" los ntawm 0 txog 99 ntawm lub npov nce-down. Txhais lub "Nrhiav Tuag Sijhawm" hauv ms.
Spike Cutout Section "Spike Cutout" parameter: Nyem lub thawv "Extract Waveform" yog tias tsim nyog. Txhais qhov "Waveform Alignment" los ntawm lub npov nco-down, yog tias lub thawv kos tau qhib. Txhais qhov "Pre thiab Post Interval" hauv milliseconds ntawm lub thawv nce-down. Spike Tool qis dua Toolbar
Saib cov kev tswj xyuas ntawm tus kws qhia ntawv hauv cov zaub raws li feem ntau. Xaiv cov sensors tau txais nrog "Tsis Siv Neeg Sensor Xaiv" khawm lossis tshem tawm lub sensor los ntawm nias lub pob "Remove Sensors". Txhais qhov "Minimum Spike" tus nqi thiab "Range" ntawm lub thawv nce-down. Nyem rau "Qhib Sensor Xaiv" khawm kom qhib lub dialog.
Thov txhais qhov "Txoj Kev rho tawm", "Smoothing" thiab "Minimum Spikes".

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CMOS-MEA-CONTROL SOFTWARE
Nyem rau "Qhib Chaw Dialog" khawm qhib lub dialog.
Txhais qhov parameter rau lub sensor array: "Hloov tshiab" ntawm lub array thiab cov tsub zuj zuj "Tau" hauv ms thiab xim ntawm cov zaub hauv "Color Map". Spike Tool Event Qhov rai
Saib tus nqi nce siab nyob rau hauv kev vam khom ntawm lub sijhawm hauv "Spike Tool Event" qhov rais thiab kho qhov chaw pib kom ua rau muaj xwm txheej los ntawm kev txav lub bar liab nrog tus nas. Thaum tus nqi nce siab hla qhov pib ntawm qhov zoo, qhov kev tshwm sim tshwj xeeb "Spike Rate Threshold" raug tsim thiab xa mus. Thaum tus nqi nce siab hla qhov pib ntawm qhov tsis zoo ib qho kev tsis zoo "Spike Rate Threshold" yog tsim thiab xa tawm. Cov xwm txheej tau pom zoo li daim duab peb sab hauv txiv kab ntxwv (kev tshwm sim pib) thiab xiav (tshem qhov tshwm sim) nyob rau hauv qab ntawm cov kab hauv "Spike Tool Event" view. Tsis tas li ntawd, lawv raug xa mus rau "Recorder" rau kev xaiv siv. Tus nqi spike yog xam los ntawm tag nrho cov sensors los yog rau ib tug subset ntawm sensors. Yog tias tsis muaj cov sensors raug xaiv tag nrho cov sensors raug siv. Txhawm rau ua qhov kev ntsuam xyuas tshwj xeeb, tsuas yog xaiv cov sensors uas qhia tus cwj pwm koj nyiam. Txwv tsis pub qhov kev tshuaj ntsuam rau ob peb lub ntsuas ntawm lub cev txawv txav txawv txav koj tuaj yeem txo qhov "suab nrov" los ntawm tag nrho lwm cov sensors nrog kev ua haujlwm tsis sib xws. Thov nyeem tshooj “Kev Sau” hauv “Kev Ua Haujlwm CMOS-MEA-Control Software”.

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CMOS-MEA-CONTROL SOFTWARE
Kev txhawb siab
Thov nyeem tshooj “Kev Txhawb” kom paub meej. Cov hauv taub hautage ntawm CMOS-MEA5000-System integrated stimulator muab qhov muaj peev xwm ntawm stimulating ntawm peb txawv stimulation qauv ntawm cov neeg siv txhais stimulation electrodes. Analog Channels
Yim "Analog Channels" muaj los sau cov cim analog ntxiv. Pib thiab nres cov analog tso tawm cais. Siv lub toolbar los kho qhov zoom zoom thiab lub sijhawm rau cov ntaub ntawv. Thov nyeem ntxiv tshooj “Cov Nta Qhia dav dav”. Digital Port Events

Cov xwm txheej digital tuaj yeem tsim tawm ntawm TTL pulses tuaj rau ntawm "Dig In" ports ntawm lub rooj sib tham. Hauv "Kev tshwm sim" lub thawv, xaiv tus lej tshwm sim. Rau txhua qhov xwm txheej, qhov "TRUE" tuaj yeem txhais tau. Qhov xwm txheej tuaj yeem yog TTL ntawm ib qho ntawm 16 cov khoom siv digital, txawm tias qhov nce lossis poob qis. Nws kuj tuaj yeem ua ke ntawm cov khoom sib txawv, uas tuaj yeem ua ke los ntawm "AND lossis LOS YOG" mob. Hauv example qhia saum toj no, ib qho kev tshwm sim Tsis muaj 4 yuav raug tsim tawm ntawm qhov nce ntawm lub TTL los ntawm me ntsis 2 lossis ntsis 3 ntawm cov channel digital.
Tsuas yog TTL cov teeb liab raug lees txais raws li cov teeb liab tawm tswv yim ntawm cov khoom siv digital. Siv cov ntawv qhia zaub mov nco-down ntawm lub cuab yeej bar hauv qab no los kho lub sijhawm rau cov ntaub ntawv tso tawm. Tshem tag nrho cov xwm txheej nrog lub pob "X". Pib thiab nres cov zaub cais.

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CMOS-MEA-CONTROL SOFTWARE
Paub meej View: Thaj Chaw Txaus Siab

Txhais ib lossis ntau thaj tsam ntawm kev txaus siab hauv "Kev Ua Haujlwm" tso rau ntawm sab laug ntawm cov ntawv qhia zaub mov tseem ceeb. Yog tias muaj ntau tshaj ib qho ROI, thov saib cov nplooj ntawv tab hauv cov ncauj lus kom ntxaws view. Tsis txhob xaiv ntau dhau sensors rau cov ncauj lus kom ntxaws view, vim hais tias tso saib ib leeg sensors xav tau ntau lub computer kev ua tau zoo.

Nyob rau sab xis ntawm lub qhov rais loj, koj muaj cov ncauj lus kom ntxaws txog cov electrodes ntawm cov cheeb tsam uas tau xaiv tam sim no txaus siab. Siv lub toolbar los kho qhov zoom zoom thiab lub sijhawm rau cov ntaub ntawv tso tawm. Ib leeg Sensor View

Xaiv ib qho ntawm cov electrodes ntawm ROI kom pom ze dua.
Pib thiab nres qhov "Siv Sensor View” cais kom txuag tau lub computer kev ua tau zoo los yog kom pom ze dua. Siv lub toolbar los kho qhov zoom zoom thiab lub sijhawm rau cov ntaub ntawv. Thov nyeem ntxiv tshooj “Cov Nta Qhia dav dav”.

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CMOS-MEA-CONTROL SOFTWARE

Ua haujlwm CMOS-MEA-CONTROL SOFTWARE Teem Ntaus Ntaus

Yog tias lub cuab yeej tsis raug lees paub tom qab pib CMOS-MEA-Control Software, thov nyem rau ntawm "Settings" icon

hauv

"Data Source" qhov rai rau "Teeb Ntaus" dialog thiab xaiv cov cuab yeej txuas nrog CMOS. Qhov no tuaj yeem tshwm sim thaum thawj zaug siv ntawm ib qho tshwj xeeb

cov ntaub ntawv tau txais lub computer, lossis tom qab hloov kho dua tshiab.

Xaiv qhov "Sample Rate" los ntawm cov ntawv qhia zaub mov. Lub cheeb tsam ntawm kev txaus siab yog txwv thaum kaw nrog siab sample tus nqi, ntau tshaj 25 kHz. Xaiv ib mus rau yim "Analog Channel" thiab "Digital Channel", yog tias xav tau.
Cov kauj ruam tom ntej yog los txhawb lub zog CMOS-MEA nti.

Thov nyem rau ntawm CMOS-MEA icon nyob rau hauv "Cov ntaub ntawv Source" kom lub zog lub nti. Hom nti, qhov kev ncua deb ntawm qhov chaw mus rau qhov chaw ntawm lub kaw lus electrodes thiab tus lej ID raug txheeb xyuas, rau example “nMOS16”. "n" qhia hom nti, "n" txhais tau tias tsis zoo, "p" yog hom doped chip zoo.

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CMOS-MEA-CONTROL SOFTWARE
Calibration ntawm CMOS-MEA5000-System Muab CMOS-MEA nti rau hauv taub hautage thiab npog nws nrog lub chamber tsaus nti kom tshem tawm qhov cuam tshuam ntawm lub teeb. Txhua zaus CMOS-MEA nti muab tso rau hauv amplifier thiab nti yog powered, nws yuav tsum tau calibrated. Feem ntau, kev sib xyaw ua ke tsis siv neeg calibration raws tu qauv yog txaus. Muaj tsawg zaus, qhov tsis siv neeg calibration ua tsis tiav. Tom qab ntawd nws kuj tseem tuaj yeem ntsuas lub CMOS-MEA nti manually. Ob qho kev xaiv tau piav qhia ntau ntxiv hauv qab no.
Tsis siv neeg Calibration CMOS-MEA chips yog cov khoom siv nquag thiab yuav tsum tau hloov thiab tawm kom raug, txwv tsis pub lawv yuav raug kev puas tsuaj. Thov nyem lub MEA array icon nyob rau hauv "Cov ntaub ntawv Source" qhov rai kom lub zog lub nti nce los yog nqis. CMOS-MEA chips yuav tsum tau ntsuas ua ntej txhua qhov kev siv. Lub calibration khiav cia li. Feem ntau, nws yuav siv li ob lossis peb feeb.

Tom qab lub nti yog powered lub automatic system calibration dialog yog qhia. Pib lub kaw lus tsis siv neeg calibration lossis kaw lub dialog kom calibrate lub kaw lus manually.

Cov kauj ruam tseem ceeb hauv qab no rau calibration yog ua tiav. Thov saib cov txheej txheem teev tseg file saib xyuas cov txheej txheem.
Qhov system calibration muaj plaub kauj ruam sib law liag:
1. Teeb sensor ua haujlwm taw tes. 2. Tos kom txog thaum lub teeb liab nres. 3. Kho ADC offset. 4. Calibrate sensors.
Pib thiab nres qhov calibration ntawm "Automatic System Calibration" dialog nrog rau cov nyees khawm. Nias lub xub ob lub cim khawm kom hla ib qho ntawm cov kauj ruam calibration. Nyob rau hauv daim teb hauv qab no tag nrho cov kauj ruam calibration yuav raug teev tseg.
Calibration Kauj ruam los ntawm kauj ruam
1. Optimizing lub sensor ua haujlwm taw tes

Qhov "Operating Point" yog txawv nyob ntawm seb hom CMOS-MEA nti. Nws yuav raug teem cia li.

