Ƙirar tantanin halitta mai sarrafa kansa Quadcount
Ƙa'idar Tantanin halitta ta atomatik
Littafin koyarwa
Accuris Instruments
Sashen Kimiyya na Benchmark
Akwatin gidan waya 709, Edison, NJ 08818
Tel: 908-769-5555
E-mail: info@accuris-usa.com
Webshafin www.accuris-usa.com
Haƙƙin mallaka © 2020, Benchmark Scientific.
An kiyaye duk haƙƙoƙi.
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Kunshin abun ciki
QuadCount™ Kunshin lissafin salula ta atomatik ya haɗa da abubuwa masu zuwa.
Yawan Abun
Babban na'urar QuadCount™ 1
USB Memory Stick 1
Manual da sauri (PDF akan Memory Stick) 1
Littafin koyarwa (PDF akan Memory Stick) 1
Babban Cable Power 1
QuadCount™ Slides (Na zaɓi) 50 ea. kowane akwati
faifan maɓalli (Na zaɓi) 1
Na'urar daukar hoto na Barcode (Na zaɓi) 1
Thermal printer (Na zaɓi) 1
Lokacin karbar kunshin,
Duba cewa duk abubuwan da aka jera a sama suna cikin kunshin ku.
• Bincika na'urar a hankali don kowane lalacewa yayin jigilar kaya.
Tuntuɓi mai rarrabawa na gida ko info@accuris-usa.com idan wasu abubuwa sun ɓace ko sun lalace.
Duk wani hasara ko lalacewa dole ne ya kasance filed tare da mai ɗauka.
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Umarnin aminci
KARANTA DUK UMURNI KAFIN AMFANI
Tsanaki
Duba shigar da wutar lantarki voltage kuma a tabbata ya dace da mashin bango voltage.
• Bincika cewa an haɗa kebul ɗin wutar lantarki zuwa ƙasa mai tushe, mashin bango mai 3-pin.
• Bincika cewa kebul ɗin wutar yana ƙasa da kyau don guje wa yuwuwar girgiza wutar lantarki.
• Bincika cewa babban maɓallin wuta yana kashe lokacin da ke toshe kebul ɗin wuta zuwa mashin bango ko
lokacin cire wutar lantarki.
• Kunna amfani da babban maɓalli akan rukunin baya, jira kusan mintuna 2-3 don na'urar ta sake yi.
• Kada a saka wani abu na ƙarfe a cikin na'urar ta hanyar iska ta baya don guje wa girgiza wutar lantarki
haifar da rauni ko lalacewar na'urar.
• Sanya na'urar a wurin da akwai nisa na cm 10 daga wasu abubuwa don ba da damar dacewa
sanyaya iska.
• Kada a harhada na'urar. Idan ana buƙatar sabis, tuntuɓi Accuris Instruments ko mai izini
mai rarrabawa.
• Yi amfani da na'urorin haɗi masu izini kawai.
• Ma'aikaci ya kamata ya kasance da masaniyar fasahar dakin gwaje-gwaje da kirga tantanin halitta
hanyoyin da kuma amintaccen kula da samples.
• Yi aiki da na'urar a hankali kamar yadda aka bayyana a cikin wannan littafin.
Gargadi
• Baturi
Akwai baturin lithium a cikin na'urar. Maye gurbin shi da nau'in da ba daidai ba zai iya haifar da haɗari
fashewa. Bai kamata mai amfani ya maye gurbin wannan baturi ba; tuntuɓi Accuris sabis mai izini
tsakiya idan an buƙata.
• Sampda handling
Samples na iya ƙunsar abubuwa masu haɗari masu haɗari. Mai aiki ya kamata ya sa safar hannu yayin da
sarrafa duk samples.
• Sharar gida
Zubar da QuadCount™ Slides da aka yi amfani da su azaman sharar rayuwa kuma kar a sake amfani da su.
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Bayani dalla-dalla
QuadCountTM
Voltage AC 100 ~ 240 V, 50 ~ 60 Hz
Max na yanzu. 1.0 A, 50 W
Maƙasudin ruwan tabarau 4 x
Hasken haske 4W Green LED
Kamara
5 Mega pixels babban ƙuduri
monochrome CMOS image
firikwensin
Nauyi 5 Kg
Girman (W × L × H) 163 × 293 × 216 mm
Aunawa
kewayon maida hankali
1 x 104 ~ 1 x 107
sel/mL
Kwayoyin ganowa
diamita 5 ~ 60µm
Ma'auni gudun*
Yanayin sauri: ≈ 20s kowane gwaji
Yanayin al'ada: ≈ 30s kowane gwaji
Daidaitaccen yanayin: ≈ 100s kowane gwaji
Yankin kirgawa
Yanayin sauri: ≈ 0.15 µL
Yanayin al'ada: ≈ 0.9 µL
Daidaitaccen yanayin: ≈ 3.6 µL
QuadSlides™
(Cat. A'a. A'a.
E7500-S1
(Oda
daban)
Yawan nunin faifai 50 a kowane akwati (don gwaje-gwaje 200)
Sampda loading
girma 20 µL
Na'urorin haɗi
Kebul na wutar lantarki 1.5 m
Kebul na ƙwaƙwalwar ajiya yana goyan bayan USB 2.0
(Na zaɓi)
Maɓalli, Barcode
na'urar daukar hotan takardu, thermal
printer
Nau'in USB
*Lokacin ƙidayar salula na iya bambanta dangane da nau'in tantanin halitta da taro.
