HK-GFP-1 GFP Capture Kit
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Specifications:
- Product Name: heliX + GFP CAPTURE KIT with red dye Ra
- Manufacturer: Dynamic Biosensors GmbH
- Model: HK-GFP-1 v3.1
- Key Features:
- 2 spots with 2 different anchor sequences for DNA-encoded
addressing - Spot 1 functionalized with the capture molecule
- Spot 2 used as real-time reference
- 2 spots with 2 different anchor sequences for DNA-encoded
Product Description:
Order Number: HK-GFP-1
Contents and Storage Information:
| Material | Concentration |
|---|---|
| GFP binding protein – Ligand strand Green | 500 nM |
| Adapter strand 1 – Ra Black | 400 nM |
| Adapter strand 2 – Ra – lfs White | 200/250 nM |
Product Usage Instructions:
Measurement Process:
- The GFP-tagged ligand is captured on the surface during the
measurement run. - Measure the analyte binding kinetics or conformational change
upon analyte binding. - Regenerate the surface by injecting a high pH solution to
return it to the original state.
Preparation:
Assay Setup in heliOS:
1. Go to heliOS > create a New Assay Workflow > add Custom
Assay > load Kinetics with Capture > modify parameters based
on your needs and run the assay.
Suggested assay parameters:
Flow rate, time, LED power, etc., are within the heliOS assay.
For further details, contact support@dynamic-biosensors.com.
FAQ:
Useful Order Numbers:
- Chip with 2 detection spots: Order No ADP-48-2-0
- Passivation of chip surface: Order No SOL-PAS-1-5
- Regeneration of chip surface: Order No SOL-REG-1-5
Contact Information:
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich Germany
Bruker Scientific LLC
40 Manning Road, Mamming Park Billerica, MA 01821 USA
Order Information:
order@dynamic-biosensors.com
Technical Support:
support@dynamic-biosensors.com
Note: For detailed technical specifications and
additional information, refer to the official product manual at
www.dynamic-biosensors.com.
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heliX +
User Manual
GFP CAPTURE KIT
with red dye Ra
Dynamic Biosensors GmbH HK-GFP-1 v3.1
Key Features
· This kit is designed for capture of GFP-tagged ligands using GFP binding protein. · Compatible with heliX® Adapter Chip. · Includes Adapter strands and Ligand strand modified with GFP binding protein for 20 regenerations. · For functionalization of Spot 1 and Spot 2. · Adapter strands 1 and 2 carry a moderately hydrophilic red dye (Ra) with a single positive net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing. Spot 1 is functionalized with the capture molecule while Spot 2 is used as real-time reference.
Product Description
Order Number: HK-GFP-1
Table 1. Contents and Storage Information
Material
Cap
GFP binding protein [1] – Ligand strand Green
Adapter strand 1 – Ra
Black
Adapter strand 2 – Ra – lfs
White
Concentration 500 nM 400 nM 200/250 nM
Amount 2 x 100 µL 2 x 100 µL 2 x 200 µL
For research use only.
This product has a limited shelf life, please see expiry date on label. After preparation of ready to use solution the expiry date is 3 months.
Buffer TE40 [2] TE40 [2] TE40 [2]
Storage 2-8°C -20°C -20°C
HK-GFP-1 v3.1
www.dynamic-biosensors.com
Page 1 of 4
Workflow of a heliX® GFP – capture assay
1. The anchor strand (ssDNA) immobilized to the surface of the heliX® Adapter Chip is hybridized with complementary DNA strands modified with GFP binding protein.
2. The GFP-tagged ligand of interest is captured on the surface during the measurement run. 3. Measurement of the analyte binding kinetics or conformational change upon analyte binding. 4. Surface regeneration by injection of a high pH solution. Chip surface goes back to the original state. This step can be
followed by a new hyridization of fresh ligand with GFP binding protein.
Preparation
1. Mix 100 µL GFP binding protein – Ligand strand with 100 µL Adapter strand 1 – Ra. 2. Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization. 3. Mix 200 µL Adapter strand 2 – Ra – lfs to the sample after step 2.
The solution (400 µL in total) is ready to use for a biochip functionalization.
Please aliquot and store the ready to use solution at 2-8°C. Use up within 3 months.
The kit contains material for the preparation of two separate ready to use solutions with 400 µL each.
Assay Setup in heliOS
Go to heliOS > create a New Assay Workflow > add Custom Assay > load Kinetics with Capture > modify the parameters based on your needs and run the assay.
Suggested assay parameters (e.g., flow rate, time, LED power, etc.) are within the heliOS assay.
For further questions, please contact the support team at support@dynamic-biosensors.com
HK-GFP-1 v3.1
www.dynamic-biosensors.com
Page 2 of 4
Useful Order Numbers
Table 2. Order Numbers Product Name heliX® Adapter Chip 10x Passivation solution Regeneration solution
Comment Chip with 2 detection spots For passivation of chip surface For regeneration of chip surface
Order No ADP-48-2-0 SOL-PAS-1-5 SOL-REG-1-5
HK-GFP-1 v3.1
www.dynamic-biosensors.com
Page 3 of 4
Contact
Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany
Bruker Scientific LLC 40 Manning Road, Mamming Park Billerica, MA 01821 USA
Order Information order@dynamic-biosensors.com Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany. ©2025 Dynamic Biosensors GmbH For Research Use Only. Not for use in clinical diagnostic procedures.
HK-GFP-1 v3.1
www.dynamic-biosensors.com
Page 4 of 4
Documents / Resources
 |
Dynamic Biosensors HK-GFP-1 GFP Capture Kit [pdf] User Manual HK-GFP-1 GFP Capture Kit, HK-GFP-1, GFP Capture Kit, Capture Kit |