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CMOS-MEA-CONTROL SOFTWARE
2. Kev tswj ntawm lub Sensor Drift
Tom qab CMOS-MEA nti tau siv zog thiab qhov taw tes ua haujlwm tau teeb tsa, cov teeb liab sensor yuav ntws mus ib ntus. Thaum lub sij hawm "Sensor Drift" kauj ruam, cov teeb liab ntawm tag nrho cov sensors yog ntsuas, thiab cov nqes hav raug xam. Thaum txoj kab nqes nruab nrab ntawm tag nrho cov sensors mus rau hauv qab ntawm qhov chaw pib ua ntej, cov teeb liab pom tau tias ruaj khov, thiab cov kauj ruam tom ntej tuaj yeem pib. Cov teeb liab sensor yuav raug saib xyuas kom txog thaum lawv ruaj khov. Yog hais tias lub drifting ntawm tag nrho cov sensors tsawg, cov kauj ruam tom ntej calibration tuaj yeem tshwm sim. Lub sij hawm ntawm lub sensor stabilization tuaj yeem sib txawv ntawm CMOS-MEA chips thiab nyob ntawm seb lub nti. Feem ntau, cov kauj ruam no yuav tsum tsis txhob siv sijhawm ntev tshaj li ob peb feeb.
3. Kho ADC Offset Nyob rau hauv cov kauj ruam nram qab no calibration ADC (Analog to Digital Converter) offset tus nqi yog kho kom pom qhov pom offset rau tag nrho los yog feem ntau sensor signals. Cov teeb liab yuav tsum nyob ze rau xoom. Yog hais tias qhov nruab nrab ntawm tag nrho cov sensors poob qis dua qhov pib ua ntej, "Adjust ADC Offset" tiav.

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CMOS-MEA-CONTROL SOFTWARE
4. Calibration ntawm Sensors
Nyob rau hauv cov kauj ruam kawg ntawm lub sensor calibration, ib tug siv voltage teeb liab (sine yoj) yog siv rau da dej los ntawm kev siv electrode. Yog tias qhov teeb meem no tsis pom los ntawm cov sensors, thiab yog li tsis pom hauv ib qho channel view, qhov no txhais tau hais tias cov electrodes puas lawm los yog da dej siv electrode puas lawm. Los ntawm txhais tes Calibration Nyem qhov kev xaiv "Setup" nyob rau hauv lub ntsiab ntawv qhia zaub mov qhib lub "Setup CMOS System" dialog. Lub "Setup CMOS System" dialog tso cai rau tswj txhua kauj ruam ntawm lub sensor calibration raws li tau piav saum toj no manually.

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CMOS-MEA-CONTROL SOFTWARE
Lub taub hau
Pib cov ntaubntawv povthawj siv ntawm "Start" khawm. Zaub ntawm hom CMOS-MEA nti. High Pass Filter Siv lub siab pass lim thaum kaw. Tseem ceeb: Rau kev kuaj mob, thov deactivate lub siab pass lim kom pom qhov drift thiab offset! Chip Power Qhov rai
Tom qab lub zog ntawm CMOS-MEA nti, koj yuav pom cov ntaub ntawv hais txog voltage thov rau lub nti, tso tawm hauv lub thawv nce-down. Tus neeg siv tsis raug tso cai los hloov cov voltage qib. Teem Sensor Operating Point Qhov rai

Thov teem lub sensor khiav hauj lwm taw tes rau hauv mV. Tus qauv qhov tseem ceeb rau "Source-Drain" thiab "Source-Gate" rau cov pa chips yog -600 thiab -650 mV. Rau "Low Noise" CMOS-MEA chips tus qauv tus nqi yog: "Source-Drain" -800mV, "Source-Gate" -950mV. Nyem qhov "Teeb" khawm kom rub tawm cov chaw rau lub cuab yeej. Teem AD Converter Qhov rai
Kho lub voltage rau lub ,, Tawm tswv yim Offset "hauv mV manually nrog tus lej nce hauv lub thawv. Nyem qhov "Kho Offset" khawm kom rub tawm qhov chaw rau lub cuab yeej. Hloov tshiab
Nias lub pob "Hloov tshiab" hloov kho lub dialog. Kaw lub dialog nrog "Kaw".

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CMOS-MEA-CONTROL SOFTWARE
Qhov offset rau txhua tus sensor yuav tsum kwv yees li xoom. Lub voltage xav tau rau qhov ADC offset no tso tawm rau hauv mV thiab tuaj yeem hloov kho nrog "Input Offset" lub thawv nce-down.

Koj tuaj yeem soj ntsuam cov txheej txheem hauv ROI zaub thiab hauv ib leeg view. Minimize Floating Artefact Qhov rai

Definition voltage hauv "Source-Bulk" hauv mV ntawm lub thawv nce-down. Download tau cov kev teeb tsa rau lub amplifier nrog "Set". Txhawm rau sau cov ntaub ntawv, koj xav tau qhov chaw "Gate yog ntab". "Floating" txhais tau hais tias lub sensor ntsuas lub da dej nkaus xwb. "Gate to VOP" txhais tau hais tias siv lub voltage mus rau lub rooj vag los txhais cov ntsiab lus ua haujlwm. "Gate to VOP" txhais tau hais tias ib qho voltage yog siv rau lub rooj vag los txhais cov ntsiab lus ua haujlwm. Da Dej Stimulation Qhov rai
Kev ywj pheej los ntawm stimulation ntawm electrodes nws yog ua tau los txhawb lub da dej nws tus kheej. Siv cov yam ntxwv no los txhawb cov ntaub so ntswg lossis kab lis kev cai ntawm CMOS-MEA nti. Txhais lub sine yoj mem tes los ntawm cov thawv "PP Amplitude" hauv mV thiab "Frequency" hauv Hz. Nyem rau ntawm lub pob "Txhim kho" kom pib thiab nres lub da dej stimulation thiab saib cov nyhuv hauv ib leeg view, rau example. Yog hais tias lub sine yoj tsis pom nyob rau hauv ib tug channel view, qhov no txhais tau hais tias tsis muaj cov cim qhia los ntawm CMOS-MEA sensors. Qhov no qhia tau hais tias cov sensors puas lawm los yog da dej siv electrode puas lawm.

Sensor Calibration Qhov rai

Cov input voltage ntawm txhua tus transistor sib xws rau cov sensors txawv. Qhov "Sensor Calibration" muaj nuj nqi teev cov tswv yim voltages mus rau qib sib xws. Lub stimulation ntawm da dej yog thawj kauj ruam, ces cov lus teb teeb liab yog ntsuas thiab ntsuas.

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CMOS-MEA-CONTROL SOFTWARE
Cov ntaubntawv povthawj siv CMOS nti suav nrog 4225 cov ntaubntawv povthawj siv electrodes thiab 1024 qhov chaw stimulation. Lub 16 m interelectrode nrug nti muab qhov kev daws teeb meem siab tshaj plaws. Nrog rau cov electrodes siab, koj tuaj yeem sau los ntawm qhov chaw loj (1 mm² @ 16 m nrug, 4 mm² @ 32 m nrug). Yog li ntawd, koj tuaj yeem pom cov cim qhia los ntawm txhua lub xov tooj ntawm tes thiab txawm tias lub teeb liab nthuav tawm raws li axon, thaum tseem tau txais kev tshaj tawm.view ntawm koj tiav sample. Cov ntaub ntawv yog sampcoj ntawm 25 kHz ib channel. Ua ke nrog kev hloov pauv A / D ntawm 14 ntsis, lub kaw lus ua kom cov ntaub ntawv raug thiab meej. Yog li, CMOS-MEA-Control ua rau cov ntaub ntawv loj heev hauv ntu luv luv. Multi Channel Systems MCS GmbH pom zoo kom muaj lub khoos phis tawj tshiab uas muaj peev xwm nco tau siab. Txawm li cas los xij, nws yog qhov tseem ceeb los txwv lub sijhawm kaw kom huv si, txwv tsis pub qhov chaw disk ntawm cov ntaub ntawv tau txais lub computer yuav dhau sai sai. CMOS-MEA-Control muaj ntau txoj hauv kev los txwv cov ntaub ntawv ntim thiab teeb tsa lub sijhawm kaw raws nraim rau lub sijhawm txaus siab.
1. Thov xaiv cov ntaub ntawv ntws los sau. 2. Txhais cov kev xaiv yuav pib li cas thiab tso tseg cov kev kaw tseg. 3. Kev xaiv: Phau ntawv qhia, timer, txheej xwm (stimulator: on/off/marker, digital event tool). 4. Examples
Thov xaiv cov ntaub ntawv ntws uas koj yuav sau.

Lub dialog "Teeb Ntaus" tshwm tom qab nias lub pob "Settings".

nyob rau hauv lub qhov rais "Data Source". Xaiv qhov "Device" thiab

lub “Sample Rate" los ntawm cov ntawv qhia zaub mov. Xaiv cov "Analog Channels" thiab "Digital Channels" muab los ntawm kho vajtse.

Txawm hais tias koj sau los ntawm cov channel no lossis tsis tau txhais hauv "Recorder" qhov rai ntawm lub thawv kos. Thov saib hauv qab no.

Pib thiab nres lub kaw lus ywj pheej los ntawm kev pib thiab nres lub kaw lus. Thov siv cov ntawv qhia zaub mov nco-down xaiv hom rau pib lub recorder ntawm ib sab tes thiab tso tseg cov ntaubntawv povthawj siv rau lwm tus tes. Kev sib xyaw ua ke ntawm cov qauv sib txawv muaj ntau txoj hauv kev los sau cov ntaub ntawv tsom mus rau lub sijhawm tseem ceeb.

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CMOS-MEA-CONTROL SOFTWARE

Pib thiab nres qhov kaw

"Manual".

Thov nyem qhov "On" / "Off" khawm

nyob rau sab saum toj ntawm lub "Recorder" dialog manually.

Pib thiab Nres Cov Ntaub Ntawv ntawm

"Timer".

Xaiv lub sij hawm "Start" thiab lub sij hawm teev nyob rau hauv vib nas this los ntawm lub up-down box. Qhib lub checkbox "Repeat" yog tias tsim nyog. Pib lub recorder los ntawm nias lub pob "On". Tam sim no lub recorder yog "Armed". Hauv hom no, lub kaw lus tau muab pov tseg thiab tos kom pib ntawm timer lossis kev tshwm sim. Tib qhov xwm txheej "Armed" recorder yuav tshwm sim yog tias tawg ntawm cov txheej txheem kaw tseg.