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Na'urar ta kareview
Gaba view
• Ƙofar mariƙin zamewa - Ana fitar da mariƙin zamewa daga / saka cikin na'urar.
• Nuni LCD - Preview, tafiyar matakai na kirgawa ta atomatik kuma ana nuna sakamakon.
• Maɓallan sarrafawa 3
Ƙofar mariƙin zamewa
Allon taɓawa LCD nuni
Maɓalli 3 (Allon saiti)
Maɓalli 1 (Saka/Fitar da mariƙin nunin faifai)
Maɓalli 2 (Allon Gida)
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Na baya view
• 3 tashoshin USB - faifan maɓalli, na'urar daukar hotan takardu, na'urar daukar hotan takardu ta thermal (na zaɓi), ko ƙwaƙwalwar USB sune
alaka da wadannan tashoshin jiragen ruwa.
• tashar tashar Ethernet – An haɗa kebul na LAN zuwa wannan tashar don keɓancewar PC.
• Canjin wuta - Babban ikon na'urar ON/KASHE iko.
• Wutar kebul na wuta - Ana haɗa kebul na wuta zuwa wannan soket.
USB Port
– Maɓalli
– Barcode Scanner
– Thermal Printer
- USB Memory
Ethernet Port
– PC Interface
Canjin Wuta
Wutar Kebul na Wuta
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Abubuwan da ke ciki
Bayanan Bayani na 3
Umarnin aminci 4
Bayanin samfur 5
Na'urar ta kareview 6
Gabatarwa
QuadCount™ – Mai ƙidayar salula ta atomatik 10
QuadSlides™ (50 nunin faifai don gwaje-gwaje 200 a kowane akwati, Cat. No. E5750-S1)
Farawa
Abubuwan da ake bukata 12
Asalin shigarwa 13
Ƙarfin wutar lantarki da nunin farko 14
Gabaɗaya aiki
Sample shiri 15
Basic aiki 16
Preview kafin kirga 18
Tsayawa yayin kirgawa
Saita kirga zaɓi 21
A. Canza rukunin masu amfani 22
B. Yanayin Saitin Ƙididdiga 23
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C. Ƙirƙirar Saiti 24
D. Gyara Saiti 27
E. Zabin Tashoshi
F. Shigar da tashar ID 30
Allon sakamako
A. Yin nazari ta hanyar Histogram 36
B. View hotuna sakamakon
C. Buga Sakamakon Ƙididdiga ta Wayoyin salula ta amfani da Thermal Printer 40
D. Ana fitar da Rahoton zuwa sandar ƙwaƙwalwar ajiyar USB
E. Ana fitar da bayanai (duk tarihi) zuwa sandar ƙwaƙwalwar ajiyar USB 43
F. Nuna sunayen tashar tashar
Saitin allo
A. Duba bayanan Firmware da Ana ɗaukaka Firmware 48
B. Ƙwaƙwalwar Ƙwaƙwalwar Ƙwaƙwalwa 50
C. Saita Kwanan Wata da Lokaci
Kulawa da tsaftacewa
Karin bayani
A. Matsalar harbi 54
B. Examples na kurakurai da sakamakon da ba daidai ba
C. Abubuwan da ke cikin bayanan sakamako da aka fitar a matsayin .csv file
D. Example da bayanin rahoton PDF 58
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43
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48
50
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54
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Gabatarwa
QuadCount™ – Ƙa'idar salula ta atomatik
QuadCount™ cikakken tsarin ƙidayar salula ce mai sarrafa kansa bisa madaidaicin fili mai haske
dabara don ƙidayar sel masu shayarwa. QuadCount™ yana amfani da tushen hasken LED mai ƙarfi,
Gano hoton CMOS (pikisal 5 Mega), madaidaicin XYZ stages da sarrafa hoto kan-slide
fasahohin don bincike mai sauri da daidaitattun ƙwayoyin cuta.
Ƙididdigar tantanin halitta ta amfani da QuadCount™ yana buƙatar manyan matakai 3, (1) tabon cell, (2) lodi
sample slide, da (3) kirgawa. An gauraya sel da launin shudi na trypan don bambanta tsakanin rayuwa da
matattun kwayoyin halitta. Tabo sampLe an pipetted a cikin faifan filastik da za a iya zubarwa (gwaji 4 a kowane nunin) da
Ana loda faifai a cikin Kayan Aikin QuadCount. Bayan loda faifan, tsarin gani
ta atomatik yana mai da hankali kan zamewar kuma kayan aikin ya samo da kuma nazarin hotuna
ta atomatik. Madaidaicin XYZ stages matsawa ta hanyoyin da aka saita don ɗaukar hotuna da yawa don
kowane tashar. Babban firikwensin CMOS mai mahimmanci yana samun hotuna masu haske na fili kuma yana aikawa
su zuwa tsarin da aka haɗa don sarrafa hoto da bincike. Duk tsarin kirgawa yana ɗauka
Minti 2 (a yanayin al'ada) kuma ana nuna sakamakon kirgawa akan allon taɓawa na LCD
a gaban kayan aiki.
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QuadSlides™ Slides (Slides 50 don gwaje-gwaje 200 a kowane akwati, Cat. No. E7500-S1)
QuadSlide™ hemocytometer na roba ne wanda za'a iya zubar dashi wanda ya hada da s 4ample tashoshi kwarkwasa
tare da Neubauer Ingantaccen tsari. Kowane tashoshi yana da rufaffiyar tsari na zurfin 100um da kuma a
hydrophilic surface. Madaidaicin iya aiki da farfajiya mai yaduwa yana tabbatar da cewa sel suna
a ko'ina cikin rarraba kuma wannan yana tabbatar da ingantaccen bincike. Ana iya amfani da QuadSlides™ don mammalian
kirga tantanin halitta tare da Kayan aikin QuadCount, amma kuma ana iya amfani da su don hanyoyin kirgawa na hannu.
