1. Introduction
This manual provides detailed instructions for the installation, configuration, and maintenance of your ASUS TUF Gaming B650EM-E WiFi mATX Motherboard. This motherboard is designed to support AMD Socket AM5 processors and DDR5 memory, offering advanced features for computing systems.

Image: The ASUS TUF Gaming B650EM-E WiFi mATX Motherboard shown alongside its retail packaging and included WiFi antenna.
2. Safety Information
Observe the following safety precautions during installation and operation:
- Always disconnect the power cable from the wall socket before touching any internal components.
- Wear an anti-static wrist strap or frequently touch a grounded object to prevent electrostatic discharge (ESD) damage.
- Handle components by their edges to avoid touching sensitive parts.
- Ensure proper ventilation within your computer case to prevent overheating.
3. Package Contents
Verify that all items are present in your motherboard package:
- ASUS TUF Gaming B650EM-E WiFi mATX Motherboard
- WiFi Antenna
- User Manual and Documentation
- M.2 SSD Screws and Standoffs
- SATA Data Cables (quantity may vary)

Image: The ASUS TUF Gaming B650EM-E WiFi Motherboard displayed with its box, WiFi antenna, manual, and M.2 mounting hardware.
4. Motherboard Layout
Familiarize yourself with the key components and connectors on your motherboard.

Image: A top-down view of the ASUS TUF Gaming B650EM-E WiFi Motherboard, highlighting the CPU socket, DIMM slots, PCIe slots, and M.2 slots.
4.1. Rear I/O Panel
The rear I/O panel provides external connectivity for peripherals.

Image: A detailed view of the motherboard's rear I/O panel, showing ports for USB, Ethernet, DisplayPort, HDMI, audio, and WiFi antenna connectors.
- DisplayPort / HDMI: Video output ports.
- USB Ports: Various USB 2.0, 5Gbps, and 10Gbps Type-C/Type-A ports.
- 2.5Gb Ethernet: Network connectivity.
- WiFi 6 Antenna Connectors: For wireless network access.
- Audio Jacks: For audio input and output.
- BIOS FlashBack Button: For updating BIOS without a CPU or RAM.
5. Setup and Installation
Follow these steps to install your motherboard and essential components.
5.1. CPU Installation
- Open the CPU socket lever.
- Align the triangular mark on the CPU with the mark on the socket.
- Gently place the CPU into the socket. Do not force it.
- Close the socket lever to secure the CPU.
5.2. Memory (DDR5) Installation
- Open the clips at both ends of the DIMM slot.
- Align the notch on the DDR5 memory module with the key in the DIMM slot.
- Insert the module firmly until the clips snap into place.
5.3. M.2 SSD Installation
The motherboard features one PCIe 5.0 M.2 slot and two PCIe 4.0 M.2 slots.

Image: A close-up view of an M.2 slot on the motherboard, showing the M.2 Q-Latch mechanism.
- Remove the M.2 heatsink and protective film.
- Insert the M.2 SSD into the slot at a 45-degree angle.
- Push down the M.2 SSD and secure it with the M.2 Q-Latch mechanism or screw.
- Reattach the M.2 heatsink.
5.4. Expansion Card Installation (PCIe)
The motherboard includes a PCIe 5.0 x16 slot.
- Align your expansion card with the PCIe slot.
- Press down firmly until the card is seated and the PCIe Slot Q-Release mechanism engages.
- Secure the card to your chassis with a screw.
5.5. Power Connections
Connect the 24-pin ATX power connector and the 8+4 pin ProCool CPU power connectors from your power supply to the motherboard.
5.6. Front Panel Connections
Connect your chassis' front panel cables (power button, reset button, USB, audio) to the corresponding headers on the motherboard. Refer to the motherboard layout for header locations.
6. Operating Instructions
6.1. BIOS/UEFI Setup
To enter the BIOS/UEFI setup utility, press the DEL key or F2 key during system startup. The BIOS allows you to configure system settings, boot order, and hardware parameters.
6.2. Software and Drivers
Install the necessary drivers from the ASUS support website or the included support DVD (if applicable) for optimal performance. ASUS DriverHub provides a centralized platform for driver management. Utilize ASUS-exclusive Aura Sync RGB lighting for personalization.
7. Maintenance
7.1. BIOS Updates
Regularly check the ASUS support website for the latest BIOS updates. Updates can improve system stability, compatibility, and performance. The BIOS FlashBack feature allows for convenient BIOS updates.
7.2. Cleaning
Periodically clean your motherboard and PC components to prevent dust buildup, which can affect cooling and performance. Use compressed air and anti-static brushes. Ensure the system is powered off and unplugged before cleaning.
8. Troubleshooting
8.1. Q-LED Indicators
The motherboard features Q-LED indicators that provide visual cues for troubleshooting during startup. Refer to the manual for specific LED codes:
- CPU LED: Indicates CPU initialization issues.
- DRAM LED: Indicates memory detection or initialization issues.
- VGA LED: Indicates graphics card detection or initialization issues.
- BOOT LED: Indicates issues with the boot device.
8.2. Common Issues
- No Display: Check power connections to the graphics card and monitor. Ensure the graphics card is properly seated.
- System Instability: Verify memory compatibility and seating. Check CPU and chipset temperatures.
- BIOS FlashBack Failure: Ensure the USB drive is formatted to FAT32 and the BIOS file is correctly renamed.
9. Specifications
| Feature | Specification |
|---|---|
| Brand | ASUS |
| Series | TUF GAMING B650EM-E WIFI |
| Model Number | TUFGAMINGB650EM-EWIFI |
| CPU Socket | Socket AM5 |
| Compatible Processors | Ryzen™ 9000 & 8000 & 7000 Series Desktop Processors |
| Chipset Type | AMD B650 |
| RAM Memory Technology | DDR5 |
| Memory Clock Speed | Up to 8000 MHz (OC) |
| Memory Storage Capacity | 256 GB (Max) |
| PCIe Slots | 1x PCIe 5.0 x16, other slots as per mATX standard |
| M.2 Slots | 1x PCIe 5.0 M.2, 2x PCIe 4.0 M.2 |
| Ethernet | Realtek 2.5Gb Ethernet |
| Wireless Connectivity | Wi-Fi 6 |
| USB Ports | Rear USB 10Gbps Type-C, Front USB 5Gbps Type-C, various USB Type-A |
| Video Output | DisplayPort, HDMI™ |
| Form Factor | mATX |
| Product Dimensions | 9.6 x 9.6 x 1.9 inches |
| Operating System Support | Windows 11, Windows 10 64-bit |
10. Warranty and Support
For warranty information, please refer to the warranty card included with your product or visit the official ASUS website. For technical support, driver downloads, and further assistance, please visit the ASUS Store or the official ASUS support portal.