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CMOS-MEA-CONTROL SOFTWARE

Pib thiab Nres Cov Ntaub Ntawv ntawm

“Event”.

Siv ib qho "Event" raws li qhov pib thiab nres ua rau cov postulates los tsim qhov kev tshwm sim ua ntej. Ib qho "Kev tshwm sim" tej zaum yuav yog ib qho kev tshwm sim digital TTL los ntawm sab nraud lossis sab hauv TTL lub cim teeb liab uas muaj cov qauv kev txhawb nqa.

Yog tias qhov synchronization ntawm CMOS-MEA-System nrog rau lwm cov khoom siv los ntawm sab nraud yog qhov tsim nyog, thov siv qhov "Digital In" ports rau kev tshwm sim nrog TTL pulse. Tsim ib qho kev tshwm sim digital hauv "Digital Port Events" dialog.

Hauv no example qhov ua "Event" tshwm, yog me ntsis 2 lossis ntsis 3 hloov ntawm qis (0) mus rau siab (1).
Thov nyeem tshooj “Kev txhawb siab” kom paub meej txog kev tsim cov qauv kev txhawb nqa. Qhov kev pab cuam TTL cim teeb liab hauv "Set Marker Signal" dialog. Cov cim cim cim yuav raug kaw thiab khaws cia hauv cov ntaub ntawv file rau kev sib piv meej ntawm cov ntaub ntawv thiab cov cim.

Tus qauv stimulation zoo li no, rau example. Cov cim cim cim yog cov kab kab txiv kab ntxwv, pom nyob rau hauv qis qis ntawm cov qauv kev txhawb nqa tiav. Thov txhais lub “Marker Port” rau xa TTL teeb liab.

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CMOS-MEA-CONTROL SOFTWARE

Spike Rate triggered Recording Lub functionality ntawm "Spike Tool" thiab "Spike Recorder" tso cai rau ib qho kev ua si nyob rau hauv cov ntaubntawv povthawj siv. Qhov no yog qhov muaj txiaj ntsig zoo thaum cov theem nquag tau kos rau hauv cov theem tsis ua haujlwm ntev, piv txwv liample rau kev tshawb fawb txog qaug dab peg. Siv cov yam ntxwv no tsuas yog lub sijhawm nyob ib puag ncig cov theem nquag tau khaws cia rau hauv disk.
Lub "Recorder" cuam tshuam nrog cov xwm txheej tsim thiab xa tawm los ntawm "Spike Tool". Cov xwm txheej tau tshwm sim ua daim duab peb sab me me ntawm lub hauv paus ntawm lub sijhawm axis hauv "Spike Tool Event" view. Tus nqi spike yog xam los ntawm tag nrho cov sensors los yog rau ib tug subset ntawm sensors. Yog tias tsis muaj cov sensors raug xaiv tag nrho cov sensors raug siv.

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CMOS-MEA-CONTROL SOFTWARE
Txhawm rau ua qhov kev soj ntsuam tshwj xeeb tsuas yog xaiv cov sensors uas qhia tus cwj pwm koj nyiam.
Kev txwv qhov kev soj ntsuam rau ob peb lub ntsuas ntawm qhov txawv ntawm lub cev sib txawv ntawm qhov sib txawv koj tuaj yeem txo cov suab nrov los ntawm tag nrho lwm cov sensors nrog kev ua haujlwm tsis sib xws. Qhov chaw pib (kab liab) tuaj yeem txav los ntawm nas. Thaum tus nqi nce siab hla qhov pib hauv qhov kev taw qhia zoo (tau loj dua) qhov tshwj xeeb "Spike Rate Threshold (pos)" kev tshwm sim raug tsim thiab xa tawm. Thaum tus nqi nce siab hla qhov chaw pib hauv qhov tsis zoo (tus nqi me me) qhov tshwj xeeb "Spike Rate Threshold (neg)" kev tshwm sim raug tsim thiab xa tawm. Example: Yuav Ua Li Cas Spike Rate Triggered Recording Txhawm rau pib cov ntaub ntawv los ntawm qhov kev ua haujlwm siab dav ntsuas ntawm CMOS nti tsuas yog xaiv "Start by Event" raws li pib hom thiab xaiv "Spike Rate Threshold (pos)" kev xaiv hauv qhov cuam tshuam. lub thawv. Txhawm rau teeb tsa tus nqi "Offset" hauv milliseconds uas ntxiv rau lub sijhawm stamp ntawm qhov kev tshwm sim pib xav tau qhov yuav tsum tau muaj ntawm qhov tsis, uas khaws tag nrho cov ntaub ntawv rau ib lub sijhawm. Cov txiaj ntsig tsis zoo txhais tau hais tias kev kaw cia pib ua ntej lub sijhawm tshwm sim stamp (uas txhais tau hais tias yav dhau los), cov txiaj ntsig zoo pib sau tom qab lub sijhawm tshwm sim stamp. Rau tus nqi ntawm xoom kaw pib raws nraim ntawm lub sijhawm tshwm sim stamp. Txhawm rau tso tseg ib qho kev kaw cia los ntawm qhov kev ua haujlwm hnyav ntsuas ntawm CMOS nti xaiv qhov "Spike Rate Threshold (neg)" qhov xwm txheej raws li qhov xwm txheej nres. Yog tias qhov kev kaw cia tau pib thiab tsis tu ncua, qhov xwm txheej tshwm sim ntxiv yuav raug tsis quav ntsej txog thaum cov kaw lus kaw. Yog li, qhov sib tshooj ntawm qhov sib tshooj ua rau cov kaw lus tsis tuaj yeem ua tau. Lwm qhov kev sib xyaw ua ke muaj txiaj ntsig yuav yog qhov pib los ntawm "Spike Rate Threshold" thiab nres los ntawm "Timer" lossis pib los ntawm "Stimulus Marker" thiab nres nrog "Spike Rate Threshold".

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CMOS-MEA-CONTROL SOFTWARE
Kev Ceeb Toom: Tsuas yog qhov zoo voltages yuav tsum tau siv rau CMOS arrays. Negative voltages yuav puas lub chip. Hloov voltages yuav tsim ib tug tam sim no. Raws li daim ntawv mem tes yog qhov zoo voltaguaamp. Ceeb toom: Tsis txhob siv voltages siab dua 3.4 V, los yog koj yuav ua puas lub CMOS array. Muaj zog voltages yuav ua rau kev tawg ntawm txheej cais. Qhov no yog optically pom ntawm qhov chaw ntawm CMOS nti. Thov saib cov txheej txheem:
Qhov tseem ceeb: qhov kev daws teeb meem ntawm stimulator yog 10 µs nyob rau hauv lub sij hawm axis thiab 105 µV nyob rau hauv voltage. Lub tshuab hluav taws xob tsis muaj peev xwm tso tawm cov pulses luv dua 10 µs lossis qis dua 105 µV, yog li cov pulses no hla! Ib qho tseem ceeb: Txog cuaj qhov stimulation pads (3 × 3) yam tsawg kawg yog xav tau rau kev txhawb nqa zoo. Tus nqi empirical no siv tau rau retina, tsis muaj ntaub ntawv tseem muaj rau cov kab lis kev cai ntawm tes. Nyob rau hauv sib piv rau MEAs li niaj zaus, cov neeg nyob sib ze cov ntaubntawv povthawj siv feem ntau yuav tsis saturate thaum lub sij hawm stimulation, yog li cov ntaubntawv povthawj siv nyob ze ze rau qhov chaw stimulation yog ua tau.
Lub qhov rais Stimulation

Tool Bar

Cov cuab yeej bar nyob rau hauv lub qhov rais "Stimulation" yog muab faib ua peb seem: ib tug qhia txoj hauj lwm, tswj rau ib tug stimulus qauv, thiab kev tswj uas cuam tshuam rau tag nrho peb cov qauv stimulus. Kev ua haujlwm ntawm txhua lub cim tau piav qhia los ntawm cov cuab yeej taw qhia, thaum koj txav tus nas hla lub cim.
Thawj ntu: Qhov taw qhia txoj hauj lwm yuav pab tau los ntawm CMOS-MEA electrodes. Nws qhia qhov tseeb ntawm tus nas.
Tus naj npawb ntawm CMOS-MEA electrodes nyob rau hauv 65 x 65 daim phiaj ua raws li tus txheej txheem tus lej lej rau square grids: Thawj tus lej yog tus lej kab thiab tus lej thib ob yog kab zauv. Rau example, electrode 23 yog nyob rau hauv kab thib ob thiab kab thib peb.

Ntu thib ob: Nyem ib qho ntawm peb cov xim coded "Xaiv qhov xav tau" icons

xaiv tus qauv stimulus ntsig txog

rau kev ua haujlwm ntxiv. Koj tuaj yeem txhais qhov chaw ntawm qhov kev txhawb nqa uas tau xaiv los ntawm txhaj rau ntawm electrodes hauv lub vaj huam sib luag electrode lossis los ntawm kev kos duab

rectangles tshaj qhov xav tau. Txhua qhov stimulation electrode lees txais ib tus qauv stimulus ib zaug. Nws tsis tuaj yeem cuam tshuam cov qauv,

tab sis kom overwrite lawv nrog kev xaiv tshiab lossis tshem tawm cov chaw.

Nyem rau ntawm "Txhais Kev Txhawb" icon

yuav qhib ib tug tshiab dialog ua rau lub ntsiab txhais ntawm lub stimulus qauv raws li piav nyob rau hauv

cov nplooj ntawv nram qab no. Txhawm rau pib stimulation nrog cov qauv xaiv tam sim no nkaus xwb, nyem qhov "Start" icon nyob rau hauv seem thib ob ntawm lub cuab yeej bar.

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CMOS-MEA-CONTROL SOFTWARE

Txhawm rau tshem tawm qhov kev txhawb nqa tam sim no los ntawm txhua qhov chaw, thov siv "Tshwj xeeb xaiv qhov chaw txhawb nqa" ntawm tus qauv xaiv yuav raug tshem tawm.

khawm. Txhua qhov chaw tsim

Txhawm rau rub tawm thiab pib qhov kev txhawb nqa, thov nyem rau ntawm lub pob tom ntej. Txhawm rau tshem tawm txhua qhov chaw txhawb nqa, nyem qhov "Tshem tawm tag nrho

stimulation sites khawm. Download tau, yog tias tsim nyog thiab pib tag nrho cov stimuli ua ke nrog lub "Download thiab pib tag nrho Stimuli" khawm.

Txhawm rau tso tawm qhov "Daim Ntawv Qhia Kev Ua Haujlwm" qhov rai, thov siv lub pob "Display Activity Map".