Ma'aunin ma'auni na tattarawar tantanin halitta 1 x 104 ~ 1 x 107 kowace ml lokacin amfani da QuadCount
Kayan aiki.
Ƙididdiga ta salula: shirya ƙwaƙƙwaran sel don kirgawa da haɗa dakatarwar tantanin halitta da trypan
blue a rabo ɗaya zuwa ɗaya. Kowane tashoshi na QuadSlide™ yana cike da 20 μL na cakuda sannan kuma
loda cikin kayan aikin QuadCount™. Bayan an gama bincike, za a nuna sakamakon.
Rike akwatunan QuadSlide™ a tsaye kuma a zafin daki. Ya kamata kowane zane-zane ya kasance
da aka yi amfani da shi nan da nan bayan buɗe fakitin mutum wanda aka hatimce. Bi ainihin hanya daki-daki
a cikin sashin Umarnin don amfani.
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Farawa
Pre-bukatun
Don aiki na yau da kullun da kwanciyar hankali na na'urar, yanayin muhalli ya kamata ya kasance
hadu.
• Zafin ɗaki tsakanin 20 ~ 35 °C (68 zuwa 95 °F)
Ba'a ba da shawarar yin aiki da na'urar a yanayin ƙarancin zafin jiki (kasa da 10 ° C)
A cikin yanayin sanyi, dumama na'urar don aƙalla mintuna 10 kafin amfani.
• Dangantakar zafi tsakanin 0 ~ 95 %.
• Sanyawa a wurin da babu gurɓataccen iskar gas ko wasu abubuwa masu lalata.
• Shigarwa a wuri mara ƙura ko wasu barbashi na iska.
• Guji hasken rana kai tsaye, girgizawa, da kusanci ga filayen maganadisu ko na lantarki.
Kar a sanya kowane abu mai nauyi a saman na'urar.
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Asalin shigarwa
1. Cire akwatin QuadCount™ kuma sanya
na'urar a kan lebur, matakin da bushe.
2. Toshe kebul na wuta mai rakiyar cikin
wutar lantarki soket.
3. Haɗa kowane yanki na zaɓi (keypad,
Barcode Scanner, ko thermal printer) zuwa ga
Tashar USB idan ana so.
4. Toshe kebul na wutar lantarki cikin abin da ya dace
rated bango kanti kuma danna wutar lantarki
ku ON.
Duba cewa babban wutar lantarki yana cikin I (ON)
matsayi.
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Ƙarfafawa da nunin farko
1. Da zarar babban iko ya kunna, da
Ana nuna hoton taya akan taɓawar LCD
allo. Lokacin da aka gama booting, da
farawa tsari yana farawa da ciki
mota stages fara motsi.
2. Ana nuna fara ci gaba yayin da
sarrafawa.
3. Lokacin farawa ya ƙare, mai riƙe da nunin faifai
An fitar da shi, kuma ana nuna allon gida akan
LCD tabawa.
4. Bayan loda nunin faifai tare da sample, na'urar
yana shirye don ƙidaya.
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Gabaɗaya Aiki
Sample Shiri
Abubuwan da ake buƙata: dakatarwar salula, 0.4% trypan blue, micro tube 1.5ml, pipette, tukwici, da
QuadSlides™. Ya kamata a yi shiri a wuri mai tsabta don guje wa gurɓatar ƙura (ƙura a kan
nunin faifai ko a cikin samples zai rage girman kirga daidaito).
MATAKI 1. Shirya abubuwan da ake bukata.
Mataki na 2. Sanya 20 μL na blue trypan a cikin ƙaramin bututu kuma ƙara daidai girman tantanin halitta.
dakatarwa.
NOTE: Kafin sampkunna dakatarwar tantanin halitta, a hankali mayar da sel aƙalla sau 6
(ku kula don guje wa kumfa kuma ku duba idan akwai tantanin halitta clumps ko agglomerates)
A sampling yakamata ya kasance a tsakiyar dakatarwar tantanin halitta, ba a saman ko ƙasa ba.
Mataki na 3. Mix da sampLe a cikin ƙananan bututu ta hanyar pipetting vial sau 3 ~ 5 a hankali.
NOTE: Yi hankali kada ku haifar da kumfa.
Mataki na 4. Load 20 μL na tantanin halitta sampshiga kowane tashar QuadSlide™.
NOTE: sampLes ya kamata ya kasance daga tsakiyar dakatarwar tantanin halitta, ba daga saman ko kuma ba
kasa, da kuma tabbatar da cewa babu kumfa da ke shiga tashar tashoshi.
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Basic Aiki
MATAKI 1. Saka QuadSlide™ wanda aka loda tare da samples zuwa cikin mariƙin nunin faifai.
NOTE: Tabbatar da kibiya akan nunin nuni zuwa kayan aiki.
MATAKI 2. Latsa maɓallin farawa don fara kirga hanyoyin. Mai riƙe da nunin zaya ja da baya
ta atomatik, kuma ana yin sa ido ta atomatik kafin kirga kowane sample.
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Mataki na 3. Ana nuna ci gaban ƙidayar kamar yadda aka nuna a hoto mai zuwa. Domin kammala kowanne
sample, sakamakon ƙidaya (naúrar: x104
/ml) suna nunawa.
