GALENVS Plasmid Miniprep Kit User Guide
GALENVS Plasmid Miniprep Kit

Features

Features

Installation instruction

  1. Remove the protective foil.
    Installation instruction
  2. Resuspend bacterial pellet in 200µl of Resuspension Buffer then add to Lysis Buffer (columns 1 and 7).
    Installation instruction
  3. Place plate into the miQron, taking care that the label is facing outward.
    Installation instruction
  4. Insert two combs.
    Installation instruction
  5. Select the PM Kit protocol and press  Icon
    Installation instruction
  6. At Stop 1, add 100µl of Neutralization Buffer to columns 1 and 7.
    Installation instruction
    Place plate back in miQron and continue the program.
  7. At Stop 2, add 400µl of Binding Buffer to columns 1 and 7.
    Installation instruction
    Place plate back in miQron and continue the program.

When program is complete, discard combs then remove plate from miQron.
Columns 5 and 11 contain the purified DNA elution.
Installation instruction

PMKit miQron protocol parameters

Step Name Column Volume (p1) Time (sec) Mixing Speed (1-10) Dry Time (sec) Magnet Capture Time (sec)
Lysis 1 & 7 400 480 3 0 n/a
Stop 1- Neutralization 1& 7 500 120 5 0 75
Discard MNP 2 & 8 600 12 10 0 n/a
Stop 2- Binding 1& 7 900 60 5 0 50
Wash #1 3 & 9 600 60 5 0 50
Wash #2 4 & 10 600 60 5 60 50
Elution 5 & 11 50 60 5 0 50
Discard MNP 2& 8 600 6 10 0 0

Documents / Resources

GALENVS Plasmid Miniprep Kit [pdf] User Guide
Plasmid Miniprep Kit, Plasmid, Miniprep Kit, PM0016, PM0016-12

References

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