Tshooj thib peb: Txhawm rau tshem tawm txhua qhov chaw, nyem rau ntawm "Tshem tawm txhua qhov chaw txhawb nqa" icon. Pib tag nrho cov qauv kev txhawb nqa ib txhij ntawm lub "Start" icon hauv ntu no.

Txhawm rau qhib qhov "Txhais Qhov Ntsuas 2" dialog, nyem rau ntawm "Xaiv Kev Txaus Siab 2" icon

. Nyem rau ntawm lub icon "Txhim kho qhov xav tau".

hauv

Lub qhov rais "Stimulation" yuav muaj. Txhua ntawm peb tus qauv stimulus 1 (ntsuab), 2 (xiav) lossis 3 (liab) tuaj yeem txhais tau ntawm nws tus kheej.

Lub dialog rau qhov chaw ntawm cov qauv stimulus yog tsim analog, thov saib, piv txwv liample, lub “Txhais Qhov Kev Ntsuas 2” dialog.

Cov lus "Txhais Ncauj Lus" tau muab faib ua ntu sab sauv thiab sab qis. Sab saum toj suav nrog "Primitives", uas txhais tau tias, cov cim rau cov qauv kev txhawb nqa, xws li kab ncaj nraim, ramp los yog sine waves. Lub cim kawg sawv cev rau ASCII files generated los ntawm ib tug txawv software, rau example MC_Stimulus II, uas tuaj yeem raug xa mus rau hauv CMOS-MEA-Control software. Qhov qis dua ntawm lub dialog qhia tau hais tias

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CMOS-MEA-CONTROL SOFTWARE
"Complete Stimulus Pattern" thiab ib lub cuab yeej bar. Cov kev teeb tsa hauv lub cuab yeej bar cuam tshuam rau qhov ua tiav ntawm cov qauv kev txhawb nqa uas tau tshwm sim tam sim ntawd hauv lub qhov rais rau "Ua tiav Cov Qauv Txheej Txheem". Cov ntawv qhia zaub mov dawb "Tsim Ib Lub Zog" nyob rau hauv nruab nrab ntawm qhov kev sib tham yog tsim los tsim cov qauv kev xav tau los ntawm cov qauv kev txhawb nqa uas tau muab, hu ua "Primitives" lossis rau kev xa tawm ntawm cov qauv tsim tawm sab nraud. Koj tuaj yeem hloov kho txhua tus qauv "Primitive" ntawm tus kheej "Parameter Settings" thiab cov kev hloov pauv yuav tshwm sim tam sim ntawd hauv "Tib Qauv" view thiab tseem nyob hauv view rau "Complete Stimulus Pattern".
Tsim kom muaj tus cwj pwm Stimulus
Nyob rau sab laug ntawm seem sab sauv, koj tuaj yeem xaiv cov qauv kev txhawb nqa uas tau muab. Txav mus rau lub cim tseem ceeb ntawm kev luag thiab poob rau hauv qhov chaw dawb hauv nruab nrab ntawm qhov kev sib tham rau kev tsim tus neeg siv cov qauv stimulation. Ntxiv ntau primitives raws li xav tau. Nws muaj peev xwm hloov qhov sib lawv liag ntawm cov primitives los ntawm luag thiab poob. Txhawm rau rho tawm ib qho ntawm cov qauv, thov tso rau hauv lub thawv. Kev teeb tsa tus cim kos npe

Tsis tas li ntawd, nws muaj peev xwm teeb tsa lub cim cim. Qhib lub "Teeb Marker Signal" dialog los ntawm txhaj rau lub npov "Teeb Marker"

nyob rau hauv lub qhov rais rau parameter nqis. Nyem rau ntawm lub npov "Teeb Cim" thiab nyem "Rov dua" yog tias koj xav rov ua qhov cim cim ntawm txhua lub voj voog ntawm tus qauv stimulation. Kho qhov "Offset" hauv s thiab "Lub Sij Hawm" hauv s ntawm lub cim cim ntawm lub thawv nce-down. Lub teeb liab xim txiv kab ntxwv yuav tshwm nyob rau hauv lub qhov rais rau "Ua tiav Stimulus Pattern".

Modulation ntawm qhov muab Stimulation Patterns

Thaum xub thawj, cov qauv stimulation muab yog qhov qub. Txhawm rau kho qhov tseem ceeb rau koj qhov yuav tsum tau ua, thov nyem rau ntawm lub cim, uas yuav tsum tau ua kom pom hauv xiav xiav. Nyob rau sab sauv ntawm lub dialog qhov teeb tsa tsis rau cov qauv no tshwm nyob rau sab laug thiab ib daim duab ntawm cov duab tiag tiag ntawm tus qauv ntawm sab xis. Tam sim ntawd tom qab hloov tus qauv, qhov kev hloov pauv tau pom nyob rau hauv "Single Pattern" view thiab nyob rau hauv "Complete Stimulus Pattern" view. Txuag tus qauv stimulus raws li lub neej ntawd los ntawm txhaj

lub icon "Save Primitive as Default" icon

ib sab ntawm lub "Marker" kos lub thawv.

Feem ntau ntawm cov kev ntsuas yuav tsum tau kho nrog cov thawv nce-down. Thov nyem rau hauv lub npov nce-down thiab txav lub log ntawm koj tus nas rau kev hloov kho sai hauv cov kauj ruam dav. Siv cov xub los ua kom zoo. Qhov kev hloov pauv tam sim ntawd tshwm sim hauv ib qho qauv thiab hauv qhov ua tiav qhov rais. Overwrite tsab ntawv nyob rau hauv lub up-down box, lub modulation yuav tshwm sim tom qab paub meej tias tus nqi nrog "Enter" los yog tom qab txhaj rau hauv lwm lub thawv. Ua si nrog qhov sib txawv muaj peev xwm hloov kho tso cai rau tsim txhua yam ntawm cov mem tes uas yuav tsim nyog. Tsis tas li ntawd, nws tuaj yeem hloov kho cov duab, thaum rov ua cov pulses hauv kev sib raug zoo rau ib leeg.

Ntau hom hom
Qhov "Multi Pattern Mode" tso cai rau kev siv lub tshuab hluav taws xob ntawm cov npe ntawm cov qauv ua ntej stimulation electrode. Cov qauv yog siv ib qho tom qab lwm qhov tom qab pib teeb liab. Daim ntawv teev cov qauv yuav raug siv ntau zaus nyob ntawm qhov "Loop" teeb tsa. Tib stimulation mem tes yog siv rau txhua tus qauv.

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CMOS-MEA-CONTROL SOFTWARE

Lub ,,Multi Pattern Mode" tuaj yeem toggled ntawm ,,Enable Stimulator Multi Pattern Mode "check box in ,,Tools" of the ,,Application Settings "dialog. Yog tias qhib tau, lub vaj huam sib luag pom nyob rau sab xis ntawm cov txheej txheem electrode.

Txhua qhov nkag hauv daim ntawv teev npe txhais tau ib tus qauv thiab tuaj yeem muaj ib lossis ntau qhov kev xaiv stimulation electrodes, rau ib lossis ntau lub stimulators, cim ntsuab, xiav, thiab liab. Daim ntawv teev qhia cov kev nkag hauv cov lej nrog cov naj npawb ntawm cov electrodes xaiv rau txhua tus stimulator hauv kab lus. Thaum ib qho kev nkag mus rau hauv daim ntawv tau xaiv cov qauv electrode rau txhua tus stimulators tau pom. Vim muaj kev txwv ntawm lub cim xeeb sab hauv, tus naj npawb ntawm cov npe nkag yog txwv rau 256.

Cov haujlwm ntawm ,,Stimulator Multi Pattern Mode" interface yog raws li hauv qab no:
· Clone: Ntxiv ib qho kev nkag rau hauv cov npe nrog tib tus qauv raws li qhov tam sim no xaiv. · Ntxiv Tshiab: Ntxiv ib qho tshiab nkag mus rau qhov kawg ntawm daim ntawv teev npe nrog tus qauv khoob electrode. · Tshem tawm: Tshem tawm cov kev xaiv tam sim no los ntawm cov npe. · Txuag/Load: Txuag cov npe qauv rau a file los yog load los ntawm a file (*.lmp). Tsuas yog qauv files nrog tib MEA layout tuaj yeem thauj khoom.
Loading overwrite cov npe tam sim no nkag.
· Loop: Txiav txim qhov hloov mus rau tus qauv tom ntej hauv daim ntawv. o muab tua: tsuas yog ib qho qauv siv, kom txog rau thaum qhov tshwm sim tom ntej o 1: tag nrho cov qauv hauv daim ntawv thov ib zaug, ib qho tom qab lwm tus o 2,3: tag nrho cov qauv hauv daim ntawv tau siv ib zaug, tom qab ntawd daim ntawv teev npe rov ua dua ob lossis peb zaug, ntsig txog o infinite: daim ntawv teev cov qauv raug siv kom txog thaum lub stimulation nres
· Rov pib dua: Rov pib dua tus qauv tam sim no rau thawj qhov nkag hauv daim ntawv teev npe.

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CMOS-MEA-CONTROL SOFTWARE
Deactivated/Activated Cov ntau cov qauv yog cia li rub tawm mus rau lub cuab yeej, thaum lub pob ,,Deactivated "yog liab. Nyem rau ntawm lub pob thiab lub cuab yeej yog armed, khawm hloov mus rau ntsuab ,, Ua kom ". Pib lub stimulation ntawm lub ,,Download thiab Start" khawm nyob rau hauv predefined hom.
Yog hais tias ,, Loop "yog muab tua, lub stimulator yuav tsum nres tom qab tus qauv stimulation tau tas mus txog rau thaum pib tshwm sim tom ntej. Tom ntej no electrode qauv nyob rau hauv daim ntawv no yog preselected thiab tau thov los ntawm lub tom ntej no trigger. Yog tias ,, Loop "tau qhib, cov qauv hauv cov npe tau txais kev thov ib leeg tom qab ib leeg (saib saum toj). Yuav tsum muaj tsawg kawg yog ib qho electrode xaiv, txwv tsis pub qhov hloov mus rau tus qauv tom ntej yuav tsis tshwm sim. Yog hais tias lub stimulator nres manually, ces tsis muaj kev hloov mus rau lwm yam electrode qauv tshwm sim. Kev tuav ntawm tus qauv stimulus tsis hloov los ntawm ,,Multi Pattern Mode ", nrog rau ib qho kev zam: Ib qho kev txhawb nqa nrog ,, inifinite "lub voj tsis sib haum nrog ,, Ntau Hom Hom "muaj nuj nqi. Ib tus qauv nrog lub sijhawm tsis kawg yuav txwv tsis pub hloov cov qauv electrode.