Mataki na 4. Da zarar an gama kirgawa, za a fitar da mariƙin nunin faifai ta atomatik. Cire QuadSlide™
daga mariƙin nunin faifai.
1
1
Gida
260 40 15
340 140 41
500 420 84
200 100 50
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Preview kafin kirgawa
A kan allon da za ku iya ganin sel, matsa allon sau biyu don sa gumakan su ɓace.
Don sake dawo da gumaka, matsa allon sau biyu.
Mataki na 1. Load da nunin faifai kuma latsa Review maballin.
Mataki na 2. Zaɓi tashoshi don farawaview.
MATAKI 3. Matsayi da Mayar da hankali yana faruwa ta atomatik
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Mataki na 4. Duba hoton tantanin halitta na tashar da aka zaɓa.
MATAKI 5. Danna Alama, kuma alamar ganowa tana nunawa. Za a iya canza ma'anar rayuwa/Matattu
a nan stage.
MATAKI 6. Kidaya
1
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Tsayawa Lokacin Kidaya
MATAKI 1. Don tsayar da kayan aiki yayin kirgawa, Danna maɓallin TSAYA.
MATAKI 2. Ana nuna akwatin saƙon tabbatarwa kamar yadda aka nuna hoton da ke gaba.
Danna maɓallin Ci gaba don tabbatar da tsayawa.
Mataki na 3. Da zarar an tabbatar da ƙidayar tasha, ana dakatar da duk matakan da suka rage, da mariƙin nunin faifai
ana fitarwa ta atomatik.
1
1
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Saita zaɓuɓɓukan kirgawa
Ana iya aiwatar da ayyuka masu zuwa daga Fuskar allo.
Saitin zaɓuɓɓuka don kirgawa
Mai amfani: 1/2/3
Ana iya sarrafa bayanan da aka adana ta atomatik da saitattun saiti ga kowane mai amfani.
Yanayin ƙidaya: Mai sauri/Na al'ada/Madaidaici
Jimlar wurin kirgawa (yawan hotuna) ya bambanta ga kowane yanayin ƙidayar.
Yanayi mai sauri: ≈ 0.15 µL (Firam 1)
Yanayin al'ada: ≈ 0.9 µL (Frames 6)
Yanayi daidai: ≈ 3.6 µL (Framumai 24)
Saita
Matsaloli masu iya canza mai amfani don gano tantanin halitta
Akwai ƙayyadaddun ƙayyadaddun saiti guda uku
Tashoshi
Yanke shawarar tashoshin da za a auna
Akwatin fari: tashar da aka kunna
Akwatin launin toka: tashar da aka kashe
Danna tashar don kunna tsakanin kunnawa da kashewa.
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A. Canza Ƙungiya Mai Amfani
QuadCount™ yana ba da keɓaɓɓen tarihin sakamako ga ƙungiyoyin masu amfani (1,2 da 3).
Ƙungiyar mai amfani tana da amfani don sarrafa saitattun masu amfani da sakamako masu yawa da aka adana ta atomatik bayan ƙirgawa. The
sakamakon da aka ajiye ta atomatik (review screen) ana samun dama ga ƙungiyar masu amfani da ke aiki a lokacin
an kama sakamakon.
Lura: Review kuma Jerin saitattun masu amfani ya dogara da ƙungiyar mai amfani. Saboda haka, kafin zabar saitaccen mai amfani ko
danna review, duba rukunin masu amfani.
Mataki 1. Danna maɓallin Mai amfani.
Mataki 2. Zaɓi Mai amfani 1/2/3.
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B. Saita yanayin ƙidaya
QuadCount™ yana ba da yanayin kirga guda uku (Sauri / Al'ada/Madaidaicin yanayin) bisa ga
wurin kirgawa. An ƙera QuadCount™ don ɗaukar firam ɗin da yawa a kowane tashoshi ta amfani da XYZ
stage. Kowane firam ɗin hoto ɗaya yana rufe ƙarar 0.15 µL. Da ƙarin hotuna da aka dauka, mafi girma da
daidaiton sakamako.
Zaɓi yanayin ƙidaya dangane da buƙatu, koma zuwa tebur mai zuwa.
Yanayin ƙidaya
adadin
Frames kama
ta tashar
An yi nazari
girma
Kidaya
lokaci
per
jam'iyya
Aikin nema
Yanayi mai sauri 1 0.15µL ≤ 20s
Lokacin da kake son samun sakamako da sauri kuma
yi m kimanta lambobin cell.
Yanayin al'ada
(tsoho) 6 0.9µL ≤ 30s
Lokacin da kake son samun sakamako da
m daidaito da sauri (kamar
tsarin subculture na gaba ɗaya)
Daidaitaccen yanayin 24 3.6µL ≤ 100s
Lokacin da kuke buƙatar takamaiman sakamako ko ƙidaya
Kwayoyin daga ƙananan taro sample.
NOTE: idan maida hankali tantanin halitta bai wuce 5X104 ba
sel/ml, Ana bada shawarar madaidaicin yanayin.
MATAKI 1. Danna maɓallin Ƙidaya Yanayin.
2
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MATAKI 2. Zaɓi yanayin ƙidaya.
NOTE: Ana amfani da saitin akan duk tashoshi masu kunnawa.
C. Ƙirƙirar Saiti
Masu amfani za su iya sarrafa abubuwan da aka saita mai amfani. (Ana samun saitattun masu amfani 5 kowane rukunin mai amfani)
Ba za a iya cirewa ko gyara madaidaitan saiti guda 3 ba.
