Dynamic Biosensors Adapter Strand 2 User Manual

Key Features

Adapter strand 2 for functionalization of heliX® Adapter Chip Spot 2.
Compatible with heliX® Adapter Chip.
Includes Adapter strands for 50 regenerations.
Ideal for MIX&RUN sample preparation.
Adapter strand 2 carries a moderately hydrophilic red dye (Ra) with a single positive net charge.

heliX® Adapter Chip Overview

The heliX® Adapter Chip features 2 spots with 2 different anchor sequences for DNA-encoded addressing. The diagram illustrates the chip structure: each spot has a fixed anchor strand. Spot 1 has an exchangeable Adapter strand 1, and Spot 2 has an exchangeable Adapter strand 2. The diagram also indicates the presence of a dye and a ligand associated with the adapter strands, and shows that the ligand strand is exchangeable.

Product Description

Order Number: AS-2-Ra-lfs

Table 1. Contents and Storage Information

Material	Cap	Concentration	Amount	Buffer	Storage
Adapter strand 2 - Ra - lfs	White	200/250 nM	5 x 200 μL	TE40 [1]	-20°C

For research use only.

This product has a limited shelf life; please see expiry date on label.

To avoid many freeze-thaw cycles, please aliquot the nanolever.

Preparation | MIX&RUN

In-solution hybridization of adapter and ligand strands:

Mix Adapter strand 1 - Ra (400 nM) and conjugated Ligand strand (500 nM) at a 1:1 ratio (v/v).
Incubate the solution of step 1 at Room Temperature (RT) at 600 rpm for 30 min to ensure complete hybridization.
Mix the solution from step 2 and Adapter strand 2 - Ra - Ifs (200 nM) at a 1:1 ratio (v/v).

The solution is ready to use for biochip functionalization.

Stability of the solution is related to the stability of the ligand molecule.

Table 2. Additional material for functionalization of spot 1 and reference spot 2.

Material	Concentration	Buffer	Related Product Name	Order No
Adapter strand 1 - Ra	400 nM	TE40 [1]	Adapter strand 1 with red dye Ra	AS-1-Ra
Ligand strand carrying the conjugated ligand	500 nM	PE40 [2]	heliX® Amine Coupling Kit 1	HK-NHS-1

Example

Required volume for 3 functionalizations: 100 μL with a final concentration of 100 nM.

	Vial 1	Vial 2
Adapter strand 1 - Ra (400 nM)	25 μL
Conjugated Ligand strand (500 nM)	25 μL
Adapter strand 2 - Ra - Ifs (200/250 nM)		50 μL

After incubation time, mix Vial 1 and Vial 2 to obtain 100 μL of ready-to-use DNA solution.

Contact Information

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany

Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA

Order Information: order@dynamic-biosensors.com

Technical Support: support@dynamic-biosensors.com

www.dynamic-biosensors.com

Instruments and chips are engineered and manufactured in Germany.

Footnotes:

[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
[2] If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.

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