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CMOS-MEA-CONTROL SOFTWARE
Stimulus Pattern: Flat Line

Thawj qhov muab primitive yog ib txoj kab ncaj nraim stimulus. Txhawm rau kho lub sijhawm, thov siv lub thawv nce-down: teev, feeb, thib ob, millisecond thiab microsecond. Txhais tus nqi ntawm tus qauv hauv "Amplitude (mV)” up-down box.
Stimulus Pattern: Sine Wave Warning: Negative voltages yuav puas CMOS arrays. Sine nthwv dej yuav tsum tau hloov nrog qhov offset rau qhov zoo ntau! Module lub amplus "PP Amp (mV)”, lub sijhawm “Lub Sijhawm (s), kev ua haujlwm “ua haujlwm” thiab theem “Phase (') ntawm sine yoj qauv ntawm lub thawv nce-down. Teem tus naj npawb ntawm cov voj voog mus rau ntau tshaj ib qho ua rau muaj kev txhawb nqa lub sijhawm "ISI (s)" nce-down box. Tsis tas li ntawd, lub pob "Xub" yuav qhib.

Thaum siv ntau tshaj ib lub voj voog koj tuaj yeem nyem rau ntawm lub cim xub rau kev xaiv ntxiv: Ib qho kev qhia tshwj xeeb uas pom tau los hloov kho amplitude thiab lub sijhawm ntawm sine nthwv dej hauv kev sib raug zoo rau ib leeg. Hauv no example nce amplitude ntawm txhua sine yoj ntawm 16 mV piv rau sine yoj ua ntej. Xaiv tus nqi tsis zoo los ntawm lub thawv nce-down, lub amplitude yuav txo qis piv rau nthwv dej ua ntej. Analogous koj tuaj yeem kho tus nqi ntawm lub sijhawm nyob rau hauv s nyob rau hauv kev sib raug zoo rau yoj ib ntus. Lub qhov rais "Single Pattern" qhia cov sine nthwv dej hauv ib qho kev sib tshooj. Lub qhov rais hauv qab "Ua tiav Stimulation Pattern" qhia txog kev sib xyaw ua ke ntawm txhua qhov kev txhawb nqa pulses.

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CMOS-MEA-CONTROL SOFTWARE
Tus Qauv Txheej Txheem: Ramp Qhov sib npaug ntawm biphasic tam sim no mem tes yog daim duab peb sab voltaguaamp. Txog 9 qhov stimulation pads (3 × 3) yam tsawg kawg yog xav tau rau kev txhawb nqa zoo (tseem siv tau rau retina, tsis muaj ntaub ntawv tseem ceeb rau kab lis kev cai ntawm tes). Nyob rau hauv sib piv rau ib txwm MEAs, nyob sib ze cov ntaubntawv povthawj siv sensors feem ntau yuav tsis saturate thaum lub sij hawm stimulation, yog li cov ntaubntawv povthawj siv nyob ze ze rau qhov chaw stimulation yog ua tau. Module lub amplitude "Amplitude (mV)” thiab lub sijhawm “Lub Sijhawm (s)” ntawm ramp mem tes ntawm cov thawv nce-down. Txhawm rau kho qhov steepness ntawm caj npab ntawm lub mem tes, thov siv peb lub thawv nce-down rau qhov nce toj, toj siab thiab nqis caj npab ywj siab ntawm ib leeg. Teem tus naj npawb ntawm cov voj voog mus rau ntau tshaj ib qho ua rau muaj kev txhawb nqa lub sijhawm "ISI (s)" nce-down box. Tsis tas li ntawd, lub pob "Xub" yuav qhib.

Thaum siv ntau tshaj ib lub voj voog koj tuaj yeem nyem rau ntawm lub cim xub rau kev xaiv ntxiv: Ib qho kev qhia tshwj xeeb uas pom tau los hloov kho amplitude thiab lub sijhawm ntawm ramp mem tes hauv kev sib raug zoo rau ib leeg. Lub qhov rais "Single Pattern" qhia tus ramp mem tes nyob rau hauv ib tug overlay zajlus. Lub qhov rais hauv qab "Ua tiav Stimulation Pattern" qhia txog kev sib xyaw ua ke ntawm txhua qhov kev txhawb nqa pulses.
Stimulus Pattern: Biological Pulse ntawm ASCII Import Warning: Negative voltages yuav puas CMOS arrays. Sine nthwv dej yuav tsum tau hloov nrog qhov offset rau qhov zoo ntau! Qhov tseem ceeb: qhov kev daws teeb meem ntawm stimulator yog 10 µs nyob rau hauv lub sij hawm axis thiab 105 µV nyob rau hauv voltage. Lub tshuab hluav taws xob tsis muaj peev xwm tso tawm cov pulses luv dua 10 µs lossis qis dua 105 µV, yog li cov pulses no hla! Yog tias koj nyiam siv, example, ib lub teeb liab lom raws li stimulus mem tes los yog koj xav tsim ib tug arbitrary qauv, koj muaj peev xwm import cov cim los ntawm txhaj rau "Ntshuam" khawm. Cov imported file yuav tsum tau ua kom muaj cov qauv hauv qab no:

Sijhawmamp Voltage Tus Nqi Sijhawmamp Voltage Tus Nqi Sijhawmamp Voltage Tus nqi…
Unit ntawm lub sij hawmamp yog "µs" thiab lub sij hawm tus nqi yuav tsum nyob rau hauv ascending order. Unit ntawm voltage tus nqi yog "µV". Cov units tsis yog ib feem ntawm cov file. CMOS-MEA-Control software lees txais cov lej tsuas yog thiab commas, tabulators lossis qhov chaw cais lub sijhawmamp thiab voltage nqi. Thov tshem tawm cov header ua tau, zam cov kab blanc thiab siv cov kab tshiab rau txhua tus khub. Txuag tus file tsis txuas ntxiv lossis nrog ib qho txuas ntxiv "*.dat", rau example, los yog “*.csv”, thaum siv lub comma sib cais file.

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CMOS-MEA-CONTROL SOFTWARE
Yog tias import file tsis ua raws li qhov yuav tsum tau ua, cov lus yuam kev tau qhia. Stimulus Pattern: Biological Pulse Example: Ntshuam ntawm cov hauv qab no file Raws li stimulation qauv:

Nco tseg: Vim tias txoj kab nqes ntawm lub teeb liab txiav txim siab lub zog ntawm qhov induced tam sim no, thov ntxiv ramps mus rau qhov pib thiab nres ntawm cov teeb liab lom neeg kom tsis txhob muaj cov khoom siv dag zog.

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CMOS-MEA-CONTROL SOFTWARE
Stimulation Pattern: Loop

Muab cov qauv kev txhawb nqa ntawm qhov "Loop" primitive thiab cov qauv tuaj yeem rov ua dua ntau zaus raws li xav tau. Thov xaiv tus naj npawb ntawm looping "Cycles" los ntawm lub thawv nce-down. Lub "Loop" primitive tsis txav tau nyob rau hauv ib theem ntawm cov qauv stimulus. Muab tso rau hauv lub thawv thiab pib dua los hloov txoj haujlwm ntawm "Loop" qauv. Nws yog ua tau los tsim convoluted qauv.

Txhawm rau tso qhov tseem ceeb ntawm "Start Loop"

thiab "Stop Loop"

bracket, thov luag thiab tso cov thawj thawj zaug

ua ntej los yog tom qab lub brackets thiab ces mus rau sab laug los yog mus rau sab xis tshaj ib qho ntawm cov bracket nyees khawm ntawm lawv.

Tool Bar
Cov kev teeb tsa hauv qhov cuab yeej bar no cuam tshuam rau qhov ua tiav ntawm cov qauv kev txhawb nqa. Module qhov "Amplitude (%)" thiab "Offset (mV)" ntawm cov qauv ua tiav ntawm lub thawv nce-down.

Nias lub khawm "Loop" ua rau koj rov ua dua cov qauv kev txhawb zog uas tau hais tseg. Thauj cov qauv tsim tawm dhau los, txuag lossis tshem tawm cov qauv. Thaum siv cov cim cim cim, koj yuav tsum txhais qhov twg ntawm "Marker Port" (digital out) yuav tsum qhia cov cim TTL sib raug. Xaiv ib qho ntawm cov cim chaw nres nkoj los ntawm cov ntawv qhia zaub mov nco thiab txuas TTL teeb liab qhov chaw mus rau qhov chaw nres nkoj.

Pib qhov stimulation manually.
Rub tawm thiab pib tsim cov qauv txhawb nqa ntawm lub "Download" icon. Yog tias tsis tsim nyog rub tawm, lub icon tsis muaj. Txoj kev no koj muaj kev tawm tswv yim kho qhov muag rau kev rub tawm.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS

CMOS-MEA-TOOLS Taw Qhia Lub software rau tswj CMOS-MEA5000-System suav nrog ob ntu, CMOS-MEA-Control rau kev kaw hauv online thiab CMOS-MEATools rau kev tshuaj xyuas offline. Nrog Multi Channel DataManager software koj tuaj yeem xa cov ntaub ntawv mus rau peb daim ntawv thov. Qhov rai Main

Lub qhov rais default ntawm cov zaub mov pib tau muab faib ua ob ntu tseem ceeb: "Control Window" thiab "Data Display or Settings Window". Sab laug muaj cov haujlwm tswj: "Tswj Tsob Ntoo" view thiab lub qhov rais "Activity Summary". Saib lub sab cev nqaij daim tawvview ntawm qhov ua tiav file nyob rau hauv ob nplooj ntawv tab: Cov "Xaiv Kev Ua Si" tab qhia tag nrho cov spikes, hauv "Kev tshwm sim" tab cov txheej xwm stimulator tau teev tseg. Lub qhov rais thib peb qhia tau hais tias "Spatial Distribution". Qhov sab xis suav nrog cov ntaub ntawv qhia lossis teeb tsa lub qhov rais thiab muaj plaub tab hauv kev sib raug zoo rau cov cuab yeej teev hauv tsob ntoo view: "Filter Pipeline", "Raw Data Explorer", "Spike Explorer" thiab "STA Explorer". Ob lub qhov rais yog framed los ntawm cov ntawv qhia zaub mov thiab cov cuab yeej bar hauv header thiab cov file cov ntaub ntawv hauv qab no. File
Ntawv qhia zaub mov rau import cov ntaub ntawv nyoos rau kev tshuaj xyuas: “Qhib Raw Data File"nrog rau qhov txuas ntxiv "*.cmcr" tsim nrog "CMOS-MEA-Control" software rau kev tshuaj xyuas thiab mus rau "Tawm" qhov kev pab cuam. Ntawv qhia zaub mov rau "Load Result File"nrog rau qhov txuas ntxiv "*.cmtr" rau kev rov ua dua tshiab thiab txuag cov txiaj ntsig files. Ntawv qhia zaub mov rau "Tawm" qhov program.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
Ua raws file Cov hom ntawv muaj rau CMOS-MEA-Tools:
· *.cmcr: CMOS-MEA-Control Raw Data · *.cmct: CMOS-MEA-Control Templates · *.cmtr: CMOS-MEA-Tools Results · *.cmtt: CMOS-MEA-Tools Templates · *.cmte: CMOS-MEA-Tools Export
Nco tseg: Doubleclick a "CMOS-MEA-Control Raw Data" lossis "CMOS-MEA-Tools Results" file qhib nws ncaj qha los ntawm daim nplaub tshev yam tsis tau pib CMOS-MEA-Tools software ua ntej. Txheeb xyuas
Ntawv qhia zaub mov rau kev tsom xam ntawm cov imported file. Pib qhov kev tshuaj ntsuam nrog rau "Process Segment" hais kom ua rau ib ntu thiab nrog rau "Process File” hais kom ua tiav file. Nyem "Batch Analysis" txhawm rau txheeb xyuas ntau dua ib qho file hauv ib pawg. Chaw
Cov ntawv qhia zaub mov los teeb "Application Settings" thiab thauj khoom thiab txuag cov qauv. Cov ntawv qhia zaub mov qhib "Labbook" dialog.