MATAKI 1. Don ƙirƙirar saiti na kanku, danna maɓallin Saiti.
MATAKI 2. Danna maɓallin Plus.
PT
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MATAKI 3. Zaɓi ɗaya daga cikin tsayayyen saiti guda 3 (Universal, Small, Angular),
kuma danna akwatin rubutu mara kyau kusa da Index.
MATAKI 4. Buga sunayen Index da Saiti ID.
Babban TPT na farko T
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Mataki na 5. Daidaita sigogi 3 bisa ga buƙatun.
(Gating size, Aggregation Level, Live/Dead definition).
Mataki na 6. Shirye don ƙidaya tare da saiti na musamman.
PT
27
D. Gyara Saiti
MATAKI 1. Don gyara saitattun naku, danna maɓallin Saiti.
Mataki 2. Zaɓi maɓallin saiti wanda kuka ƙirƙira.
MATAKI 3. Daidaita sigogin saiti na ku.
PT
PT
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MATAKI 4. Danna maɓallin Ajiye don kiyaye sigogin da aka canza.
MATAKI 5. Don share saitattun naku, danna maɓallin Share.
Babban darajar PT
PT
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E. Zabin Tashoshi
Tashoshi hudu a cikin QuadSlide™ ana iya kunna ko kashe su daban-daban.
MATAKI 1. Danna lambobin tashar don a kashe/kunna. (An kashe: Akwatin launin toka, An kunna: Akwatin farin)
MATAKI 2. Danna maɓallin farawa don ƙidaya nan da nan.
2
2
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F. Shigar da tashar ID
Za'a iya cika suna/Gano tasha tare da zaɓin ID na Channel. Zaɓi "Channel
ID" kamar yadda aka nuna a ƙasa kuma shigar da sunan tashar da ake so. (Sunan na iya zama takamaiman
irin cell.)
ID ɗin zai iya ƙunsar iyakar haruffa 20 na haruffa da wasu haruffa na musamman.
MATAKI 1. Danna maɓallin ID na Channel.
MATAKI 2. Zaɓi tashar da ake so (1 zuwa 4).
MATAKI 3. Buga sunayen da ake so ga kowane Tashoshi.
Tashar ID
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MATAKI 4. Danna Maballin Baya.
Mataki na 5. Shirye don ƙidaya.
JurkatT
JurkatT
NIH
Hela
U937
Tashar ID
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Don cike duk ID na tashar don nau'in tantanin halitta iri ɗaya
MATAKI 1. Danna All button.
MATAKI 2. Shigar da sunan da ake so (ko nau'in tantanin halitta kuma danna maɓallin Ok.
MATAKI 3. Tabbatar da cewa ID ɗin ya cika ta atomatik don duk tashoshi 4, sannan danna maɓallin baya.
Tashar ID
JurkatT All
JurkatT_1
JurkatT_2
JurkatT_3
JurkatT_4
Tashar ID
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Yin amfani da na'urorin shigar da kayan haɗi: na'urar daukar hotan takardu, faifan maɓalli na USB ko madannin USB
(na zaɓi)
faifan maɓalli da na'urar daukar hotan takardu ba zaɓi bane. Tuntuɓi mai rarrabawa na gida idan an buƙata.
Haɗa na'urar shigarwa zuwa tashar USB a gefen baya na na'urar. Lokacin da ya dace
an haɗa kuma an gane shi, gunki yana bayyana akan ma'aunin matsayi.
Shigarwa
Na'ura
Amfani
faifan maɓalli
1. Shigar da ID na tashar kuma danna maɓallin "Shigar".
2. Siginan kwamfuta yana motsawa zuwa akwatin ID na tashar ta gaba
(Za a iya amfani da maɓallin jagora don matsar da siginan kwamfuta.)
Barcode
Scanner
1. Bincika lambar lamba mai ɗauke da sunan tashar tashar.
2. Akwatin ID na Channel yana cike da sunan ID daidai, kuma siginan kwamfuta yana motsawa zuwa
akwatin na gaba idan an shigar da shi cikin nasara.
MATAKI 1. Haɗa faifan maɓalli ko na'urar daukar hotan takardu ta hanyar tashar USB a gefen baya na QuadCount.
Bincika idan alamar tana nan a saman. Danna maɓallin na'urar daukar hotan takardu a sama da tashar
Akwatunan ID.
Tashar ID
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MATAKI 2. Taɓa babban akwatin rubutu mara komai kuma shigar da ID na tashoshi 4 ta amfani da faifan maɓalli da aka haɗa ko
na'urar daukar hotan takardu (duba teburin da ke sama). Matsakaicin adadin ID na tashar tashar shine haruffa 20
haruffa ko wasu haruffa na musamman.
Mataki na 3. Tabbatar da cewa an cika akwatunan ID na tashar har zuwa 4 daidai, sannan danna maɓallin Baya.
Tashar ID
JurkatT
NIH
Hela
U937
Tashar ID
35
Allon sakamako
Ana yin ayyuka masu zuwa akan allon sakamako bayan ƙidaya.
Bayan kammala kirga tantanin halitta, ana bayar da histograms na girman rabon tantanin halitta da sakamakon sakamakon.
Yayin viewA cikin histogram, yana yiwuwa a canza sigogin girman girman tantanin halitta. QuadCount™ na iya
samar da duka histograms don kowane tashoshi da kuma haɗaɗɗen tarihin duk tashoshi.
QuadCount™ na iya gano abubuwa diamita 5 ~ 60µm. Koyaya, an saita tsarin gating
ta tsohuwa don ƙidaya daga 8µm saboda yawancin layin salula na yau da kullun suna da girman farawa a ko
fiye 8µm.
NOTE: Idan kuna son ƙidaya sel ƙasa da 8 µm, canza ma'aunin gating girman tantanin halitta a cikin
histogram.
Juya tsakanin histogram da hoton sakamako bayan zaɓin tasha.
12-34
36
Latsa ko maɓalli don ganin sakamakon hotunan tashoshi da aka zaɓa.
Komawa zuwa tsoho : Saitunan da aka canza suna komawa zuwa saitunan tsoho.
Ƙirƙirar saiti : Za a iya adana saitunan da aka gyara azaman sabon saiti.
▪ Ajiye a cikin saiti na yanzu : Ana iya adana saitunan da aka canza a saiti na yanzu (Wannan shine
ba samuwa a cikin tsayayyen saiti).
▪ Aiwatar da duka: Ana amfani da saitunan da aka canza zuwa duk tashoshi.
A. Yin nazari ta hanyar Histogram
MATAKI 1. Danna lambar tashar don dubawa, kuma canza zuwa gunkin tarihin.
MATAKI 2. Danna Duk zuwa view matsakaicin bayanan duk tashoshi.
12-34
37
Mataki na 3. Matsar da ginshiƙan biyu kuma daidaita girman gating ɗin tantanin halitta.
Mataki na 4. Bincika teburin sakamako na jimillar sel, yawan rayuwa, da yuwuwar %.
12-26
38
B. View Hotunan Sakamako
QuadCount™ yana ba da hotunan sakamakon bayan kirgawa. Ana samun hotuna ɗaya ko fiye da
an bincika kowane tashoshi, kuma adadin hotuna ya dogara da yanayin ƙidayar da aka zaɓa. Sakamakon "
hoto” allon yana nuna hotunan da aka bincika tare da rayayyun sel waɗanda aka zagaye cikin kore da matattun ƙwayoyin da aka yi dawafi da ja.
MATAKI 1. Danna tashar da kake son dubawa, kuma canza zuwa gunkin Hoto.
MATAKI 2. Daidaita ma'anar tantanin halitta Live/Matattu.
39
MATAKI 3. Danna Data icon.
MATAKI 4. Review adadin Rayayyun Kwayoyin Rayu da Dorewa %.
60
40
C. Buga Sakamakon Ƙididdiga ta Wayoyin salula ta amfani da firinta na Thermal
QuadCount™ na iya amfani da firinta mai zafi don buga sakamakon kirgawa.
Thermal printer na zaɓi ne. Tuntuɓi Accuris Instruments ko mai rabawa na gida don
oda bayanai.
Mataki 1. Haɗa firinta na thermal zuwa tashar USB a gefen baya na na'urar.
Tabbatar cewa gunkin yana nan akan ma'aunin matsayi, yana nuna cewa an gane shi.
Danna Maɓallin Buga.
Example
41
D. Ana fitar da rahoto zuwa sandar ƙwaƙwalwar ajiyar USB
Ana iya fitar da rahoton sakamakon kirgawa azaman PDF zuwa sandar ƙwaƙwalwar ajiyar USB. Rahoton PDF
yana nuna cikakken bayani, hoton tantanin halitta da kuma tarihin rarraba girman tantanin halitta.
Da fatan za a yi amfani da sandar žwažwalwar ajiya na USB wanda aka haɗa tare da QuadCount™ ko wani
An tsara shi zuwa FAT32 ko NTFS file tsarin. Sandunan žwažwalwar ajiya na USB wanda aka tsara zuwa tsohuwar-FAT file
tsarin ba a tallafawa.
Idan tsohon-FAT File Ana haɗa sandar ƙwaƙwalwar ajiyar tsarin, gunkin ƙwaƙwalwar USB zai nuna, amma
saƙon kuskure "Ƙwaƙwalwar USB mara tallafi" zai bayyana lokacin ƙoƙarin fitar da bayanai ko a
rahoto.
Mataki 1. Haɗa sandan ƙwaƙwalwar USB zuwa tashar USB a gefen baya na QuadCount.
Tabbatar cewa gunkin yana nan akan ma'aunin matsayi, yana nuna cewa an gane shi.
Danna maɓallin Export PDF.
Mataki 2. Akwatin maganganu na ci gaba ya bayyana yana nuna cewa ana ci gaba da fitar da rahoton.
42
Mataki na 3. Da zarar akwatin maganganu na ci gaba ya ɓace kuma saƙon sanarwa ("Nasara Export") shine
wanda aka nuna akan ma'aunin matsayi, zaku iya cire sandar ƙwaƙwalwar USB daga tashar USB.
NOTE: Idan an cire sandan ƙwaƙwalwar USB kafin saƙon “fitarwa” ya ɓace, sakamakon
file ana iya lalacewa.
43
E. Ana fitar da bayanai (duk tarihi) zuwa sandar ƙwaƙwalwar ajiyar USB
Sakamakon, da aka yi rikodin a cikin ƙungiyar mai amfani na yanzu (Duk tarihin), ana iya fitar dashi zuwa sandar ƙwaƙwalwar ajiyar USB.
Ana adana bayanan sakamako ta atomatik a cikin ƙwaƙwalwar na'urar ƙungiyar masu amfani da aka kunna. Ta hanyar amfani da
Siffar “Exporting Data”, ana fitar da bayanan azaman CSV file (tsarin waƙafi-rabu-daraja) wanda
za a iya bude ta Microsoft Excel.