Dialog nrog peb tab rau cov ntaub ntawv dav dav txog qhov kev sim. Sau cov ntaub ntawv xa mus rau koj qhov kev sim hauv "Study" tab, "General" sau ntawv txog tus kws tshawb fawb thiab lub koom haum thiab tags thiab cov ntawv dawb hauv "Tags thiab Notes" tab. Qhov "Labbook Settings" yuav muab tso rau hauv "CMOS-MEA-Tools" tshwm sim file "*.cmtr". Pab
Cov ntawv qhia zaub mov qhib "Kev Pabcuam Online" thiab mus rau "Check for Update" thiab saib cov ntaub ntawv hauv "Txog".

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CMOS-MEA-TOOLS
Qhov kev xaiv "Kuaj rau Hloov Kho" muab ceev ceev rau MCS webqhov chaw.

Yog tias CMOS-MEA-Tools software tsis hloov tshiab, nyem lub pob "Mus saib Website" thiab rub tawm cov software tshiab tshaj plaws lossis siv lub pob "Download" txhawm rau rub tawm qhov hloov tshiab ncaj qha.
Nyem "Txog" los qhib lub dialog.

Cov lus sib tham qhia cov ntaub ntawv yooj yim txog CMOS-MEA-Tools software version. Nco tseg: Thov nco ntsoov tias cov ntaub ntawv ntawm qhov kev sib tham no yog qhov tsim nyog thaum muaj kev txhawb nqa!
Toolbar

Qhib cov ntaub ntawv nyoos File
Nyem "Open Raw Data File"hauv"File” zaub mov los yog nyem rau ntawm “Qhib Cov Ntaub Ntawv Raw” icon Cov ntaub ntawv File” tshwm.

hauv toolbar. Lub dialog “Xaiv CMOS-MEA-Raw

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
Nyem "Open Raw Data File” qhib lub dialog “Xaiv CMOS-MEA Raw Data File”.

Xaiv txoj kev nkag. Qhov kev zov me nyuam tab tom luam theej duab rau kev txheeb xyuas files: “*.cmcr” CMOS-MEA-Control Raw data and “*.cmtr” CMOS-MEA-Tools Result files. Qhov kev tshawb nrhiav no tuaj yeem siv sijhawm me ntsis, thov saib lub bar hauv "Scanning Input Folder" dialog.

Muaj files tau teev nyob rau sab sauv ntawm lub dialog. Xaiv cov ntaub ntawv file los ntawm cov npe thiab nrhiav cov ntaub ntawv hais txog "File Lub npe, Hnub tim, Duration, Txheej xwm, Loj, SW Version, Chip ID thiab Chip Info". Ib tug xaiv file yog highlighted nyob rau hauv xiav thiab cov nkag ntawm lub lab phau ntawv ntawm lub feem file yog qhia nyob rau hauv qis ib feem ntawm lub dialog.

Qhib a file nrog ob npaug nias rau ntawm qhov xaiv file los yog nias lub "Qhib File"Nrhiav recursively" nyob rau hauv lub headline ntawm lub dialog, yog hais tias tsim nyog.

khawm. "Refresh" daim ntawv los yog nyem lub thawv kos

Qhib qhov tshwm sim File

Nyem qhov "Load Result" khawm kom qhib qhov tshwm sim file

thiab "Xaiv CMOS-MEA-Tsev tau File”dialog opens. Kev tshwm sim files muaj

extension "*.cmtr". Reanalyze qhov tshwm sim file thiab txuag nws dua nyob rau hauv lub npe tshiab.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS

Batch Analysis
Qhov no feature tso cai rau txheeb xyuas ib tug xov tooj ntawm files tag nrho ib zaug, uas yuav pab tau nyob rau hauv cov ntaub ntawv ntawm ib tug loj npaum li cas ntawm cov ntaub ntawv files.
Ua ntau ntau files hauv ib qho kev khiav koj tuaj yeem siv "Batch Analysis" built-in feature ntawm CMOS-MEA-Tools. Cov ntaub ntawv nyoos xaiv files tau ua tiav ib qho tom qab lwm qhov siv cov txheej txheem ua ntej ntawm cov cuab yeej thiab kev teeb tsa. Rau txhua cov ntaub ntawv raw file, cais qhov tshwm sim yog khaws cia rau ntawm qhov chaw ua ntej. Qhov no tso cai rau khiav ib lub sij hawm siv tsom xam ntawm ntau files tsis muaj kev saib xyuas tas li.

Nyem "Batch Analysis"

qhib lub dialog rau xaiv cov files rau ib pawg.

Thov xaiv "Input Path" hauv lub header. Txoj kev nkag yog cov hauv paus directory siv rau kev tshawb nrhiav cov ntaub ntawv nyoos files. Yog tias xaiv lub thawv "Search recursively", tag nrho cov subfolders ntawm txoj kev nkag mus tau tshawb nrhiav. Cov files ntawm txhua daim ntawv tais ceev tseg tau qhia ua ib pawg. Xaiv tag nrho files los ntawm nias lub pob "Xaiv / Tshem Tawm Tag Nrho" lossis toggle xaiv ntawm a file los ntawm nias lub pob "Xaiv / Deselect" nyob rau hauv thawj kab nyob rau hauv pem hauv ntej ntawm lub file npe. Txoj kev tso zis txhais cov ntaub ntawv hauv paus rau qhov tshwm sim files. Yog cov ntaub ntawv raw files nyob rau hauv sib txawv folders, tib lub folders qauv yog generated rau qhov tshwm sim files. Pib "Batch Analysis" los ntawm nias lub pob "Start". Cov xaiv files yog ua tiav los ntawm sab saum toj mus rau hauv qab thiab cov xwm txheej ntawm cov txheej txheem yog depicted los ntawm kev kawm tuav. Kev tsom xam batch tuaj yeem nres los ntawm nias lub pob "Stop".
Kev soj ntsuam pom zoo ua haujlwm
1. Cov ntsiab lus 2. Tshawb 3. txheej txheem File 4. Batch Analysis Thaum thauj cov ntaub ntawv raw file, lub software yuav pib nyob rau hauv "Explore" hom. Qhov no tso cai rau tshuaj xyuas ib feem ntawm cov ntaub ntawv nyoos hauv "Raw Data Explorer". Ib tshajview dhau qhov kev ua si ntawm lub cev thiab qhov chaw spatial spiking hauv koj cov ntaub ntawv teev tuaj yeem tau txais nrog "Cov Cuab Yeej Ntsuas". Dhau li ntawm kev pom cov ntaub ntawv nyoos, kev siv lub ntsiab ntawm "Xav" hom yog sim thiab hloov cov chaw sib txawv, xws li lim lossis cov ntsuas ntsuas qhov ntsuas. Thaum koj txaus siab, koj tuaj yeem txheeb xyuas tag nrho file nrog cov kev teeb tsa no siv "Process File”. Thaum kawg, "Batch Analysis" hom tuaj yeem siv los txheeb xyuas ntau cov ntaub ntawv nyoos files nrog ib lossis ntau qhov kev txheeb xyuas qhov ntsuas.

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CMOS-MEA-TOOLS
Pom zoo Workflow rau cov txiaj ntsig Files Thaum thauj khoom tshwm sim file, lub software yuav pib hauv "Process File” hom thiab tso saib cov txiaj ntsig muaj nyob hauv file. Raws li cov ntaub ntawv raw files, koj tuaj yeem hloov mus rau "Xaiv" hom los ntsuas qhov ntsuas tshiab ntawm cov ntaub ntawv thiab mus rau view cov ntaub ntawv raw sensor. Txawm li cas los xij, thov nco ntsoov tias kev hloov mus rau "Xav" hom yuav muab pov tseg cov txiaj ntsig tam sim no pom hauv CMOS-MEA-Tools software. Nyem "Process File” yuav reanalyze cov ntaub ntawv raw file txuam nrog qhov tshwm sim file siv cov txheej txheem tam sim no. Qhov kev nce qib ntawm cov ntaub ntawv tsom xam tau tshwm sim tam sim hauv cov cuab yeej cuab tam view. Cov ntaub ntawv ntawm qhov tseeb txheeb xyuas file muaj nyob rau hauv lub footer.

1. Cov ntsiab lus

Cov cuab yeej "Summary" muab ib qho kev ntxhib los mos ntawm lub cev thiab sab nraudview ntawm qhov kev ua si hauv file los ntawm kev txheeb xyuas tag nrho file nrog ib tug yooj yim spike nrhiav kom tau txoj kev. Nws tuaj yeem muaj txiaj ntsig zoo los txiav txim siab thaj tsam ntawm kev txaus siab rau kev txheeb xyuas ntxaws ntxiv nrog "Xav" hom.

Nias lub pob "Qhib Chaw Dialog"

txhawm rau txhim kho qhov ntsuas qhov ntsuas qhov ntsuas thiab qhov pom kev teeb tsa.