Da fatan za a yi amfani da sandar žwažwalwar ajiya na USB wanda aka haɗa tare da QuadCount™ ko wani
An tsara shi zuwa FAT32 ko NTFS file tsarin. Sandunan žwažwalwar ajiya na USB wanda aka tsara zuwa tsohuwar-FAT file
tsarin ba a tallafawa.
Idan tsohon-FAT File Ana haɗa sandar ƙwaƙwalwar ajiyar tsarin, gunkin ƙwaƙwalwar USB zai nuna, amma
saƙon kuskure "Ƙwaƙwalwar USB mara tallafi" zai bayyana lokacin ƙoƙarin fitar da bayanai ko a
rahoto.
QuadCount™ yana adana bayanai ta atomatik har zuwa rikodin 1000 a kowace ƙungiya.
Mataki 1. Zaɓi ƙungiyar mai amfani.
Mataki 2. Danna Review.
3
44
Mataki 3. Ana nuna sakamakon da aka ajiye ta atomatik don ƙungiyar mai amfani da aka zaɓa.
Haɗa sandar ƙwaƙwalwar USB zuwa tashar USB a gefen baya na kayan aiki.
Tabbatar cewa gunkin yana nan akan ma'aunin matsayi, yana nuna an gane shi.
Danna Maɓallin fitarwa CSV.
Mataki na 4. Akwatin maganganu na ci gaba ya bayyana yana nuna cewa ana ci gaba da fitar da bayanai.
Bayanan CSV yanzu suna fitarwa…
45
Mataki 5. Da zarar akwatin maganganu na ci gaba ya ɓace kuma saƙon sanarwa "An fitar da duk bayanan" shine
wanda aka nuna akan ma'aunin matsayi, cire ƙwaƙwalwar USB daga tashar USB.
NOTE: Idan an cire sandar žwažwalwar ajiya na USB kafin sakon "bayanai yana fitarwa" ya ɓace
sakamako file ana iya lalacewa.
46
F. Nuna sunayen tashar tashar
Mataki 1. Don ganin kowane sunan Channel ID, danna Channel ID.
Don komawa zuwa lambobin tashar, danna Baya.
47
Saitin allo
FN
48
A. Duba bayanan Firmware da Sabunta Firmware
Mataki 1. Danna F/W info & Update, da kuma haɗa na USB memory stick wanda ya ƙunshi dace
sabunta firmware files.
Mataki 2. Zaɓi nau'in firmware don ɗaukakawa (Main ko Nuni).
Idan USB ba a haɗa sandar žwažwalwar ajiya ba ko bai ƙunshi shirin ɗaukaka ba files, sako
za a nuna.
Mataki 3. Danna Update button.
Mataki 4. Ana ɗaukaka
Shafin na yanzu: 1.0
Sabon sigar: 1.01
49
Mataki 5. Na'urar za ta sake farawa ta atomatik tare da sabunta firmware version.
Tabbatar da cewa sigar(s) sun sabunta yadda ya kamata.
Mataki na 6. Bayan kamar minti 1 kuma farawa ya cika, canza wutar zuwa kashe sannan kuma baya
a kan sake don barga aiki.
NOTE: Lokacin da saƙo mai zuwa "Don Allah jira..." ya bayyana akan allon ƙaddamarwa bayan
sabunta firmware, da fatan za a jira 2 ~ 3 mintuna. Kar a kashe na'urar nan da nan.
50
B. Ƙwararren Ƙwaƙwalwar Ƙwaƙwalwa (Duba umarnin da aka haɗa tare da kayan aikin Bead QC don ƙarin
bayani.)
Mataki 1. Danna maɓallin Bead QC.
Mataki 2. Loda madaidaicin nunin faifai tare da gaurayawan ƙugiya masu dacewa da aka ƙara zuwa sampda chambers
kuma danna maɓallin START.
51
Mataki na 3. Kidayar
Mataki 4. Duba sakamakon data.
Mataki 5. Duba Hoton Histogram da Bead.
200 12 Duba hoton
TSAYA
12-34
Gida
200 12 Duba hoton
320 15 Duba hoton
400 17 Duba hoton
350 19 Duba hoton
52
Mataki 6. Komawa zuwa Fuskar allo.
C. Saita Kwanan Wata da Lokaci
MATAKI 1. Danna Maɓallin Lokaci.
Mataki na 2. Daidaita kwanan wata da lokaci daidai.
53
MATAKI 3. Danna maɓallin Saita don adana ƙimar da aka daidaita.
MATAKI 4. Komawa kan Fuskar allo.
22
54
Kulawa da Tsaftacewa
Kayan aikin QuadCount™ baya buƙatar kulawa na yau da kullun ko musanyawa akai-akai
sassa ko sassa. Tsaftace saman na'urar ta waje ta amfani da zane mai laushi. isopropyl
Ana iya amfani da barasa ko ruwan da aka lalata tare don tsaftace gidaje.
Kada ka ƙyale tsabtace ruwa ko mafita su shiga cikin gidaje.
55
Shafi A. Matsalar harbi
Maganin Sanadin Matsala
Na'ura
Ba a kunna ba
Maɓallin wuta yana cikin wurin kashewa. Duba maɓallin wuta a bayan naúrar.
Babu wuta daga kanti. Duba tushen wutar lantarki.
Rashin wutar lantarki mara kyau. Sauya kebul.
Sakamako mara inganci
Maganin tabo ya ƙare ko
an gurbata. Yi amfani da sabon maganin tabo ko tace maganin.
Matattun sel masu yawa da yawa.
Gwada sake, pipette cakuda tantanin halitta a hankali don haɗawa
sel kafin ƙara zuwa ɗakunan faifai.
(duba hoton tantanin halitta don wuce gona da iri clumping cell ko
agglomerates)
Sampling kuskure
✓ Maimaita matakan bututun tantanin halitta yadda ya kamata
cakuda don tsari mai lalata.
✓ Kafin sampling da cell dakatar, a hankali
mayar da sel aƙalla sau 6 ta a hankali
bututu sama da ƙasa
✓ Sample daga tsakiyar dakatarwar tantanin halitta
tube, ba kusa da saman ko kasa ba.
Kumfa a cikin ɗakunan nunin faifai Kula da hankali don guje wa kumfa lokacin
pipetting da lodi samples cikin slide
ƙananan ƙwayar salula
(≤5 x 104
)
A sake gwadawa ta amfani da Madaidaicin yanayin.
Girman tantanin halitta ya yi ƙasa da 10µm
ko kusa da 10µm.
Canja ma'aunin girman gating a cikin histogram.
Rabon trypan blue a cikin
sample yayi tsayi da yawa ko kadan..
Haxa dakatarwar tantanin halitta da blue trypan a 1:1
rabon girma.
Too Haske ko duhu hoton
Mix cell suspension da trypan blue 1:1.
Idan ba a warware matsalar ba, tuntuɓi Accuris ko
mai rabawa na gida.
Ana ganin tsarin grid ko layi
a cikin hotunan sakamakon.
A sake gwadawa ta amfani da wani nunin faifai.
Idan matsalar tana faruwa akai-akai, tuntuɓi yankin ku
mai rarrabawa.
Bayanan da aka fitar ko
Rahoton shine
gurbatattu
An cire ƙwaƙwalwar USB
kafin nunawa
saƙon sanarwa
Jira har sai bayan saƙon sanarwar ya bayyana,
sannan cire memorin USB.
USB memori
Ba a Haɗa zuwa
na'urar
An tsara ƙwaƙwalwar USB
zuwa ex-FAT ko NTFS file tsarin.
Yi amfani da ƙwaƙwalwar USB wanda aka haɗa tare da QuadCount
kunshin ko wani wanda aka tsara zuwa FAT32 file tsarin
56
Idan trypan blue ko kafofin watsa labarai ya gurɓata ko ya ƙunshi kowane tarkace wanda yayi kama da girmansa da siffarsa
zuwa sel, wannan zai haifar da sakamako mara kyau.
Karin bayani B.
Examples na kurakurai da sakamakon da ba daidai ba
1. Kuskuren "Maƙarƙashiya".
2. Kuskuren "Mai Girma".
57
3. "Sampda kuskure"
Kwayoyin sun haɗu sosai A sampleda aka ɗora a cikin faifan ya bushe
4. Maganin gurɓataccen tabo
Sel gauraye da gurɓataccen ruwan shuɗi (Comparative Hoton) Sel da aka gauraye da taceccen blue trypan
58
Shafi C. Abubuwan da ke cikin Bayanan Sakamakon
fitar dashi azaman .csv file:
Teburin tarihi (Excel data) ya ƙunshi abubuwa masu zuwa.
Ƙungiyar mai amfani da aka zaɓa
File halitta Kwanan wata da lokacin da file an halicce shi
Lambar tashar tashar tashar
Sunan tashar ID Channel
Kwanan Aunawa Kwanan Wata
Lokacin Aunawa Lokaci
Jimlar tantanin halitta
[x10^4/ml] Jimlar sakamakon ƙidaya tantanin halitta
(x 1X104 Kwayoyin/ml)
(Sakamakon ƙidayar da aka canza)
Tantanin halitta mai rai
[x10^4/mL] Sakamakon Kidaya tantanin halitta
(x 1X104 Kwayoyin/ml)
(Sakamakon ƙidayar da aka canza)
Matattu cell
[x10^4/mL] Matattu sakamakon ƙidaya tantanin halitta
(x 1X104 Kwayoyin/ml)
(Sakamakon ƙidayar da aka canza)
Yiwuwar Kwayoyin Halitta (%)
59
Karin bayani D.
Example da bayanin rahoton PDF
60
Duk abubuwan da ke cikin wannan littafin suna kiyaye su ta Amurka da dokokin haƙƙin mallaka na duniya kuma ba za su iya kasancewa ba
sake bugawa, fassara, bugawa ko rarrabawa ba tare da izinin mai haƙƙin mallaka ba.
QuadCountTM Manual Umarnin
WebYanar Gizo: http://www.accuris-usa.com
E-mail: info@accuris-usa.com
Accuris Instruments (ɓangare na Kimiyyar Kimiyya na Benchmark)
Farashin 709
Edison, NJ 08818.
PH: ku
Fax: 732.313.7007
Bayanin da ke cikin wannan littafin an siffanta shi da dai dai yadda zai yiwu kuma ya dace da na baya-bayan nan
sigar firmware, amma ana iya canza shi ba tare da izini ko sanarwa ba.
Haƙƙin mallaka ©2020, Accuris Instruments.
An kiyaye duk haƙƙoƙi
Takardu / Albarkatu
 |
ACCURIS Quadcount Mai sarrafa tantanin halitta [pdf] Jagoran Jagora Ƙididdiga Ƙididdiga Mai sarrafa kansa, Ƙwararrun salula mai sarrafa kansa, counter cell, counter |