Kho cov chaw rau kev tsom xam. Tej zaum nws yuav tsim nyog ua si me ntsis nrog cov chaw kom txog thaum koj pom qhov chaw zoo tshaj plaws.
2. Tshawb xyuas hom
Hom "Explore" ua haujlwm los ntawm kev thauj khoom ib ntu ntawm cov ntaub ntawv nyoos rau hauv lub cim xeeb uas tuaj yeem pom hauv "Raw Data Explorer" lossis siv los ntsuas cov ntsuas sib txawv.
Lub sij hawm thauj khoom ntu yog qhia hauv lub teeb xiav nyob rau hauv "Xaiv Kev Ua Si" tab. Nws lub sijhawm yog txwv rau qhov siab tshaj plaws ntawm 3 vib nas this kom tsis pub dhau lub cim xeeb muaj peev xwm. Yog tias lwm cov cuab yeej tshuaj ntsuam tau ua haujlwm, lawv yuav txheeb xyuas cov ntaub ntawv thauj khoom ntu ib yam. Vim tias khiav ib qho kev soj ntsuam tag nrho ntawm cov ntaub ntawv thauj khoom yuav siv sij hawm ntev, "Spike Sorter" tsuas yog ua qhov ROI nrhiav pom hauv "Xav" hom.
Thov siv qhov "Process File”los yog “Batch Analysis” hom los khiav ib qho kev ntsuam xyuas tag nrho.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
3. Txheej txheem File Tom qab teeb tsa cov ntsuas ntsuas, piv txwv liamptom qab optimizing lawv ntawm "Xav" hom, nias "Process File” txhawm rau txheeb xyuas qhov ua tiav file. Rau kev tsom xam, cov ntaub ntawv yog loaded nyob rau hauv luv luv ntu thiab ua tiav los ntawm tag nrho cov active tsom xam cov cuab yeej. Cov kev ntsuam xyuas kev ua tau zoo yog pom nyob rau hauv "Xaiv Kev Ua Haujlwm" view. Yog tias "Spike Sorter" thiab / lossis "STA Explorer" cov cuab yeej ua haujlwm, nws yuav siv sijhawm ntau dua ib qho dhau los ntawm file kom tiav qhov kev txheeb xyuas. Cov "Raw Data Explorer" raug kaw hauv "Process File” hom, vim tias nws yuav tsis tuaj yeem thauj cov ntaub ntawv nyoos tag nrho file nyob rau hauv lub cim xeeb rau visualization. Lub sij hawm nws yuav siv sij hawm rau ib qho kev ntsuam xyuas tiav ntawm a file nyob ntawm qhov ntev ntawm cov ntaubntawv povthawj siv thiab kev ua haujlwm ntawm lub computer. Thov nco ntsoov tias kev khiav qhov kev txheeb xyuas qhov sib txawv tuaj yeem siv sijhawm ntau heev.
4. Batch Analysis Hom "Batch Analysis" tuaj yeem siv los txheeb xyuas ib lossis ntau cov ntaub ntawv nyoos files nrog kev txheeb xyuas sib txawv. Thov nyeem tshooj “Batch Analysis” saum toj no kom paub ntau ntxiv. Lub sijhawm nws siv rau kev tshuaj xyuas batch yog nyob ntawm qhov ntev ntawm cov kaw lus thiab kev ua haujlwm ntawm lub computer. Thov nco ntsoov tias kev khiav qhov kev txheeb xyuas qhov sib txawv tuaj yeem siv sijhawm ntau heev.
Tswj Qhov rai

Qhov "Control Section" nyob rau sab laug ntawm cov zaub muaj peb lub qhov rais: "Tsev Tsob Ntoo" ntawm cov cuab yeej muaj nyob saum toj no, lub qhov rais "Txoj Haujlwm Summary" lub sijhawm thiab qhov chaw faib tawm "Cov ntsiab lus" hauv qab no.
Toolbar

Nyem rau "Tsim cov ntsiab lus" icon xam.

los yog siv cov ntawv qhia "Tsim" rau kev thauj cov ntaub ntawv hauv RAM. Tam sim no cov ntaub ntawv nyoos muaj rau

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS

Nyem rau "Qhib Chaw Dialog"

thiab cov dialog hauv qab no tshwm. Kho cov chaw rau kev tsom xam.

Thov txhais qhov pib. Xaiv qhov "Thiab Pib" los ntawm cov ntawv qhia zaub mov nco, "Zoo", "Tsis zoo" lossis "Tsis Muaj". Xaiv qhov "Threshold" tus nqi thiab "Nrhiav Tuag Sijhawm" hauv ms nrog lub thawv nce-down. Xaiv "Bin Size" hauv ms nrog lub npov nce-down. Xaiv cov xim ntawm "Color Map". Txhawm rau hloov cov xim, thov nyem lub thawv "Invert". Tsob Ntoo Tsob Ntoo
"Teeb View” qhia tag nrho cov cuab yeej muaj thiab cov xwm txheej tiag tiag. Nyem ib qho ntawm cov cuab yeej hauv tsob ntoo view kho tej qhov parameter. Qhib nplooj ntawv tabbed ntsig txog qhov ntsuas ntawm qhov "Cov Ntaub Ntawv Qhia lossis Chaw" qhov rai, piv txwv liample "Spike Sorting" raws li qhia hauv lub screenshot hauv qab no. Thov nyeem tshooj ,,Spike Sorter Tool” ntawm nplooj ntawv Fehler! Textmarke nicht definiert. rau cov ncauj lus kom ntxaws.

Thaum nias lub pob "Xaiv cov ntaub ntawv ntu".

, tag nrho cov ntaub ntawv xaiv nyob rau hauv cov ntaub ntawv tam sim no ntu ntawm "Kev Ua Haujlwm Summary"

qhov rai raug tshuaj xyuas. Los yog nyem "Process File” khawm

pib tsom xam kom tiav file. Thaum lub sij hawm tsom xam cov txheej txheem koj

tuaj yeem soj ntsuam qhov kev nce qib qhia los ntawm cov ntaub ntawv ntws, tseem ceeb hauv xiav xiav.

Tom qab kev soj ntsuam ua tiav, cov cuab yeej koom nrog qhia pom cov cim kos.

Nyob rau hauv rooj plaub uas muaj cov ntaub ntawv loj heev nws yuav tsis tuaj yeem thauj khoom tiav file rau hauv RAM thiab kev tshuaj xyuas yuav tsum tau rov ua dua lossis yog tias qhov kev soj ntsuam raug nres lossis cuam tshuam rau ib qho laj thawj, cov ntsuas ntsuas tsis muaj qhov cim kos. Li no nws yog ib qho yooj yim los tswj cov kev txheeb xyuas.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
Cov ntsiab lus ua haujlwm

Ob lub qhov rais tso saib cov ntsiab lus ntawm qhov kuaj pom spikes ntawm qhov ua tiav file. Daim duab saum toj no qhia tau hais tias lub cev nqaij daim tawv dhauview, daim ntawv qhia hauv qab no qhia tau hais tias spatial tshajview.
Tshawb Kev Ua Haujlwm

Lub sij hawm dhau musview qhia cov naj npawb ntawm cov kab mob kuaj pom ib qho ntxiv los ntawm txhua txoj hauv kev rau lub sijhawm ua tiav ntawm qhov file. Yog li koj tuaj yeem pom lub sijhawm ua haujlwm tshaj plaws thaum lub sijhawm kaw cia ib pliag.
Xaiv lub sijhawm ncua sij hawm koj xav txheeb xyuas hauv "Lub Sijhawm" daim duab. Nyem rau ntawm lub cim "Xav txog lub sijhawm txhais tes taw qhia" txhawm rau teeb tsa lub sijhawm ncua ntawm tus kheej.
Nyem lub xiav tuav thiab txav lawv ntawm luag thiab poob mus rau qhov xav tau txoj haujlwm. Lub sijhawm nruab nrab ntawm cov kab xiav, uas yuav suav nrog yog qhov tseem ceeb hauv xiav. Cov spikes rau ib qhov yog suav nrog lub sijhawm. Zoom rau hauv cov ntaub ntawv los ntawm kev txav tus nas los ntawm sab laug mus rau sab xis thiab kom zoom tawm ntawm sab xis mus rau sab laug.
Cov xwm txheej

Nyem rau ntawm "Kev Ua Haujlwm Summary" qhov rai nplooj ntawv tab "Cov xwm txheej" txhawm rau sau tag nrho cov xwm txheej digital los ntawm stimulator, kaw rau hauv qhov ntawd file nrog rau lub sijhawm stamp hauv qhov ntsuas. Cov txheej xwm stimulator sib txawv yog xim coded. Xaiv ib lossis ntau tshaj ib qho kev tshwm sim uas yog qhov tseem ceeb hauv ntsuab thiab siv cov xwm txheej no rau kev taug kev los ntawm qhov file nyob rau hauv lub tab "Xaiv Kev Ua Haujlwm" tab.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS

Nyem "Xaiv xwm txheej raws li"

thiab cov cuab yeej bar ib sab yog tam sim no muaj.

Cov txheej xwm xaiv tau pom xim coded hauv qab cov ntaub ntawv. Dhia los ntawm cov pob xub "Mus mus rau qhov tshwm sim tom ntej / yav dhau los" los ntawm qhov xwm txheej mus rau qhov xwm txheej, ntu sijhawm tiag tiag qhia los ntawm cov kab xiav li niaj zaus. Thov txhais lub sij hawm ntu "Pre" thiab "Post" qhov kev tshwm sim digital hauv ms nrog lub thawv nce-down. Thaum dhia los ntawm qhov xwm txheej mus rau qhov xwm txheej cov ntaub ntawv qhia hauv "Raw Data Explorer" qhov rai ntawm sab xis yog hloov kho tam sim ntawd.

Nyob rau yav tom ntej versions ntawm no software, qhov kev tshwm sim raws li kev tsom xam yuav muaj. Thov xyuas MCS web site rau xov xwm.

Spatial Distribution
Lub spatial tshajview qhia tus naj npawb ntawm cov kab mob uas tau pom ib channel rau qhov ua tiav file xim coded nyob rau hauv daim ntawv qhia. Nws yog ib qho yooj yim los soj ntsuam cov chaw nquag tshaj plaws hauv cov array.

Cov lej hauv cov kab ke yog cov kab ke ntawm tus nas nyob rau hauv daim ntawv qhia dav dav.
Kho qhov "Range" nrog lub thawv nce-down. Qhov ntau yog nyob ntawm tus naj npawb ntawm spikes ib sensor. Qhov qis dua, qhov siab dua qhov kev faib tawm ntawm cov sensors nrog spikes ntxiv mus rau "Range" tus nqi. Cov xim ntawm daim duab qhia chaw qhia cov chaw ua haujlwm ntxiv.

Txhais cov xim ntawm daim ntawv qhia nyob rau hauv "Summary Tool Settings"

dialog.

Cov ntaub ntawv tso saib thiab chaw qhov rais Qhov rai no muaj tsib nplooj ntawv tab nyob rau hauv kev sib raug zoo rau cov twj paj nruag nyob rau hauv "Tswj Tsob Ntoo". Lim

Peb hom lim tau muab: "Lub Sijhawm Lim" xws li "Band-Pass", "High-Pass", "Notch" thiab "Low-Pass", "Space Filter" thiab "Artefact Filter". Nyem qhov "Filter" icon nyob rau hauv "Tswj Tsob Ntoo" thiab cov lim teeb icons muaj.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
Filter Pipeline Tsim cov raj xa dej hauv qhov "Filter" qhov rai tswj. Nyem rau ntawm lub cim lim xav tau thiab txav mus los ntawm kev luag thiab poob rau hauv kab ntawv header.
Cov ntxaij lim dej uas tau xaiv yog tsom rau hauv xiav thiab cov kev teeb tsa tsis zoo lossis cov lus piav qhia muaj nyob rau hauv lub qhov rais. Saib ua ntejview ntawm "Raw" thiab "Filtered" cov ntaub ntawv hauv cov qhov rai me hauv qab no, yog tias muaj.

Lim Toolbar

Nyem rau "Cov Ntaub Ntawv Txheej Txheem" icon thov nyem rau hauv.

rau thauj cov ntaub ntawv xaiv hauv RAM. Kom tshem tawm cov lim kab,

Khaws cov hlua lim thiab qhib nws tom qab.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
Raw Data Explorer Load ib cov ntaub ntawv raw file thiab nyem "Explore" khawm hauv lub cuab yeej bar ntawm cov ntawv qhia zaub mov tseem ceeb.
Xaiv lub "Raw Data Explorer" tab nyob rau hauv "Data Display lossis Chaw" qhov rai.

Lub "Raw Data Explorer" tab qhia txog kev ua haujlwm loj: Qhov "Tshajview" rau tag nrho cov electrodes saum toj no thiab "Single View"ntawm ib qho electrode hauv qab no. Zoom rau hauv cov ncauj lus kom ntxaws view nyob rau hauv "ROI cheeb tsam ntawm kev txaus siab". Cov "Raw Data Explorer" rov ua haujlwm ntawm cov ntaub ntawv nyoos file zoo li hauv dhauview raws li tau piav qhia hauv ib cheeb tsam ntawm kev txaus siab lossis hauv ib leeg view. Qhov "Kev Ua Haujlwm" qhov rai qhia voltage tus nqi ib lub sij hawm rau hauv raws li ib tug cuav xim duab. Spikes tshwm li xiav lossis liab dots, feem ntau ntawm ob peb tus neeg nyob sib ze pixel hauv daim ntawv qhia ib txhij.
Raw Data Explorer Tool Bar

Txhais qhov "Frame" rau cov ntaub ntawv sample nrog lub up-down box. Nyem rau "Pib Rov Qab" thiab cov "Pib Taw Qhia" khawm kom rov ua cov ntaub ntawv ib kauj ruam hauv txhua qhov kev taw qhia. Rau replaying cov ntaub ntawv tsis tu ncua, nyem qhov "Start / Pause Movie" khawm. Txhawm rau "Slow Down" qhov nrawm ntawm cov ntaub ntawv yeeb yaj kiab, thov siv lub npov nce-down. Qhov siab dua tus lej xaiv qhov qeeb qeeb ntawm cov yeeb yaj kiab yuav khiav, vim tus lej nkag

yuav muab ntxiv rau hauv ms tom qab txhua tus ncej. Teem qhov ntau ntawm cov yeeb yaj kiab nrog cov tuav thiab voj cov yeeb yaj kiab. Arm tus yees duab kaw nrog lub

Nyem "Hloov Video" khawm

thiab lub npe thiab txuag cov yees duab hauv "*.mp4" hom. Hloov chaw video los ntawm txhaj rau "Qhib Chaw Dialog"

khawm

hauv "Video Recorder Settings" dialog.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS

Xaiv qhov "Kev daws teeb meem" thiab "Txuas Tus Nqi" rau cov yees duab los ntawm cov ntawv qhia zaub mov nco. Qhib lub "Include Timestamp” kos lub thawv, yog tias tsim nyog.

Pib lossis tso tseg cov yeeb yaj kiab nrog "Start / Pause Movie"

khawm. Qhib cov yees duab nrog ib tug media player.

Ib leeg View
Tom qab nias lub pob "Teeb Movie Range", ob lub xiav sliders tshwm nyob rau hauv "Single View”hauv qab no. Txav lawv nrog tus nas ntawm luag thiab poob los txhais lub sijhawm ntu koj xav txheeb xyuas hauv cov yeeb yaj kiab. Lub bar ntsuab qhia txog txoj haujlwm tiag tiag ntawm "Kev Ua Haujlwm" zaub.

Thov mus saib tshooj “Cov Ntawd Software General” rau kev kho cov duab.
Lub cuab yeej bar hauv qab "Kev Ua Haujlwm" qhov rai qhia qhov chaw ua haujlwm ntawm tus cursor txoj haujlwm ntawm sab laug. Txav mus kom tiav ROI nrog rau sab laug nas khawm thaum lub cim ntawm ib txhais tes tshwm. Hloov qhov loj ntawm ROI thaum lub cim xub tshwm. Los yog nias lub pob "Tsim ROI".
qhib lub dialog hauv qab no. Yog tias muaj ntau tshaj ib qho ROI, tus cursor tiag tiag yuav raug kho.

Kho qhov "Txoj Haujlwm (X / Y)" thiab "Qhov Loj (W / H) ntawm ROI nrog lub thawv nce-down. Xaiv cov xim ntawm tus ntoo khaub lig plaub hau los ntawm cov xim teev, yog tias lub thawv "Show Cross Hairs" tau qhib.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
Xaiv qhov "Threshold" rau kev kuaj pom qhov dav thiab "Range" los ntawm cov thawv nce-down. Chav tsev tsawg tshaj plaws ntawm qhov ntau yog "nV".
Nyem qhov "Setting" khawm rau daim ntawv qhia xim nyob rau hauv "Kev Ua Haujlwm" qhov rai.
Xaiv cov xim rau daim ntawv qhia los ntawm cov ntawv qhia zaub mov. Txhawm rau "Invert" cov xim qhib lub thawv kos. Zoom Buttons nyob rau hauv "Kev nthuav dav View” thiab “Single View”Windows
Nyem qhov "Kho kom teeb liab Min/Max" khawm. Qhov scaling ntawm y-axis yog teem rau qhov tsawg kawg nkaus thiab siab tshaj plaws ntawm tag nrho cov pom sampnyob rau hauv lub channel.
Nyem "Zoom" khawm. Zooming nyob rau hauv txiav qhov scaling ntawm lub axis nyob rau hauv ib nrab thiab zooming tawm doubles lub scaling. Export cov ntaub ntawv los ntawm "Single View” as duab (“*.bmp”, “*.jpg”, “*png”) lossis as ASCII hauv “*.csv” format. Spike Explorer Qhov rai Xaiv qhov "Spike Explorer" tab hauv "Cov Ntaub Ntawv Qhia lossis Chaw" qhov rai. Siv lub "Spike Explorer" rau lub ntsiab lus ntawm qhov spike parameter thiab pom qhov pom ntawm cov spikes pom.

Lub "Spike Explorer" lub qhov rais tseem ceeb tau muab faib ua peb ntu, kev ua haujlwm loj heevview, thiab ob lub qhov rais rau thaj tsam ntawm kev txaus siab ROIs. Ib cheeb tsam ntawm kev txaus siab qhia txog cov phiaj xwm sib tshooj, lwm qhov kev faib tawm ntawm lub cev ntawm spikes. Xaiv los ntawm "Spike Activity Overview” nyob rau sab laug ib lossis ntau thaj tsam ntawm kev txaus siab. Cov phiaj xwm sib tshooj ntawm cov pob txha pom tau tshwm sim nyob rau sab xis. Lub qhov rais hauv qab no qhia txog kev faib tawm ntawm lub cev hauv lub sijhawm stamps rau txhua tus ROI xaiv.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
Spike Explorer Toolbar

Nyem rau "Process Data" icon

Txhawm rau pib qhov kev tshuaj ntsuam los ntawm kev ua cov ntaub ntawv thauj khoom hauv RAM.

Nyem qhov "Export Data" khawm

. Xaiv seb puas yuav xa cov spikes "Ua tiav" lossis "Xaiv" ntawm cov ntawv qhia zaub mov. Cov ntaub ntawv tuaj yeem yog

exported hauv "HDF5" lossis "CSV" hom.

Nyem qhov "Spike Explorer Chaw" khawm Tshawb Pom" qhov rais.

txhawm rau txhais qhov "Spike Detection" hauv "Nrhiav", "Spike Cutout" thiab "Kev Ua Haujlwm Peak

Spike Detection
Thov teeb qhov "Nrhiav" parameter los ntawm lub qhov rais sab saud: "Thiab qhov pib" thiab "Suab nrov ntsuas" los ntawm cov ntawv qhia zaub mov nco-down, "Threshold" thiab "Nrhiav Tuag Sijhawm" hauv ms los ntawm lub thawv nce-down. Txhais qhov "Spike Cutout" parameter los ntawm lub qhov rais hauv qab: "Waveform Alignment" los ntawm lub npov nco-down, "Pre thiab Post Intervals" los ntawm cov thawv nce-down.
Kev Ua Haujlwm Peaks Detection
Nyem lub checkbox "Auto Extract thaum txheej txheem File"nyob rau hauv "Spike Explorer Settings" dialog yog tias koj tsis teem lub extraction parameter manually.

CMOS-MEA5000-System · 20231220 · www.multichannelsystems.com

CMOS-MEA-TOOLS
Spike Activity dhauview

Lub coordi

Cov ntaub ntawv / Cov ntaub ntawv

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Specifications

Cov lus qhia siv khoom

FAQs

TSEEM CEEB TSHAJ PLAWS

Taw qhia

HARDWARE

GENERAL SOFTWARE NTA

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Cov ntaub ntawv / Cov ntaub ntawv

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Multichannel systems IFB-C ​​Interface Board Multiboot System

Specifications

Cov lus qhia siv khoom

FAQs

TSEEM CEEB TSHAJ PLAWS

Taw qhia

HARDWARE

GENERAL SOFTWARE NTA

CMOS-MEA-CONTROL SOFTWARE

CMOS-MEA-TOOLS

Cov ntaub ntawv / Cov ntaub ntawv

Cov ntaub ntawv

Cia ib saib

Ncua tseg teb

Multichannel systems IFB-C Interface Board Multiboot System